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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
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Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 20 Documents

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Search results for , issue "Vol 12, No 1 (2007): February 2007" : 20 Documents clear

Sistematik Numerik Strain-Strain Anggota Genus Pseudomonas Pendegradasi Alkilbenzen Sulfonat Liniar Berdasarkan Sifat Fenotip dan Protein Fingerprinting Suharjono, Suharjono; Sembiring, Langkah; Subagja, Jusup; Ardyati, Tri; Lisdiana, Lisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Bacteria strains consisting of Pseudomonas sp. strain J and R isolated from river ecosystem polluted and Pseudomonas sp. strain A and B isolated from river ecosystem unpolluted by detergent were capable to degrade of LAS. The objective of this research was to determine similarity value by numerically of LAS-degrading Pseudomonas strains based on phenotype character and protein fingerprinting using three reference strains consist of Pseudomonas putida FNCC071, P. fluorescens FNCC070, and P. aeruginosa FNCC063. Phenotype characteristics examined are cellular and colony morphology, biochemical nature, capability to degrade polysaccharide, tolerance to various environmental factors and antibiotics, and ability to ferment sugar. Cellular protein fingerprinting was analyzed using SDSâ€“PAGE discontinuous. Strains classification was determined based on Simple Matching Method similarity index by UPGMA (Unweight Pair Group Method with Average) algorithm. Based on phenotype nature, all strains have similarity value 0.61; however, based on cellular protein fingerprinting, those strains have similarity value 0.52. All strains of LAS-degraded were including in the genus of Pseudomonas.

Kebutuhan Protein/Asam Amino dan Karbohidrat Bagi Udang-Udang Penaeid: Suatu Studi Literatur Rosyida, Eka
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Suatu studi literatur dilakukan untuk menelaah kebutuhan protein/asam amino dan karbohidrat bagi udang-udang penaeid, suatu jenis udang yang bernilai ekonomis penting di Indonesia maupun di pasaran dunia. Salah satu hal penting dalam membudidayakan udang tersebut adalah berkaitan dengan kebutuhan nutrisi pada fase larva. Pemberian nutrisi yang buruk dianggap secara langsung maupun tidak langsung dapat menyebabkan timbulnya beberapa penyakit, kematian massal dan penurunan produksi. Kebutuhan protein udang penaeid mempunyai korelasi dengan aktivitas enzim proteolitik dan dipengaruhi oleh umur, ukuran dan tahapan/fase dari siklus hidup udang tersebut. Faktor kualitas dan tingkat energi dari sumber protein yang diberikan secara signifikan berpengaruh terhadap pertumbuhan udang, sedangkan disakarida dan polisakarida dianggap lebih efisien sebagai sumber karbohidrat untuk udang penaeid dibanding monosakarida.

Pengaruh Jarak Penyekat dalam Fotobioreaktor Tubular terhadap Pertumbuhan Kultur Mikroalga Ankistrodesmus convulutus Chrismadha, Tjandra; Rustiani, Tenni; Rosidah, Rosidah; Mardiati, Yayah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

A series of partition was constructed inside a vertical tubular photobioreactor to control the culture distribution in obtaining intermittent exposure to light area in the surface of the tubular column. A microalga, Ankistrodesmus convulutus, was grown in a batch mode in the reactor with PHM medium, light sources of fluorescent lamp 3 x 40 watt (I = 5.500 lux on the surface of the tubular) and room temperature of 27 â€“ 310C. The experiment was carried out to study the influence of partition density which was constructed at varied distance, which were 1, 2 and 3 inch on the growth and biochemical composition of the alga. A vertical tubular photobioreactor without partition was applied as the control. The partition density did not significantly affect the optical density achievement of the culture, but it significantly affected the biomass and chlorophyll concentration, as well as the protein/carbohydrate ratio of the culture. It is believed that the intermittent exposure of algal cell to the light area leads to sincronization of the light and dark reaction of the photosynthesis, as well as to vapor the catalitic proccesses to develop the functional cell structure.

