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Jurnal Ilmu Nutrisi dan Teknologi Pakan

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Agriculture, Biological Sciences & Forestry

Artikel yang dipublikasikan pada Jurnal Ilmu Nutrisi dan Teknologi Pakan mencakup cakupan luas topik penelitian dalam bidang ilmu nutrisi dan teknologi pakan, termasuk agrostologi, industri dan teknologi pakan, nutrisi ternak unggas, nutrisi ternak pedaging, nutrisi ternak kerja dan olahraga, nutrisi ternak perah, nutrisi satwa harapan dan hewan peliharaan, mikrobiologi rumen, mikrobiologi ternak, fisiologi nutrisi, metabolisme nutrisi, kajian biologi molekuler terkait nutrisi pakan, ternak dan metabolismenya, dan kajian-kajian lain yang relevan dengan topik dan isu terkini dalam bidang ilmu nutrisi dan teknologi pakan.

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Articles 96 Documents

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Pengaruh proses steam pada daun kelor (Moringa oleifera) dan asam fulvat terhadap performa ayam broiler H A Sukria; IES Nugraha; D M Suci
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 2 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh proses steam pada daun kelor (Moringa oleifera) terhadap performa ayam broiler. Sebanyak 120 DOC di bagi ke dalam 5 perlakuan ransum dengan 3 ulangan yang dipelihara selama 35 hari. Pakan yang diberikan terdiri dari dua periode yaitu starter (protein kasar 22,07% dan energi metabolis 305,65 kkal kg-1) dan finisher (protein kasar 20,3% dan energi metabolis 3120 kkal kg-1). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari lima perlakuan ransum yaitu: P0 (Control), P1 (Control + 5% daun kelor tanpa steam), P2 (Control+5% daun kelor tanpa steam + 0,5% asam fulvat), P3 (Control+5% daun kelor steam), dan P4 (Control+5% tepung daun kelor steam dan 0,5% asam fulvat). Data yang diperoleh dianalisis dengan Analysis of Variance (ANOVA) dan uji Duncan Multiple Range Test. Hasil penelitian menunjukkan penambahan daun kelor dan asam fulvat pada ransum mempengaruhi bobot badan ayam broiler (P<0,01). Penambahan daun kelor steam menghasilkan bobot badan akhir ayam broiler yang sama jika dibandingkan dengan perlakuan kontrol. Penambahan daun kelor steam dengan asam fulvat (P4) menghasilkan bobot badan akhir yang lebih rendah dibandingkan perlakuan dengan asam fulvat tanpa steam (P2 dan P3)(P<0,01). Berdasarkan hasil tersebut menunjukan bahwa asam fulvat yang dipanaskan memberikan pengaruh yang negatif terhadap performa ayam broiler.Kata kunci: asam fulvat, daun kelor, kakas, performa, proses steamABSTRACTThe research was conducted to investigate the effect of hydro thermal process (steam) of Moringa leaf meal and fulvic acid on performance of broiler chicken. The120 heads of Day Old Chicken (DOC) were used in this experiment. A completely randomized design was used in this experiment with 5 treatments and 3 replications with 8 broilers in each experimental unit and was reared for 35 days. The treatments were P0 (Control), P1 (Control+5% steamed Moringa leaf), P2 (Control+5% unsteamed Moringa leaf meal and 0.5% fulvic acid), P3 (Control+5% steamed Moringa leaf meal), and P4 (Control+5% steamed Moringa leaf meal and 0.5% fulvic acid). All data were analysed by analysis of variance and Duncan multiple ranges. The result of this research showed that Moringa leaf meal and fulvic acid significantly affected (P<0.01) broiler performance. The addition of Moringa leaf meal with steamed process did not significantly affect on final body weight compared to the control. Meanwhile the addition of Moringa leaf powder and fulvic acid with steamed process resulted in significantly the lower final body weight of the broiler compared to the treatments of fulvic acid without steam process. From this result can be concluded that that steam treatment of fulvic acid result in negative impact on the broiler performance.Key words: carcass, fulvic acid, Broiler performance, Moringa leaves, steam process

