Medical Laboratory Technology Journal
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.
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<![CDATA[Detection of Salmonella typhi Using Multiplex and Monoplex PCR in Tifoid Fever Patients ]]>
<![CDATA[Andika Aliviameita]]>;
<![CDATA[Meryn Linanda Agustin]]>;
<![CDATA[Anggie Wedha Puspita]]>;
<![CDATA[Miftahul Mushlih]]>;
<![CDATA[Puspitasari Puspitasari]]>;
<![CDATA[Yanik Purwanti]]>;
<![CDATA[Arief Wisaksono]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.230
<![CDATA[Typhoid fever is a disease caused by Salmonella typhi infection. This disease is still a global problem. The purpose of this study was to develop a rapid detection method for Salmonella using molecular methods utilizing Inv A and Fim-C Genes. Two methods compared, which are Multiplex and monoplex PCR. The sample is in the form of the patient's blood, which is stored at 3 ml EDTA vacutainer tube consisting of 10 positive samples and five negative samples. The criteria for samples used previously have tested by widal/ immunoserology (titer more than 1/160). The results showed that multiplex PCR for detection of Salmonella using the Inv A gene and fim c gene not recommended because too many bands produced. The application of monoplex in the Inv A gene gives better results than the Fim C gene. In conclusion, the monoplex application on Inv A gene recommended than Fim C gene used to detect S. typhi in human blood samples.]]>
<![CDATA[Analysis between Adrenal MRI Mass Size and 1 mg Dexamethasone Suppression Test in Patients with Adrenal Incidentaloma ]]>
<![CDATA[Pinar Karakaya]]>;
<![CDATA[Bulent Yaprak]]>;
<![CDATA[Bahar Ozdemir]]>;
<![CDATA[Hakan Kocoglu]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.223
<![CDATA[This study aimed to investigate the correlation of adrenal magnetic resonance imaging (MRI) mass size and 1 mg dexamethasone suppression test results in patients with adrenal incidentaloma diagnosis. Eighty-six patients (82.6% female) with incidentally discovered adrenal masses, diagnosed and treated in the Department of Endocrinology Bakirkoy Dr. Sadi Konuk Education and Research Hospital between June and August 2017, were included in the analysis. All the patients evaluated for their hormonal functionality. 24-h urinary excretion of cortisol did not correlate with tumor size. But there was a positive correlation between longer adrenal nodule diameter and metanephrine, and there was a negative correlation between shorter adrenal nodule diameter and renin levels. We found that smaller and clinically “silent” tumors often do not demonstrate subclinical hormonal activity.]]>
<![CDATA[Analysis between Platelet Count and Blood Groups in Apheresis Platelet Donors with Demographic Features ]]>
<![CDATA[Canan Eren]]>;
<![CDATA[Serpil Çeçen]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.250
<![CDATA[Platelet transfusion is used to prevent bleeding in patients with thrombocytopenia or platelet dysfunction. Purpose of investigating demographic characteristics of eligible volunteers as platelet donors and of demonstrating the association of platelet counts with blood groups as well as other factors. We used data of individuals who referred to a blood center in our hospital. Age, body weight, body mass index (BMI), and gender were determined, and than hemogram values such as leukocyte, hemoglobin, hematocrit, and platelet and ABO blood types of those individuals were identified. A statistically significant difference determined for height, body weight, and BMI in both genders. Although BMI was lower in the group of platelet count ≤ 250, it was higher in a group of platelet count > 250. Furthermore, platelet count was more moderate in blood group O Rh-positive but, no significant difference was group O Rh-negative. Platelet count was higher in other Rh-positive blood groups than Rh negatives. BMI is vital in apheresis donors, and individuals with higher BMI values may prefer in case of double dose or more apheresis need. The determination of platelet counts in Rh positives is more elevated than Rh negatives.]]>
<![CDATA[Acute Toxicity Test of Black Pomegranate Peel Extract (Granati Fructus Cortex) Against Larvae of Shrimp (Artemia salina Leach) ]]>
<![CDATA[Rofiatu Sholihah Achmad]]>;
<![CDATA[Lingga Aditya Aditya]]>;
<![CDATA[Nosa Ika Cahyariza]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.242
