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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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INDONESIA
Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 15 Documents
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Search results for , issue "Vol 8 No 2 (2020)" : 15 Documents clear
The Digital Herbarium: Solutions for Data Collection and Identification of Indonesian Plant Diversity Endro Setiawan; Dedy Darnaedi; Ismail Rachman; Teguh Triono; Campbell O. Webb
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.15697

Abstract

Indonesia is one of the world’s biodiversity hotspots. It is estimated to be the home of 9.5% flowering plant species, making it the seventh country with the highest biodiversity. Plant data collection is necessary to ascertain the level of plant biodiversity, as such data help in conservation efforts and long-term management. One of the methods applied is the collection of plants, with the purpose to acquire as much data about its biological resources. The collected specimen are then gathered and processed into a herbarium to be used as an information source in managing biological resources. Unfortunately, there are some difficulties related to the making and management of a herbarium. This study aims to assess the advantages and disadvantages of photo-specimens (digital herbarium) for documenting plant biodiversity in Indonesia. The methods need steps including biological recording, specimen preparing, macro-mode capturing, and last stage identification. About 2149 plants have been gathered from Borneo, Seram, Waigeo, Flores and Sulawesi which consisted of 152 family, 512 genus, and 1,832 species, with a total of 30391 pictures of plant parts. From the experiment conducted on 672 specimens, it achieved 98.8 % accuracy on the family level and 80.1 % accuracy on the genus level, while the species level reached 78.8%. The results showed that digital herbarium can be used to conduct identification and data collection of plant biodiversity. Furthermore, this method is simple, cheap and relatively easier to conduct. The output is a catalog of plant species in specific areas, which provides better understanding about plant identification and biodiversity, enhances conservation practices, and provides better long-term protection for Indonesian plant biodiversity.
Chromium(VI) Heavy Metal Biosorption in Citarum River Water Sample Using Saccharomyces cerevisiae and Rhizopus oryzae Biomass Suci Rizki Nurul Aeni; Ni'matul Murtafi'ah; Handarini Handarini
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.12274

Abstract

The Citarum River was regarded as the World’s dirtiest river in 2018, characterized by a Basin situated adjacent to several textile and electroplating industries. Hexavalent chromium metal (Cr6+) is a toxic, carcinogenic heavy metal found in the wastes of these industries. Furthermore, biosorption with biological adsorbents, including Saccharomyces cerevisiae and Rhizopus oryzae, is an alternative method for treating water polluted with heavy metals. This study therefore aims to adsorb Cr6 + heavy metals from Citarum River water using S. cerevisiae and R. oryzae biomass in two locations: Nanjung and Pangauban, with various biomass concentration and stirring speeds. For this study, 0.25, 0.5, and 0.75 g of R. oryzae suspension, as well as 250, 500, and 750 µ of S. cerevisiae were used as adsorbent, while rotated at speeds of 250 rpm, 750 rpm and 1500 rpm. Subsequently, the Cr6 + metal content was measured using a UV-Vis spectrophotometer at a wavelength of 525 nm, and calculated based on a standard curve. The results showed S. cerevisiae and R. oryzae are able to reduce the levels of Cr6+ in Citarum river water. The most reduction was obtained with the highest concentration of the adsorbents, 750 µ for S. cerevisiae and 0.75 g for R. oryzae, at the speed of 1500 rpm. S. cerevisiae and R. oryzae have great potential as biosorbents for the in situ remediation of Citarum River contaminated with heavy metals.
Quantitative Analysis of Floristic Composition, Biological Spectrum and Leaf Spectrum of a Sacred Grove in Jhargram District, West Bengal, India Uday Kumar Sen; Ram Kumar Bhakat
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.16407