Evaluasi produksi dan kualitas inokulum fungi mikoriza arbuskula yang diproduksi dengan teknik hidroponik pada rumput Brachiaria decumbens var. mullato A T Aryanto; P DMH Karti; I Prihantoro
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 2 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

ABSTRAKPengembangan hijauan membutuhkan pupuk ramah lingkungan. Fungi Mikoriza Arbuskular (FMA) adalah asosiasi yang melibatkan jamur dan akar yang dianggap sebagai pupuk hayati untuk meningkatkan produktivitas tanaman dan toleran dari kondisi lingkungan. Ketersediaan FMA masih jarang, sehingga dibutuhkan produksi massal untuk dapat mendukung pengembangan hijauan. Tujuan dari penelitian ini adalah untuk menghasilkan inokulum AMF menggunakan sistem hidroponik dalam jumlah besar secara efektif. Penelitian ini dibagi menjadi 2 tahap. Rancangan Acak Kelompok Faktorial yang digunakan pada tahap pertama dengan faktor A adalah jenis sistem irigasi (Manual, Drip dan Nutrien Film Technique System (NFT)) dan B adalah larutan nutrisi (AB Mix dan Hyponex Red) dengan Pueraria javanica sebagai tanaman inang. Rancangan Acak Lengkap (RAL) dilakukan pada tahap kedua dengan menggunakan produksi inokulum FMA dari tahap pertama dengan Brachiaria decumbens var Mullato sebagai tanaman inang. Hasil penelitian menunjukkan bahwa interaksi antara sistem NFT dan AB Mix signifikan (P<0,05) menghasilkan bahan kering tajuk, bahan kering akar dan spora paling tinggi. Semua tipe sistem irigasi dan nutrisi menunjukkan infeksi akar>96%. FMA inokulasi di Brachiaria decumbes var Mulato signifikan(P <0,05) pada bahan kering tajuk, kandungan N, kandungan P dan serapan P.Kata kunci: Brachiaria decumbes, FMA, sistem Drip, sistem NFT, Pueraria javanica,ABSTRACTForage mass production development requires environmental friendly fertilizer. Arbuscular Mycorrhizal Fungi (AMF) are mutualitic symbioses between plant and fungi that considered as natural biofertilizer with benefit to improve plant productivitity and environment stress tolerance. The availability of AMF is still low, so it takes mass production to be able to support forage development. The aim of the research was to produce AMF inoculum using hydroponic system in large quantities. This research divided into 2 stages. The factorial randomized block design was used for the first stage with A factor was type of irrigation system (Manual, Drip and Nutrient Film Technique System (NFT)) and B was the nutritional solution (AB Mix and Hyponex Red) using Pueraria javanica as host plant. Completely randomized design was conducted for the second stage by using AMF inoculum production from first stage using Brachiariadecumbens var Mullat as host plant. The best result was a combination beetwen NFT system and AB Mix significantly (P<0.05) produce highest shoot dry matter, root dry matter and spore production. All type of irrigation system and nutrition showed root infection >96%. AMF inoculation in Brachiariadecumbes var Mulato was significant different (P<0.05) on shoot dry matter, N content, P content and P uptake.Keywords: AMF, B. decumbes, Drip system, NFT system, P. javanica,

Karakteristik produk dan efektivitas enkapsulasi bakteri pendegradasi asam sianida (HCN) A Mislah; S Suharti; I Wijayanti
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 2 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

