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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
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Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 42 Documents

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Search results for , issue "Vol 15, No 1 (2010): February 2010" : 42 Documents clear

Pengaruh Tepung Kedelai Kaya Isoflavon terhadap Testosteron Serum, Jumlah Sel Leydig dan Jumlah Sel Spermatogenik pada Tubuli Seminiferi Testis Tikus (Rattus norvegicus) Astuti, Sussi; Sutyarso, Sutyarso
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

The objective of this experiment was to evaluate the effects of isoflavone-riched soybean flour with different levels of isoflavone on testosterone level, total Leydig cells, and total spermatogenic cells in the seminiferous tubules of male rats. Diet was given as isonitrogen and isocaloric with 10% of dietary protein from casein. Twenty five male of Sprague Dawley weaning rats (21 days old) were divided into five groups and treated with isoflavone-riched soybean flour by oral administration with different levels (dosages). The treatment was conducted for 2 months. The treatment of isoflavone-riched soybean flour with higher dosage of isoflavone increased the testosterone levels in the serum and the total Leydig cells in the seminiferous tubules of male rats. The optimum dosage of isoflavone was 1.5 mg/day resulted in the highest total spermatogenic cells of rat testes. The treatment isoflavone-riched soybean flour with 1.5 mg isoflavone/day on male rats resulted in the testosteron level of 2.96Â±0.45 ng/ml, total Leydig cells 70.22Â±9.34; while the number of spermatogonia, spermatocytes, early spermatids, late spermatids, and total spermatogenic cells were 48.44Â±4.82, 60.00Â±3.43, 221.56Â± 16.12, 164.33Â±17.94, and 494.33Â±32.94, respectively.

Identifikasi Hama Penggerek Batang dan Deskripsi Kerusakan pada Tanaman Melina (Gmelina arborea) Triyogo, Ananto; Sumardi, Sumardi; Trisyono, Yohanes Andi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Gmelina arborea is one of the important trees for forest plantation. It is used for pulp, paper and plywood. Considering that G. arborea is an exotic type, this species has highly risk of receiving pest and diseases. This research was aimed to study the biological characteristics, attack pattern and distribution of stem borers. Studies were conducted in PT. Sumalindo (East Kalimantan). The sampling plot was 20x20 m2, and the plants were stratified based on the plant age. Observations were directed to determine the species of stem borers and the damage they caused. Xyleutes ceramica and Acalolepta rusticatrix were documented attacking G. arborea, with their damage intensity of 13% and 54.8%. These insect attacked 22% and 64.4% of the plant plantation.

Pengaruh Pemberian Ekstrak Kulit Batang Ailanthus altissima terhadap Perubahan Sel Hati dan Ginjal Mencit Praptiwi, Praptiwi; Kuncari, Emma Sri; Agungpriyono, Dewi Ratih; Chairunnisa, Chairunnisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

The aim of this study was to determine the toxicopathological effect of Ailanthus altissima extract in liver and kidney of the mouse. The treatments in this study were: (1) negative control (carboxy methocellulose), extracts with concentration of (2) 1 mg/kg BW, (3) 10 mg/kg BW, (4) 100 mg/kg BW, and (5) 1000 mg/kg BW. Every treatment consisted of 5 mice. After receiving A.altissima extract for seven day consecutively, the mice were then euthanized with overdose of ether. The liver and kidney were taken and then fixed within 10% solution of Buffer Neutral Formalin (BNF). Histopathology slides of liver and kidney were processed and stained with Hematoxylline Eosin. The cell degeneration and cell death were observed with video photo microscope. The lesion of cell death and degeneration of hepatocytes and epithelium of kidney proximal tubule were counted. The data were analyzed statistically with ANOVA test continued with Duncan test. The result showed that cell necrosis of hepatocytes was not significantly different (P>0.05) compared to control treatment, while cell necrosis was increasing in the treatment of â‰¥ 10 mg/kg BW in tubuli of the kidney.

