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HAYATI Journal of Biosciences

hayati
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Published by Institut Pertanian Bogor

ISSN : 19783019 EISSN : 20864094 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.

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Articles 8 Documents

Search results for , issue "Vol. 26 No. 4 (2019): October 2019" : 8 Documents clear

Physiological Value of Heart Rate, Respiration and Rectal Temperature of Wild-Captive Dugong (Dugong dugon)–A Case Study in Lingayan Island Aryani Sismin Satyaningtijas; Agik Suprayogi; Ardiansyah Nurdin; Huda S. Darusman
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

This study aims to obtain the physiological value of dugongs that live in natural habitats (in-situ) as protected wildlife, namely on the coast of Lingayan Island, Toli-Toli Regency. Wild dugongs caught on the beach were put into a net cage. After 14 days of living in a beach cage, measurements of heart rate, respiration, and body temperature were measured using non-invasive methods. This study showed that the value of heart rate, respiration, and body temperature were measured (80.00±17.32) beats/minutes, (17.33±6.80) inspiration/minute, and (32.75±0.07)°C. This physiological value is higher when compared to dugongs that live in captivity, this is likely due to the process of homeostasis through physiological adaptation mechanisms. The physiological value of the dugong is very important for the advancement of dugong animal health science and technology. Besides this finding can be a medical reference that is very useful for veterinarians in the diagnostic and therapeutic process.

Antimicrobial Activity of Sonneratia ovata Backer Laili Khumaidah; Adi Setyo Purnomo; Sri Fatmawati
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Sonneratia, a genus of mangroves from family Lythraceae, is reported for number of high biological activity including antimicrobial. Sonneratia ovata Backer is one of the species which traditionally used by Indonesian people for the treatment of some diseases. In this research, the investigation about biological activity of S. ovata Backer as anti-microbial has been performed. The methanol extract of S. ovata Backer is highly potential as antimicrobial against gram positive bacteria, S. aureus, with IC50 value of 7.7 μg/ml which is higher than of Ampicillin as positive control with IC50 value of 37.8 μg/ml. Furthermore, stigmasterol one of the major compound of S. ovata Backer has been isolated from the methanol extract of the stem bark of S. ovata Backer. Therefore, S. ovata Backer is proven to have high activity as antimicrobial, and highly potential to be a new source of antimicrobial agent.

Morpho-Agronomical Diversity of Forest Clove in Moluccas, Indonesia Asri Subkhan Mahulette; Hariyadi Hariyadi; Sudirman Yahya; Ade Wachjar; Ilyas Marzuki
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Forest clove (Zyzygium aromaticum) is a type of wild clove found in Moluccas. The increasing interest of farmers in cultivating this plant leads to complete their information of morpho-agronomical characteristics which could provide diversity reflecting morpho-agronomical their distribution areas. This study aimed to characterize the morpho-agronomical traits of Forest clove plants in Moluccas. By survey, Forest clove more than 15 year old tree totalling of 50 populations were observed their 54 morpho-agronomical characteristics in two areas (Ambon and Seram) from March to June 2018. Two aromatic cloves, namely Tuni and Zanzibar were used for comparative analysis. The results showed that Forest cloves had a similarity of 78% among the population and grouped into 3 groups with a morpho-agronomical variation of 22%. On the contrary, Forest cloves and comparators (Tuni and Zanzibar) had morpho-agronomical differences of 58%. Based on the main component analysis, there were 11 most influential characters of Forest cloves (leaf size index, leaf length, leaf width, leaf area, petiole length, flower length, diameter of flower tube, ripe flower weight, fruit length, fruit width, and fruit weight) which could be descriptors for this plant species.

Protective Effect of Green Algae Ulva reticulata Against Pseudomonas aeruginosa in Drosophila Infection Model Firzan Nainu; Rangga Meidianto Asri; M. Natsir Djide; Muhammad Ahsan; Rudi Arfiansyah; Sartini Sartini; Gemini Alam
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

The emergence of antibiotic-resistant Pseudomonas aeruginosa is one of main health issues in global communities. To overcome such threat, the discovery of novel antibacterial agents is indispensable. This study aimed to evaluate the in vivo antipseudomonal activity of Ulva reticulata extract in Drosophila model of infection. Ethanolic extract of Ulva reticulata was prepared using maceration method and the extract was subsequently assessed for its in vivo antibacterial effect against P. aeruginosa using survival assay, bacterial load enumeration, and gene expression analysis in the wildtype Drosophila. Survival and bacterial load analysis were further performed in a similar fashion on the mutant flies devoid of component responsible in the activation of immune responses against P. aeruginosa. Decline in the survival of infected host accompanied by augmentation of bacterial proliferation was documented in the wildtype Drosophila upon infection with P. aeruginosa. These phenotypic events were further amplified in immune-deficient mutant Drosophila. Nevertheless, improvement of host survivorship and reduction of bacterial burden were demonstrated in both wildtype or immune-deficient mutant flies upon treatment with Ulva reticulata extract after bacterial challenge. Our data demonstrated in vivo antipseudomonal activity of Ulva reticulata extract and thus provide a valuable information about its future potential for health promotion.