ABSTRAKDaun singkong pahit (Manihot esculenta)memiliki kandungan antinutrisi berupa asam sianida (HCN) yang tinggi, namun HCN dapat di degradasi dengan bakteri rumen. Penelitian ini bertujuan untuk mengamati karakteristik produk dan efektivitas enkapsulasi bakteri pendegradasi sianida dengan masa simpan (0, 1, 2, 3, 4, 5, 6, 7, 14, 21, 28 hari) pada suhu ruang. Peubah yang diamati adalah karakteristik produk dan viabilitas bakteri pendegradasi sianida terenkapsulasi. Data yang diperoleh dianalisa dengan sidik ragam (ANOVA) menggunakan program SPSS 16.0. Hasil penelitian menunjukkan bahwa lama penyimpanan berpengaruh terhadap perubahan warna dan bentuk dari produk hasil enkapsulasi. Penyimpanan produk enkapsulasi bakteri pendegradasi sianida selama 3 hari tidak mempengaruhiviabilitas bakteri dibandingkan kontrol. Namun demikian semakin lama penyimpanan produk enkapsulasi bakteri sampai hari ke 28 nyata menurunkan (P<0.05) viabilitas bakteri.Hasil penelitian dapat disimpulkan bahwa dengan teknik enkapsulasi dapat mempertahankan viabilitas bakteri pendegradasi sianida.Kata kunci: bakteri pendegradasi sianida, karakteristik produk, penyimpanan,viabilitasABSTRACTBitter cassava leaves have high antinutrients in the form of cyanide acid (HCN), but HCN can be degraded with rumen bacteria. This research aimed to observe the product characteristics and the effectivity of cyanide degradation bacteria capsulation with different length of storages (0, 1, 2, 3, 4, 5, 6, 7, 14, 21, 28 days)in the room temperature. The observed variables were product characteristics and viability of cyanide degrading bacteriacapsulation. The data obtained were analyzed by Analysis of variance (ANOVA) using SPSS 16.0 program. The results showed that storages duration affected the color and shape of cyanide degradation bacteria capsulation products. The storage of capsulated HCN degrading bacteria up to 3 days did not affect the viability of bacteria compared to the control treatment. However, the longer storage of capsulated bacteria up to 28 days, significant decreased (P<0.05) the viability of the bacteria. It is concluded that capsulation of cyanide degrading bacteria could maintain the viability of bacteria.Keywords: cyanide degradation bacterial, product characteristics, storage, viability

Acidifier alami air perasaan jeruk nipis (Citrus aurantiun) sebagai pengganti antibiotik growth promotor ayam broiler K Hidayat; S Wibowo; L A Sari; A Darmawan
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 2 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

ABSTRAKPenelitian ini bertujuan untuk mengevaluasi pemberian air jeruk nipis dalam air minum sebagai pengganti antibiotic growth promotor terhadap performa dan populasi mikroba usus halus ayam broiler. Ayam broiler yang digunakan dalam penelitian ini yaitu 90 ekor ayam broiler dipelihara dari umur 1 hari hingga umur 28 hari. Penelitian ini menggunakan rancangan acak lengkap dengan 3 perlakuan dan 3 ulangan. Perlakuan ini yaitu P0= Ransum + air minum tanpa perasan air jeruk nipis (kontrol), P1= Ransum + air minum dengan perasan air jeruk nipis hingga pH air minum menjadi 5, P2= Ransum + air minum dengan perasan air jeruk nipis hingga pH air minum menjadi 3. Peubah yang diamati dalam penelitian ini yaitu konsumsi pakan, pertambahan bobot badan, konversi pakan, populasi bakteri E. coli, populasi bakteri asam laktat, dan mortalitas. Hasil penelitian ini menunjukkan bahwa pemberian air perasan jeruk nipis dalam air minum tidak memberikan efek berbeda nyata pada pertambahan bobot badan, konsumsi ransum, konversi ransum, populasi E. coli, akan tetapi perlakuan memberikan efek berbeda nyata (P<0.05) pada populasi bakteri asam laktat yang terdapat pada digesta usus halus ayam broiler. Pemberian perasaan air jeruk nipis dapat meningkatkan efisiensi pakan sebesar 3,3 %.Kata kunci : Ayam broiler, acidifier, jeruk nipis, performa, E. coli, bakteri asam laktatABSTRACTThe purpose of this study was to evaluate the effect of lime juice in drinking water as antibiotic growth promoter substitute on broiler performance and intestine microbial population. This study used 90 broiler chickens that were reared up to 28 days. This experiment was designed as a completely randomized design with 3 treatments and 3 replications; P0= Diet + drinking water without lime juice (control treatment), P1= Diet + drinking water with lemon juice (pH=5), P2= Diet + drinking water with lime juice (pH =3). The results of this study showed that all treatments did not affect weight gain, feed consumption, feed conversion, E. coli population. The treatment of drinking water with lime juice at pH =3 significantly increased (P<0.05) lactic acid population. Utilization of lime juice in drinking water at pH 5 increase 3,3% of feed efficiency and reduce the mortality rate.Key words: lime juice, broiler perfomance, lactic acid bacteria

