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ILMU KELAUTAN: Indonesian Journal of Marine Sciences

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Published by Universitas Diponegoro

ISSN : 08537291 EISSN : 24067598 DOI : -

Core Subject : Science,

Earth & Planetary Sciences

ILMU KELAUTAN: Indonesian Journal of Marine Sciences (IJMS) is dedicated to published highest quality of research papers and review on all aspects of marine biology, marine conservation, marine culture, marine geology and oceanography.

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Articles 7 Documents

Search results for , issue "Vol 21, No 4 (2016): Ilmu Kelautan" : 7 Documents clear

Effects of Microencapsulated Synbiotic Administration at Different Dosages against heavy co-infection of White Spot Disease (WSD) and Vibrio harveyi in Pacific White Shrimp (Litopenaeus vannamei) Yunarty Yunarty; Munti Yuhana; Widanarni Widanarni
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

White spot disease (WSD) is one of infectious disease in shrimp caused by white spot syndrome virus (WSSV). This study aimed to determine the dosage immunological effects and growth performances of microencapsulated synbiotic (Bacillus NP5 and mannan oligosaccharide) at different dosages on Pacific white shrimp. The microencapsulated synbiotic was administered as feed supplementation against the co-infection of WSSV and Vibrio harveyi. Synbiotic was encapsulated by spray drying method, further feed supplemented to Pacific white shrimp for 30 days at a dosages of 0.5% (A), 1% (B), 2% (C) and control treatments, i.e. without any microencapsulated synbiotic administration as positive control (D) and negative control (E). The challenge test was performed on day 30 after feeding supplementation, then the experimental shrimps were injected by WSSV intramuscularly at the infective dosage of 104 copies.-ml-1. Afterwards, 24 hours after WSSV injection the shrimps were immersed in water contained cells suspension of V. harveyi at the cells population dosage of 106 CFU-.ml-1. All synbiotic treatments showed better results with the values of Total Haemocyte Count (THC), Phenoloxidase (PO) and Respiratory Burst (RB), were higher (P<0.05) compared to positive control. The specific growth rates (SGR) of A, B and C showed higher than both controls of D and E. The feed conversion ratio (FCR) value of synbiotic treatments were lower (P<0.05) than both controls. However, the administration of microencapsulated synbiotic have not been able to prevent heavy impact of WSSV and V. harveyi co-infection due to lower SR and mortality pattern which continued to increase. Keywords: Synbiotic, Litopenaeus vannamei, WSSV, Vibrio harveyi, co-infection

Ecological Asessment In Semarang Coastal Area Based On Sediment Bioassay Approach Using Green Mussel Larvae Rachma Puspitasari; Triyoni Purbonegoro
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Rapid developments of industry and population growth have lead to ecological pressures on coastal areas. Semarang as capital of Central Java and port city, has an environment sensitivity which is important to be investigated. Sediment is an important part in aquatic environment because acts as sink or source of pollutant and could be used to assess coastal environment health. Three locations had been studied, i.e. Tanjung Mas Port, estuaries of Banjirkanal Barat and Banjirkanal Timur. These locations were compared to assess their health of sediment through elutriate sediment toxicity test. Eighteen grams of sediment from each locations were weighed and placed in 1 L Beaker glass. Eggs and sperm are taken from spawning of adult green mussel in laboratory. Thirty mL of sperm was added to the eggs and fertilized eggs were observed microscopically. Fertilized eggs were exposure to elutriate water-sediment from each location for 48 hour. Statistical analysis showed that there are significantly different abnormalities larvae percentage of sites 5, 8 and 13. The highest percentage of abnormality larvae occurred in Port Tanjung Mas, followed by Banjirkanal Timur and Banjirkanal Barat. Lead concentrations in sediments of coastal Semarang was ranged from 10.9 to 15.62 mg.kg-1, with an average of 13.69 mg.kg-1. Lead concentration was decreased form Port, Banjirkanal Timur and Banjirkanal, respectively. The abnormalities of green mussel larva may related to lead contamination. It could be concluded that sediment quality in Port area was very poor compare than other site. Keywords: bioassay, green mussel larvae, sediment, Semarang coastal area

Screening of Antibacterial MDR derived from Sponge Associated Fungus of Riung Water, Nusa Tenggara Timur Khoeruddin Wittriansyah; Agus Trianto; Sekar Widyaningsih; Ocky Karna Radjasa; Rudhi Pribadi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Marine sponge-associated fungi are the sources of bioactive compounds with various pharmacologicals potency. This study aimed to isolate the sponge-associated fungi as the producer of the MDR anti-bacterial compounds. The associated fungi were isolated from the sponges collected from Riung water, Nusa Tenggara Timur. Five of the best isolates were cultured on MEA to obtain the methanolic extract for further studies. The antagonistic test was conducted using overlay method towards the MDR Staphylococcus aureus and Escherichia coli. A total of 33 fungi were isolated from 19 sponge specimens. The antagonistic test showed that 19 isolates were active against both S. aureus and E. coli, and 13 of them were merely active against one of the bacteria. However, only five isolates have strong activity against one or both of the bacteria. The KN-15-3 had the strongest activity against S. aureus (18.75±0.777mm) and E. coli (15.10±0.141mm) at the concentration of 400 μg.disc-1 so it can be developed further as a source of drug candicate. Keywords: Fungi symbiont, Sponges, MDR Antibacterial, Staphylococcus aureus, Escherichia coli.

