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Medicra (Journal of Medical Laboratory Science/Technology)
Published by Universitas Muhammadiyah Sidoarjo
ISSN : 25807730     EISSN : 25807730     DOI : https://doi.org/10.21070/medicra
Core Subject : Health,
Health Professions Public Health
Focus : to facilitate scholar, researchers, and lecturers for publishing the original articles of review articles. Scope : Medicra publishes research articles in the field of “medical laboratory (science/technology)” with the following scope: Clinic Chemical Hematology Microbiology Parasitology Immunology Food and beverage analysis Chemical Molecular Diagnostics Toxicology Cytology Histology Epidemiology Laboratory Management Laboratory Quality Control
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 89 Documents
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Efficacy of Aloe vera Gel on the Excision Wound Healing in Sprague dawley Rats Bagus Uda Palgunadi; Asih Rahayu; Yos Adi Prakoso
Medicra (Journal of Medical Laboratory Science/Technology) Vol 4 No 1 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i1.1432

Abstract

Wound is a pathological processes in all living things. Wound includes the overlapping mechanisms. The failure in wound healing causes infection. This study aimed to analyze the efficacy of Aloe vera gel in excisionwound on the rat’s back. This study used 18 male, Sprague Dawley rats, and they were induced excision wound on the back. The rats were separated into 3 groups, K1 = control, K2 = treated with betadin gel, K3 = treated with Aloe vera gel. The therapy was given twice a day for 7 days. The wound measurement was observed in day 3 and 7. The data were analyzed using SPSS. The result showed that Aloe vera gel potentially promote wound healing through the decrease microscopical condition of wound, re-epithelialization, and deposition of collagen better than in group K1 and K2 (p≤0.05). In conclusion, Aloe vera gel promote excision wound healing in Sprague Dawley rats.
The Effect Of Using Personal Protection Equipment (PPE), Mileage, And Smoking Habits On Hair Lead (Pb) Levels Devyana Dyah Wulandari; Wardah Rohmah; Ersalina Nidianti; Andreas Putro Ragil Santoso; Ary Andini
Medicra (Journal of Medical Laboratory Science/Technology) Vol 4 No 1 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i1.1435

Abstract

Street sweepers, traffic officers, roadside sellers and motorcyclists, including students are subjects who are vulnerable to exposure to vehicle fumes. One of the air pollution generated from motor vehicle fumes is lead (Pb) which is toxic to humans and is accumulative. This study aimed to analyze the relationship between characteristic factors and lead levels in hair using the Atomic Absorption Spectroscopy (AAS) method. Respondents in this study were 32 male university students. The results showed a significance value of 0.274 (p> 0.05) on the parameter of PPE use, 0.049 (p <0.05) on the mileage parameter, and 0.576 (p> 0.05) on the smoking habit parameter. So it can be concluded that there is no effect of the use of PPE and smoking habits on hair lead levels and there is an effect of mileage on hair lead levels in student respondents.
Efektivitas Kunyit (Curcuma longa Linn) terhadap Esherichia coli dan Bacillus subtilis Chylen Setiyo Rini; Jamilatur Rohmah; Leni Yuroh Widyaningrum
Medicra (Journal of Medical Laboratory Science/Technology) Vol 1 No 1 (2018): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i1.1546

Abstract

Herbal plants that serve as a drug, one of which is turmeric that has a role as an antioxidant, antimicrobial, anti-cancer, digestive disorders. Because it contains compounds curcumin and essential oils. The purpose of this research is to know the optimal concentration of dry extract of turmeric rhizome (Curcuma longa L) in inhibiting the growth of E. coli and Bacillus subtilis. This research method using diffusion agar Kirby Bauer. In this study, turmeric extract at 15% concentration had antibacterial efficacy against Bacillus subtilis (0,7 mm) greater than Escherichia coli (0,63 mm).
Hubungan Kadar Cr dalam Air Tambak terhadap Cr Pada Ikan Nila (Oreochromis niloticus), Ikan Bandeng (Chanos chanos) dan Udang Vaname (Litopenaeus vannamei) di Kawasan Jabon Sidoarjo Ary Andini; Siti Dzurrotul Ainiyah
Medicra (Journal of Medical Laboratory Science/Technology) Vol 1 No 1 (2018): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i1.1547