Oil Palm Phytochrome-Interacting Factor4 (PIF4) Gene is Conserved and Highly Expressed During Somatic Embryogenesis Mantira Suksirt; Kamolwan Khianchaikhan; Mya Thuzar; Supachai Vuttipongchaikij; Chatchawan Jantasuriyarat
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Oil palm is used in food, fuel and cosmetic industries. Tissue culture is the best way to propagate oil palm; unfortunately the somatic embryogenesis during tissue culture takes long time. The molecular mechanism of somatic embryogenesis in oil palm remains unknown. Recent research reported that auxin plays an important role in early and post-embryogenic plant. PHYTOCHROME-INTERACTING FACTOR4 (PIF4) regulates levels of auxin and the expression of key auxin biosynthesis genes. Our research aims to characterize oil palm PIF4 gene. Thus, we cloned EgPIF4, analyzed the domain using bioinformatic and examined the expression of EgPIF4 during somatic embryogenesis at different tissue including callus and somatic embryo stages; globular, torpedo, cotyledon, and plantlet stage using real-time PCR method. The result showed that EgPIF4 gene comprised 1,737 bp with 9 exons, which encode 578 amino acid residuals. It contains a conserved domain called basic helix-loop-helix domain. EgPIF4 has high level of expression at somatic embryogenetic stage specifically globular and torpedo stage suggested that EgPIF4 plays an important role during somatic embryogenesis. The future characterization of EgPIF4 function in oil palm will help to understand somatic embryogenesis process and facilitate the improvement of the oil palm tissue culture.

Stability and Vulnerability of Bird Flocking Behaviour: A Mathematical Analysis Bayu Erfianto; Intan Muchtadi-Alamsyah
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Given a large number of birds in the flock, we mathematically investigate the mechanism the birds move in a collective behavior. We assume that each bird is able to know its position and velocity of other birds within a radius of communication. Thus, to be able to fly in the flock, a bird has to adjust its position and velocity according to his neighbors. For this purpose, first of all, we analyze how the connectedness of the bird interaction network affects the cohesion of the stable bird flock. We further analyze a condition when the flock is vulnerable, which is mathematically indicated by means of the presence of an articulation point in bird communication network.

3D Landscape Recording and Modeling of Individual Trees Shafarina Wahyu Trisyanti; Deni Suwardhi; Agung Budi Harto
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

The 3D city/landscape model is digital representation of real environment that can be used for planner or landscape architecture in urban development planning. That model’s more focused on building, whereas vegetation model’s also needed for urban planning simulation. This research purposes are to map existing vegetation and to generate individual trees model in some level of details. The research area is campus of ITB Jatinangor and the used data are orthophoto and DSM from UAV-Photogrammetry technology. Manual segmentation, classification, and NDSM generation process can provide tree information (position, crown diameter, species, height)-as 3D vegetation modeling input. It’s also necessary to provide classification, information, detail level, and visualization of vegetation model according to landscape architecture analysis needs. This research results are 3D vegetation models in LoD 1-3 with differents information based on appearance, geometry, semantic, and topology aspects of CityGML. Models then tested qualitatively based on visualization and sun shadow analysis. For visualization, the used data only able to generate LoD 1 and 2 vegetation model and the minimum LoD required for sun shadow analysis is LoD 2. Terrestrial data, which provide the real form and size of each tree part, is needed to generate LoD 3 vegetation model.

Pre-treatment and Suitable Reagent Enabled a Reliable and Consistent for Molecular Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc4) Listya Utami Karmawan; Fenny Martha Dwivany; Rizkita Rachmi Esyanti; I Nyoman Pugeg Aryantha
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Fusarium wilt which is caused by the soil-borne fungus, Fusarium oxysporum f.sp. cubense (Foc), is one of important diseases in banana plant. Foc tropical race 4 (Foc4) is the most pathogenic race which could infect various banana cultivars including Cavendish cultivar which was previously considered as resistant cultivar. Molecular detection of Foc using PCR analysis is indispensable to determine the race of Foc. We demonstrate a faster DNA isolation procedure described in previous method by substituting sodium acetate precipitation with ammonium acetate precipitation without affecting the result. Based on our experience, some fungal genomes were troublesome to be amplified. We suggested pre-treatment step prior to amplification procedure by incubating fungal DNA in 65°C for 10 minutes for any samples of fungal genome, including stubborn samples, before mixing into PCR mix reagent. PCR reagents should be tested for stubborn samples since some of the reagents were unable to amplify the desired DNA fragment. Pre-treatment and the choice of robust PCR reagent should be taken into consideration for a reliable and consistent Foc4 molecular detection result.

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