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Potency of Moringa Leaf Extract (Moringa oleifera, Lam.) in Lowering the fatty infiltration of Heart on Hypercholesterolemia Rat (Rattus norvegicus). Adinugroho, Raden Ismail Hafidh; Suparyanti, Endang Listyaningsih; Muthmainah, Muthmainah
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Introduction: The high consumption of high cholesterol foods associated with increased mortality from cardiovascular disease. Because of the side effects like myopathy and the high price of hipercholestrolemia therapy, public interest for the use of natural medicine increased. Moringa leaves contain flavonoids, polyphenols, and Î²-sitosterol so that it can reduce fatty infiltration at heart. Histological Study of effect moringa leaves on heart has never been performed before. This study aimed to determine whether the extract of Moringa leaf (Moringa oleifera Lam.) can lower the fatty infiltration of heart on hypercholesterolemia rat model (Rattus norvegicus). Methods: This study was an experimental laboratory with post test only control group design. A sample of male rats were consisting of Sprague Dawley rats, weight 150-200 g, aged 2-3 months. The sampling technique used is incidental sampling. Twenty eight of samples were randomly divided into four groups, each group consisted of 7 rats.Group KK is given aquades. Group K (-), K1, and K2 is given a high-fat diet for 42 days. Group K1 and K2 were each given Moringa leaf extract at a dose of 200 and 400 mg pe kg of body weight from day 42nd until day 70th. Termination was performed on 71th day, then their hearts were prepared for specimen stained with hematoxylin eosin. Heart ventricle fat area was observed with 1000x magnification on light microscope. The data were analyzed using Kruskal-wallis comparative test and then Post-hoc test Mann Whitney was performed (Î± = 0.05). Results: The observation area of fat at left and right ventricle in KK, K (-), K1, K2 were 378.81Â±53.32; 1280.15Â±133.94; 386.74Â±57.87; and 326.1Â±29.89 Î¼m. Kruskall-Wallis test showed significant result (p = 0.000). Mann Whitney test results showed no statistically significant difference in area of ventricle fat (p <0.05) among all groups, except between groups of KK and K1 (p = 0.633). Conclusion: The extract of leaves of Moringa (Moringa oleifera Lam.) can reduce steatosis / fatty liver histopathology picture rat heart (Rattus norvegicus) model of hypercholesterolemia. Â Keywords:Moringa leaf extract, heart, fatty infiltration, rat. Â

Comparison of the effect of alkaline water and metformin towards blood glucose level in diabetic model rats Abdurahim, Ghani; Yudhani, Ratih Dewi
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Introduction: Diabetes mellitus is a chronic disease, resulting from defects in insulin secretion, insulin action, or both. Indonesia ranked 7 out of 10 on countries with the most highest number of diabetic patient. Oxidative stress and high blood glucose level were occurring under diabetic condition. High prevalence of diabetes and expensive cost for treatment lead to development of alternative treatment for diabetes. Alkaline water with pH>7 acts as antioxidant and had been proven to lower bloodÂ glucose level via increase in glucose uptake. Metformin is the first line drugs for type 2 diabetes. The aim of this research was to compare the effect of alkaline water and metformin on blood glucose level of diabetic model rats. Methods: This research was experimental laboratory with the randomized pre and post test controlled-group design. This research was using 24 in total of 2 months old male Wistar albino rats weighting 180-200 g. Rats induced by alloxan 155mg/kg bw on the first day, then randomly divided into 4 groups with different treatments. Negative control group (K-) was given aquadest, positive control (K+) metformin 9mg/200g bw, Alkaline water 1 (Alk1) 23 ml, and alkaline water 2 (Alk2) 46 ml of alkaline water. These treatment was given once daily on the 5th until 12th day. Blood glucose level was measured three times: before induced by alloxan, after induced by alloxan, and post-treatment. Data were analyzed using repeated measures ANOVA (Î± = 0.05) followed by Multiple Comparisons LSD (Î± = 0.05).Â Result: The mean of post-treatment blood glucose level on K-, K+, Alk1, Alk2Â respectively (in mg/dl) were 242.33Â±50,06; 67.83Â±12.90; 71.83Â±8,37; 70.83Â±9.72. The result of Repeated Measures Anova showed significant difference on mean blood glucose level (p=0.000). The result of LSD on mean of blood glucose level reduction showed significant difference (p=0.000) between K- and K+, K- and Alk1, K- and Alk2. The results of LSD showed no significant difference between K+Â and Alk1 (p=0.536) as well as K+ and Alk2 (p=0.280).Conclusions: Alkaline water has comparable effect with metformin on blood glucose level of diabetic model rats.Keywords: alkaline water, metformin, blood glucose level, alloxanÂ

Comparison of Hematology Analyzer Cyanmethemoglobin Method and Point-of-Care Testing (POCT) Electrode-Based Biosensor Method in Measurement of Hemoglobin SHIDQI, LISANA; KUSUMAWATI, RATNA; YUDHANI, RATIH DEWI
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Introduction: Measurement of hemoglobin concentration can be done by hematology analyzer (HA) cyanmethemoglobin method (gold standard) and point-of-care testing (POCT) electrode-based biosensor method. POCT electrode-based biosensor method is one of the hemoglobin POCT and has not been studied before in Indonesia. This study examined the difference and correlation hemoglobin concentration by HA cyanmethemoglobin method and POCT electrode-based biosensor method. Methods: This was a cross sectional study in 4 senior high schools of Sukoharjo in Indonesia on Desember, 2016. Senior high schools were selected using a simple random sampling and senior high schools grade selected using a stratified random sampling. Among 173 grade 10 and 11 senior high school girls in this study the capillary and venous blood samples were collected. Capillary blood sample collected was immediately processed to measure the hemoglobin concentration using POCT electrode-based biosensor method and venous blood sample was collected to measure the hemoglobin concentration using HA cyanmethemoglobin method. Statistical analyses used were Mann-Whitney and Spearmanâs correlation coefficient (Î±=0.05). Results: Hemoglobin concentration determined by the POCT electrode-based biosensor method compared to HA cyanmethemoglobin method was significantly different (p=0.000 ) and there was positive moderate correlation (r=0.438; p=0.000). Conclusions: Hemoglobin concentration assessment by POCT electrode-based biosensor method has shown significantly different and positive correlation with HA cyanmethemoglobin method. Keywords: hemoglobin concentration, HA cyanmethemoglobin method, POCT electrode-based biosensor methodÂ

Roxburghine B and OxoxylopinePotential as Hepcidin Antagonist Insilico for Inflammatory Anemia Yotriana, Safirah .; Suselo, Yuliana Heri; ., Muthmainah .
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Introduction: Anemia affects half a billion women in the world and become a problem, especially in pregnant women. One of the cause of anemia is inflammatory disease which also has a high prevalence in Indonesia. Therapies currently used to treat anemia are based on the underlying disease. Anticalin is a protein that works as hepcidin antagonist. Anticalin has completed the clinical phase II, but it has not been distributed to public market yet. Therefore, a new, easily accessible and more effective drug is required. Indonesian herbs have been widely used as medicinal plants in the community and are potential to be developed as drugs. The purpose of this study is to identify Indonesian phytochemicals that can act as hepcidin antagonist. Methods: The research was a bioinformatics study with molecular docking method. Three-dimensional structure of human hepcidin as a target protein and Anticalinas a standar therapy were downloaded from the website of the Protein Data Bank (PDB). The active compounds were obtained from HerbalDB and the three-dimensional structure was from PubChem National Center for Biotechnology Information (NCBI). This study was performing using AutoDock Vina 1.1.2 to analyze the binding affinity. Molecular modification was performed with Autodock Tools 1.5.6. Visualization was done with Chimera 1.10 and PyMol 1.3. Results: The docking scores between hepcidin and Anticalin was -4.6 kcal/mol at Cys13,Cys14, Arg16, and Ser17. The compound Roxburghine B and Oxoxylopine could interact with the ligand binding domain and had the docking scores lower than Anticalin. Conclusions: Roxburghine B and Oxoxylopine are potential to become hepcidin antagonists insilico for inflammatory anemia. Keywords: anemia inflamasi, phytochemical, molecular docking, hepcidin, Anticalin Â

In Vitro Antihelmintic Effect of Mahagoni (Swietenia mahagoni Jacq) Seeds Etanol ExtractAgainst Ascaris suum Goeze Salam, Yusuf Arif; Sari, Yulia; Setyawan, Sigit
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Introduction: Mahagoni seeds contain flavonoid, saponin, and tannin that that have been known having anthelmintic effect. This study aimed to determine the antihelmintic effect of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract against Ascaris suum Goeze in vitro. Â Methods:The study was a laboratory experimental research using post â test only controlled group design. The subject were adult Ascaris suum Goeze worms. The sampling technique used was quota sampling. This research was using mahagoni (Swietenia mahagoni Jazq) seeds etanol extractwith concentration 30% w/v, 50% w/v, 70% w/v, 90% w/v, NaCl 0.9% (negative control), and albendazol 0.5% (positive control).Data were analyzed with Pearson correlation test to determine correlation between concentration of mahagoni (Swietenia mahagoni Jacq) seeds etanol extractÂ and amount of worms death. Probit analysis has been done to find LC50. Results: Pearson correlation testÂ showed significant result p=0.00 and strong correlation r = 0.87. Probit analysis results showed LC50mahagoni (Swietenia mahagoni Jacq) seeds etanol extract was 40.66% w/v. It means that 40,66% concentration of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract can cause the death of half sample. Conclusions:Mahagoni(Swietenia mahagoni Jacq) seeds etanol extract had effects to the mortality of Ascaris suum Goeze in vitro with strong correlation between additional amount of extract concentration and amount of worms with LC50value= 40,66% w/v. Keywords :Antihelminthic, Swietenia mahagoni Jacq, alkaloid, Ascaris suum GoezeÂ

Roxburghine B and OxoxylopinePotential as Hepcidin Antagonist Insilico for Inflammatory Anemia Safirah . Yotriana; Yuliana Heri Suselo; Muthmainah . .
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Introduction: Anemia affects half a billion women in the world and become a problem, especially in pregnant women. One of the cause of anemia is inflammatory disease which also has a high prevalence in Indonesia. Therapies currently used to treat anemia are based on the underlying disease. Anticalin is a protein that works as hepcidin antagonist. Anticalin has completed the clinical phase II, but it has not been distributed to public market yet. Therefore, a new, easily accessible and more effective drug is required. Indonesian herbs have been widely used as medicinal plants in the community and are potential to be developed as drugs. The purpose of this study is to identify Indonesian phytochemicals that can act as hepcidin antagonist. Methods: The research was a bioinformatics study with molecular docking method. Three-dimensional structure of human hepcidin as a target protein and Anticalinas a standar therapy were downloaded from the website of the Protein Data Bank (PDB). The active compounds were obtained from HerbalDB and the three-dimensional structure was from PubChem National Center for Biotechnology Information (NCBI). This study was performing using AutoDock Vina 1.1.2 to analyze the binding affinity. Molecular modification was performed with Autodock Tools 1.5.6. Visualization was done with Chimera 1.10 and PyMol 1.3. Results: The docking scores between hepcidin and Anticalin was -4.6 kcal/mol at Cys13,Cys14, Arg16, and Ser17. The compound Roxburghine B and Oxoxylopine could interact with the ligand binding domain and had the docking scores lower than Anticalin. Conclusions: Roxburghine B and Oxoxylopine are potential to become hepcidin antagonists insilico for inflammatory anemia. Keywords: anemia inflamasi, phytochemical, molecular docking, hepcidin, Anticalin

In Vitro Antihelmintic Effect of Mahagoni (Swietenia mahagoni Jacq) Seeds Etanol ExtractAgainst Ascaris suum Goeze Yusuf Arif Salam; Yulia Sari; Sigit Setyawan
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Introduction: Mahagoni seeds contain flavonoid, saponin, and tannin that that have been known having anthelmintic effect. This study aimed to determine the antihelmintic effect of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract against Ascaris suum Goeze in vitro. Methods:The study was a laboratory experimental research using post test only controlled group design. The subject were adult Ascaris suum Goeze worms. The sampling technique used was quota sampling. This research was using mahagoni (Swietenia mahagoni Jazq) seeds etanol extractwith concentration 30% w/v, 50% w/v, 70% w/v, 90% w/v, NaCl 0.9% (negative control), and albendazol 0.5% (positive control).Data were analyzed with Pearson correlation test to determine correlation between concentration of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract and amount of worms death. Probit analysis has been done to find LC50. Results: Pearson correlation test showed significant result p=0.00 and strong correlation r = 0.87. Probit analysis results showed LC50mahagoni (Swietenia mahagoni Jacq) seeds etanol extract was 40.66% w/v. It means that 40,66% concentration of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract can cause the death of half sample. Conclusions:Mahagoni(Swietenia mahagoni Jacq) seeds etanol extract had effects to the mortality of Ascaris suum Goeze in vitro with strong correlation between additional amount of extract concentration and amount of worms with LC50value= 40,66% w/v. Keywords :Antihelminthic, Swietenia mahagoni Jacq, alkaloid, Ascaris suum Goeze

Potency of Moringa Leaf Extract (Moringa oleifera, Lam.) in Lowering the fatty infiltration of Heart on Hypercholesterolemia Rat (Rattus norvegicus). Raden Ismail Hafidh Adinugroho; Endang Listyaningsih Suparyanti; Muthmainah Muthmainah
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Introduction: The high consumption of high cholesterol foods associated with increased mortality from cardiovascular disease. Because of the side effects like myopathy and the high price of hipercholestrolemia therapy, public interest for the use of natural medicine increased. Moringa leaves contain flavonoids, polyphenols, and ?-sitosterol so that it can reduce fatty infiltration at heart. Histological Study of effect moringa leaves on heart has never been performed before. This study aimed to determine whether the extract of Moringa leaf (Moringa oleifera Lam.) can lower the fatty infiltration of heart on hypercholesterolemia rat model (Rattus norvegicus). Methods: This study was an experimental laboratory with post test only control group design. A sample of male rats were consisting of Sprague Dawley rats, weight 150-200 g, aged 2-3 months. The sampling technique used is incidental sampling. Twenty eight of samples were randomly divided into four groups, each group consisted of 7 rats.Group KK is given aquades. Group K (-), K1, and K2 is given a high-fat diet for 42 days. Group K1 and K2 were each given Moringa leaf extract at a dose of 200 and 400 mg pe kg of body weight from day 42nd until day 70th. Termination was performed on 71th day, then their hearts were prepared for specimen stained with hematoxylin eosin. Heart ventricle fat area was observed with 1000x magnification on light microscope. The data were analyzed using Kruskal-wallis comparative test and then Post-hoc test Mann Whitney was performed (? = 0.05). Results: The observation area of fat at left and right ventricle in KK, K (-), K1, K2 were 378.8153.32; 1280.15133.94; 386.7457.87; and 326.129.89 ?m. Kruskall-Wallis test showed significant result (p = 0.000). Mann Whitney test results showed no statistically significant difference in area of ventricle fat (p <0.05) among all groups, except between groups of KK and K1 (p = 0.633). Conclusion: The extract of leaves of Moringa (Moringa oleifera Lam.) can reduce steatosis / fatty liver histopathology picture rat heart (Rattus norvegicus) model of hypercholesterolemia. Keywords:Moringa leaf extract, heart, fatty infiltration, rat.

Comparison of the effect of alkaline water and metformin towards blood glucose level in diabetic model rats Ghani Abdurahim; Ratih Dewi Yudhani; Siti Ma'rufah
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Introduction: Diabetes mellitus is a chronic disease, resulting from defects in insulin secretion, insulin action, or both. Indonesia ranked 7 out of 10 on countries with the most highest number of diabetic patient. Oxidative stress and high blood glucose level were occurring under diabetic condition. High prevalence of diabetes and expensive cost for treatment lead to development of alternative treatment for diabetes. Alkaline water with pH>7 acts as antioxidant and had been proven to lower blood glucose level via increase in glucose uptake. Metformin is the first line drugs for type 2 diabetes. The aim of this research was to compare the effect of alkaline water and metformin on blood glucose level of diabetic model rats. Methods: This research was experimental laboratory with the randomized pre and post test controlled-group design. This research was using 24 in total of 2 months old male Wistar albino rats weighting 180-200 g. Rats induced by alloxan 155mg/kg bw on the first day, then randomly divided into 4 groups with different treatments. Negative control group (K-) was given aquadest, positive control (K+) metformin 9mg/200g bw, Alkaline water 1 (Alk1) 23 ml, and alkaline water 2 (Alk2) 46 ml of alkaline water. These treatment was given once daily on the 5th until 12th day. Blood glucose level was measured three times: before induced by alloxan, after induced by alloxan, and post-treatment. Data were analyzed using repeated measures ANOVA (? = 0.05) followed by Multiple Comparisons LSD (? = 0.05).Result: The mean of post-treatment blood glucose level on K-, K+, Alk1, Alk2 respectively (in mg/dl) were 242.3350,06; 67.8312.90; 71.838,37; 70.839.72. The result of Repeated Measures Anova showed significant difference on mean blood glucose level (p=0.000). The result of LSD on mean of blood glucose level reduction showed significant difference (p=0.000) between K- and K+, K- and Alk1, K- and Alk2. The results of LSD showed no significant difference between K+ and Alk1 (p=0.536) as well as K+ and Alk2 (p=0.280).Conclusions: Alkaline water has comparable effect with metformin on blood glucose level of diabetic model rats.Keywords: alkaline water, metformin, blood glucose level, alloxan

Comparison of Hematology Analyzer Cyanmethemoglobin Method and Point-of-Care Testing (POCT) Electrode-Based Biosensor Method in Measurement of Hemoglobin LISANA SHIDQI; RATNA KUSUMAWATI; RATIH DEWI YUDHANI
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Introduction: Measurement of hemoglobin concentration can be done by hematology analyzer (HA) cyanmethemoglobin method (gold standard) and point-of-care testing (POCT) electrode-based biosensor method. POCT electrode-based biosensor method is one of the hemoglobin POCT and has not been studied before in Indonesia. This study examined the difference and correlation hemoglobin concentration by HA cyanmethemoglobin method and POCT electrode-based biosensor method. Methods: This was a cross sectional study in 4 senior high schools of Sukoharjo in Indonesia on Desember, 2016. Senior high schools were selected using a simple random sampling and senior high schools grade selected using a stratified random sampling. Among 173 grade 10 and 11 senior high school girls in this study the capillary and venous blood samples were collected. Capillary blood sample collected was immediately processed to measure the hemoglobin concentration using POCT electrode-based biosensor method and venous blood sample was collected to measure the hemoglobin concentration using HA cyanmethemoglobin method. Statistical analyses used were Mann-Whitney and Spearmans correlation coefficient (?=0.05). Results: Hemoglobin concentration determined by the POCT electrode-based biosensor method compared to HA cyanmethemoglobin method was significantly different (p=0.000 ) and there was positive moderate correlation (r=0.438; p=0.000). Conclusions: Hemoglobin concentration assessment by POCT electrode-based biosensor method has shown significantly different and positive correlation with HA cyanmethemoglobin method. Keywords: hemoglobin concentration, HA cyanmethemoglobin method, POCT electrode-based biosensor method

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Journal Mail Official
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Kota surakarta,

Jawa tengah

INDONESIA