Abstract

Disposal of Lapindo mud into Porong river had caused environmental polluted which might affect water quality and surrounding especially ponds in Jabon Subdistrict, Sidoarjo city. One of hazardous contaminant that harmful to health was chromium (Cr) metal which had carcinogenic effect. The aim of this study was analyzed Cr levels in ponds water, Nile tilapia (Oreochromis niloticus) flesh, Milkfish (Chanos chanos) flesh, Vaname shrimp (Litopenaeus vannamei) flesh, and analyzed the correlation of Cr levels ponds water to Cr levels of Nile tilapia, milkfish, Vaname shrimp. Results of study shown that Cr levels of all ponds water sample were <0,0201mg/L which indicated safe to consumed because they were lower than standard of water Cr levels about <0,05 mg/L. Result of Cr levels in Nile tilapia flesh and Milkfish flesh had same Cr levels about <0,0004 mg/kg which indicated safe to consumed because they were lower than standard of Cr levels fish flesh about <2,5 mg/kg. Shrimp flesh taken from T3 (0,532 mg/kg ) and T5 (0,461 mg/kg) were higher than standard of Cr levels shrimp flesh about 0,4 mg/kg hence need beware to consume. Correlation analyzed of ponds water Cr levels to Nile tilapia flesh, Milkfish flesh, Vaname shrimp flesh Cr levels shown those were not correlation between both of them because P >0,05.
Kontaminasi Escherichia coli pada Makanan Jajanan di Kantin Universitas Muhammadiyah Sidoarjo Jamilatur Rohmah; Chylen Setiyo Rini; Siti Cholifah
Medicra (Journal of Medical Laboratory Science/Technology) Vol 1 No 1 (2018): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i1.1521

Abstract

One of the places to get food is in the cafeteria. Food made in the canteen can be a cause of foodborne desesase and food poisoning if not properly disposed. The objective was to analyse the quality of food microbiology on canteen food in campus 1, 2, and 4 University Muhammadiyah of Sidoarjo. This study applied the descriptive observational which cross sectional design in canteen food stalls and sampling technique which is total sampling. Tests of canteen food samples were performed using Total Plate Count (TPC). A total of 35 samples of canteen foods from the campus canteen. The results show that positive E.coli contamination on canteen food (canteen campus 1, 2, and 4) was 25 (71.43%). There is a significant difference from the burden of microbial samples of canteen food. This research was conducted to find out personal hygiene and environment, and the cleanliness of the campus should be improved and maintained.
Pengaruh Lama Penggunaan Minyak Goreng Kelapa Sawit terhadap Karakterisasi Trigliserida dan Crude Glycerol Intan Febiola; Galuh Ratmana Hanum
Medicra (Journal of Medical Laboratory Science/Technology) Vol 1 No 1 (2018): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i1.1474

Abstract

The price of cooking oil has been increasing every year. Repetaed of using cooking oil will appearance negative impact for healthy and positive impact for biodiesel industry as raw material glycerol. This research aim to knowing the effect of times palm cooking oil for characteristic triglyceride and crude glycerol of pH, flavour, colour, density, and content for daily use. The category of sample is 0 day as control, 4 days as P1, 8 days as P2, 12 days as P3 with experiment laboratory method. The statistic was perfomed by binomial test for flavour, kruskal-wallis test for colour, and one way anova for pH, density, and content. If any treatment effect was continued with post hoc test. The result showed as control; P1; P2; P3 of characteristic triglyceride respectively for pH are 6.67; 6.13; 6.13; 5.88; for flavour is not flavourfull for all type; for colour are yellow; yellow; clear-yellow; brownish-yellow; for density are 0.740; 0.789; 0.747; 0.734; and content are 93.8; 83.9; 80.2; 75.3. whereas characteristic of crude glycerol respectively for pH are 5.92; 6.18; 6.35; 6.28; for flavour is not flavourfull for all type; for colour are yellow; yellow; red-yellow; red; for density are 1.058; 0.961; 1.021; 1.036; and content are 34.4; 46.3; 51.1; 58.4. The result of statistic is times palm cooking oil not influence for pH, but influence significantly for flavour, colour, density, and content.
Uji Aktivitas Antidiabetes Ekstrak Bonggol Nanas (Ananas comusus L.) pada Tikus yang Di induksi Aloksan Ayu Rochmawati; Syahrul Ardiansyah
Medicra (Journal of Medical Laboratory Science/Technology) Vol 1 No 1 (2018): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i1.1473

Abstract

This research aims to knowing about decrease glucose levels in rat induced alloxan with the provision of pineapple stem extract (Ananas comusus L.). Previous studies have shown that bromelin can significantly reduce CD4+ lymphocytes, which are included in inflammatory diseases. One of the inflammatory diseases is diabetes mellitus. Blood glucose levels can be lowered by pineapple extract as it contains bromelin, and bromelin most widely found on the stem. The test animals used white rat strain wistar (Rattus norvegicus L.) with weight is 250-300 gram acclimated for seven days. The research was divided six treatment groups: positive control, negative control, P1 (25% concentration), P2 (50% concentration), P3 (75% concentration), P4 (100% concentration). After treatment for rat in category or sampel was administered pineapple stem extract for 14 days after that experiment status blood glucose level. The results showed that rat had increased (hyperglicemic) after alloxan induced,and also in blood glucose levels in all treatment. the biggest decrease was in P4 is 44 mgdl. The statistic of Kruskal-Wallis test showed that the decrease of rat blood glucose concentration at various concentration of pineapple extract influence signifificantly. According to the result that conclusion is the difference of pineapple extract concentration (25%, 50%, 75%, 100%) influence for decrease blood glucose levels and decreasing blood glucose level after 14 days, but in this time blood glucose not returned as normal conditions.
Efektivitas Anti Bakteri Perasan Bawang Putih (Allium sativum L.) terhadap Pertumbuhan Staphylococcus aureus Siti Mardiyah
Medicra (Journal of Medical Laboratory Science/Technology) Vol 1 No 2 (2018): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i2.1532

