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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol. 3 No. 4 Oktober 2014" : 8 Documents clear
EKSTRAKSI DAN UJI STABILITAS ZAT WARNA DAUN JAMBU BIJI (Psidium guajava L.) Lasria Pardede; Endang Kusdiyantini; Anto Budiharjo
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Guava leaves is known as a traditional medicine to treat various diseases, such as diarrhea, dengue fever, etc. Along with the development of technology, guava leafs is now used as a color contributor on  textiles. This study aims to extract on the solvent distilled water and ethanol and to test the stability of guava leaf color pigment against the influence of storage temperature, time span of the addition of an oxidant and  pH. Leaves extracted by soaked for 24 hours in distilled water solvent that has been preheated to 30°C, 50ºC, 70º C and 90ºC and ethanol with a concentration of 20%, 40%, 60%, 80% and 96%. Absorbance measurements of guava leaf extract is using spectrophotometer at a wavelength of 525 nm. The results  showed that guava leaf extract has the optimum absorbance value on distilled water solvent at 90ºC and 20%ethanol. Stability test is done by storage temperature effect, oxidizing agents adding, and pH treatment. Stability test of guava leaf extract showed that extracted guava leaf color pigment is stable on 9°C storage temperature, short time oxidant adding (3 hours), and resistant to alkaline pH conditions (pH 9).Keywords: Psidium guajava L., ethanol, distilled water, spectrophotometry
Produksi dan Kestabilan Pigmen Merah Kapang Monascus sp. Menggunakan Media Tepung Kulit Singkong dengan Penambahan Bekatul pada Konsentrasi yang Berbeda Sona Fatimah; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The used of synthetic dyes have a various of negative effects on human health. Pigment produced by Monascus sp. can be used as an alternative natural coloring food. The purpose of this research is to produce pigment Monascus sp. using the substrate cassava peels flour with the addition of rice bran at various concentrations. The study was conducted by growing Monascus sp. on medium cassava peels flour with the addition of rice  bran in various concentrations (0%, 2.5%, 5% and 10%). Measured parameter is the production of red pigment and pigment stability against temperature, water content, solubility and pH. Red pigment production was measured using a spectrophotometer with a wavelength of 500 nm. Analysis of the data using One Way ANOVA and Duncan test. The results showed that the highest production of red pigment (5.6 CVU / gds) and water content of 47% was obtained on medium cassava peels flour with rice bran addition of 10%. Pigment stability towards heat are 0,39 at 300C, 0,35 at 600C, 0,27 at 1210C, and 0,22 at 1500C , stability pH 3 are 0,37 (0 hours incubation) dan 0,26 (12 hours incubation) whereas pH 7 are 0,38 (0 hours incubation) dan 0,36 (12 hours incubation).Keywords: Fermentation,Cassava peels flour, Rice bran, Monascus, Pigment intensity, stability
Produksi Pigmen Kapang Monascus sp. dari Angkak pada Substrat Tongkol Jagung (Zea mays) dengan Variasi Ukuran Substrat dan Kadar Air Sesaria Esa Sekar Ardini; Agung Suprihadi; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Natural dyes in food industry is more demand so its pushes eforts for developing the production. Monascus is one of fungi that is potentially used for anka production that used in industrial products such as meat, fish, soy sauce, and liquor. The aim of this study was to produce pigment from Monascus using corncob with 35 mesh (<0,5 mm), 18 mesh (0,6-1 mm), 10 mesh (1,1-2 mm) particle size substrate and 20%, 40%, 60% moisture content. The production of Monascus sp. pigments made of solid-state fermentation and  incubated for  14 days. The parameters observed were pigment production and biomass. Analysis of pigment using spektrophotometer with wavelength (λ)  of 500 nm as well as biomass analysis by measuring N-acetyl glucosamin. The results of analysis showed that particle with size 0,6-1 mm from the substrates and with 20% and 40% moisture content was the best condition in process of pigment production using corncob substrate. The production of pigment from fungi with substrate with size 18 mesh ((0,6-1 mm) in 20% moisture content reached 1,1 CVU (Color Value Unit)/ml, and  in 40% moisture content reached 1,07 CVU/ml. The highest biomass was reached on 10 mesh (1,1-2 mm)  particle size at 20% moisture content reached 0,83 A (absorbance)/gds (gram dry substrates), 40%  reached 0,72 A/gds, and 60% reached 0,81 A/gds.Keywords: Solid-state fermentation, corncob, Monascus sp., particle size, moisture content
