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Indonesian Aquaculture Journal

iaj
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Published by Kementerian Kelautan dan Perikanan RI

ISSN : 02150883 EISSN : 25026577 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.

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Articles 10 Documents

Search results for , issue "Vol 9, No 2 (2014): (December 2014)" : 10 Documents clear

ALTERNATE USE OF DIFFERENT RICA PROBIOTIC BACTERIA ON SURVIVAL RATE AND PRODUCTION OF CULTURED TIGER SHRIMP IN SEMI-INTENSIVE PONDS Muharijadi Atmomarsono; Nurbaya Nurbaya
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Tiger shrimp diseases have occured in Indonesian shrimp ponds for more than two decades. In order to overcome this problem, five types of the RICA probiotic bacteria were tested in nine 250 m2 semi-intensive ponds of the Research and Development Institute for Coastal Aquaculture Marana Station. In the present study three different alternate use of the RICA probiotics were tested for tiger shrimp culture incompletely randomized design experiment. There were three treatments here namely: A) alternate use of probiotic bacteria RICA-1, RICA-2, RICA-3; B) alternate use of probiotic bacteria RICA-4, RICA-5, RICA-3, and C) control (without probiotic bacteria); each treatment were applied in three replications. The results showed that survival rate and production of tiger shrimp in treatment A (55.8% and 14.9 kg/pond) and B (52.7% and 16.7 kg/pond) were significantly better (P<0.05) than those of in control ponds (37.4% and 10.9 kg/pond). However, survival rate and production of tiger shrimp between treatment A and B were not significantly different (P>0.05). The average total bacteria population in the cultured water media of treatment A (4.32 x 104 cfu/mL) and B (5.18 x 104 cfu/mL) were relatively higher than control (1.46 x 104 cfu/mL). However the percentage ratio of Vibrio spp. and total bacteria population in the cultured water media of treatment A and B were relatively lower than control. The lower survival rate and production of tiger shrimp in control (C) compared to probiotic treatments (A and B) were not just affected by the increase of Vibrio spp. ratio, but also affected by the increase of total organic matter and nitrite concentrations in the control ponds that were relatively higher than treatment A and B.

THE ROLE OF MACROBENTHIC COMMUNITIES AS AN INDICATOR FOR THE FERTILITY OF MANGROVE POND: CASE STUDY AT BLANAKAN, SUBANG, WEST JAVA Joni Haryadi; Hadiyanto Hadiyanto; Mufti Petala Patria
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Macrobenthic in mangrove ecosystems plays an important role as removal particles from the water column to the sediments. Ecology indexes of macrobenthic communities were used in this study as an indicator for the fertility of mangrove pond ecosystems. The study was carried out at mangrove pond in Blanakan, West Java. Data was collected from four sampling sites: opened pond (TB), sylvofishery pond (TS), sedimented pond (TT), and conservation pond (TP). Some ecological indexes such as Margalef’s Index (R), Shannon-Wiener Index (H’), Pielou Evenness Index (E), and Simpson Dominance Index (D) were used to analyze macrobenthic communities. The results showed that infaunal macrobenthic communities were stable at TP with R: 1.91±0.42, H’: 1.59±0.30, E: 0.74±0.14, and D: 0.29±0.12. They were unstable at TB with R: 1.63±0.80, H’: 1.36±0.32, E: 0.74±0.06, and D: 0.35±0.07. The macrobenthic communities can be used as indicator for the fertility of mangrove pond in Blanakan, West Java.