Abstract

Sacred Groves are tracts of virgin forests, left untouched and protected by local people, because of culture and religious beliefs. These tracts are remnants of the once-dominant flora, reservoirs of the rich biodiversity, and the last refuge for preserving the rich indigenous culture and traditions. For these reasons, the biological and leaf spectra, as well as the conservation status of the current sacred grove vegetation, Maa Mongalmoyee Than (MMT) in Jhargram district of West Bengal, India, have been studied. Data were collected during different seasons. The floristic list is taxonomically arranged based on clade, order, and family. In addition, photographs of some common, locally uncommon, endemic and valuable plant species within the sacred grove were taken. The herbarium sheets were then described by matching properly annotated materials available at the Herbarium Section of Vidyasagar University as well as the Botanical Survey of India. The results of floristic studies showed 217 MMT's angiosperm species, belonging to 196 genera, distributed under 59 families of 27 orders. Furthermore, Poales (13.82%) and Fabaceae (12.44%) are the dominant order and family, respectively, in terms of species population. Meanwhile, the biological spectrum showed the grove enjoys a "thero-chamaephyte" phytoclimate form, as well as a comparatively undisturbed status, being a sacred grove. Also, the preservation of germplasm within the grove is based on traditional belief in the social system.
The Potential Role of 6-gingerol and 6-shogaol as ACE Inhibitors in Silico Study Yohanes Bare; Maria Helvina; Gabriella Chandrakirana Krisnamurti; Mansur S
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.15704

Abstract

Hypertension has become the third highest cause of death in Indonesia. The condition is correlated with angiotensin-converting enzyme (ACE), and possibly managed with the use of drugs. In addition, some natural compounds, including 6-shogaol and 6-gingerol from ginger, are used to decrease blood pressure. However, the mechanism and binding site of these compounds to ACE protein is currently unclear. This study, therefore, aims to investigate the potential role of these compounds as an angiotensin-converting enzyme inhibitor. The ACE protein was downloaded from Protein Data Bank (PDB) database with the ID: 3bkk, while the 6-shogaol (CID: 5281794) and 6-gingerol (CID: 44559528) ligands were obtained from the PubChem database. Meanwhile, molecular docking was established using HEX 8.0.0 software. The analysis examined the amino acid residues and the bonds formed from these interactions. According to the results, 14 amino acid residues were formed by the interaction between 6-shogaol and ACE, while the interaction between 6-gingerol and ACE formed eight amino acids. Also, 13 amino acid residues in the novelty binding site of ACE were discovered to be blocked by the ligands from ginger. Therefore, the compounds have potential roles as inhibitors, and this possibly helps to prevent regulation of the renin-angiotensin system. These interactions also formed hydrogen bonds, as well as electrostatic, unfavorable, and hydrophobic sites, making the binding stronger than others. 
Phytochemical Screening and Larvicidal Evaluation of Phyto-synthesized Silver Nanoparticles using Palmyra Palm Sprout Extract Wilson Lamayi Danbature; Zaccheus Shehu; Muhammad Mustapha Adam; Ahmed Mohammed Bello
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.17581