<![CDATA[Toxicity test on red pomegranate has done, while in black pomegranate is not widely known. Black pomegranate peel (Granati Fructus Cortex) has some chemical ingredients such as saponins, flavonoids, tannins, and alkaloids. Alkaloids, saponins, and flavonoids are thought to be toxic at certain levels. This study aimed to determine the effects of toxic black pomegranate peel extract (Granati Fructus Cortex) against larvae of shrimp (Artemia salina Leach) indicated LC50 values below 1000 µg / ml. This study is purely experimental by using Brine Shrimp Lethality Test (BSLT). The experiments divided into five groups, namely ethanol extract of black pomegranate peel (Granati Fructus Cortex) with a concentration of 100, 150, 200, 250 ppm, and negative control (seawater). Mortality data percentage of Artemia salina Leach analyzed by probit analysis. Results showed that the extract of black pomegranate peel (Granati Fructus Cortex) has a toxic effect on larvae of Artemia salina Leach with LC50 values of 114.090 µg/ml. From these results, it can conclude that black pomegranate peel extract has a potential acute toxic to larval shrimp (Artemia salina Leach) with Brine Shrimp Lethality Test (BSLT) method.]]>
<![CDATA[Young and Old Sugar Apple (Annona squamosa Linn) Leaf Extracts As an Aedes aegypti Larva Insecticide ]]>
<![CDATA[Abdul Khair]]>;
<![CDATA[Noraida Noraida]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.231
<![CDATA[Vector-borne diseases are still a health problem in some tropical countries. One vector-borne disease is Dengue Hemorrhagic Fever (DHF). DHF never decreases and even tends to continue to increase, and many cause deaths in children, 90% of them attack children under 15 years. DHF cases in 2018 amounted to 65,602 cases, with 467 deaths (CFR = 0.71%). The behavior of holding water in various places such as tubs and tendons indirectly creates a breeding place for Aedes aegypti mosquitoes. The use of chemicals as insecticides can cause the death of non-target animals, environmental pollution, and the occurrence of vector resistance to insecticides. Therefore it is necessary to do other methods including the use of vegetable insecticides. Sugar apple leaves can be used to kill Aedes aegypti larvae. Sugar apple leaves will obtain throughout the year. The purpose of this study is to know the ability of young and old sugar apple leaves to kill Aedes aegypti larvae. This type of research is a posttest only control group design. The study sample was part of the Aedes aegypti larvae on the final instar III. The experiment to kill Aedes aegypti larvae use two types of sugar apple leaf extract, namely young and old sugar apple leaves with nine treatments four replications. Each treatment consisted of 25 larvae. The research results show There was no significant difference in the number of dead larvae using either extracts from young sugar apple leaves or old sugar apple leaves. LC90 of sugar apple leaf extract was between 0.05632 to 0.08324% and the effective residual age at LC90 (0.06568%) with the death of Aedes aegypti larvae was 92% over 24 hours (1 day).]]>
<![CDATA[Quality of Genom in Type II Diabetes Mellitus Patients in Viewed of Temperature, Storage Duration, Number of Leukocyte ]]>
<![CDATA[Puspitasari Puspitasari]]>;
<![CDATA[Evi Rinata]]>;
<![CDATA[Rohman Dijaya]]>;
<![CDATA[Siska Aprilia]]>;
<![CDATA[Dina Trikumalasari]]>;
<![CDATA[Livia Nur Azzah]]>;
<![CDATA[Qilmia Fanani]]>;
<![CDATA[Miftahul Mushlih]]>;
<![CDATA[Andika Aliviameita]]>;
<![CDATA[Dian Delta]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.229
<![CDATA[Type II Diabetes Mellitus is a disorder caused by genetic and environmental factors. Molecular analysis of T2DM abnormalities has been carried out. But the analysis in sample preparation especially the stages of DNA isolation has not been done much. The aims of this study are to investigate the effect of temperature, storage duration and level of white blood cell (WBC) with Genome Quality in T2DM. The treatment (n:10) which were divided into several tubes and then stored at 4 °Ϲ, 25 °Ϲ and 32 °Ϲ for 21 days. To determining storage duration effect, we use periodically isolation of DNA (3, 15 and 30 days) after sampling. The effect of WBC with DNA quality was carried out using 17 samples. DNA isolation was done by the DNA Isolation Kit manual without modification and then tested qualitatively and quantitatively. Based on this research, it can be concluded there is a correlation between the numbers of WBC with DNA quality. The higher the number of WBC, the higher DNA concentration (r: 0.818. p value: 0.000). The concentration of DNA at a temperature of 4°Ϲ (135.1 ± 165.2 ng / µl) was higher compared with the temperature treatment 25 ° Ϲ (29.7 ± 36.5 ng / µl) and 32 ° Ϲ (22.14 ± 7.13 ng / µl) (p]]>
<![CDATA[Anti-hyperglycemic Effect of Torbangun (Coleus amboinicus Lour) Leaves Extract Through Liver and Muscle Glycogen Deposits in Streptozotocin-induced Hyperglycmic Sprague-Dawley Rats Model ]]>
<![CDATA[Meilla Dwi Andrestian]]>;
<![CDATA[Rizal Damanik]]>;
<![CDATA[Faisal Anwar]]>;
