ANNALES BOGORIENSES
Vol 19, No 1 (2015): Annales Bogorienses

In Vitro Induction Of Tetraploid Pummelo ’Nambangan’ (Citrus maxima (Burm.) Merr.) By Colchicine Treatment Using Germinated Seed, Shoot Tip And Cotyledonary Node As Explants

Wulandari, Dyah Retno (Unknown)
Purwito, Agus (Unknown)
Susanto, Slamet (Unknown)
Husni, Ali (Unknown)
Ermayanti, Tri Muji (Unknown)

Article Info

Publish Date
26 Jun 2015

Abstract

Tetraploid citrus are important for interploidal hybridization to create triploid seedless citrus.  Colchicine is the most commonly used as antimitotic agent to induce polyploid plants.  Tetraploid induction by colchicine in Pummelo ‘Nambangan’ was conducted in vitro using different types of explants.  The aim of this research was to induce tetraploid pummelo ‘Nambangan’ by colchicine treatment using germinated seed, shoot tip and cotyledonary node as explants.  Tetraploid shoot induction was conducted by soaking germinated seeds, shoot tips and cotyledonary nodes in 0.1% colchicine for 1, 3 and 5 h.  Regenerant shoots were grown on MS medium and their growth was observed after four weeks in culture.  Ploidy level was determined using flow cytometry analysis.  Stomata density, length and width of stomatal guard cell were also recorded. The results showed that shoot elongation was inhibited by colchicine treatment.  Soaking of shoot tip explants in 0.1% colchicine for 1 h resulted in 66.66% of putative tetraploid shoots.  Compared to diploid shoots, tetraploids had lower stomata density but bigger in guard cell size. Keywords: colchicine, tetraploid, pummelo (Citrus maxima (Burm.) Merr.), flow cytometry, stomata,

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Journal Info
ANNALES BOGORIENSES
Website

Abbrev

annales

Publisher

Lembaga Ilmu Pengetahuan Indonesia

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

Description

The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as ...