MICROBIAL COMMUNITY OF BLACK BAND DISEASE ON INFECTION, HEALTHY, AND DEAD PART OF SCLERACTINIAN Montipora sp. COLONY AT SERIBU ISLANDS, INDONESIA Ofri Johan; Dietriech Geoffrey Bengen; Neviaty Putri Zamani; Suharsono Suharsono; David Smith; Angela Mariana Lusiastuti; Michael Sweet
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

It is crucial to understand the microbial community associated with the host when attempting to discern the pathogen responsible for disease outbreaks in scleractinian corals. This study determines changes in the bacterial community associated with Montipora sp. in response to black band disease in Indonesian waters. Healthy, diseased, and dead Montipora sp. (n = 3 for each sample type per location) were collected from three different locations (Pari Island, Pramuka Island, and Peteloran Island). DGGE (Denaturing Gradient Gel Electrophoresis) was carried out to identify the bacterial community associated with each sample type and histological analysis was conducted to identify pathogens associated with specific tissues. Various Desulfovibrio species were found as novelty to be associated with infection samples, including Desulfovibrio desulfuricans, Desulfovibrio magneticus, and Desulfovibrio gigas, Bacillus benzoevorans, Bacillus farraginis in genus which previously associated with pathogenicity in corals. Various bacterial species associated with uninfected corals were lost in diseased and dead samples. Unlike healthy samples, coral tissues such as the epidermis, endodermis, zooxanthellae were not present on dead samples under histological observation. Liberated zooxanthellae and cyanobacteria were found in black band diseased Montipora sp. samples.

THE USE OF SEAWORM MEAL IN MATURATION DIET AS PARTIAL SUBSTITUTION OF FRESH DIET FOR POND REARED TIGER SHRIMP BROODSTOCK Asda Laining; Usman Usman; Rachman Syah
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The purpose of this experiment was to evaluate the effects of using seaworm meal in artificial diet as partial substitution of freshfeed for maturation of tiger shrimp. This experiment started by growing-out tiger shrimp with initial weight around 60 g for four months until reaching maturation phase where shrimp weight were over 90 g for female. Tiger shrimp was selected and stocked into 10 ton concrete tank with stocking density of 50 shrimps with ratio of female : male of 1:1. Dietary treatments were different levels of seaworm meal at 0% (SW0), 10% (SW10) and 20% (SW20). SW0 was positive control without seaworm meal but breeder was fed with frozen seaworm. Test diets were fed as a combination of 60% test pellet and 40% fresh feed. Artificial insemination was carried out for all females before ablation to obtain fertile eggs. Results showed that after ablation, number of female matured was highest in group fed SW10 (13 breeders) and the lowest in female fed control group (7 breeders). Number of female spawned was also highest in female fed SW10 and the lowest was in positive control. Fecundity was very low in all treatments ranged from 12,000-79,700 eggs/spawn. Even though female bearing spermatophore through insemination, number of spawning hatched was very low, only three spawned in each of SW0 and SW10 and two spawned in SW20. Based on number of breeders matured and spawning rate, breeder fed with SW10 gave better performance than other two diets. Technique of artificial insemination needs to be improved to increase the number of fertile eggs.

FREQUENT MONITORING OF WATER TEMPERATURE IN PEGAMETAN BAY, BALI: A PRELIMINARY ASSESSMENT TOWARDS MANAGEMENT OF MARINE AQUACULTURE DEVELOPMENT I Nyoman Radiarta; Erlania Erlania; Ketut Sugama; Hirmawan Tirta Yudha; Masaaki Wada
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Aquaculture currently share for nearly half of the world’s food fish consumption, and continue to be the fastest-growing animal food producing sector. The viability of aquaculture operation has greatly been affected by the characteristic of marine environment. Inventory and monitoring of marine environment are necessary and can be done through information technology implementation. Frequent monitoring of water temperature, for almost one year observation, at four aquaculture sites in Pegametan Bay and Research and Development Institute for Mariculture was investigated. Water temperature data were obtained by using logger and buoy systems. These data were contrasted against marine fish mortality. On the other hand, the suitability of species requirements with the thermal conditions was evaluated by comparing temperature range to the optimum and lethal temperature information available on marine fish species of aquaculture interest. This research could be beneficial for enhancing productivity of marine aquaculture operation in terms of possible impact of climate change. It was also possible to find the ideal temperature range for culturing fish species, taking into account the variability associated with large-scale phenomena.

