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Journal Microbiology Science

microbiologyscience
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Published by Universitas Muslim Indonesia

ISSN : 2808392X EISSN : 28083911 DOI : https://doi.org/10.56711/jms

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Journal Microbiology Sciences (J. Mic. Sciences) receives writing in the form of research results, scientific studies and brief descriptions relating to Microbiology, such as Molecular and Microbial Epidemiology Clinical Microbiology Medical Microbiology Pharmaceutical Biotechnology and others related to Microbiology Sciencee

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 28 Documents

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ETHNOPHARMACY STUDY OF PLANTS WITH MEDICINAL PROPERTIES IN NORTH MALUKU PROVINCE Cici Rahmayanti Adrias; Herwin Herwin; Jamaluddin Jamaluddin; Rachmat Kosman
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

North Maluku Province, especially in Joubela Village, Morotai Island Regency, South Morotai District, is one of the remote areas located in the eastern part of Indonesia. The tribes that still thrive on the island are the Galela and Tobelo, these tribes are some of the largest tribes in North Maluku, recorded to still preserve many plants as traditional medicine. The research aimed to find out the ethnopharmaceutical plants in the village of Joubela. This present study was conducted through direct observation to people who were known to understand the use of traditional medicinal plants such as shamans, community leaders and the public who have used plants for treatment which were selected through purpose sampling method. The results obtained 15 orders, 20 families, 26 genera and 26 species of plants that have empirical potential as medication and the most widely used plant as traditional medicine was garden shrub (Graptophyllum pictum L.), with the efficacy as an ulcer medicine.

ANTIBACTERIAL ACTIVITY TEST OF COMBINATION OF BETEL LEAF EXTRACT (PIPER BETLE) AND KERSEN LEAF EXTRACT (MUNTINGIA CALABURA) AGAINST ESCHERICHIA COLI BACTERIA Ardy Tanfil T; Ema Hermawati; Ahmad Subagiyo
Journal Microbiology Science Vol 2, No 2 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Escherichia coli or commonly referred to as E. coli is a Gram-negative bacteria that can cause various problems in the human body, the mechanism of resistance built by E.Coli to antibiotics is a reason for the need to look for compounds that have antibacterial potential. Betel leaf and cherry leaf are two types of plants that have the potential to have compounds that can be developed as antibacterial, this study tested the two types of plants using the disc diffusion method. The compounds identified in this plant are alkaloids, saponins, and flavonoids. These three compounds are thought to provide strong antibacterial activity in the samples, although further research is still needed to confirm this.

Cover Volume 1 No.1 2021 Journal Microbiology Science
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

IDENTIFICATION OF ACTIVE CHEMICAL COMPONENTS OF ETHANOL EXTRACT OF PAINTED NETTLE LEAVES (COLEUS SCUTELLARIOIDES L. BENTH) AGAINST GASTROINTESTINAL INFECTION BACTERIA USING TLCBIOAUTOGRAPHY AND AGAR DIFFUSION METHOD Fhadillah Junaedi; Rachmat Kosman; Herwin Herwin
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Infectious diseases are one of the public health problems caused by the transmission and multiplication of microorganisms. The research on the antibacterial activity of painted nettle leaves (Coleus scutellarioides L. Benth) extracts was carriedout to examine the activity and chemical components that provide inhibition against gastrointestinal infection bacteria. The extraction of painted nettles leaves performed maceration methd using a solvent of 96%. The extraction results were examined to determine the antibacterial activity at 1% concentration of the test bacteria: Escherichia coli, Salmonella thypi, and Shigella dysenteriae. The results showed that 96% ethanol extract of painted nettle leaves inhibited Escherichia coli, Salmonella thypi, and Shigella dysenteriae. The research conducted using agar diffusion indicated that painted nettleleaf extract showed activity against bacteria and had highly strong potential with diameters of 6-20 mm. The results of Thin Layer Chromatography (TLC) Bioautography test of ethanol extract of painted nettle leaves using chloroform:methanol eluent (3:1) showed inhibition of Escherichia coli, Shigella dysenteriae, and Salmonella thypi at Rf values of 0.90, 0.80, 0.56. Therefore, the identification test confirmed that the chemical componentsproviding antibacterial are from flavonoids, phenols, terpenoids, alkaloids, and steroidsKey words: Identification of chemical components, painted nettle leaves, gastrointestinal bacteria, TLC-Bioautography and Agar diffusion