Efek suplementasi “Jamu Rempah” pada puyuh (Coturnix coturnix japonica) terhadap performa dan kadar kolesterol telur A Andari; E N Enisa; R F Wulandari; D M Suci
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 2 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

ABSTRAKTelur puyuh merupakan salah satu sumber protein hewani yang banyak disenangi masyarakat. Namun, sebagian orang membatasi untuk mengkonsumsinya karena kolesterol yang tinggi pada telur puyuh. Penelitian ini bertujuan untuk mengetahui taraf pemberian “Jamu Rempah” yang efektif dalam menurunkan kadar kolesterol telur puyuh. “Jamu Rempah” terdiri dari teh, kayu manis, kapulaga, bunga lawang, pala, dan cengkeh. Penelitian menggunakan rancangan acak lengkap 4 perlakuan dengan 4 ulangan. Puyuh (Coturnix coturnix japonica) yang digunakan sebanyak 128 ekor umur 42 hari yang dipelihara selama 5 minggu dan diberi ransum komersial. Perlakuan 1 (P0) kontrol atau tanpa “Jamu Rempah” dan perlakuan suplementasi jamu rempah 10 ml, 30 ml dan 50 ml dalam 1 liter air minum. Variabel yang diukur adalah konsumsi air minum, konsumsi pakan, produksi telur dan kolesterol telur. Hasil penelitian menunjukan bahwa suplementasi “Jamu Rempah” pada ternak puyuh dapat menurunkan kadar kolesterol telur puyuh. Pemberian “Jamu Rempah” memiliki efek yang signifikan (P<0,05) untuk meningkatkan konsumsi air minum burung puyuh (P<0,05) dan produksi telur puyuh. Perlakuan P1 (pemberian 10 ml “Jamu Rempah” dalam 1 liter air minum) dan P2 (pemberian 30 ml “Jamu Rempah” dalam 1 liter air minum) memiliki hasil yang sangat baik menurunkan kadar kolesterol telur puyuh.Kata kunci: jamu, kadar kolesterol, rempah, suplementasi, telur puyuhABSTRACTQuail eggs is one source of animal protein that many people like. However, some people decrease to consume it because of high cholesterol in quail eggs. This study aims to determine the level of herb medicine (Jamu Rempah) which is effective in lowering the cholesterol level of quail eggs. The ingredients of “jamu rempah” were tea, cinnamon, star anise, cardamom, nutmeg, and cloves. This study was conducted by using a completely randomized design with 4 treatments and 4 replications. The treatment were a control (PO) and supplementation of herb medicine (jamu rempah ) : 10 ml (P1), 30 ml (P2) and 50 ml (P3) in 1 liter of drinking water. One hundred and twenty eight quails aged 6 weeks used in study and reared for 5 weeks were feed by commercial ration. The variables observed were water consumption, feed consumption, eggs production and eggs cholesterol. The results showed that supplementation of herb medicine in quail can lower the level of eggs quail cholesterol. Supplementation of herb medicine has a significant effect to increase quail water consumption (P <0.05) and quail egg production (P <0.05).Treatment P1 (10 ml “Jamu Rempah” in 1 liter water) and treatment P2 (30 ml “Jamu Rempah” in 1 liter water) have excellent results t decrease the cholesterol level of quail eggsKeywords: jamu, cholesterol level, herb, supplementation, quail egg