Perkecambahan Biji Dan Pertumbuhan Bibit Batang Bawah Karet (Havea brasiliensis Muell Arg.) Dari Klon Dan Media Yang Berbeda Devi Sara; Munifatul Izzati; Erma Prihastanti
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rubber tree is an economical plant because of its latex production which is used as raw materials in rubber industries. The most important steps in rubber tree cultivation are germination and nurseries of stock for the grafting. The aims of the study to investigate seed germination from  3 types of clone and stock growth from different clone and plant media. The research consists of two steps: 1. to investigate seed germination from 3 types of clone, 2. to investigate growth of stock rubber on different clone and plant media for 30 days. The study design used completely randomized design (CRD) with 4 repetition. The first factor was plant media (soil, soil + manure, soil + liquid fertilizer, soil + ash). Second factor was seed clone (PB 260, GT 1, BPM 24). The pameter measured were germination percentage, stem height, root length, leaf number, leaf area, dry and fresh weight. Collected data were analyzed by Analysis of Variance (ANOVA) continued by Duncan Multiple Range Test (DMRT) in 95% significance difference. The study resulted that BPM 24 clone had the highest germination percentage on 74,9%, while PB 260 is 71% and GT 1 is 63,6%. The types of clone affected on growth of stock rubber tree. The PB 260 clone had better stem height, root length, leaf number, leaf area, dry and fresh weight parameter than the other clone. Plant media had no effect on all parameters. There is no interaction between plant media clone in affecting to growth of stock rubber.Key Words: Clone, PB 260, GT 1, BPM 24, manure, liquid fertilizer
Produksi Pigmen Merah Monascus sp. pada Substrat Tepung Biji Durian (Durio zibethinus Murr.) dan Biji Nangka (Artocarpus heterophyllus Lam.) dengan Variasi Penambahan Sumber Nitrogen Tsania Dyna Falasifa; Agung Suprihadi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Public awareness about the danger of using synthetic dyes impact on health for a long term, pushes the demand of natural dye that is healthier. One of the natural dye is Monascus sp. red pigment which traditionally uses the rice substrate. Durian seed and jackfruit seed are residue from agroindustry which still have high nutritional value thus potentially become the alternative substrate. The aim of this study was to measured Monascus sp. red pigment production in the flour substrate of durian seed and jackfruit seed with variant nitrogen source added. This research used completely randomized design (CRD) factorial with 2 factors. The first factor was a kind of substrate fermentation (N): jackfruit seed flour and (D): durian seed flour. The second factor was concentration peptone added (P0): 0%, (P1): 1%, (P2): 2%, and (P3): 3%. Research consist of 8 treatments with 3 replications. Research parameters were red pigment concentration and biomass estimation. Analysis of the data used Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT) at the 95% significance level and Pearson correlation test. The result showed that Monascus sp. can  grew and producted red pigment on both of substrate with the addition of a nitrogen source variation. Fermentation on the jackfruit seed flour substrate with 2% peptone added was the most effective in red pigment production  with 5,55 CVU/gds. The addition of 2% peptone on both types of substrate yielded highest biomass with 1.53 mg/gds, and there was a positive correlation between the biomass and the red pigment production. Keywords: Pigment, Monascus sp., Durio zibethinus, Artocarpus heterophyllus, nitrogen
