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All Journal JURNAL TEKNOLOGI LINGKUNGAN Media Penelitian dan Pengembangan Kesehatan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Buletin Kebun Raya BERITA BIOLOGI Jurnal Penelitian Pembelajaran Fisika MAGNA MEDICA: Berkala Ilmiah Kedokteran dan Kesehatan Teknologi Indonesia
Praptiwi Praptiwi
Puslit Biologi LIPI, Jl. Raya Jakarta-Bogor Km. 46 Cibinong 16911 Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Physics Public Health

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3-ACETYL -2,5,7-TRIHYDROXY-1,4-NAPHTALENEDIONE, AN ANTIMICROBIAL METABOLITE FROM THE CULTURE OF ENDOPHYTIC FUNGUS COELOMYCETES TCBP4 FROM Tinospora crispa Praptiwi, Praptiwi; Jamal, Yuliasri; Fathoni, Ahmad; Nurkanto, Arif; Agusta, Andria
Media Penelitian dan Pengembangan Kesehatan Vol 23, No 3 Sep (2013)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

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Abstract

Abstract Isolation, identification and testing of antimicrobial activity of secondary metabolites of endophytic fungal culture TCBP4 isolated from bratawali (Tinospora crispa) has been performed. The fungus TCBP4 was cultivated in Potato Dextrose Broth (PDB) for 1 month, media and fungi extracted with ethyl acetate. The extract was separated and purified by several chromatographic techniques, from which 9 fractions were obtained. Fraction 3e was purified again and was obtained 6 fractions (3e1-3e6). Fractions 3e3-3e6 were tested against bacteria isolates Staphylococcus aureus Bacillus subtilis,Eschericia coli and yeast isolate Candida albicans by microdillution method. Antimicrobial activity test result showed that fractions 3e3-3e5 had better antibacterial activity compared to chloramphenicol as commercial antibiotic. It was indicated by MIC value of the fractions was lower (8 ug/ml) compared with the antibiotic chloramphenicol (16 ug/ml). Fraction 3e3 had better antifungal activity compared to commercial antifungal nystatin and cabisidin against C. albicans. GC-MS analysis showed that the chemical constituent of 3e5 fraction was identified as 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione . Key words : Tinospora crispa, endophytic fungi, isolation, identification, antimicrobial Abstrak Isolasi, identifikasi dan uji aktivitas antimikrobia terhadap metabolit sekunder dari kultur jamur endofit TCBP4 yang diisolasi dari tumbuhan bratawali (Tinospora crispa). Jamur endofit TCBP4 dikultivasi pada media Potato Dextrose Broth (PDB) selama 1 bulan, selanjutnya media dan jamur diekstrak dengan pelarut etil asetat. Ekstrak dipisahkan dan dimurnikan dengan beberapa teknik kromatografi sehingga diperoleh 9 fraksi. Fraksi 3e dimurnikan kembali dan diperoleh 6 fraksi (3e1-3e6). Fraksi 3e3-3e6 diuji aktivitas antibakterinya terhadap beberapa isolate bakteri Staphylo-coccus aureus Bacillus subtilis, Eschericia coli dan isolate khamir Candida albicans dengan metode mikrodilusi. Hasil uji antimikrobia menunjukkan bahwa fraksi 3e3-3e5 mempunyai aktivitas antibakteri lebih baik bila dibandingkan dengan chloramfenikol, dimana nilai MIC dari fraksi (8 ug/ml) lebih rendah dari antibiotika chloramfenikol (16 ug/ml). Fraksi 3e3 mempunyai aktivitas antijamur lebih baik bila dibandingkan dengan antijamur komersial nistatin dan kabisidin terhadap  C. albicans. Analisis GC-MS menunjukkan bahwa komponen kimia dari fraksi 3e5 diidentifikasi sebagai 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione . Kata kunci : Tinospora crispa, jamur endofit, isolasi, identifikasi, antimikrobia
UJI ANTIBAKTERI Lasianthus (RUBIACEAE) SEBAGAI TUMBUHAN BERKHASIAT OBAT DAN UPAYA PERBANYAKANNYA Purwantoro, R. Subekti; Siregar, Hartutiningsih-M.; Sudarmono, Sudarmono; Praptiwi, Praptiwi
Buletin Kebun Raya Vol 13, No 2 (2010): Buletin Kebun Raya Vol. 13 (2) July 2010
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Lasianthus, a member of the family Rubiaceae has potential for medicinal plants. The aims of the research were to find out the potential antibacterial activity of Lasianthus leaf extract and to investigate the effectiveness of plant propagation by stems cutting. The species tested were Lasianthus laevigatus Blume, L furcatus (Miq.) Bremek and L. cf. obscurus Blume. The extracted leaves in n-hexana, ethyl acetate and methanol were tested in vitro against Staphylococcus aureus and Escherichia coil activities that grown on medium of Mueller-Hinton agar. The methanol extracts of each plant material showed significant antibacterial activities on S. aureus and E. coll. Antibacterial activity increased with increasing concentration of the extracts. Plant propagation by stems cutting were done as a first step of conservation effort for respective species. Stems cutting of L. laevigatus, L. purpureus, L. furcatus, L. acuminatus, L rhinocerotis. and L. cf. obscurus with length of 10 cm were applied with Rotoon F to stimulate root growth. The result of plant propagation research showed that only the stems cutting of L. cf. obscurus grew well although roots did not grow until 4 months.
EFEKTIVITAS METODE KOOPERATIF TIPE GI DAN STAD DITINJAU DARI KEMAMPUAN AWAL Praptiwi, Praptiwi; Handhika, Jeffry
Jurnal Penelitian Pembelajaran Fisika Vol 3, No 1/april (2012): JP2F
Publisher : Jurnal Penelitian Pembelajaran Fisika