OPTIMIZATION OF TEMPERATURE CELLULOSE PRODUCING BACTERIAL ISOLATES FROM DRAGON FRUIT (Hylocereus polyrhizus) Ayyub Harly Nurung; Fairuz Dyah Widowati; Herwin Herwin; Fitriana Fitriana
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Isolation of bacterial cellulose from fruits in traditional market of Makassar have been done. This objective of this research was to determine optimum temperature of isolate bacteria that produce cellulose from Dragon fruit (Hylocereus polyrhizus) using dilution agar method. Isolation of bacteria produced cellulose using Hestrin-Schramm agar (HSA) obtain 5 (five) isolate NB02, NB03, NS01 and NS02. The result of screening activity showed that isolate NB04 has a potential as bacteria produce cellulose. The temperature optimize of NB04 has been done at 25oC and 40oC. Based on research results showed that optimum temperature for NB04 was at 25oC and has highest cellulose content at 1,29 g.

ANTIBACTERIAL ACTIVITY OF TEMBELEKAN LEAF (LANTANA CAMARA L.) EXTRACTS AGAINST ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS Fhahri Mubarak; Herlina Rante; Pratami Yona Putri
Journal Microbiology Science Vol 2, No 2 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

(Lantana camara L.) is one of the wild plants that can be used to treat various diseases such as skin diseases and diarrhea. The aim of the study to determine the antibacterial activity of ethanol extract of leaf (Lantana camara L.) can inhibit the growth of bacteria Escherichia coli and Staphylococcus aureus. This research was conducted in May 2019 at the Makassar College of Pharmacy. The phase of this research is weighing extracts, extract concentration variant manufacturing process and testing the antibacterial activity of the ethanol extract of tembelekan leaves. The analytical method used is the agar diffusion method. The results showed that the Tembelekan leaves taken from Tana Toraja Regency had a inhibitory effect on the growth of Escherichia coli and Staphylococcus aureus at concentrations of 5, 10 and 20%. The inhibition zone formed in Escherichia coli bacteria was 8.61, 11.57 and 18.67 mm and in Staphylococcus aureus bacteria that was 9.42, 11.68 and 17.34 mm.

IDENTIFICATION OF ENDOPYTIC FUNGI COMPOUND ON WHITE WEED LEAVES (Ageratum Conyzoides L.) CONTAINING THE POTENTIAL TO PRODUCE ANTIBIOTICS BY TLC-BIOAUTOGRAPHY. Evi Rofika; Fitriana Fitriana; Herwin Herwin
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

White weed (Ageratum conyzoides L.) is known to have antibacterial elements containing chemical compound such as saponin and flavonoids. This study aimed to examine the antibiotic activity of endophytic fungi isolates of white weed leaves by the TLC-Bioautography method. The results of endophytic fungi isolation of white weed leaves obtained 10 isolates consisting of IFDP 1, IFDP 2, IFDP 3, IFDP 4, IFDP 5, IFDP 6, IFDP 7, IFDP 8, IFDP 9, and IFDP 10. The results of the macroscopic examination of the ten isolates of endophytic fungi found different characteristics. The results of the screening using 9 samples obtained isolates that found activity showing high inhibitory power, such as IFDP 1, IFDP 2, and IFDP 4 isolates. Isolates of IFDP 1, IFDP 2 and IFDP 4 were fermented on MYB medium for 14 days. Then filtering and evaporation were carried out to produce an extract. Isolate fermentate extracts were identified using Thin Layer Chromatography with chloroform eluent: methanol (4:2). The antibiotic activity test was done using TLC-Bioautography method, and obtained an Rf value in isolate 1, at Rf 0.74 on Vibrio cholerae. Isolate 2 resulted rf value of 0.67 on Salmonella thypi, Vibrio cholerae, Eschericia coli, disentriae, Staphylococcus epidermis. Isolate 4 generated an Rf value of 0.74 on Pseudomonas aeruginosa and Vibrio cholerae. While the group of active chemical components contained in the endophytic fungi isolates of white weed leaves was lavonoids by using AlCl3 reagents, AICI3 reagent alkaloids, and Sulfuric acid for saponin identification