ISOLASI DAN KARAKTERISASI ISOLAT KONSORSIUM BAKTERI LAHAN PERTANIAN SEBAGAI POTENSI DEGRADASI PESTISIDA PROPOXUR Ahmad Qi Sahlan; Endang Kusdiyantini; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Propoxur pesticide is a pesticide used widely enough by the Indonesian agricultural community. This pesticide is used as a systemic pesticide in agricultural areas to deal with pests as well as insecticides, herbicides, and bactericide. Study of the degradation of propoxur has long performed using the bacterial bioremediation agent. Bacterial consortium is a combination of several bacteria in a medium which that has a complementary function in the environment. The purpose of this study was the isolation and characterization of a consortium of farms that use propoxur pesticide. Isolation was done by spread plate and was observed characteristics of colony morphology. The obtained isolates were tested against propoxur and its potential role in the consortium. Degradation of propoxur observations using High Performance Liquid Cromatography (HPLC). Isolation results obtained 8 (eight) bacterial isolates and one that have an important role in the consortium is K1 isolate. K1 isolate were able to work up a consortium to degrade Propoxur in 2 isolates with K2 isolate. The interaction of these bacteria are natural and have a tendency to vary in nature after a process of isolation. K1 isolate are not able to work alone in a mineral medium, and worked with synergistic and complementary that in the bacterial consortium. KSR consortium optimum growth occurs in an environment with temperature 35 °C and pH 6.Keywords: Isolation, Propoxur, Bioremediation, Bacterial Consortium, HPLC
KEANEKARAGAMAN DAN KEMELIMPAHAN LARVA INSEKTA AKUATIK SEBAGAI BIOINDIKATOR KUALITAS AIR DI SUNGAI GARANG, SEMARANG Sitta Maulina Marpaung; Fuad Muhammad; Jafron Wasiq Hidayat
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

River is a natural habitat of macrobentik organism which is include the larvae of aquatic insects. The larvae of aquatic insects can be used as bioindicators of water pollution because they are can accomodate the change of the environmental. The purpose of this research is determine the community structure of aquatic insect larvae as well as its aspect bioindicators. This research used survey method and sampling techniques by purposive sampling. Location of the research consisted of four stations determined based on area’s function along the Garang River. At each station represent by three samples taken with surber mesh size 25 x 40 cm. The result this research showed that the larvae of insects were found in every observation station which is consists 15 genera, i.e namely Heptagenia, Stenacron, Baetis, Cloeon, Procleon, Caenis, Glossosoma, Hydropsyche, Cheumatopsyche, Chimarra, Tinodes, Chironomus, Chrysop, Isoperla and one of the family Culicidae was unidentified. The highest diversity found at Gebugan village ) in the rice fields (the diversity value of 2.17. The lower diversity at Tinjomoyo street with only reached diversity make 1,45. The conclusions of this study grouped Garang River that be into two categories, they are moderately polluted and contaminated. Heptagenia and Baetis categorized as positive bioindicator forests, fields and settlements, where as negative bioindicator industrial area are Procleon and Glossosoma. Keywords : Aquatic insect larvae , Garang River , Bioindicator
EKSPLORASI DAN KARAKTERISASI BAKTERI POTENSIAL PENGHASIL SENYAWA ANTIFOULING YANG BERASOSIASI DENGAN ALGA COKLAT (PHAEOPHYTA) DI PERAIRAN KEPULAUAN KARIMUNJAWA JEPARA Barri Pratama; Endang Kusdiyantini; Agung Suprihadi; Anto Budiharjo; AB Susanto
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Biofouling is a settlement process from marine organisms initiated by biofilm (microbial layers). Corrosion on the vessels, pipelines under the sea, oil plants, etc are big problems in the industrial today cause adversely affects. Antifouling paints have been developed to prevent settlement of organisms, however antifouling paints contain heavy metals and biocides which have toxic effects on marine ecosystems. Antifouling compound which environmental friendly is a solution for one of the paint component. The aim of this research was to isolate bacteria associated with brown algae which had potential to produce antifouling compound from Karimunjawa islands, Jepara.Isolate KS1-1 showed antifouling activity against Pseudomonas aeruginosa as biofilm-forming bacteria.This isolate had been characterized in morphology, moleculer and biochemichal identification.Moleculer analyses of 16S rRNA sequence, KS1-1 showed similarity 94% from 287 bp with Vibrio sp. W-137-16S ribosomal RNA gene, partial sequence. Keywords : Antifouling, Phaeophyta,Biofouling, 16S rRNA, Vibrio

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