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Sutiman Bambang Sumitro
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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 15 Documents
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Search results for , issue "Vol 15 No 2 (2010): June 2010" : 15 Documents clear
ISOLASI DAN KARAKTERISASI ENZIM b-1,3-ENDOGLUKANASE DARI TANAMAN KUBIS (Brassica oleracea cv. Capitata L.) Y. Sri Wulan Manuhara
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1627.165 KB) | DOI: 10.23869/261

Abstract

Isolation and characterization of β-1,3-endoglucanase from cabbage (Brassica oleracea var. capitata L.) have been done. It showed 40° C of optimum temperature, and optimum pH is 7. After the purification with hydrophobic interaction chromatography and ion exchange chromatography, it’s activity was increased. Based on SDS-PAGE analysis, β-1,3-endoglucanase have molecular weight around 48 kD. Antifungal activity of β-1,3-endoglukanase show that it has best inhibition zone on Fusarium solanii at extract from ion exchange chromatography.
CHITINOLYTIC BACTERY ACTIVITY ISOLATED FROM THE MUD FIELDS Nuniek Herdyastuti; Sari Edi Cahyaningrum; Tri Joko Raharjo; Mudasir; Sabirin Matsjeh
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/262

Abstract

A chitinolytic microorganism had been isolated from mud fields in Ketintang Surabaya area by using a minimal media containing 0.4% colloidal chitin. Activity assay is based on released N-acetyl-glucosamine which reacted with 3,5 dinitrosalicyclic acid. Mud fields produce 63 bacterial isolates with chitinase activity (TNH1 – TNH63 isolates). The highest activity was shown by TNH11 isolate with specific activity 1.27 U/mg. TNH11 isolates was Gram negative, rod-cocoid cell, has a colony of yellow, round shape, convex elevation, flat margin and the colony diameter 3–5 mm.
Isolasi, Pemurnian dan Karakterisasi Fitase Bacillus subtilis dari Holiwood Gresik Leny Yuanita; Aline Puspita; Suzana Surodjo; Sri Hidayati; Farid Al Amin; Arif Budiman
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/263

Abstract

The aim of the research were isolation, purification and characterization of Bacillus subtilis phytase from Holiwood Gresik. The research was done in two stages; the first include enzyme isolation, precipitation with amonium sulphate, dialysis, gel filtration chromatography, SDS-PAGE analysis, while second determining optimum pH, optimum temperature, the effect of pH and temperature to enzim stability, the values of KM and Vmax Bacillus subtilis phytase from Holiwood Gresik. The first stage research design were One Shot Case Study and Post Test Only Control Group Design, while the second stage were Post Test Only Control Group Design and Factorial Design. The data being analyzed by one-way and two-way Anova. The results of research showed that Bacillus subtilis phytase has the molecular mass of 36.5 kDa, optimum pH at 6.5–7.0, optimum temperature at 41° C and it was found to be stable for 30 minute incubation at pH 7 or 30° C with 2% or 3% lost of its activity respectively. KM value was 0.62 mM and VMax 0.393 mmol/ml/minute.
KOMPOSISI JENIS LARVA SIDAT (Anguilla spp.) YANG BERMIGRASI KE MUARA SUNGAI PROGO, YOGYAKARTA Agung Budiharjo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/264

Abstract

Progo River is one of the rivers on the Java Island, which empties into the Indian Ocean, are became the entrance of the glass eels from the ocean. Glass eels were entering the river consists of several species. The aim of this research was to study of the species composition of glass eels that migrated into the estuary of the Progo River. Glass eels collected in the estuary in the year 2007–2009 at the lunar date 28 on at 20:00 until 04:00. In the period of 3 years, was successfully collected as many as 1.110 glass eels, which consisted of 3 species of Anguilla marmorata (31.17%), Anguilla bicolor bicolor (64,32%), and Anguilla nebulosa nebulosa (4.50%). Glass eels migrated into the estuary of Progo River during September to June. Glass eel of A. marmorata migrated to the estuary during September to June, A. bicolor bicolor migrated during October to June, and A. nebulosa nebulosa migrated to the estuary only during February to June.
EKSPLORASI DAN KOLEKSI JAMUR (MUSROOM) PADA KAWASAN TAMAN NASIONAL BOGANI NANI WARTABONE, SULAWESI UTARA Arwan Sugiharto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/265