Populasi Bakteri dan Keberadaan Bakteri gram (+) dan (-) dalam Pellet Calf Starter yang Ditambah Limbah Kubis Fermentasi S Mukodiningsih; B Sulistiyanto; SS Sholikhah
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 3 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

The aim of the experiment was to evaluate total bacteria population and the existence of positive and negative gram bacteria of pellet calf starter which added by lactic acid bacteria from fermented cabbage waste. The materials in this research were corn, rice bran, soybean meal, mineral mix, molasses, fermented cabbage wasted. The research was conducted by Completely Randomized Design with 4 treatments and 5 replications. The treatments were T0 (100% calf starter), T1 (2% of fermented cabbage waste + 100% calf starter), T2 (4% of fermented cabbage waste + 100% calf starter) and T3 (6% of fermented cabbage waste + 100% calf starter). The Variables observed were bacteria population and gram bacteria. Data of bacteria population were evaluated by descriptive analysis, while data of gram bacteria existence were statistically analyses by variance analysis, if there were significantly affect continued by Duncan test. The results showed that the highest bacteria population was on the treatment with addition level 2% of fermented cabbage waste and the lowest bacteria population was on addition level 6%. Adding fermented cabbage waste did not significantly effect to the existence of gram bacteria. The average score of gram bacteria existence on added fermented cabbage waste from level 0%, 2%, 4%, and 6% were respectively 1.99; 2.10; 2.29 and 2.29. Positive gram bacteria extended increased with increased fermented cabbage waste. The conclusion was the pellet calf starter which added with lactic acid bacteria produced bacteria and there was positive gram bacteria, and suppress negative bacteria. Keyword: bacteria population, fermented cabbage waste, gram bacteria, pellet calf starter

The Effect of Different Energy Level on The Ration to The Mating Acceleration of Local Sheep L Khotijah; A Maulidina; K B Satoto; I K G Wiryawan
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 3 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

The aim of this research was to find the appropriate ration energy levels to determine the puberty time of ewes. The study used 12 offspring female sheep obtained from crossing Jonggol ewes of Animal Science Teaching and Research Unit (JASTRU) Faculty of Animal Science IPB with Garut male sheep. The animal age was approximately 2-3 months with the average body weight 9.79 ± 1.97 kg. The treatments were consisted of 3 rations which have different TDN level, namely: T1= 65% TDN, T2=70% TDN and T3= 75% TDN. The experimental design used was a completely randomized design with three treatments and four replications. The measured parameters were feed intake, average daily gain, and the first matting age and body weight. The data obtained were analyzed using Analysis of Variance. The results indicated that the ration energy levels did not significantly affected on the feed intake, average daily gain, as well as the first mating age and body weight. The age of first mating was 180-203 days with body weight at 18-22 kg. It is concluded that the TDN level between 65% -75% has similar response to accelerate the mating of local sheep. Keywords: energy, local sheep, mating acceleration

Energy Balance and Blood Metabolites Status of Local Sheep Based on Indigofera sp and Sproutbean Waste Ration D A Astuti; S Rahayu; K B Satoto; R Priyanto; L Khotijah; T Suryati; M Baihaqi
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 3 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

This research was undertaken to evaluate energy utilization and blood metabolites status of male Indonesian local sheep involving nutrient balances and using urea space techniques for measurement body composition. Factorial randomized completely design was used in this study using 16 growing male local sheep. Factor A was breed which consisted of 8 Garut sheep (av.BW 14.90 kg) and 8 Jonggol sheep (av.BW 13.60 kg). Factor B was different ration which received of diet containing Indigoferasp. and sproutbean waste. The ration offered were pellets containing CP around 16% and energy 15.6 MJ GE kg-1. Energy balance and blood metabolites studies were conducted during three-months trial. Intake, digestibility, urinary and metabolism variables were measured based on total collection method. Methane energy was measured by rusitec technique and methane gas production multiplied by calory equivalent value of methane. Energy retention (RE) was measured by urea space technique. Heat production was calculated from ME-RE. Plasma glucose, cholesterol and urea-N were measured by using spectrophotometry. Results showed that there were significant differences of energy intakes, digestibilities and metabolism among the rations. Animals fed with sproutbean waste ration has higher energy utilization compared to those fed Indigofera sp ration. There were no significant different for all blood metabolite variables among breed and its interaction between combination treatments. It is concluded that sproutbean waste in local sheep ration has higher energy utilization and daily gain than Indigofera sp. Keywords: Indigofera sp., local sheep, rusitec technique, sproutbean, urea space

Pengaruh Pemberian Tepung Deffated Larva BSF (Hermetia illucens) terhadap Performa Produksi Puyuh Petelur (Coturnix coturnix japonica) S Mawaddah; W Hermana; N Nahrowi
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 3 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

High quality protein feedstuff prices for poultry are more expensive due to rising prices of imported raw materials, such as fish meal and meat bone meal (MBM). Utilization of an alternative source of protein that is abundant and does not compete with food needs, becomes one of the promising solutions to optimize production efficiency. This research will evaluate the potential utilization of black soldier fly (BSF) deffated larva mal as alternative source of MBM protein in quail feed on quail production performance by using method of decreasing fat (defatting) with emphasis (pressing). Female quail 5 weeks old as many as 285 birds. Quails devided in 3 treatments and 5 replications of each 19 tails. The experimental design used in this study was a complete randomized design (CRD). The feed applied was as follows: (P1) control feed, (P2) Feed with content 50% BSF (P3) feed with 100% BSF. The results of the study 50% and 100% subtitution of BSF deffated larva meal not significantly affected on egg production evidenced by no disruption of the production of eggs and raised 5.82% (P2) and 6.13% (P3) to feed control so that it can be concluded that deffated BSF meal alternative can be used as a substitute for MBM quail in feed. Keyword: Black soldier fly, deffated, quail, meat bone meal

Karakteristik Fermentasi Rumen In vitro dengan Penambahan Sabun Kalsium Minyak Nabati pada Buffer yang Berbeda S Suharti; D N Aliyah; S Suryahadi
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 3 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Canola oil and flaxseed oil are vegetable oils as potential sources of unsaturated fatty acid that could improve the production and quality of beef meat. However, the use of vegetable oils need to be protected to avoid biohydrogenation by rumen bacteria. The research was aimed to analyse effect of flaxseed/canola oils calcium soap in the different buffer media on in vitro fermentation characteristic. The experiment was conducted in a factorial randomized block design with 2 factors and 3 blocks based on rumen sampling time. The first factor was sources of vegetable oils (canola and falxseed) and the second factor was type of buffer (Kajikawa and McDougall). Variables observed were pH value, N-NH3 concentration, total volatile fatty acid (VFA), dry matter and organic matter digestibility. Data obtained was analysed using Analysis of Variance and any significant different further tested using Duncan Multiple Range Test. The results showed that there was no interaction between sources of diet and buffer. Supplementation of canola and flaxseed oils protected by calcium soap at level 6% did not affect pH value, dry matter digestibility, rumen protozoa and total bacteria. The use of different buffers affected pH value, dry matter digestibility, rumen protozoa and total bacteria. It’s concluded that the used of flaxseed oil or canola oil calcium soap did not alter rumen fermentation and McDougall buffer could improve in vitro fermentation activity compare to Kajikawa buffer. Keywords: biohydrogenation, calcium soap, canola oil, flaxseed oil, rumen fermentation

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