<![CDATA[Nancy Dewi Yuliana]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.227
<![CDATA[The association of liver and muscle glycogen deposits with serum insulin levels, β-cells pancreas, and fasting blood glucose (FBG) of streptozotocin (STZ)-induced hyperglycemic rats receiving Torbangun leaves extract (TE) investigated. The intervention performed on 25 8-week-old Sprague-Dawley rats divided into four groups. Seven rats separated as a normal group (N), and other rats injected with streptozotocin (STZ). Confirmation of hyperglycemic was characterized by fasting blood glucose >126 mg/dl. Treatment group which is NG (hyperglycemic rats); N (normal rats); H-IM (62.5 mg/kg BW metformin); and H-IT (620 mg/kg BW TE) for 14 days. This study revealed that TE significantly decreased FBG levels, increased insulin production, and the amount of liver glycogen deposits (a=0.01). However, the intervention did not significantly increase the amount of muscle glycogen deposits. TE administration improves β-cells, increases the liver and muscle glycogen deposits. TE was shown to have antihyperglycemic activity by improving the β-cell, increasing blood serum insulin levels, decreasing blood glucose levels, and increasing the liver glycogen deposits.]]>
<![CDATA[Ethanol Extract of Black Cumin Seed (Nigella Sativa) Reduces Expression Kidney iNOS in Mice Model Preeclampsia ]]>
<![CDATA[Rubiati Hipni]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.247
<![CDATA[This study aims to analyze the effect of ethanol extract of black cumin seeds (Nigella Sativa) on iNOS expression in the kidney of mice model preeclampsia. Design This study uses an experimental design with a posttest only control group design. Using a mice model preeclampsia. This study was divided into 6 groups: negative control, positive control (preeclampsia model), model + black cumin seed extract 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day days, after surgery on mice then examined iNOS Kidney expression by methods Immunohistochemical. Data from observations were analyzed by one way ANOVA test. The result there is an effect of ethanol extract of black cumin seeds on renal iNOS expression in mice model preeclampsia (p]]>
<![CDATA[Analysis of Obesity And Axillary Lymph Nodes on Mammography ]]>
<![CDATA[Aysegul Akdogan Gemici]]>;
<![CDATA[Ersoy Bayram]]>;
<![CDATA[Nigar Gultekin]]>;
<![CDATA[Ercan Inci]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.235
<![CDATA[Fat-infiltrated lymph nodes mostly seen on mammograms (MG) of the elderly and obese women. We analyzed the fat infiltration of lymph nodes for its potential use in estimating body mass index (BMI). 2D digital MG of 157 women assessed by one breast radiologist and one radiology resident. For each patient, the largest lymph node chosen as the index by analyzing the medial, lateral oblique (MLO) views. The width and length of the node and the hilum, the cortex, and the Hilo-cortical ratio (HCR) were measured. Breast density was categorized according to the ACR BI-RADS 4th edition and divided into two groups as dense and non-dense. Obesity was defined as BMI ≥ 30 kg/m2, overweight as BMI]]>
<![CDATA[Detection of Mycobacterium tuberculosis by Microscopic Technique of Sputum AFB and ICT (Cocktail Antigen) TB ]]>
<![CDATA[Retno Martini Widhyasih]]>;
<![CDATA[Annisa Husnun Hanifah]]>;
<![CDATA[Chairlan Chairlan]]>;
<![CDATA[Dewi Inderiati]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.246
<![CDATA[Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. The diagnosis of pulmonary TB in general still relies on microscopic examination with a sputum smear for Acid Fast Basil (AFB). Microscopic examination to detect M. tuberculosis has high specificity, but the sensitivity is 35-70%. The sputum culture method is the gold standard for the diagnosis of pulmonary TB but requires a long time, which is 6-8 weeks. In recent years ICT (Immuno Chromatography Test) has been introduced to identify potential and useful TB antigens to help diagnose pulmonary TB. Antigens detected were ESAT-6, CFP-10, CFP-21, and MPT-64. This study aims to compare the results of the examination of specimens of suspect TB sputum with the ICT method and AFB. This study based on observational with analytic design cross-sectional on 56 samples of Stored Biological Material suspects TB that were examined by the AFB method and the ICT method (Cocktail Antigen). Statistical test results Fisher Exact (α = 0.05) showed no difference between microscopic examination of smear sputum and ICT (Cocktail Antigen) TB (p = 1,000). Nevertheless, the use of ICT reagents for the diagnosis of pulmonary TB still needs to be further investigated, especially to find out the exact causes of false positive and false negative reactions.]]>
