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Indonesian Aquaculture Journal

iaj
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Published by Kementerian Kelautan dan Perikanan RI

ISSN : 02150883 EISSN : 25026577 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.

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Articles 10 Documents

Search results for , issue "Vol 8, No 1 (2013): (June 2013)" : 10 Documents clear

PCR-DGGE METHOD FOR MICROFLORA ALTERATION IN SHRIMP DIGESTIVE ORGAN FOLLOWING Lactobacillus ADMINISTRATION Nunak Nafiqoh; Poh-Shing Chang; Yu-Chi Wang
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Recently, bacteria as probiotic usage in aquaculture are deeply investigated. Probiotic application in aquaculture leads to alteration in bacteria community within environment and inside digestive system of the host. However, the evidence of treated bacteria presence is very limit. This study was aimed to reveal the effectiveness of PCR-DGGE method to bring some evidence of the applied probiotic existence within aquatic organism. Two species of Lactobacillus were applied in this experiment. Litopenaeus vannamei was used as host for applied bacteria, L. vannamei was reared using natural sea water in the 45 cm x 15 cm x 25 cm aerated glass tank. Twice daily of enriched Artemia were gived as nutrition during experiment. The result showed that Lactobacillus represent higher in treatment group compare with control after 20 days treatment. In the other hand there was no different of bacteria number between two treated Lactobacillus. PCR-DGGE is a rapid and reliable method for bacteria detection within aquatic organism.

HERITABILITY FOR GROWTH RELATED TRAITS IN GIANT FRESHWATER PRAWN (Macrobrachium rosenbergii) AT VARIOUS DEVELOPMENTAL STAGES AND CULTURE CONDITIONS ESTIMATED BY INTRACLASS CORRELATION Imron Imron; Rommy Suprapto
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Heritability estimates of commercially important traits are of important in order to seek the best strategy of selective breeding program to be implemented. A study aimed at estimating the magnitude of this parameter for growth related traits, expressed in wet weight (WW), total length (TL), and standard length (SL), has been carried out in giant freshwater prawn (GFP). Particular emphasis was given to investigate the effect of ages and culture conditions on the magnitude of the heritability estimates. Nineteen full-sib families were established through individual pair mating. The families, namely groups of offsprings derived from each mating pair were raised through three stages of rearing activities: first-stage nursery (40 days), second-stage nursery (70 days), and grow-out rearing (130 days). Heritability for growth at each stage was estimated through the method of full-sib analysis or intraclass correlation. Components of variance used to produce the heritability estimates were obtained through the method of analysis of variance. Results showed that heritability estimates varied with both ages and culture conditions. The heritability estimates (± standard errors) at 40 days for WW (0.69±0.151), TL (0.64±0.148), and SL (0.70±0.144) were higher than those observed at 70 days (WW = 0.24±0.15; TL = 0.22±0.15; and SL = 0.20±0.14) and 130 days (WW = 0.24±0.058; TL = 0.22±0.05; and SL = 0.20±0.60). A similar pattern was found with respect to the culture conditions. The estimates found in grow-out at lower stocking density (5 individual/ m2) days (WW = 0.24±0.058; TL = 0.22±0.05; and SL = 0.20±0.60) were higher than those observed at grow out at higher stocking density (20 individuals/m2) days (WW = 0.12±0.058; TL = 007±0.05; and SL = 0.14 ± 0.60). The possible causes of the observed patterns and implications that these findings may have on the breeding program of GFP are discussed.

NEW MEGALOCYTIVIRUS INFECTED TO THE CULTURED FRESH WATER GIANT GOURAMI, Osphronemus gouramy Lac. IN INDONESIA Isti Koesharyani; Lila Gardenia
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Giant gourami (Osphronemus gouramy Lac.) is one of freshwater fish having high economic value and very popular in Indonesian food restaurant. This fish have been cultured throughout Indonesia. Since two years ago, cultured fish in ponds infected by unknown pathogenic agent and may cause diseased fish and mass mortality. Mass mortality of pond cultured giant gourami occurred in West Java, Central Java, and Bali-Indonesia. The symptoms of diseased fish are the body color becomes darker, resting on the water surface, and internal organs of fish characterized by enlargement of spleen. In the present study diseased fish samples were diagnose by Polymerase Chain Reaction (PCR) techniques using specific primers of red sea bream iridovirus (RSIV) and histopathologically analysis to determine the causative agent of mortality. Based on PCR analysis, show that all fish sampled were positive infected by megalocytivirus and infected fish mostly died. This is a new and first time reported virus infection in cultured giant gourami in Indonesia and it is proposed to be named as giant gourami iridovirus (GGIV).