Abstract

One of the objectives of nanotechnology is to synthesize effective nanoinsecticides in a bid to reduce the prevalence of the numerous diseases caused by mosquitoes. This synthesis is even more vital in cases where phytochemicals from plant extracts are used as reducing agents. This study aims to determine the phytochemicals present in Palmyra (Borassus aethiopum) sprout extract, perform green synthesis of silver NPs with the sprout extract, and spectroscopic investigation using FT-IR and UV-Visible spectrophotometers, in addition to the main aim of evaluating the AgNPs’ applications as a nano-larvicide. For this study, a total of ten phytochemical analyses was performed. Subsequently, the sprout extract was used as a reducing agent in the synthesis of silver nanoparticles (AgNPs). Characterization with Fourier transform infrared and Ultraviolet-visible spectrometry was then carried out to confirm the synthesis. In addition, the AgNPs were further analyzed for larvicidal potency against 1st, 2nd, 3rd and 4th instars Culex quinquefasciatus mosquito larvae, at interval concentrations of 5, 10, 20, 25, and 50 ppm. The results confirmed the presence of alkaloids, flavonoids, saponins, coumarins, glycosides, tannins, phenols as well as quinines, however, sterols, steroids, and terpenoids were not detected. The LC50 and LC90 values discovered to be 9.103 ppm and 134.463 ppm with a correlation of 0.815, as well as 10.316 ppm and 118.810 ppm with a correlation of 0.801, for the 1st and 2nd instars, respectively. Meanwhile, the 3rd and 4th instars had LC50 and LC90 values of 17.585 ppm and 179.052 ppm, respectively, with a correlation of 0.807. This study provides a basis for extracting and analyzing the reduction potential of the phytochemicals present in the sprout extract, as well as the application of AgNPs, in controlling the mosquito larvae population.
Estimation of Several Commercial, Phenotypic and Reproductive Traits' Performance, Using the Quantitative Genetic Method for Kamper Chicken Line I Wayan Swarautama Mahardhika; Abiyyayumna Rif’at Chasnaurosyiqoh; Joseph Chohansandhika; Feren Putri Sholiha; Nurachmad Bagas Indriarto; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.15648

Abstract

In this study the genetic resource of Pelung chicken from Cianjur, West Java, Indonesia, was exploited. Pelung chicken has a higher body weight growth, unique meat flavor, and superior posture, compared with other indigenous breeds. Kamper chicken line selective breeding program was conducted, to increase the performance of Pelung breed by crossing with Layer Lohmann Brown-Classic. The Layer Lohmann Brown-Classic is an imported laying-type breed, which is widely known for its reproductive performance, based on the egg productivity. This study aims to use quantitative genetic method in estimating the commercial and reproductive traits' performance of Kamper chicken line. Based on commercial, phenotypic and reproductive traits, the progenies in Kamper chicken line have significant improvements, compared to the parental cross of Pelung and Layer Lohmann Brown-Classic. The quantitative genetic method was used in describing and underlying some phenomenon, in the selective breeding program. The results showed that the phenotypic and reproductive types of progenies in Kamper chicken, have significant improvements compared to the parental crossing of Pelung breed and Layer Lohmann Brown-Classic. Although quantitative genetic method is utilized in basic breeding program with significant precision and rapidness, it is only used in the preliminary study, for the advanced type. Therefore, the addition of quantitative trait loci (QTL), provide a more thorough genetic examination, and play a role in selective breeding program.
Arrhenius Plot Analysis of the Temperature Effect on the Biodegradation Rate of 2-chloro-4-nitrophenol Mohd Yunus Shukor
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.17419

Abstract

Several models are available to determine the effect of temperature on the growth rate of microorganisms on substrates. An example is Arrhenius model, which is very popular because it has few parameters. For the first time, a discontinuous chevron-like graph of apparent activation energy based on the Arrhenius plot on the growth of 2-chloro-4-nitrophenol by Cupriavidus sp. is reported. The plot of ln mm against 1/T shows a discontinuous chevron-like graph for the entire investigated temperature range with an inflection at 27.75°C. This indicates that the existence of 2 activation energies for growth on 2-chloro-4-nitrophenol ranges from 20 to 40°C. Furthermore, a regression analysis from 20–25°C and 30–40°C results in activation energies of 88.71 kJmol-1 and 75.16kJ mol-1, respectively. This is probably the first time a Chevron-like graph was observed for the Arrhenius plot on the effect of temperature on the growth rate of 2-chloro-4-nitrophenol.
First Provincial Record of Black-crowned Night heron (Nycticorax nycticorax) in West Sumatra and the Summary of Its Sumatran Records Muhammad Nazri Janra; Aadrean Aadrean; Henny Herwina
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.12642