DETECTION, TRANSMISSION, AND EXPRESSION cDNA GROWTH HORMONE GENE (PhGH) OF STRIPPED CATFISH IN F-1 TRANSGENIC AFRICAN CATFISH Huria Marnis; Bambang Iswanto; Rommy Suprapto; Imron Imron; Raden Roro Sri Pudji Sinarni Dewi
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

In previous study, the fast growth transgenic founder of African catfish was produced harboring a growth hormone (GH) gene construct containing a stripped catfish growth hormone (PhGH) cDNA. This study was conducted to investigate transgene (PhGH) transmission and expression in F-1 transgenic African catfish. The transgenic founders (female) were crossed with non-transgenic (male) to produce heterozygous F-1 progeny. PhGH gene was detected in the embryo, larvae, and seed of the transgenic F-1 using PCR method. Expression levels of transgene in embryo and larvae were analyzed using real-time quantitative PCR (qPCR) method. The transgene was detected in embryo, larvae and seed of F-1 transgenic African catfish. Founder could transmit PhGH gene to transgenic F-1 lines in ranged 36% to 48%. Expression level of Phgh gene in embryo was higher than that of the larvae; whereas in the embryo was 1.5 x 105 - 5.2 x 105 copies or 0.49-9.82 fold, while in the larvae was 1.1 x 105 - 2.5 x 105copies or 0.19-5.80 fold.

THE EFFECT OF PROBIOTIC ON IMMUNITY IMPROVEMENT IN THE FRY AND SPAWNER PRODUCTION OF PACIFIC WHITE SHRIMP Litopenaeus vannamei Haryanti Haryanti; Ahmad Muzaki; Sari Budi Moria Sembiring; Fahrudin Fahrudin; I Gusti Ngurah Permana; Ida Komang Wardana
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

In order to improve immune system of Pacific white shrimp L. vannamei from any kinds of diseases, various probiotic agents were tested such as Bacillus sp. BC, Alteromonas sp. BY-9 and mixed of both. The probiotics were cultured and added directly to the larval rearing tank (106 cfu/mL equal to 0.5 liter/m3) up to PL-12. In cultured shrimp spawners, probiotic was mixed with feed coated with chitosan, and without adding probiotic as control. In the present experiment, shrimp fry and spawners were reared in tank capacity of 5 m3 and 8 m3. Immunity response was analyzed by quantitative value of the immunity related gene expression with RTqPCR. The results showed that survival rate of shrimp fry cultured with mixed probiotic was 63.16% (PL-8) and 53.46% (PL-12), while control 13.35% (PL-8) and 12.48% (PL-12) respectively. The number of shrimp hemocytes starting from 420 to 530.5 x 104 cells/mL and after challenged with WSSV (for 72 hrs exposure) between two treatments of Alteromonas sp. BY-9 and Bacillus cereus BC was not significantly different P>0.05 (402.5 and 432.5 x 104 cells/mL), while in mixed probiotic and control treatments were 391.75 and 229.0 x 104 cells/mL, respectively. The analysis of immunity gene expression revealed that the use of probiotic from larval stages up to spawner has been proved in increasing immunity responses quantified from ProPO activating system (prophenoloxidase/proPO) as much as 1.6-2.0 fold, clotting system (transglutaminase, clotting protein) of 1.5-2 fold, Antimicrobial Peptide System (anti-LPS factor, crustin, lysozyme, penaiedin2) of 1.5-2.8 fold, and Antioxidant defense mechanism (superoxidase dismutase/SOD, glutathione peroxidase/GPx) of 1.8-2.0 fold compared to control.

FLUCTUATING ASYMMETRY REFLECT THE GROWTH OF HYBRID GROUPER Epinephelus fuscoguttatus AND Epinephelus polyphekadion Ketut Sugama; Ahmad Muzaki; I Gusti Ngurah Permana; Haryanti Haryanti
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Fluctuating asymmetry has been widely used as a measure of developmental stability and as an indicator of individual fish growth. The present study compared fluctuating asymmetry in three bilateral meristic traits of F-1 hybrid between female Epinephelus fuscoguttatus and male Epinephelus polyphekadion and two F-1 pure parental progenies. The fishes were reared by communal and separate tank systems. Hybrids were confirmed by allozymes electrophoresis. After three months of rearing, the F-1 hybrids fish grew faster 45.9% and 66.6% compare to the F-1 pure parental progenies of E. fuscoguttatus and E. polyphekadion (P<005) respectively. Development stability as measured by fluctuating asymmetry was lower in hybrid (20%-40%) than in the pure parental progenies (53%-80%). Among four enzyme loci examined, only Pgm locus showed variants and at that locus all hybrids fish were heterozygous. Heterozygous hybrids showed lower fluctuating asymmetry and related to higher developmental stability. The higher developmental stability positively reflected a faster growth rate. Thus this finding can be used to explain the reason that why hybrids groupers appeared grew faster than parental species.

FINE STRUCTURAL ANALYSIS OF UNPRODUCTIVE AND LOWPRODUCTIVE CELLS RESULTING ON CULTURED RSIV (IN VITRO) Ketut Mahardika; Teruo Miyazaki
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Red seabream iridovirus (RSIV) has been known to induce enlarged cells and inclusion body bearing cells (IBCs) allowing virus particles to propagate within viral assembly site (VAS) in the cell cytoplasm. The aim of this study was to evaluate fine structural analysis of unproductive and low-productive cells resulting on RSIV-infected cultured grunt fin (GF) cells. GF cells were treated with semi purified RSIV, and incubated for 6-14 days post cultured. The cellular enlargement were harvested, processed, and analysis under electron microscopy. Electron microscopy revealed four types of cells that were productive enlarged cells and productive IBCs which were allowing propagation of virus particles within its cytoplasm, and unproductive enlarged cells and IBCs without virus particles. Most of them were unproductive enlarged cells (80,71%-98,20%). Unproductive enlarged cell had a nucleus with enlarged cytoplasm without production of VAS with virus propagation. While, unproductive IBC had an inclusion body that was delimited from the host-cell cytoplasm by a thin membranous boundary, and was developed without virus propagation. On the other hand, lowproductive enlarged cells and IBCs contained a few number of virus particles or tubule-like structures. Therefore, the number of low-productive enlarged cells and IBCs were only a few (about 0%-14% from a total of percent productive enlarged cells and IBCs), these cells were classified into types of productive enlarged cells and IBCs. These results sugested that the unproductive and low-productive enlarged cells and IBCs were the results of RSIV-infected GF cells which failed to produce virus particles due to incapacity of RSIV virus it self and or the ability of GF cells to inhibit virus multiplication within VAS.

THE EFFECT OF MARINE SPONGE Aaptos aaptos EXTRACT IN VIBRIOSIS TREATMENT OF BLACK TIGER SHRIMP Penaeus monodon LARVAE Rosmiati Rosmiati; Najiah Musa; Habsah Mohammad; Tengku Sifzizul Tengku Muhammad; Andi Parenrengi; Wahyuni Wahyuni
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Black tiger shrimp Penaeus monodon post larvae were challenged with Vibrio harveyi and butanol extract of selected marine sponge Aaptos aaptos to determine its antibacterial bioactive potential in vibriosis treatment. Based on the preliminary toxicity study, the A. aaptos butanol extract with concentrations of 31.25, 62.5, and 125 mg/L were selected in the study. Black tiger shrimp post larvae were challenged with V. harveyi at 107 cfu/mL and immersed A. aaptos butanol extract with the concentration of 125 mg/L showed significantly in (P<0.05) decrease mortality of the post larvae treated. Besides at this concentration, V. harveyi population in the rearing water and the post larvae treated decreased compared to control (untreated post larvae). Histological observation indicated that there was no changing on hepatopancreas of the black tiger shrimp post larvae. Based on this result, it is suggested that the butanol extract of A. aaptos is a potential bioactive compounds source in the treatment of vibriosis which may replaced the current antibiotics application.

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Contact Name
Ofan Bosman

Contact Email
Ofan Bosman

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-

Journal Mail Official
iaj.puslitbangkan@gmail.com

Editorial Address
-

Location
Kota adm. jakarta pusat,

Dki jakarta

INDONESIA