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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 20 Documents
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Search results for , issue "Vol 17, No 1 (2013): Annales Bogorienses" : 20 Documents clear
Isolation of Microorganisms and Its Application for Decolorization of Anthraquinone and Azo Dyes from Textile Wastewater Fathoni, Ahmad; Jeong, Soo Kyoung; Kim, Joong Kyun
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/74

Abstract

The treatments of textile wastewater by chemical and physical methods have some drawbacks such as economically feasible and high cost, lack of effective color reduction and formation of by-product. Conversely, biological processes have been proposed as a less expensive and less environmentally intrusive alternative. In the present study, decolorization of various dyes was studied using microorganisms isolated from textile/dye wastewater inoculated into culture medium supplemented with 0.01% of anthraquinone and azo dyes (Disperse Red 73, Lanasol Blue 3G Disperse Orange 30, Kemachrome Black T, and Mixture of dyes). The effect of medium composition was investigated by applying four different media (BSM, ME, Basal, and GYP). From 42 isolates, 4 isolates showed the potential for decolorizing structurally different anthraquinone and azo dyes employed in the industry. Based on the microscopic observation, the 3 isolates designated as OW1, RW8, ATSN-3 were identified as bacteria and 1 isolate designated as BSS was observed as fungus. BSS fungus isolate showed the highest ability to decolorize various reactive dyes including anthraquinone and azo dyes. BSS grew well in the ME medium, resulting in approximately 96.4% decolorization efficiency after 72 hours. The result indicates the potential for this isolate to be used in the biological treatment of textile industry.
Back Cover AB Vol 17 No 1 (2013) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.744 KB) | DOI: 10.1234/170

Abstract

Polyaromatic Hydrocarbon Degradation and Dioxygenase Gene Detection from Alteromonas alvinellae Bt05 Thontowi, Ahmad; Rahmani, Nanik; Yopi, Yopi
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/75

Abstract

Bt05 is marine bacterium which was isolated from the Jakarta Bay, Indonesia. The aim of this study was to characterize PAHs-degrading property, molecular identification by partial analysis of 16S rRNA gene and to partially analyze dioxygenase gene of Bt05 isolate. Our further study on this isolate revealed that it could degrade three PAHs (phenanthrene, dibenzothiophene, fluorene) between 60%–90% within 11 days at 100 ppm level. This finding indicated the potential of the isolate for bioremediation of PAHs. The isolate was identified as Alteromonas alvinellae by phylogenetic analysis of 16S rRNA gene sequence. Sequence analysis of the PCR product of PAH dioxygenase genes amplified using two primer set (iiDA and ppAH) of the isolate were identified 97% as naphthalene dioxygenase gene (phaAc) and 58% as 1,2-dioxygenase.
Editors Preface AB Vol 17 No 1 (2013) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1324.165 KB) | DOI: 10.1234/171

Abstract

Growth and Phycocyanin Productitivity of Spirulina fusiformis under Various Light Regimes Chrismadha, Tjandra; Waluya, Rizky Agus
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/71