Abstract

The exploration have been done to collect and to know variety and existence of edible mushroom in Toraut Village, Boganinani Wartabone, National Park in North of Sulawesi. The result of identified found 12 mushroom at primary and secondary forest ecosystem. There are in genus of Clitocybe, Ganoderma, Fomes, Piptoporus, Daldinia, Heterobasidium, Phellinus, Polyporus, Auricularia, Trametes, Coltricia. Only 2 edible mushroom become one of the food source for local society, Clitocybe odora and Auricularia sp.
THE DIVERSITY OF TERRESTRIAL ARACEAE IN MT. WATUWILA COMPLEX, SOUTH-EAST OF SULAWESI Ina Erlinawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/266

Abstract

The arums comprise the family of Araceae, including the numerous aroids subfamily, monocotyledonous flowering plants, in which flowers are borne on a type of inflorescence called a spadix. The diversity of terrestrial Araceae in Sulawesi is very poorly documented and understood and remains critically threatened. The aims of this study were to understand the diversity of terrestrial Araceae on Mount Watuwila Complex and to add the number specimen collection in Herbarium Bogoriense. The exploration was conducted by used exploring method, including collection, identification and description. The exploration on Mount Watuwila Complex (170–800 m alt.), May 7–29, 2008 was got 22 number collections of Araceae, consist of 12 numbers of terrestrial Araceae, 10 species, those are Aglaonema simplex Bl., Alocasia macrorrhizos (L.) G. Don, Alocasia suhirmaniana Yuzammi & A. Hay, Colocasia esculenta (L.) Schott, Homalomena spp. (3 species), Schismatoglottis calyptrata (Roxb.) Zoll. & Moritzi, S. plurivenia Alderw and Spathiphyllum commutatum Schott. Schismatoglottis calyptrata (Roxb.) Zoll. & Moritzi is dominant species and Alocasia suhirmaniana Yuzammi & A. Hay is endemic species. The descriptions of each species were made.
ESTIMASI POPULASI KETAM KENARI (Birgus latro ) PULAU SIOMPU Jahidin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/267

Abstract

Estimation of the Birgus latro population on the siompu island applied at the supralitora zona, that is the zona of A (north coastal supralitoral), the zona of B (east coastal supralitoral), the zona of C (south coastal supralitoral), and the zona of D (west coastal supralitoral). Objective study was presume of Birgus latro population and sex ratio on the Siompu Island. This research used capture recapture method. Analysis of the population estimation is use anticipation formula by Petersen while female and male ratio use descriptively analysis. Results of research showed that estimate value of population in each of the zona supralitoral area is unegual that is the zona of A is 7,5 or ± 8 individual, the zona of B is 10 individual, the zona of C is 14.4 or ± 14 individual, and the zona of D is 12 individual. Estimate value of the Birgus latro population size on the supralitoral zona of siompu island is 43.9 or ± 44 individual. Male and female ratio in each that; the zona of A is 4:3, the zona of B is 6:2 or 3:1, the zona of C is 9:3 or 3:1, and the zona of D is 8:1. Male and female ratio of Birgus latro at the supralitoral zona of Siompu Island is 27:9 or 3:1.
PENGARUH ESCHERICHIA COLI DAN GRANULOSIT TERHADAP KADAR REACTIVE OXYGEN SPECIES SECARA IN VITRO Sukarjati; Doddy M. Soebady; Aucky Hinting; Sudjarwo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/268