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Penelitian ini bertujuan untuk mengetahui: 1) Perbedaan penggunaanmetode pembelajaran kooperatif tipe GI dan STAD terhadap prestasibelajar fisika. 2) Perbedaan kemampuan awal tinggi dan kemampuanawal rendah terhadap prestasi belajar fisika. 3) Interaksi antarametode pembelajaran dan kemampuan awal siswa terhadap prestasibelajar fisika. Jenis penelitian ini adalah eksperimen. Populasi dalampenelitian ini adalah semua siswa SMPN 1 Barat. Sebagai sampeldiambil dua kelas sebanyak 68 siswa yang kemudian dijadikan kelaseksperimen I dan kelas eksperimen II. Kelas VIIIB sebanyak 34 siswasebagai kelas eksperimen I diberikan pendekatan menggunakanmetode pembelajaran kooperatif tipe GI sedangkan kelas VIIIDsebanyak 34 siswa sebagai kelas eksperimen II diberikan pendekatanmenggunakan metode pembelajaran kooperatif tipe STAD denganteknik pengambilan sampel cluster random sampling. Teknik analisisdata yang digunakan adalah analisis variansi (anova) dua jalan.Hasilpenelitian dengan α = 5% dapat disimpulkan bahwa: (1) Metodekooperatif tipe GI lebih baik daripada metode kooperatif tipe STADdengan Fhitung = 92,77. (2) Siswa dengan kemampuan awal tinggimempunyai prestasi belajar fisika yang lebih baik dibandingkandengan siswa yang mempunyai kemampuan awal rendah dengan Fhitung = 4,199. (3) Ada interaksi antara metode pembelajaran dankemampuan awal siswa terhadap prestasi belajar fisika (F hitung =5,16), metode GI dan STAD cenderung berinteraksi pada kemampuanawal rendah.Kata kunci : Metode GI, STAD, Kemampuan Awal, Prestasi Belajar
SKRINING BEBERAPA JAMUR ENDOFIT TUMBUHAN DARI PULAU ENGGANO, BENGKULU SEBAGAI ANTIBAKTERI DAN ANTIOKSIDAN [Screening of Plant Endophytic Fungi from Enggano Island, Bengkulu for Antibacterial and Antioxidant Activites] wulansari, dewi; Putra, Aldho Pramana; Ilyas, Muhammad; Praptiwi, Praptiwi; Fathoni, Ahmad; Palupi, Kartika Dyah; Agusta, Andria
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

Fungal endophytes have been known as sources for bioactives with high chemical structure variability. This study aimed to screen extracts of some endophytic fungi associated with plants from Enggano Island as antioxidant and antibacterial using thin layer chromatography (TLC) bioautography method. Antibacterial ativity was performed against Escherichia coli and Staphylococcus aureus. Seventeen out of 22 extracts showed growth inhibition activity against both bacteria, while 16 extracts were active as DPPH free radical scavenging agents. Further determination on IC50 value of four prominent extracts revealed that fungal endophytes AK3018-1, FC-1, KC-4, and SO-3 have IC50 value of 85, 84, 704, and 347 ?g/ml, respectively in DPPH radical scavenging method. Extracts of fungal endophytes CR-3, CS-2, and SM-2 showed prominent antibacterial activity among other extracts, indicated by wide clear white zone around the spot. Further evaluation on minimum inhibitory concentration (MIC) of those three extracts by microdilution method showed that CR-3, CS-2, and SM-2 have MICs value of 512, >512, and 64µg/ml, respectively against S. aureus.Fungal endophytes AK3018-1, FC-1, KC-4, SO-3, CR-3, CS-2, and SM-2 were respectively isolated from Dioscorea bulbifera tuber, Fibraurea chloroleuca twig, Knema cinerea twig, Smilax odoratissima stem, Cryptocarya sp. twig, Calophyllum soulattri twig, and Smilaxmacrophylla stem.
KOMPONEN KIMIA DAN UJIANTIBAKTERIMINYAK ATSIRIDAUN KI CENGKEH (Urophyllum arboreum (Reinw. ex. Bl.) Korth.) Praptiwi, Praptiwi; Jamal, Yuliasri; Murningsih, Tri
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.206 KB) | DOI: 10.14203/beritabiologi.v6i3.1218