Abstract

A blue green alga Spirulina fusiformis was grown under various light regimes in a laboratory scale experiment. A 500 watt halogen lamp was employed as the light source, while the light variation of 2,000 lux, 4,000 lux, 6,000 lux, 8,000 lux, and 10,000 lux was obtained by placing a series of 2 L experimental bottles at various distance. The growth medium used was modified Zarrouk medium with initial pH of 8.72, and room temperature was 28-30oC. After inoculation the alga was let to grow for 30 days, and observation on the biomass, chlorophyll, crude protein, and phycocyanin content were carried out every 10 days. The algal biomass was determined gravimetrically, the chlorophyll, phycocyanin, and protein content were measured using spectrophotometer after extraction in 90% aceton, pH 7.0 buffered water, and folin-phenol dye binding, respectively. The result shows a remarkable effect of light intensity to the algal biomass as well as the biochemical content. The specific growth rate increased from 0.08 doubling/day at light intensity 2,000 lux to 0.14 doubling/dat at 10,000 lux, which was equivalent to an increase in the biomass productivity of more than 3 times. The highest algal chlorophyll content was observed at light intensity between 4,000-8,000 lux, indicating the optimum light condition at that irradiance range. The protein content was consistently lower with light intensity, from 42.96-52.91% DW at 2,000 lux to 33.71-41.08 % at 10,000 lux. A consistent drop in the protein content also observed with the culture growth phase, from 39.82-52.91% DW in the early growth stage down to 33.71-42.96% at day 30. Light intensity in concomitance with the growth phase remarkably increase the algal phycocyanin content. In the early growth stage the phycocyanin content was ranged from 0.16% DW at 2,000 lux up to 1.229% DW at 10,000 lux, whereas at the end of the experiment the algal phycocyanin content were 1.04% DW and 2.436% DW, at 2,000 lux and 10,000 lux, respectively. It gave a consequence of more than 7 times higher phycocyanin productivity, which was from 0.09 mg/L/day at 2,000 lux to 0.62 mg/L/day at 10,000 lux. This result shows the importance of light factor in producing phycocyanin from the blue green alga Spirulina fusiformis.
Identification of Differentially Expressed cDNA in Cassava under Drought Stress Using cDNA-RAPD Approach Hartati, N Sri; Supatmi, Supatmi; Aryaningrum, Pramesti Dwi; Sudarmonowati, Enny
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/72

Abstract

Cassava is an important carbohydrate source that provides food security and alternative renewable energy development. This plant is naturally drought tolerant, but there is a wide variation within cassava genotypes in their ability to maintain high yield and starch production under drought stress. It has been reported that cassava clones with leaf retention or stay green trait can produce more total fresh biomass and high root dry matter compared to drought susceptible cultivars. The genetic, biochemical and molecular bases of stay green trait need to be understood to develop drought resistant cassava cultivars since water stress limits yield and starch production. Differential Display (DD)RT-PCR is a powerful technique for analyzing differences in gene expression. The method is based on the detection of the differentially expressed cDNAs from two or more samples. The main objective of this study was to identify differentially expressed cDNA in cassava under drought stress by employing a cDNA-RAPD approach. In this study, we used cassava genotype Ubi Kuning which was considered the most responsive to water insufficiency (45 days without watering). Leaf samples were collected from water stressed and well-watered plants at day 45. Among 11 random primers, OPB03 and OPH17 have identified differentially expressed cDNA in Ubi Kuning. Further characterization of these PCR products of expressed cDNA under drought stress may open possibility the development of cassava with improved drought resistance through genetic engineering and/or marker assisted selection (MAS).
Guide for Authors AB Vol 17 No 1 (2013) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.899 KB) | DOI: 10.1234/173

Abstract

Isolation of Endophytic Microbes from Gunung Halimun National Park, West Java, Indonesia and Bioassay Their Potency for Eradicating Microbial Crops Pathogen Sukara, Endang; Melliawati, Ruth
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/73

Abstract

Gunung Halimun National Park (GHNP) – West Java, Indonesia is the largest preserved primary forest in West Java. Diversity of plants and animals of this park have been studied intensively during the 15 years years. Diversity of endophytic microorganisms, however, has never been reported. Endophytic microbes are those who reside in the interspatial tissues of plants, have a capacity to produce array of secondary metabolites. This paper illustrates the occurrence of endophyte microbes in diverse flowering plants of GHNP. Total of 160 bacteria and 337 fungus were isolated from 86 plants species in the area. Out of that, 159 bacterial isolates and 320 fungus isolates survived in our collection and tested against four major microbial crops pathogen namely Xanthomonas campestris, Pseudomonas solanacearum, Colletotrichum glocosporoides and Fusarium oxysporum cubense. Plate Agar Test Assay method reveals that 51 among 159 bacterial isolates and 62 among 320 endophytic fungal isolates have an ability to inhibit the growth of microbial crop pathogens. Endophytic bacteria can inhibit more microbial crops pathogen but the inhibition ability is less compare to that of endophytic fungi. From our preliminary study clearly shows that endophytic microbes of GHNP should have potential value in developing biological control agent to combat microbial crop pathogens and eventually reduce the use of synthetic chemicals.
Front Cover AB Vol 17 No 1 (2013) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/169

Abstract

Editorial Boards AB Vol 17 No 1 (2013) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/172

Abstract

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