ANTIBACTERIAL ACTIVITY OF PATCHOULI OIL REFINERY WASTE AGAINTS STAPHYLOCOCCUS AUREUS BACTERIA Sarmila Sarmila; Abd. Rahman Razak; Jamaluddin Jamaluddin
Journal Microbiology Science Vol 2, No 1 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Patchouli oil refining waste is biomass or organic waste which has chemical contect so that it has antibacterial potential. This study aims to determine the antibacterial activity of patchouli oil refining waste against Staphylococcus aureus and knowing the groups of compounds identified as having antibacterial activity after the TLC Bioautography test was carried out. The sample was extracted using the maceration method with 96% ethanol solvent. Antibacterial activity testing was carried out with a concentration variant of 50%, 25% and 10% with the well method. Thin layer Chromatography (TLC) using n-hexane : ethyl acetate (3:1) elucants. TLC Bioautography using contact bioautography method. From the research that has been done, it is found that the ethanol extract of patchouli oil refining can inhibit Staphylococcus aureus which has the largest diameter of resistance with a concentration of 50% which is categorized as very strong. The results of the TLC Bioautography study showed the saponin compound class after being sprayed with H2SO4 10% reagent, it was found that the ethanol extract of patchouli oil refined pulp has antibacterial activity against Staphylococcus aureus and compounds to play a role in antibacterial activity are saponins.

ANTIBACERIAL ACTIVITY OF ENDOPHYTIC FUNGI ISOLATE OF PAINTED NETTLE (Coleus scutellarioides L. Benth.) AGAINST SKIN INFECTION BACTERIA USING TLC-BIOAUTOGRAPH AND AGAR DIFFUSION METHODS Elivilia Oktaviana Bonita S
Journal Microbiology Science Vol 2, No 2 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Painted Nettle (Coleus scutellarioides L. Benth.) has properties as anti-inflammatory, antioxidant, and antibacterial. It accelerates wound healing as well. Painted nettle contains essential oils, phenols, tannins, phytosterols, alkaloids, and flavonoids. This study aimed to obtain endophytic fungi isolates that have the potential as antibacterial by TLC-Bioautography and agar diffusion methods. This research was conducted by isolating endophytic fungi of painted nettle, advanced to purification and macroscopic tests and nine pure isolates were obtained. The results of the isolate screening with the IFDI code 6 showed the highest inhibition. Furthermore, the antibacterial activity was assayed by TLC-Bioautography. The results obtained two active spots (Rf1 = 0.96; Rf2 = 0.25) against Staphylococcus aureus and Staphylococcus epidemics, three active spots (Rf1 = 0.96; Rf2 = 0.61; Rf3 = 0.25) against Psedudomonas aeruginosa. The results of the antibacterial activity assay by diffusion method obtained inhibitory zone for each bacterium, namely Staphylococcus aureus at 8.95 mm, Staphylococcus epidermidis at 8.24 mm, and Pseudomonas aeruginosa at 7.46 mm.Key words: Antibacterial, agar diffusion, endophytic fungi, skin infections, TLC Bioautography

ISOLATION AND ACTIVITY ANTIBACTERIAL OF ISOLATES ENDOPHYTE FUNGI OF JATROPH MULTIFIDA L. STEM Herwin Herwin; Rachmat Kosman; Sri Wahyuni
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Endophytic fungi that live on plant tissue has the potential to produce secondary metabolites, same as its host. This study aims to isolation endophyte fungi and antibacterial activity of isolate endophyte fungi of Jatropha multifida L. stem to Escheriacia coli and Staphylococcus aureus bacteria. The preliminary research was performed by isolation endophyte fungi of Jatropha multifida L. stem use PDAC medium and the result of isolation endophyte fungi Jatropha multifida L. stem obtained 6 isolates. Isolates endophyte fungi done examination morphology by microskopic method obtained that islolates have different carasteristic. Based on examination of antibacterial activity by agar diffusion method showed that isolates endophyte fungi that potential as antibacterial is isolate IFJT1 code where isolate IFJT1 code active to Escheriacia coli bacteria obtained inhibitory zone diameter of 17,7 mm and to Staphylococcus aureus bacteria.obtained inhibitory zone diameter of 16,7 mm

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Home Page

OAI Link

Editorial Team

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Reviewer

Google Scholar

Contact Name Ayyub Harly Nurung

Contact Email ayyub.harlynurung@umi.ac.id

Phone +6282328980617

Journal Mail Official jmic.sci@umi.ac.id

Editorial Address Editor's Address: 3rd Floor Laboratory Faculty of Pharmacy, 2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia

Location Kota makassar, Sulawesi selatan INDONESIA

Abstract

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Abstract

Contact Name
Ayyub Harly Nurung

Contact Email
ayyub.harlynurung@umi.ac.id

Phone
+6282328980617

Journal Mail Official
jmic.sci@umi.ac.id

Editorial Address
Editor's Address: 3rd Floor Laboratory Faculty of Pharmacy, 2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia

Location
Kota makassar,

Sulawesi selatan

INDONESIA