Abstract

This study was done to determine the chemical compounds and antibacterial activity of ki cengkeh (Urophyllum arboreum (Reinw.ex. Bl.) Korth.) essential oil. Ki cengkeh has already known as medicinal plant in some places in Indonesia. This might be correlated with its chemical compounds. The analysis of chemical compounds of ki cengkeh essential oil was done by GC-MS, while antibacterial test was done by paper disc method against 3 bacteria isolates (Staphylococcus aureus, S. epidermidis and Eschericia coli). The result of GC-MS analysis showed that there were 25 compounds with the concentration more than 1% and carryophylen oxide as the highest compound (12.03%). The highest antibacterial activity was found against S. epidermidis.
PENAPISAN FITOKIMA DAN BILANGAN PEROKSIDA (POV) TIGA JENIS TUMBUHAN FAMILI MORACEACE (Artocarpus sp., A.elasticus, dan Ficus sp.) DARI TAMAN NASIONAL BOGANI NANI WARTABONE (SULUT) Harapini, Mindarti; Praptiwi, Praptiwi
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3804.176 KB) | DOI: 10.14203/beritabiologi.v6i4.3456

Abstract

The  aim of study were to determine the chemical compounds and peroxide values (POV) of methanol extract of three plants species belongs to Moraceae family (Artocarpus (Artocarpus sp., A.elasticus, dan Ficus sp.). Their chemical compounds were identified by Cuiley method, while peroxide value was done by iodometri titration. Methanol extract percentage of Artocarpus sp., A.elasticus, dan Ficus sp. were 20.80%, 3.10% and 4.10% respectively. All of the extract tested contained essential oil, sterol and triterpenoid, aglicon flavonoid, tannin, flavonoid glicoside and saponin. POV of Artocarpus sp., A.elasticus, dan Ficus sp. were 325.24, 306.25 and 1580.07 respectively and POV of q tocopherol was 363.96. So, it can be concluded taht all of three methanol extract act as reductor, because their POV less that POV of q tocopherol and might be act antioxidant. These correalted with their chemical compounds such as flavonoid, tannin and peroxide sugar.
Manfaat buah kurma Praptiwi, Praptiwi; Arti, Dwi Windu Kinanti
Jurnal Berkala Ilmiah Kedokteran dan Kesehatan Vol 1, No 4 (2017): MAGNA MEDICA
Publisher : Jurnal Berkala Ilmiah Kedokteran dan Kesehatan

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Latar belakang: Makanan manis, berhubungan dengan kariogenitasnya. Sukrosa merupakan pemanis paling kariogenik dan terbanyak dikonsumsi. Buah kurma banyak mengandung sukrosa, fruktosa, danglukosa alami. Kandungan tanin dan magnesium di dalam kurma bersifat anti infeksi dan anti inflamasi.Metode: Penelitian analitik laboratorik dengan rancangan eksperimental ini bertujuan mengetahui beda pH saliva pasca konsumsi sukrosa, fruktosa, dan kurma. Pelaksanaan penelitian di FKG  dan LaboratoriumTerpadu Unimus tahun 2016 melibatkan 18 mahasiswa dengan karies ≤ 2. Diukur pH saliva awal setelah berpuasa ½ jam. Selanjutnya berturut-turut pH pasca kumur air keran, pasca konsumsi 1 g sukrosa, 1 g fruktosa, dan 1 butir kurma. Analisis data menggunakan uji t dependent dan one way Anova.Hasil: pH saliva rerata dan Simpang Baku berturut-turut dari awal, pasca kumur air keran, pasca konsumsi sukrosa, fruktosa, dan kurma sebagai berikut 6,92, SB 0,217; 6,91, SB 0,151; 6,83, SB 0,229;  6,90, SB0,156;  7,043, SB 0,086. Tidak terdapat beda bermakna pH saliva awal dengan pH pasca kumur air keran. Terdapat beda bermakna pH saliva pasca konsumsi sukrosa, fruktosa, dan kurma ( nilai p = 0,002; p <0,05).Simpulan: pH saliva pasca konsumsi sukrosa dan fruktosa lebih rendah dibanding kurma. Sukrosa dan fruktosa lebih asam sehingga lebih kariogenik dibanding kurma.Kata kunci : pH saliva, pasca konsumsi sukrosa, fruktosa, kurma.
GC/MS PROFILING AND EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF BAYUR (Pterospermum javanicum) BARK EXTRACT Praptiwi, Praptiwi; Fathoni, Ahmad
Teknologi Indonesia Vol 40, No 1 (2017)
Publisher : LIPI Press