Abstract

Garlic has bioactive compounds that are efficacious as antibacterial (bacteriostatic) in some pathogenic bacteria, which one of them is Staphylococcus aureus. Major antimicrobial component is active on garlic especially allicin. The ability of garlic extract as anti-bacterial because of the combination of alisin and scordinin. Alisin serves as a natural antibiotic capable of killing antibiotic-resistant Staphylococcus aureus. This paper aims to study the effect of concentration of garlic juice on the growth of Staphylococcus aureus and determine the minimum concentration to prevent the growth of the bacteria. This type of research is experimental. The population and sample of this study were culture of pure Staphylococcus aureus grown on MSA medium (Manitol Salt Agar). Total sample of research are 28 samples. Staphylococcus aureus growth data were obtained by indirect observation through laboratory tests. Data analysis techniques used Kruskal-Wallis test with 5% error rate (0,05) and Mann Whitney test through SPSS 17,0 program. The result of anti bacterial test of garlic extract (Allium sativum Linn) with Kruskal-Wallis showed that there was influence of extract concentration of garlic on growth of Staphylococcus aureus (p = 0,000). While Mann - Whitney test results obtained that at 25% concentration is the minimum concentration that effectively prevent the growth of Staphylococcus aureus. Therefore it can be concluded that there is influence of extract of garlic juice on growth of Staphylococcus aureus.
Efektivitas Formulasi Ekstrak Daun Sirih Merah (Piper crocatum) Dengan Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius Roxb.) Terhadap Hiperglikemia Serta Histopatologi Pankreas Mencit Hesti Dwi Handini; Jamilatur Rohmah
Medicra (Journal of Medical Laboratory Science/Technology) Vol 1 No 2 (2018): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i2.1477

Abstract

Diabetes mellitus (DM) is metabolism diseases characterized by hyperglycemia and change to the cell histophatology structure of pancreas. Red betel leaves (Piper crocatum) and fragnantpandanleaves (Pandanus amaryllifolius Roxb.) has compound metabolic secondary that efficacious as anantidiabetic. The aims of the study were seek the best extract formulations of red betel leaves (EDSM) and extract of fragnantpandan leaves (EDPW) to lowering blood glucose level, repair the damage of islet langerhans, and comparing the effectiveness with metformin. The research was drafted by post test only control group design using male mencit (Mus musculus) for 31 days. In this research used complete random design with 14 treatment groups, they are normal group, DM group, metfomin group, EDPW 100%group, EDSM 10%+ EDPW 90%group, EDSM 20% + EDPW 80%group, EDSM 30% + EDPW 70% group,EDSM 40% + EDPW 60% group,EDSM 50% + EDPW 50%group,EDSM 60% + EDPW 40% group,EDSM 70% + EDPW 30% group, EDSM 80% + EDPW 20% group, EDSM 90% + EDPW 10% group, and EDSM 100%group. Based on in vivo bioassay showed that metformin have a lot of lowering blood glucose levels (p<0,05) than formulation of EDSM and EDPW but not better in improving islet langerhans tissue damage and treatment DM group with EDSM 50% + EDPW 50% effective lowering blood glucose level comparable with metfomin (p>0,05) and better in improving islet langerhans tissue damage caused by alloxan diabetogenic compound.
Efektivitas Temu Kunci (Boesenbergia rotunda) Terhadap Penurunan Kadar Formalin Pada Ikan Tuna (Thunnus sp.) Zamhariroh Zamhariroh; Galuh Ratmana Hanum
Medicra (Journal of Medical Laboratory Science/Technology) Vol 1 No 2 (2018): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i2.1650

Abstract

Tuna as a marine fish that has an exceptional potential to be the mainstay of Indonesia. The high demand for tuna makes a great opportunity for Indonesia as a producer in the export of these products. Related to this condition, the producers have come up with a variety of ways to keep the fish appear fresh by way of preservation, one of them is by using formalin. Finger root plants have active compounds that can reduce formalin in tuna saponins. This study used tuna fish that had been soaked with a solution containing 10% formalin. The research was conducted at Surabaya City Health Office. Formalin content was measured using the spectrophotometer method. Parameters used were formalin content. The results of this study indicate that the concentration of key solution and storage duration significantly influence at 50% concentration for 5 days is the most effective concentration and duration of immersion with an average yield of 0,04 mg/l.

Page 3 of 9 | Total Record : 89

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