Abstract

The Black-crowned Night heron (Nycticorax nycticorax) is a known visitor in Sumatra with more sighting records in the eastern side of the island. Meanwhile, on the west, it was historically recorded from only two offshore islands, namely Nias and Enggano. During the waterbird survey from early 2018 at Pasir Jambak Beach of Padang City in the coastline of West Sumatra, a Night heron was spotted among the colony of mixed egret species. This study aims to update the knowledge of this species in West Sumatra, as well as its general occurrence in Sumatra. This study was conducted by counting the population of each waterbird species found in the research area, while for documentation purposes, a Nikon Coolpix P900 was used to take pictures. Species identification was guided with related books for the Sumatera area. Data then were analyzed and outlined descriptively in accordance with what accumulated from the field survey and online searching. The result showed that the first sighting of Black-crowned Night-heron in West Sumatra follows the common fly-through route of migrant birds in eastern side of Sumatra. The summary of the compiled records of this species indicates that it is probably a vagrant along the western coastline, while it visits more frequently on the eastern side. This is the first record of its kind in the province of West Sumatra, although, previous records cover almost all provinces in Sumatra. 
Analysis of Amino Acid Sequence of SARS-CoV, SARS-CoV-2, and MERS-CoV Spike Glycoproteins: Preliminary Study for Obtaining Universal Peptide Vaccine Candidates Yani Suryani; Opik Taupiqurrohman
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.15696

Abstract

In the manufacture of universal peptide vaccines, it is necessary to analyze the amino acids of the various candidates. Therefore, this study aims to examine the amino acids of the spike glycoproteins of SARS-CoV, SARS-CoV-2, and MERS CoV. The method used is the alignment of the amino acid spike glycoprotein between SARS-CoV with SARS-CoV-2, MERS CoV with SARS-CoV-2, and SARS-CoV with MERS-CoV using web-based software water emboss. The analysis result showed that SARS and SARS CoV-2 were very similar with 87% similarity and 76.4% identity values. In contrast, SARS CoV-2 with MERS and SARS with MERS were very different, having similarity and identity values less than 70%. Therefore, it is reasonable to conclude that spike glycoprotein's peptide is only useful from attacks by the SARS-CoV and SARS-CoV-2 viruses.
Strategy for Designing the Synthetic Gene Encoding Human papillomavirus Major Capsid L1 Protein for Heterologous Expression in Escherichia coli System Kartika Sari Dewi; Wien Kusharyoto
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.15805

Abstract

DNA is widely used to construct heterologously expressed genes. The adaptation of the codons to the host organism is necessary in order to ensure sufficient production of proteins. The GC content, codon identity and the mRNA from the translation site are also important in the design of the gene construct. This study performed a strategy for the design of synthetic gene encoding HPV52 L1 protein and several analyses at the genetic level to optimize its protein expression in the Escherichia coli BL21(DE3) host. The determination of the codon optimization was performed by collecting 75 HPV52 L1 protein sequences in the NCBI database. Furthermore, all the sequences were analyzed using multiple global alignments by Clustal Omega web server. Once the model was determined, codon optimization was performed using OPTIMIZER and the web server of the IDT codon optimization tool based on the E. coli B. The generated open reading frame (ORF) sequence was analyzed using Restriction mapper web server to choose the restriction site for facilitating the cloning stage, which is adjusted for pJExpress414 expression vector. To maximize the protein expression level, the mRNA secondary structure analysis around the ribosome binding site (rbs) was performed. A slight modification at the 5’-terminal end waa carried out in order to get more accessible rbs and increasing mRNA folding free energy. Finally, the construction of the synthetic gene was confirmed to ensure that no mutation occurs in the protein and to calculate its Codon Adaptation Index (CAI) and GC content. The above strategy, which leads to a good ORF sequence with the value of the free mRNA folding energy around rbs, is -5.5 kcal / mol, CAI = 0.787 and GC content 49.5%. This result is much better than its original gene. This result is much better compared to its native gene. Theoretically it is possible that this synthetic gene construct generates a high level protein expression in E. coli BL21 (DE3) under the regulation of the T7 promoter.

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