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This study aims to identify the chemical compounds of ethyl acetate extract of the bark of bayur (Pterospermum javanicum) and to evaluate its antibacterial activity and potential antioxidant. The estimation of total flavonoid was done by spectrophotometry method. The chemical compounds were analyzed by gas chromatography-mass spectrometry (GC-MS). The antibacterial assay against Staphylococcus aureus Ina-CC B5 and Escherichia coli Ina-CC B4 was done by TLC-bioautographic method, whereas the potential antioxidant was determined by DPPH radical scavenging assay. Meanwhile, its minimum inhibitory concentration (MIC) value and IC50 of the extract were done on microplate 96 well by serial microdilution method. GC/MS analysis revealed the presence of 31 identified chemical compounds in the extract with total flavonoid was 625 mg CE/gram extract. The MIC values against E.coli Ina-CC B5 and S. aureus Ina-CC B4 were >256 ug/ml. The IC50 from DPPH radical scavenging assay was 48.92 ppm indicating that ethyl acetate extract of the bark of bayur is potent antioxidant.
Pengaruh Pemberian Ekstrak Kulit Batang Ailanthus altissima terhadap Perubahan Sel Hati dan Ginjal Mencit Praptiwi, Praptiwi; Kuncari, Emma Sri; Agungpriyono, Dewi Ratih; Chairunnisa, Chairunnisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.777 KB) | DOI: 10.24002/biota.v15i1.2654

Abstract

The aim of this study was to determine the toxicopathological effect of Ailanthus altissima extract in liver and kidney of the mouse. The treatments in this study were: (1) negative control (carboxy methocellulose), extracts with concentration of (2) 1 mg/kg BW, (3) 10 mg/kg BW, (4) 100 mg/kg BW, and (5) 1000 mg/kg BW. Every treatment consisted of 5 mice. After receiving A.altissima extract for seven day consecutively, the mice were then euthanized with overdose of ether. The liver and kidney were taken and then fixed within 10% solution of Buffer Neutral Formalin (BNF). Histopathology slides of liver and kidney were processed and stained with Hematoxylline Eosin. The cell degeneration and cell death were observed with video photo microscope. The lesion of cell death and degeneration of hepatocytes and epithelium of kidney proximal tubule were counted. The data were analyzed statistically with ANOVA test continued with Duncan test. The result showed that cell necrosis of hepatocytes was not significantly different (P>0.05) compared to control treatment, while cell necrosis was increasing in the treatment of ≥ 10 mg/kg BW in tubuli of the kidney.
Aktivitas Antibakteri Ekstrak Metanol Buah Mbosi (Dysoxylum gaudichandianum (A.Juss) Miq.) dan Penapisan Senyawa Kimianya Praptiwi, Praptiwi; Harapini, Mindarti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.42 KB) | DOI: 10.24002/biota.v10i2.2844

Abstract

Mbosi (Dysoxylum gaudichandianum (A.Juss) Miq.) belongs to Meliaceae family, Its potential bioactivity had not been known although the other plants from the same family had been known as natural insectiside or medicinal plants. Phytochemical screening showed that mbosi fruit contained fat, fatty acid, sterol, triterpenoide, tannin, peroxide sugar, alkaloid, steroid glycoside and flavonoid. Identification of mbosi fruit chemical compounds by GC-MS showed that it contained 16 compounds. Compounds with the concentration more than 5 % were butyl cellosolve (11.1%), erythritol (8.48%), glycerol (10.32%), hexadecanioc acid palmitinic ester (16.2%), oleic acid (65.3%) and ethyl oleat (6.2%).  In-vitro antibacterial test on innoculated Mueller Hinton Agar to seven bacteria isolates (Salmonella typhimurium, S. typhii, Eschechiria coli ATCC 25923, Pseudomonas sp., Staphylococcus aureus ATCC 25922, S. epidermidis, and Bacilus subtilis)  showed that 20% methanol extract  inhibit only the growth of  Staphylococcus aureus.
Co-Authors Ahmad Fathoni ANDRIA AGUSTA Arif Nurkanto Arti, Dwi Windu Kinanti Chairunnisa Chairunnisa Dewi Ratih Agungpriyono DEWI WULANSARI Efendy, Oscar Emma Sri Kuncari Hartutiningsih-M. Siregar Jeffry Handhika Kartika Dyah Palupi Mindarti Harapini Mohammad Fathi Royyani, Mohammad Fathi Muhammad Ilyas Priyono, Sumarnie Putra, Aldho Pramana R. Subekti Purwantoro SUDARMONO SUDARMONO Tri Murningsih, Tri Yuliasri Jamal