Effect of AMF Inoculation on the Growth of Combretocarpus rotundatus (Miq) on a Peat Soil from Central Kalimantan (For Restoration Ex-Mega Rice Project Central Kalimantan) Burhanuddin, Burhanuddin; Kabirun, Siti; Radjagukguk, Bostang; Sumardi, Sumardi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Diperlukan teknik silvikultur dengan memanfaatkan jamur mikoriza arbuskula (JMA) untuk memulihkan hutan rawa gambut yang terdegradasi. JMA berperan penting pada pertumbuhan dan ketahanan hidup pohon-pohonan. Penelitian ini bertujuan untuk mengetahui keberadaan dan bentuk dari JMA di hutan rawa gambut yang sudah terdegradasi pada blok C bekas pengembangan lahan gambut satu juta ha di Kelampangan Kalimantan Tengah. Kondisi lahan hanya ditumbuhi vegetasi jenis-jenis pioner. Contoh tanah diambil dibawah perakaran lima jenis tanaman pioner (Melastoma sp, Combretocarpus sp, Acacia sp, Cratoxylon sp, dan Nephrolepsis sp), kemudian contoh tanah dihitung jumlah spora dengan metode saringan basah. Jumlah spora terbanyak ada di bawah perakaran tanaman Melastoma sp, kemudian Acacia sp, dan Combretocarpus sp. Didapatkan tiga genus JMA: Glomus, Gigaspora, dan Acaulospora. Penelitian di rumah kaca bertujuan untuk mengetahui pengaruh inokulasi JMA jenis Glomus sp TD15, Glomus sp D32, dan Gigaspora sp pada semai perepat (C. rotundatus). Hasil penelitian menunjukkan JMA memberikan pengaruh pada parameter semai yang diukur. Jenis JMA Glomus sp TD15 meningkatkan pertumbuhan semai perepat yang ditunjukkan oleh parameter tinggi semai, diameter semai, dan jumlah daun per tanaman. Pada media gambut semai C. rotundatus sangat tergantung pada asosiasi JMA dalam meningkatkan pertumbuhan tanaman.

Sistematik Filogenetik Pseudomonas Strain Indigenous Pendegradasi Liniar Alkilbenzen Sulfonat Suharjono, Suharjono; Sembiring, Langkah; Subagja, Yusup; Widayati, Wiwik E.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Linear Alkylbenzene Sulphonate (LAS) was the dominant pollutant in the river ecosystem. Indigenous strains of Pseudomonas in river ecosystem had highly potency to LAS degradation. This research was carried out to study relationship of indigenous strains of LAS degrading to Pseudomonas strains. Indigenous strains of bacteria of LAS degrading were characterized based on ARDRA (Amplified Ribosomal 16S rDNA Restriction Analysis) and 16S rDNA sequence. Result of the research shows that Pseudomonas strain J and R which LAS degrading from detergent polluted river ecosystem based on 16S rDNA sequence, isolate J has 98.37% similarity and it has relationship to P. pseudoalcaligenes LMG 1225T whereas isolate R has 84.86% similarity and related to P. stutzeri phen8.

Aktivitas Makan Monyet Ekor Panjang (Macaca fascicularis) di Bumi Perkemahan Pramuka, Cibubur, Jakarta Farida, Hilda; Perwitasari-Farajallah, Dyah; Tjitrosoedirdjo, Sri Sudarmiyati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Long-tailed macaque (Macaca fascicularis) is one of the non human primates which is widely distributed. Long-tailed macaques live in a group consisted of multimales and multifemales. The group size was determined by predator, food resources, and foraging efficiency. Bumi Perkemahan Pramuka Cibubur is a habitat for long-tailed macaques. This place is a camp area and tourism site, which is predominated by akasia (Acacia auriculiformis). This research is aimed to study feeding activity of long-tailed macaque in Bumi Perkemahan Pramuka, Cibubur, Jakarta. Methods used in this research were habituation, census, ad libitum sampling, focal animal sampling, and scan sampling. The identification of food items was carried out by means of direct observation, interview, specimen collection, and identification of plant species. Feeding activities of long-tailed macaques constituted 9.01% of the total daily activities. Food consumed consists of natural and non natural food. Natural food items were leaf buds of awi tali (Gigantochloa apus) 34.76%, jukut pait (Axonopus compressus) 22.61%, and fig (Ficus benjamina) 7.15%, respectively. Furthermore, non natural food items were food leftovers of visitors 29.00% and peanuts 15.52%, respectively. In general, natural food (74.02%) was consumed more frequent than those of non natural food (25.98%).

Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Sub-unit 2 Mitokondria Widayanti, Rini; Handayani, Niken Satuti; Budiarsa, I. Made
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Tarsius is an endemic species in Indonesia that is endangered. In-situ and ex-situ conservation of this species would yield better results if this genetic make up and diversity is determined. The objective of this ressearch was to study the specific genetic marker on COX2 gene of Tarsius sp. Sequencing of PCR product using primer COX2F and COX2R resulted in base sequence of 513 nts. Results of COX2 fragments sequencing were put on multiple alignment with other primates from Genbank with aid of software Clustal W, and were analyzed using MEGA program version 4.1. Eight different amino acid sites were found (amino acid no. 5, 6, 13, 14, 30, 35, 44 and 168). The genetic distance based on nucleotide COX2 calculated using Kimura 2-parameter model indicated that in the smallest genetic distance 0%, biggest 6.8% and average 2.3%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide and amino acid COX2 reveded differentiation among Tarsius from Lampung and Tarsius from Sulawesi, but could not be used to differentiate among T. dianae (from Central Sulawesi) and T. spectrum (from North Sulawesi).

Perubahan Luas Penutupan Padang Lamun Di Kepulauan Seribu, DKI Jakarta Kawaroe, Mujizat; Jaya, Indra; S., Indarto H.; Sari, Dahlia Wulan; W., Sundari Wening
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Seagrass is one of productive and economical marine ecosystem, but recently there are indications that this area is decreasing and every kind of damage is happening. The research was conducted to reveal the change of seagrass width area and to determine the suitable location for donor and acceptor of seagrass transplantation. Methods of research were collecting and analyzing the satellite image of the research location, bathymetri mapping and water quality parameter, and also determining environment charasteristics that suitable to be performed by counting Seagrass Transplantation Suitability Index (STSI). The results show that in year 1999-2004, the derivation area of seagrass was 678.300 m2. Pari island has depth range between 0-50 metres and the physics and chemical parameter of water have a good range where the seagrass can live. According to the analysis result of STSI, suitable areas for seagrass transplantation donor is station no. 5 and 6 for seagrass species of Enhalus acoroides and station no. 2 and 7 for seagrass species Thalassia hemprichii. The implication of this research is that the seagrass needs rehabilitation to overcome seagrass ecosystem for better condition.

Analisis Keragaman Genetik Dyera costulata (Miq) Hook.f. Berdasarkan Marka â€œRandom Amplified Polymorphic DNAâ€ Poerba, Yuyu Suryasari; Widjaya, Elizabeth A.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Dyera costulata (Miq.) Hook.f (Apocynaceae) is a large tree of the lowland tropical rain forest of Southeast Asia that occurs in Thailand, the Malay Peninsula and on the islands of Sumatera and Borneo. Its economic value was in its latex, used as gum chile in the manifacture of chewing gum. Nowadays the timber of this species is largely utilized for the manufacture of pencils and picture frames. The information on genetic diversity of the species is very limited. Hence studies were initiated and genetic diversity were estimated using Random Amplified Polymorphic (RAPD) markers in 47 accessions of Dyera costulata procured from different geographical regions of Jambi. Four selected Operon primers (10 mer) generated a total of 90 consistent amplification products ranging from 150 bp to 2.8 Kb. The cluster analysis showed that the 47 individuals were separated into one main cluster and one individual. The range of genetic dissimilarity value among samples was from 0.06 to 0.71, while genetic distance among populations was from 0.17 to 0.42. These values showed that those 47 accessions of D. costulata from Jambi was genetically originated from diverse population.

PENGARUH PEMBERIAN ANGKAK MONASCUS PURPUREUS TERHADAP KUALITAS MIKROBIOLOGIS FESES TIKUS PUTIH HIPERLIPIDEMIA Yulinery, T; Hardiningsih, R.; Nurhidayat, N.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Angkak is defined as rice fermented by Monascus purpureus, a lovastatin and pigmen producer. The objective of this research was to observe the effect of angkak powder to the digestion system represented by the quality of faeces. The in vivo bioassay was carried out using 20 Sprague-Dawley strain mice into 5 groups. First group was given angkak respectively 0.01g, 0.1g, 0.5g, and two groups of control (positive and negative). The faeces were examined each week for a month. The number or erytrosit, bacteria, and fungus were examined microscopically. The results showed that 0,01g, 0,1g, 0,5g of angkak did not significantly affect of bacteria, fungus and erythrocyte on faeces. The data suggest that the administration of angkak might be useful and safe for the hypercholestrolemic treatment.

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Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

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+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA