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Media Kedokteran Hewan
Published by Universitas Airlangga
ISSN : 02158930     EISSN : 2775975X     DOI : 10.20473/mkh.v32i1.2021.1-11
Core Subject : Health,
Medicine & Pharmacology Veterinary
Media Kedokteran Hewan (p-ISSN: 0215-8930) (e-ISSN: 2775-975X) (established 1985) publishes all aspects of veterinary science and its related subjects. Media Kedokteran Hewan publishes periodically three times a year (January, May, and September). Media Kedokteran Hewan publishes original articles, review articles, and case studies in Indonesian or English, with an emphasis on novel information of excellent scientific and/or clinical quality, relevant to domestic animal species and biotechnology of veterinary medicine from researchers, lecturers, students, and other practitioners around Indonesia and worldwide.
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Articles 7 Documents
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Search results for , issue "Vol. 33 No. 3 (2022): Media Kedokteran Hewan" : 7 Documents clear
Pathology and Characterization of Fowlpox Virus Infection in a Turkey-Chicken Backyard Flock, Nigeria Olatunde Babatunde Akanbi
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.177-187

Abstract

Cutaneous scabs were seen on the nares and shanks in three grower turkeys in a mixed turkey-chicken flock in Langtang-north LGA of Plateau State, Nigeria. A chicken in the flock had diphtheritic membrane covering the mucous membranes of the oro-pharynx and the turkeys had cutaneous pox lesions on the nares and shank. Fowlpox virus infection was diagnosed by gross and histopathology and confirmed by isolation of the virus in chorioallantoic membrane of 9-12 weeks chicken embryonating eggs. The DNA of Fowl Pox Virus (FPV) was detected in the cutaneous scabs of the turkey and chicken using Polymerase Chain Reaction (PCR) which amplified a 578 base pairs fragment of the 4b core protein gene. Sequencing and phylogenetic analysis revealed that the fowlpox virus responsible for this infection clustered with the sublineage A1 genotype of clade A of fowlpox virus and shares 98% homology with the vaccine strain produced in Nigeria. The sequence has been deposited in the GenBank under the accession number MK435242. Our results confirmed the presence of FPV in the turkeys and suggest a predisposition by the chickens. We therefore suggest vaccination for small holder poultry to mitigate against mortality in turkeys and chickens and molecular epidemiology of fowlpox viruses in Nigeria to unravel viral evolution.
Laporan Pertama Kasus Infestasi Spinturnix spp. pada Kelelawar Pemakan Buah (Rousettus spp.) Fransiska Okta Zania; Audina Putri Geraldine; Citra Kurnia Putri; Ryanka Edila; Aditya Yudhana
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.233-243

Abstract

Rousettus spp. is one of the wild animals that may transmit various diseases including zoonotic. Rousettus spp. or known as fruit bat is found in Java island and belongs to the Pteropodidae family. Generally, Rousettus spp. consumes fruit and floral products. This case report aims to detect ectoparasites in fruit bats (Rousettus spp.) found at Djawatan Banyuwangi, East Java. The samples in this case report are seven fruit bats (Rousettus spp.). Based on the results of laboratory identification using the whole mount method, from 7 samples of fruit bats (Rousettus spp.), there are five fruit bats (Rousettus spp.) were infected with Spinturnix spp.. Spinturnix spp. that obtained from fruit bats (Rousettus spp.) were examined using a binocular microscope (Olympus CX-23, Tokyo Japan) with a magnification of 100 times. Images of ectoparasites and bats were taken using a Nikon d5300 camera. Total amount of Spinturnix spp. that was successfully obtained from 5 fruit bats (Rousettus spp.) are 25 individuals.
Ergothioneine Modulates Biomarkers of Oxidative Stress, Biochemical Profiles and Rectal Temperatures of Arabian Stallions Following Exercise in A Hot-Humid Environment Adakole Adah; Joseph Ayo; Peter Rekwot; Tagang Aluwong; Deborah Adah
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.163-176

Abstract

These experiments were performed to determine the effect of ergothioneine (EGT) and environmental parameters on some physiological profiles of Arabian stallions following an 1800 m race in a hot-humid environment. Twelve stallions having a mean weight of 401 ± 7.33 kg and aged 5.28 ± 1.14 years were used as subjects. They were divided into two groups of treated stallions (n = 6) and untreated stallions (n = 6). Group I which was the experimental group was administered with EGT (0.5 mg/kg orally)every week for two months while group II which served as controls was not treated. The temperature and the relative humidity of the experimental site were determined for six days and on the day of the experiment. The temperature-humidity index (THI) was also calculated. Vital parameters and some biochemical parameters of all stallions were determined before the commencement, immediately after, and one hour after the exercise. Some biomarkers of oxidative stress and serum biochemical parameters (aspartate aminotransferase, creatinine and lactate dehydrogenase) of the stallions were also determined.The biochemical parameters were higher (P < 0.05) in the untreated group than in the treated group. The results obtained showed that EGT lowered the rectal temperature and modulated biomarkers of oxidative stress and biochemical profiles.
Hubungan Kadar Antioksidan Superoxide Dismutase (SOD) dengan Abnormalitas dalam Tingkat Pengencer dan Lama Simpan Semen Mentog di Suhu Dingin Fitriani
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.208-213

Abstract

Tujuan penelitian ini adalah mengetahui pengaruh penambahan kuning telur sebagai cryoprotectant dalam tingkat pengencer dan lama simpan pada semen mentog yang disimpan pada suhu dingin.Mentog yang digunakan dalam  penelitian ini adalah 4 ekor ternak entog  jantan berumur sekitar 1,5 – 2 tahun dengan berat badan 3 – 3,5 kg.   Penampungan semen mentog dilakukan pagi dengan frekuensi penampungan 2x/minggu, Metode penelitian yang digunakan metode eksperimental dengan Split Plot pola petak terbagi.   Faktor pertama sebagai Petak Utama adalah tingkat pengencer yaitu tanpa pengencer (A0); 5 X(A1);10X(A2) dan 15X (A3),faktor kedua sebagai Anak Petak adalah lama simpan  0(B0); 60 (B1); 120  (B2) dan 180 menit yang disimpan  pada  suhu dingin 4ºC.Tiga kelompok kandang mentog sebagai ulanganyang diambil semennya.   Hasil yang diperoleh melalui analisis ragam menunjukkan terdapat pengaruhtetapi tidak  nyata baik SOD maupun abnormalitas(P<0.05).  Kesimpulan adalahtingkat pengenceran dan lama simpan beda berpengaruh tetapi tidak nyata (P < 0,05).   Kuning telur dalam pengenceransemen  mentog sebagai cryoprotectant dapat mempertahankan semen mentog. 
Escherichia Coli Infections, and Antimicrobial Resistance in Poultry Flocks, in North Central Nigeria Olatunde Babatunde Akanbi; Isaac Dayo Olorunshola; Peter Osilojo; Eunice Ademola; Godwin Agada; Julius Aiyedun; Christiana Ibironke Odita; Shola David Ola-Fadunsin
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.188-207

Abstract

To investigate Eschericiacoli infections in poultry flocks, 291 tissue samples from 237 necropsied carcasses submitted from ninety-nine (99) poultry flocks in north central Nigeria were analysed. These flocks comprised layer chicken, broiler, pullet, cockerel, turkey, quail, guinea fowl and ducks. Tissue samples were pre-enriched in 10 mL buffered peptone water media and aliquots were inoculated into selective enrichment broth, sub-cultured onEosin Methylene Blue (EMB) agar and MacConkey agar (MCA) and colonies of E. coli was examined based on cultural morphological characteristics. Layer poultry and laying quails exhibited reproductive lesions which correlates with history of disruption and reduction in egg production. Antibiotics resistance rate was significant (p <0.01) with macrolide and penicillin classes been the most resistant antibiotics in layers and broiler, while quinolones and aminoglycoside were most significantly susceptible (p <0.01). Multidrug resistance (MDR) was found in 56% of the E. coli isolates, with high prevalence in younger birds.
Studi Filogenetik Gen Penyandi G Studi Filogenetik Gen Penyandi Glikoprotein Virus CyHV-3 Pada Ikan Koi Di Beberapa Daerah Jawa Timur Indra Sukma Putra Alisukma
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.214-232