Pseudo-Nitzschia pseudodelicatissima Isolated From Hurun Bay: Salinity Tolerance and Domoic Acid Content Panggabean, Lily
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Pseudo-nitzschia pseudodelicatissima dari sampel perairan Teluk Hurun, Lampung berhasil diisolasi dan dikultur dalam medium F/2 dengan penyinaran 2500 luks 12 jam per hari pada suhu 24-250C. Perlakuan salinitas menunjukkan bahwa P. pseudodelicatissima tidak toleran terhadap salinitas kurang dari 15 PSU dan sangat toleran terhadap salinitas yang tinggi hingga mencapai 45 PSU (Pressure Salinity Unit). Salinitas optimum untuk pertumbuhan P.pseudodelicatissima yaitu pada 30 dan 35 PSU. Analisis ekstrak P. pseudodelicatissima, 9 hari sesudah inokulasi, menggunakan HPLC-UV tidak menunjukkan adanya kandungan asam domoat.

Pengaruh Variasi Fotoperiodisitas terhadap Pertumbuhan Chlorella dalam Medium Basal Bold Prihantini, Nining; Rachmayanti, Winny; Wardhana, Wisnu
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

The research of photoperiodicity effect on the cell densities of genus Chlorella grown in Boldâ€™s Basal Medium (BBM) had been done. Observations were done for 14 days. Research was experimental study with full random design to 8 varieties of photoperiodicity i.e. 6 h light/18 h dark (L/D) cycles, 8 h light/16 h dark (L/D) cycles, 10 h light/14 h dark (L/D) cycles, 12 h light/12 h dark (L/D) cycles, 14 h light/10 h dark (L/D) cycles, 16 h light/8 h dark (L/D) cycles, 18 h light/6 h dark (L/D) cycles, and 24 h light/0 h dark (L/D) cycles. On peak culture, 24 h light/0 h dark (L/D) cycles produced the highest cell numbers (204.680.000 cell/ml) and the lowest cell numbers were achieved by culture with 6 h light/18 h dark (L/D) cycles. Kruskall-Wallis test showed that there were some effects of photoperiodicity variations on cell numbers of Chlorella (cell/ml) in culture (p>0.05). Multiple comparison tests showed that mean of cell numbers of Chlorella (cell/ml) differ (p>0.01) on every photoperiodicity. Relationship between photoperiodicity and cell numbers of Chlorella was determined by regression equation Å¶ = 24634821,214 + 21977643,869 X.

Eksplorasi dan Bioasai Berbagai Isolat Bacillus thuringiensis Lokal terhadap Larva Beberapa Jenis Serangga Suryanto, Dwi; Chairani, Chairani; Rusika, Deddy; Lubis, Nita A.; Yurnaliza, Yurnaliza
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Nine isolates of Bacillus thuringiensis of North Sumatra have been isolated from soil and dead larvae of Plutella xylostella. Bioassay of these isolates to larva of Heliothis armigera, Plutella xylostella, Aedes aegypti, and Culex sp. showed that the isolates have different spectrum and ability in controlling insect larvae. Interestingly, TU1 has similar ability to that of Dipel (B. thuringiensis var kurstaki strain HD-7) in controlling Dipteran (A. aegypti and Culex sp.) and Lepidopteran (H. armigera and P. xylostella). The others were not effective to Dipteran, but have various abilities to kill Lepidopteran. This result indicated that local isolates of B. thuringiensis of North Sumatra were varied in ability to kill different larvae.

Perilaku Makan Badak Sumatera di Suaka Rhino Sumatera, Taman Nasional Way Kambas, Lampung Suharto, Ignatius; Rustiati, Elly Lestari; Adi, Marcelus
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Research on the feeding behaviour, feeding plants and the way to access the plants has been conducted on Sumateran rhinos at and in collaboraton with Sumatran Rhino Sanctuary, Way Kambas National Park, Lampung, by scanning and rapid assessment methods. Feeding activity is done during day time, especially in the morning (05.00-10.00) and in the afternoon (14.00-18.00). Plants consumed include saplings (42%), followed by trees (30%), seedlings (20%) and liana (8%). Cutting (86%) is the most common way to access the food sources, followed by pulling (9%), pushing by its head and holding in between front legs (4%) and twisting by its horn (1%).