INFLUENCE OF DIETARY PHYTIC ACID AND PHYTASE ON GROWTH, DIGESTIBILITY, AND VERTEBRAL PHOSPHORUS OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus Asda Laining
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Triplicate groups of juvenile Japanese flounder (0.56 g) were fed with six experimental diets to examine the effects of phytic acid, with or without phytase on growth performances, nutrient digestibility, and vertebral phosphorus (P) content. Diet without both phytic acid (PA) and phytase supplementation was used as control. One diet was added with 10 g PA/kg without phytase supplement. Four diets were formulated to contain two levels of phytase (1,000 FTU or 2,000 FTU phytase/kg diet) combined with 2 levels of PA (10 and 20 g/kg diet). All diets were added with 10 g/kg in-organic P to meet flounder requirement. After 50 days culture, fish fed 10PA/2,000P grew significantly (P<0.05) higher than other groups, but did not differ from fish fed 10PA/1,000P. In contrast, fish fed 10PA/0P had the lowest growth but was not different from control diet (0PA/0P). Addition of either PA or combined with phytase had no significant (P>0.05) effects on feed intake and FCR. However, fish fed 10 g PA/kg combined with phytase had significant (P<0.05) higher whole body lipid, ash, and P than other groups. Moreover, P digestibility and vertebral P content were significantly increased by dietary phytase. This finding suggested that dietary phytase had potential to enhance the growth and nutrient utilization in juvenile Japanese flounder fed diet containing phytic acid. Specifically, inclusion of 2,000 FTU phytase/kg diet gave better performances when diet containing PA at level of 10 g/kg diet.

THE STUDY ON MANGROVE LITTERS AS A SOURCE OF NUTRIENTS FOR BLANAKAN MANGROVE POND, SUBANG, WEST JAVA Joni Haryadi; Adi Basukriadi; Muhadiono Muhadiono
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Mangrove litters as a source of nutrients for sylvofishery pond (TS), sedimented pond (TT), and conservation pond (TP) was studied at Blanakan mangrove pond during April until June 2008. The variables of study were litter production, litter decomposition, and dissolved nutrients (ammonium, nitrite, nitrate, and phosphate). The obtained data were analyzed using F test and continued using Least Significant Different (LSD). The results showed that litter production at TS, TT, and TP were 19.55±4.34 ton/ha/year, 15.90±1.98 ton/ha/year, and 21.67±1.89 ton/ha/year respectively. The decomposition rate at TS, TT, and TP was 0.051±0.038, 0.051±0.018, and 0.081±0.041, respectively. Mangrove litters were potentially as a source of dissolved nutrients at Blanakan mangrove pond. Increasing both litter production and decomposition rate could increase ammonium, nitrite, and phosphate. Therefore, mangrove litters were play role for determined the fertility at Blanakan mangrove pond.

INTERACTION BETWEEN DIETARY MINERAL AND PHYTASE ON BIOLOGICAL PERFORMANCES OF JAPANESE FLOUNDER, Paralichthys olivaceus. PART I. GROWTH, FEED INTAKE, AND WHOLE BODY MINERAL CONTENT Asda Laining; Lidemen Lidemen; Manabu Ishikawa
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

In order to determine the effect of dietary calcium (Ca), inorganic phosphorus (IP), and phytase (P) supplementation in marine fish, a 2 x 2 x 2 factorial design was arranged to observe the interrelationship between dietary Ca and IP with the presence of dietary phytase. Two levels of dietary Ca at 0% and 0.2% combined with either 0% or 0.25% of dietary IP and either with 0 and 2,000 FTU phytase/kg diet, respectively to formulate eight experimental diets. SPI-based diet was used as basal diet and the sources of Ca, IP, and phytase were similar to those used in the previous experiment. Juvenile Japanese flounder with initial body weight around 0.6 g was fed the test diets. After 30 days of feeding trial, the results showed that both dietary IP and phytase, but not dietary Ca significantly enhanced the growth and feed intake. The highest growth was achieved in fish fed a diet containing the Ca, IP, and phytase supplement among groups. Fish fed diet without the three dietary supplements had the lowest SGR and did not significantly improve by supplementing dietary Ca. Feed intake (FI) and was significantly influenced by dietary IP and phytase, not dietary Ca while feed conversion ratio (FCR) was significantly affected by all dietary treatments. Interaction effect was detected between dietary Ca and IP, between dietary IP and P on FCR. Total phosphorus content in whole body was significantly increased by supplementing all dietary treatments which was highest in fish fed 0.25 IP/0.2 Ca/P. Significant interaction was observed between dietary IP and P on this parameter. Whole body Ca content was significantly improved by either dietary IP or Ca and not dietary P. As conclusion even without inorganic Ca supplement, dietary IP at level of 0.25% or 2,000 FTU phytase/kg diet could enhance growth and FI of fish as well as whole body phosphorus content of juvenile Japanese flounder when diet basal contained organic Ca around 1.2%.

ELECTRON MICROSCOPIC ANALYSIS OF ENLARGED CELLS DERIVED FROM RED SEA BREAM IRIDOVIRUS (RSIV)-INFECTED CULTURED GRUNT FIN (GF) CELLS Ketut Mahardika
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Red sea bream iridovirus (RSIV) has been known to induce cellular enlargement as cytophatic effect (CPE) in cultured cell line. In the present study, grunt fin (GF) cells were treated with RSIV. After advanced of CPE, the cellular enlargement were harvested, processed and analysis under electron microscopy. Electron microscopy revealed inclusion body bearing cells (IBCs), and enlarged and rounded cells allowing virus propagation within the intracytoplasmic virus assembly site (VAS). Most were enlarged cells. These enlarged cells were divided into three cells types. Enlarged cells of the type I, which contained many mature virions were numerous in the number rather than enlarged cells of type II which contained many immature viral particles and enlarged cells of type III which allowed assembly of a few virions. These results determined that basic ultrastructure feature of RSIV infected GF cells is formation of IBCs and cells containing a few, many mature and immature viral particles within VAS.