Abstract

Virus Cyprinid Herpesvirus 3 (CyHV-3) merupakan salah satu penyebab infeksi akut penyebab kematian pada ikan koi. Genom CyHV-3 mengkode lima keluarga gen:ORF2, reseptor tumor necrosis factor (TNFR), ORF22,ORF25, dan RING. Dalam pemantauan Balai Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan Surabaya I tahun 2015 ditemukan penyakit Koi Herpesvirus di beberapa daerah di Jawa Timur yaitu Malang, Madiun, Kediri dan Blitar.  Sampel yang berupa ikan koi dilakukan deteksi terhadap CyHV-3 dengan metode PCR,setelah dipastikan positif terhadap virus CyHV-3, dilakukan PCR kembali pada isolat DNA dengan menggunakan primer spesifik terhadap gen penyandi glikoprotein (ORF25),selanjutnya disekuensing menggunakan metode direct sequencing sehingga diketahui sekuen nukleotida. Sekuen nukleotida selanjutnya diterjemahkan dalam bentuk asam amino. Hasil sekuen nukleotida dan asam amino di alignment menggunakan program GENETYX ver 10, serta dilanjutkan analisa filogenetik. Hasil pohon filogenetik pada gen ORF25 menunjukkan isolat Jawa Timur (Malang, Madiun, Kediri dan Blitar,) dekat kekerabatannya dengan isolat China (KP004892), Jepang (AP008984) dan Korea (JQ308816) daripada dengan isolat Amerika (NC_009127.1) dan Israel (DQ177346). Pada penelitian ini dapat disimpulkan bahwa isolat CyHV-3Jawa Timur (Malang, Madiun, Kediri dan Blitar) memiliki homologi dan tingkat kekerabatan yang tinggi pada sekuen nukleotida dan asam amino dan diindikasikan berasal dari China,  sehingga gen ORF25pada isolat tersebut dapat diketahui untuk mengidentifikasi asal muasal penyakit dan menentukan kebijakan sehingga dapat segera dicegah/dikendalikan penyakit KHV pada ikan koi.
Cytotoxicity of Kembang Bulan (Tithonia diversifolia ) Ethanolic Leaf Extract on Rabbit Limbal Mesenchymal Stem Cells Hani Plumeriastuti; Vida Seanita Zahro; Nusdianto Triakoso; Fedik Abdul Rantam; Iwan Sahrial Hamid; Mustofa Helmi Effendi; Annise Proboningrat
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.233-239

Abstract

Kembang Bulan (Tithonia diversifolia ) is known as an herbal plant that has many useful properties to cure disease, such as diabetes, malaria, and other infections. Instead of much usefulness, T. diversifolia has main biological properties that may induce toxicity in this plant known as sesquiterpene lactones. The aim of this study was to identify the cytotoxicity effect of T. diversifolia leaf extract on limbal mesenchymal stem cells of rabbits using the MTT assay. T. diversifolia leaf extract was divided into 4 concentrations (0.5%, 0.25%, 0.125%, and 0.0625%) and given to limbal mesenchymal stem cells of rabbits in 96-well microplates, which would be incubated for 24 hours. The cytotoxicity result was obtained using the MTT assay method. Every well would be observed to see the cell life level of each concentration. The optical density absorbance was calculated using a microplate reader. T. diversifolia leaf extract with a concentration of 0.0625% has the biggest viability with a level of existence of limbal mesenchymal stem cells of 50%, and the other T. diversifolia leaf extract concentrations of 0.5%, 0.25%, and 0.125% have less than 50% level of existence of limbal mesenchymal stem cells.

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