Abstract

In humans, male genital tract infection has been recognized as one of the causes of infertility. Indicators of the occurrence of genital tract infection are the presence of bacteria during semen culture (bacteriospermia) and the finding of leucocyte of more than 1 million/ml semen (leucotytospermia). Escherichia coli (E. coli) is the most common cause of prostatitis and epididymitis. The objective of this study was to determine the effects of E. coli and granulocytes on Reactive Oxygen Species (ROS) level in vitro. This study comprised of two experiments. In the experiment 1, sperm was incubated with E. coli, and the experiment 2, the sperm was incubated with granulocyte. In those experiments, ROS levels were observed. Spermatozoa were obtained from donor with normal spermatozoa according to WHO (1999). Escherichia coli was obtained by culturing the semen of infertile males. Granulocytes were obtained from donors’ blood. Sperm preparation was made by using Percoll gradient column method. Granulocyte isolation used Histopaque 1077 and 1119. ROS level was detected by means of chemiluminescence method with beta counter device. The result of this study showed that in vitro E. coli had the effect on ROS level, both stimulated by peroxidase (p = 0.000) and PMA (p = 0.006). Granulocyte had effect on ROS level. In peroxidase-stimulated ROS level, there was the effect between spermatozoa and granulocyte-incubated sperm (p = 0.000), granulocyte-incubated sperm and granulocyte (p = 0.002), and sperm and granulocyte (p = 0.000). In PMA-stimulated ROS level, there was effect between sperm and granulocyte-incubated sperm (p = 0.000), sperm and granulocyte (p = 0.000), granulocyte-incubated sperm and granulocyte (p = 0.000). In conclusion, under in vitro experiment, sperm incubated with E. coli and sperm incubated with granulocyte had the effect on the level of the ROS.
VAKSIN INAKTIF Klebsiella pneumoniae DENGAN PERLAKUAN SINAR GAMMA DAN PEMANASAN SUHU 65° C I. Djajanegara; I. Sugoro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/269

Abstract

Klebsiella pneumoniae is one of coliform bacteria which cause human dan mammalian diseases. The bacteria dominate in dairy cow milk which has suffered from mastitis and has resistent on antibiotic. Vaccination is one of aims to prevent the diseases. Nuclear technique could be used to have a vaccine candidate. This research was conducted to get the influence of inactivated K. pneumoniae by gamma irradiated and heat inactivated as vaccine candidate on mice. The treatment were positive control (infected by K. pneumoniae), Negative control (injection by physiologies NaCl solution), 800 Gy (infected by K. pneumoniae has inactivated with 800 Gy), 1000 Gy (infected by K. pneumoniae has inactivated with 1000 Gy), 30’ (infected by K. pneumonia has inactivated with heat 65° C for 30’), and 45’ (infected by K. pneumoniae has inactivated with heat 65° C for 30’). The parameters were physical condition, weight, organ, total number of red, white blood cells, and intraperitoneal macrophage. The results showed that irradiated vaccines were better than heat. % mortality of positive control was 100% but the treatments were 0%. The physic condition of mice was normal for irradiated treatment, but not for negative control and heat treatment. The weight gain of mice after vaccination and challenge test were decreased and back normal after 2 days. The organ condition of mice was normal after vaccination and challenge test. The total number of red and white blood cells showed the same pattern for all treatment after vaccination and challenge test, but the total of intraperitoneal macrophage was increased after 4 hours vaccination. Based on the results showed that irradiated vaccine has potential to develop as vaccine candidate.
STUDI KEAMANAN PANGAN PADA TAHU PUTIH YANG BEREDAR DI PASAR SIDOARJO (KAJIAN DARI KANDUNGAN FORMALIN) Restu Tjiptaningdyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/270

Abstract

A lot of substance circulating in the market used preservative as a mean to lengthen a period to keeping. One of them is formalin, its very dangerous poisonous chemicals for health. The aim of this research was to identify the existence of preservative formalin at white tahu in Sidoarjo markets. This was a discriptif research. Sample intake used stratified random sampling method. As basis for stratify is market of place, are traditional and modern market. Identification of formalin content used FMR (formalin mean reagent), with color different as basis criterion. The result showed that 65.90% white tahu circulate in Sidoarjo markets contained the formalin, and 34.10% of white tahu did not contain formalin.

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