Identification of Carotenoids in Halimeda macroloba Reef Associated Bacteria Wiwik Astuti; Ocky Karna Radjasa; Ferry Fredy Karwur; Ferdy S. Rondonuwu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Several carotenoid pigments which are produced by sea microorganisms are shown to function as antimicrobe compounds and potential antioxidants. Coral reefs with high levels of diverse biota facilitate Halimeda as a primary component and producer to have associated bacteria that produce relatively unknown metabolic compounds. This research attempts to isolate bacteria that are associated with Halimeda macroloba, identify it, as well as analyze the pigment content produced. A yellow-orange bacteria colony is successfully isolated and given the temporary name MK_HM. This bacteria is in the shape of circular (oval) rods and are gram-variable bacteria. Based on the sequencing analytical results, Blast homology, as well as a phylogenetic analysis, it shows that the bacteria have a relationship with Exiguobacterium aestuarii TF-16 at 94%, so that it can be strongly predicted that the MK_HM strain is a new species. This pigment analysis, which is conducted on the Exiguobacterium sp. MK_HM bacteria extract, reveals that this strain produces carotenoid pigments of diadinochrome, semi-α-carotenone, dinoxanthin, and P457. Keywords: Exiguobacterium, carotenoid, diadinochrome, semi-α-carotenone, dinoxanthin, and P457.

Composition of Juvenile Corals on Different Morphotypes of Substrate at Karimunjawa Archipelago, Indonesia Munasik Munasik; Diah Permata Wijayanti; Retno Hartati; Rudi Pribadi; Yoko Nozawa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Patterns of coral recruitment are partially explained by fitness consequences of substrate selection, and may be affected by substrate morphology. This study examined juvenile coral assemblages on morphologically different dead coral substrate in shallow water reefs (4-5 m) at Karimunjawa Island, Java Sea (Indonesia). Surveys on juvenile corals were performed using 0.5×0.5 m transects on two different morphotypes of natural substrate; tabular dead corals (stable) and branching dead corals (unstable). Results showed that the morphological characteristics of dead coral substrate had a significant influence on the generic composition of juvenile corals. Coral recruits on tabular dead coral substrate (stable) which composed micro-cervices was more diverse than on branching dead coral substrate (unstable). Juvenile corals of the genus Acropora were dominant on (exposed microhabitats of) tabular dead coral substrate, while those of the genus Fungia and Montipora were dominant on (cryptic microhabitats of) branching dead coral substrate. These results suggest that Acroporid juveniles can besettled to the various morphotypes of the substrate, thus the distribution pattern of juvenile corals in shallow reef Karimunjawa may correlate with the distribution pattern of natural substrates.Keywords: juvenile, microhabitat, dead coral, substrate Karimunjawa Archipelago

Recruitment Status of Coral Reefs (Scleractinian) after Earthquake and Tsunami in North Pagai Island of Mentawai Islands Regency Suparno Pranoto; Arlius Arlius
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Recruitment of coral is marked by the appearance of reef colonies that are still juvenile. Coral recruitment data after the earthquake and tsunami are very few either in Indonesia or in other part of the world. The purpose of this study is to analyze the level of recruitment and to analyze recruitment diversity of coral reef Scleractinian in the waters affected by tsunami (west coast) and not affected by tsunami (east coast) of North Pagai Island. The observation on coral recruitment used benthic quadrate sampling method with a size of 1x1m2 and the diameter of colony taken measured between 0.5–10 cm. The result of the study shows that the average of recruitment density at the west coast (affected by tsunami) ranged between 0.78–3.67 colonies.m-2 and at the east coast (not affected by tsunami) ranged between 5.11–11.67 colonies.m-2. Coral recruitment level of the east coast is within the category of medium to very high while coral recruitment level of the west coast is in very low to low category. Diversity index (H') of east coast and west coast ranged between 1.55–2.54 with medium category, evenness index (E) of east coast and west coast ranged between 0.87–0.97 with stable category, and dominance index (C) with values ranging from 0.10–0.13 with low category.. Types of coral Porites cylindrica is growing rapidly and has the highest level of recruitment after the tsunami. Keywords: recruitment reef, tsunami, coral, North Pagai Island

Angular Range Analysis (ARA) and K-Means Clustering of Multibeam Echosounder Data for Determining Sediment Type Fahrulian Fahrulian; Henry M Manik; Indra Jaya; Udrekh Udrekh
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Backscatter value was a key to determine seabed characteristic. Level of intensity or backscatter informed through seabed type. One approach was use to analyze seabed type based on the value of backscatter was Angular Range Analysis (ARA). ARA utilize influence of angle backscattering intensities. The aim of this research was to determine value of backscatter from the bottom and used to initial prediction of seabed. Extraction processes of raw data obtained by acoustic signal processing techniques. Analysis of backscatter data was conducted by using K-means method to look the proximity of the centroid backscatter value against other values. Backscatter intensity from this sites ranged from -41,93 dB to -27 dB. The range value divided into three major classes based on Wenworth scale classification. Substrate type in the study site consists of sand, silt, and clay. Grain size diameter of each type include: sand 0.122713 mm (phi = 3.02), silt 0.018171 mm (phi = 5.78) and clay 0.002690 mm (phi= 8.53). Based on this result, signal processing multibeam echosounder able to classify seabed backscatter values to determine the seabed type Keywords: Angular Range Analysis, multibeam echosounder, K-Means

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