THE OPTIMAL RATIO OF NILE TILAPIA (Oreochromis niloticus) AND COMMON CARP (Cyprinus carpio) FOR IMPROVING PRODUCTIVITY ON DEEP WATER POND Imam Taufik; Sutrisno Sutrisno; Eri Setiadi
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Pond productivity can be increased by applied polyculture system in the deep pond. The purpose of this experiment is to examine the optimal ratio between nile tilapia and common carp, in order to increase the productivity. Nine concrete tanks (15 m2) with water depth of 2.2 m and were completed by water inlet, water outlet, and aeration. Both of nile tilapia and common carp with size ranging of 5-8 cm in total length were used. Stock density was 150 ind./m2. The difference ratio of both fish tilapia and carp of fish stocked as a treatment. The fish ratio this experiment were as followed: A) 100%; B) 80%:20%; C) 60%:40%. Fish fed by pellet until at ad libitum. The duration of experiment was 100 days. Parameters such as survival, growth, and productivity were observed every ten days during the experiment period. Water quality parameters were also periodically observed. The results showed that survival of nile tilapia among the treatments were not significantly different (P>0.05) where survival of common carp at B treatment was better than C treatment (P<0.05). The highest of growth of absolute weight (94.86±2.85 g) and total length (14.71±1 cm) of nile tilapia at B treatment was found (P<0.05) where the best of growth of absolute weight (106.52±10.47 g) and total length (11.57±1.78 cm) of common carp was also found at B treatment (P<0.05). Biomass productivity at B treatment was the highest compared with A treatment (P<0.05). Combination between polyculture and the deep water pond technology could increase productivity. The polyculture system and the deep water pond technology would be able to keep constant water quality within in the threshold accordance with the regulation for fish culture.

COLOUR QUALITY ENHANCED ON GOLDFISH JUVENILES THROUGH SHRIMP HEAD MEAL ENRICHED IN FEED I Wayan Subamia; Nina Meilisza; Asep Permana
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Goldfish (Carassius sp.) is the ornamental fish which has been managed its culture by fish farmers. The objective of the research is to improve the colour quality of goldfish juveniles through enrichment of feed using shrimp head meal as source of carotenoid. The research was conducted at The Research and Development Institute for Ornamental Fish Culture, Depok, using completely randomized design with four treatments of shrimp head meal ratio, 0% (control), 5%, 10%, and 15% in feed, the feed formulation contained isoprotein (30%), and isolipid (15%) and three replications of each treatment. Ten juveniles of Carassius sp. with body weight of (1.08±0.02 g) stocked in aquarium with water volumes of 20 L. The growth and colour quality in qualitative standard using TCF (Toca Colour Finder) were examined, while total carotenoid content on feed were measured by spectrophotometry method. The colour quality of goldfish were also measured at three of fins those were dorsal, ventral, and caudal. The result showed that no significant different on growth and survival rates among the treatments. Based on the present research, the optimal colour improvement to goldfish was by addition of 10% shrimp head meal in feed.

SPECIFICITY OF Haemolysin AND Gyrase GENE MARKER FOR RAPID DETECTION OF VIBRIOSIS ON PENAEID SHRIMP Ince Ayu Khairana Kadriah; Sukenda Sukenda; Munti Yuhana; Endang Susianingsih; Muharijadi Atmomarsono
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Pathogenic Vibrio spp. have been implicated as being one of the major causes of the disease problems in penaeid shrimp culture. For many pathogens, the outcome of the interaction between host and bacterium is strongly affected by the bacterial population size. Efforts for rapid molecular detection is to isolate specific genes which was owned by the pathogenic luminescent Vibrio and used it as the marker molecular in the rapid diagnosis of the disease. Haemolysin and gyrase gene known as one of the specific genes possessed pathogenic bacteria including Vibrio. The objectives of this study were to determine the specificity of designed specific primer from local isolate. Specificity test was performed for haemolysin primer (IAVhF1 and IAVhR1), gyrase primer (IAGyrF1 and IAGyr2) and commercial detection kit. The specificity test comparing the PCR result of pathogenic Vibrio DNA template as positive control, and DNA template from non pathogenic luminescent Vibrio, as well as pathogenic non Vibrios. The result showed that at annealing temperature of 60°C, haemolysin primer was more specific in detecting pathogenic Vibrio for penaeid shrimp than that of gyrase primer and commercial detection kit.

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Home Page

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Abstract

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Contact Name
Ofan Bosman

Contact Email
Ofan Bosman

Phone
-

Journal Mail Official
iaj.puslitbangkan@gmail.com

Editorial Address
-

Location
Kota adm. jakarta pusat,

Dki jakarta

INDONESIA