Seleksi Dan Identifikasi Lactobacillus Kandidat Probiotik Penurun Kolesterol Berdasarkan Analisis Sekuen 16s Rna Triana, Evi; Nurhidayat, Novik
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

High fat and low fiber dietary pattern results in raising of blood cholesterol level over the normal level, namely hypercholesterolemia. Hypercholesterolemia might cause coronary disease and stroke. Blood cholesterol is able to be decreased by probiotic supplement. Lactobacillus is one of the probiotics that were well known and taken advantages. However its role as cholesterol lowering agent was less known. Therefore, screening and identification of Lactobacillus isolates which were candidates of probiotic have been carried out. Isolates Mar 8, Lac 3 and 7 p have been selected as Lactobacillus candidates for cholesterol lowering probiotic. Those isolates met criteria for cholesterol lowering probiotics. Furthermore, they have been conducted to confirm their identity as Lactobacillus. 16S RNA sequences analysis by BLAST analysis against reference strains within DNA Data Bank of Japan (DDBJ) have been carried on. Results showed that sequences of Lactobacillus Mar 8 was 100% homology with Lactobacillus plantarum, Lac 3 was 100% homology with Lactobacillus paracasei and 7 p was 99% homology with Lactobacillus plantarum. It was concluded that the three isolates were selected as candidates for cholesterol lowering probiotics. Both of them, Mar 8 and 7 p, are Lactobacillus plantarum. Another one, Lac 3 is Lactobacillus paracasei.

Uji Aktivitas Antibakteri dan Sitotoksik Ekstrak Metanol Aglaia silvestris (M.Roemer) Merr. Praptiwi, Praptiwi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

The antibacterial and sitotoxic activities of ganggo (Aglaia silvestris (M.Roemer) Merr. methanol extract were determined in this study. Antibacterial in-vitro test of ganggo methanol extract was exposed to Gram positive (Staphylococcus aureus ATCC 25923, Streptococcus agalactiae ATCC 8190, and Streptococcus sp.) and Gram negative (Salmonella typhii NCTC 786 E, Eschericia coli ATCC 25922, and Pseudomonas pseudomallei ATCC 15682) bacteria. The extract concentrations were 50, 25 and 12.5%, and done in triplicate. The growth inhibition area of extract was compared to those of standard antibiotic (10 unit ampicilin). Cytotoxic test of ganggo extract was done utilizing Brine Shrimp Lethality Test (BST) with Artemia salina.The result showed that growth inhibition area of 12.5% ganggo methanol extract to P. pseudomallei (19 mm) was wider than that of 10 unit ampicilin (0 mm). It showed that P. pseudomallei was sensitive to ganggo methanol extract. The result of BST showed that LC50 of ganggo extract was 345.44 ppm. It was concluded that ganggo methanol extract had antibacterial effects on some bacteria isolates and had cytotoxic effects with LC50 345.44 ppm.

Aktivitas Trypsin Inhibitor Berbagai Varietas Biji Kedelai (Glycine max L.) dan Perubahannya Selama Perkecambahan Biji dari Varietas Terbaik Kanetro, Bayu; Noor, Zuheid; Indrati, Retno
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i1.2529

This study investigated the activity of trypsin inhibitor (TI) of some soybean (Glycine max L.) varieties and the change in TI activity during seed germination of the best variety. The research was aimed to determine the best variety of soybean and germination time based on the highest TI activity. There were 5 varieties of soybeans, Paderman, Argomulyo, Kaba, Sinabung, and Ijen. The best variety of soybean was Sinabung as shown by the highest TI activity. The soybean of Sinabung variety was germinated for 6 various germination times at 12, 24, 36, 48, 60 and 72 hr. The result showed that the variation of germination time changed TI activity. TI activity decreased significantly after 36 hr of germination of soybeans. The best time of germination was 36 hr.

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Contact Name
Brigitta Laksmi Paramita

Contact Email
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+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA