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All Journal Jurnal Online Mahasiswa (JOM) Bidang Keguruan dan Ilmu Pendidikan Nexus Kedokteran Klinik Nexus Biomedika Nexus Kedokteran Translasional GIZI INDONESIA Jurnal JUMPED (Jurnal Manajemen Pendidikan) Jurnal Ilmu dan Teknologi Kesehatan Idea : Jurnal Studi Jepang EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Smart Society Empowerment Journal
Yulia Sari
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Hubungan Pemberian Obat Anti Tuberkulosis (OAT) dengan Kadar Asam Urat Ivan Setiawan; Ana Rima Setijadi; Yulia Sari
Nexus Kedokteran Klinik Vol 3, No 1 (2014): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Multidrug tuberculosis treatment which is a long term medication often causes various side effects. One of them is an increase of uric acid level. This study aims to determine the correlation between tuberculosis treatment administration and uric acid level. Methods: This study used cohort method with one group before and after intervention design. A total of 30 tuberculosis patients who were admitted to Center of Lung Health Society Surakarta between April and August 2013 were taken as samples by consecutive sampling method. Criteria of sampling are pulmonary tuberculosis patients who is planned to get rifampisin, isoniazid, pirazinamid, and ethambutol for more than 4 weeks, aged over 18 years old, and had given informed consent. In this study we excluded pulmonary tuberculosis patients who also had liver or renal disorders, had suffered from hyperuricemia or took drugs for decreasing uric acid level, or had hypertension. The measurements of uric acid level was collected before the treatment and more than one month after the treatment. Data were analyzed by paired t test (? = 0,05). Results: The average uric acid level of 30 patients before treatment was 4.68 1.12 mg/dL, while the average uric acid levels after treatment was 8.56 2.21 mg/dL, which was significantly higher than pretreatment (p=0.001). During treatment, hyperuricemia (uric acid level > 7 mg/dL) was reported in 73.33% (22/30) of patients and two patients had arthralgia. Conclusions: Tuberculosis treatment administration may affect uric acid level. Hiperuricemia was reported in 73.33% of patients who receive tuberculosis treatment. Keywords: tuberculosis treatment, uric acid level, tuberculosis
Pengaruh Pemberian Ekstrak Etanol Bit Merah (Beta vulgaris) terhadap Kerusakan Histologis Sel Ginjal Mencit (Mus musculus) yang Diinduksi Parasetamol Amanda Yessica Anggara; . Muthmainah; Yulia Sari
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Kidney failure can happen due to intoxication of paracetamol that causes oxidative stress. Red beet (Beta vulgaris) containing various components such as vitamin A, C and E, flavonoid, Cu, Zn, Mn, selenium, betanin, and phenolic has a function as antioxidants. The aims of this research were to know the nefroprotective effect of ethanol extract of red beet to prevent renal cell damage of mice. Methods: This was laboratory experimental research with posttest only controlled group design. Subjects of this research were thirty Swiss webster male mice (Mus musculus), 2-3 months old and 20 grams of body weight (BW), each.. The subjects were divided into 5 groups randomly. The groups of this research were negative control (KK(-)), positive control (KK(+)), experimental group 1 (KP1), experimental group 2 (KP2), and experimental group 3 (KP3). The KP1, KP2 and KP3 were administered 5.6 mg, 11.2 mg and 22.4 mg per 20g BW, successively. The nefroprotective effect of ethanol extract of red beet to prevent renal cell damage of mice was assessed by counting the number of damage cells among 50 renal cells on pars convulata. Results: One-Way ANOVA analysis p=0.000 (p<0.05) followed by Post Hoc Multiple Comparisons showed a significant difference between negative control and other groups. The increasing dose of the ethanol extract of red beet was followed by the improvement of the protective effect. The linear regression test p=0.000 (p<0.05) showed a correlation between the increase of dose of ethanol extract of red beet and the improvement of the nefroprotective effect to the histological damage of mices renal cells. Conclusions: Ethanol extract of red beet showed the nefroprotective effect to the histological damage of mices renal cells induced by paracetamol. The increase of dose of the ethanol extract of red beet improved the protective effect to mices renal cells. Keywords: ethanol extract of red beet, histological damage of mices renal cells, paracetamol
Pengaruh Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth.) terhadap Kerusakan Struktur Histologis Ginjal Mencit (Mus musculus L.) yang Diinduksi Parasetamol Lina Kristanti Wibowo; . Suyatmi; Yulia Sari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Kenikir leaves contain various kind of antioxidants, such as vitamin C, ?-carotene, minerals, and polyphenols which are known to protect kidneys from oxidative stress caused by free radical exposure. The purpose of this study is to evaluate the nephroprotective effect of Kenikir leaf extract toward histological damage of the mice's kidney cells induced by Parasetamol. Methods: This was a laboratory experimental study with the posttest only controlled group design. The samples were 35-Swiss webster male mice, aged 2-3 months, and weight about 20 gram. Samples were divided into five groups randomly. Normal group (KN) was given distilled water and Negative control (KK(-)) was given Parasetamol. Extract dosage of 2.8 mg, 5.6 mg, and 11.2 mg of kenikir leaves were given daily for 14 days to treatment group 1 (KP1), treatmen group 2 (KP2), and treatment group 3 (KP3), respectively. A toxic dosage of Parasetamol as much as 5.07 mg, was given to negative control group and treatment groups on day 12th, 13th, and 14th. On day 15th , mice were sacrificed by neck dislocation. The kidneys were taken for histological preparation and stained with HE. The histological damage was assessed based on quantifying the pyknosis, karyorrhexis, and karyolysis on 50 proximal tubuli of epithelial cells. The data were analyzed using One-way ANOVA test (? = 0.05) and Post Hoc Multiple Comparisons test (? = 0.05). Results: The most damaged cells were found in KK(-) and the least in KN. The significant difference was observed among groups (One-way ANOVA test p = 0.000). The significant difference was observed between groups (Post Hoc test p = 0.000). They were KN-KK(-), KN-KP1, KN-KP2, KN-KP3, KK(-)-KP1, KK(-)-KP2, KK(-)-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusions: Ethanol extract of Kenikir leaves can prevent the histological damage of the mice's kidney cell induced by Parasetamol. The increasing dose of the extract is followed by the increase of protective effect. Keywords: Kenikir leaves, histological damage, mice's kidney, Parasetamol
Analisis Molekuler Regio Core Promoter dan Precore/CoreIsolat Virus Hepatitis B 09IDSKAB-3 Ibnu Yudistiro; Afiono Agung Prasetyo; Yulia Sari
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: HBV replicates its DNA genome through reverse transcription from RNA intermediate. It is vulnerable to a high number of mutations during such reverse transcription which are frequently found in core promoter and precore/core regions. This study was aimed to identify genetic variation of HBV core promoter and precore/core regions of 09IDSKAB-3 isolate. Methods: DNA extraction was performed on 09IDSKAB-3 blood sample that was taken from Man Sex with Man Community. Core promoter and precore/core regions were determined by PCR using KL-28 and KL-6 primers and direct sequencing of the corresponding region. Molecular analysis was performed using MEGA 4.0. Results: Based on BLAST result, 09IDSKAB-3 HBV isolate had the highest similarity to isolate AP011085 from DKI Jakarta. Genetic variations A1726C in core promoter, and T1860C, C1877T, G1957C in precore/core region were found in 09IDSKAB-3 isolate. Conclusions: 09IDSKAB-3 HBV isolate was classified into genotype B and subgenotype B3 based on core promoter and precore/core region. The genetic variations found in this isolate may have influence to the replication efficiency and HBeAg/HBcAg production, therefore need further study. Keywords: hepatitis B virus, molecular analysis, core promoter region, precore/core region
In Vitro Antihelmintic Effect of Mahagoni (Swietenia mahagoni Jacq) Seeds Etanol ExtractAgainst Ascaris suum Goeze Yusuf Arif Salam; Yulia Sari; Sigit Setyawan
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

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Introduction: Mahagoni seeds contain flavonoid, saponin, and tannin that that have been known having anthelmintic effect. This study aimed to determine the antihelmintic effect of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract against Ascaris suum Goeze in vitro. Methods:The study was a laboratory experimental research using post test only controlled group design. The subject were adult Ascaris suum Goeze worms. The sampling technique used was quota sampling. This research was using mahagoni (Swietenia mahagoni Jazq) seeds etanol extractwith concentration 30% w/v, 50% w/v, 70% w/v, 90% w/v, NaCl 0.9% (negative control), and albendazol 0.5% (positive control).Data were analyzed with Pearson correlation test to determine correlation between concentration of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract and amount of worms death. Probit analysis has been done to find LC50. Results: Pearson correlation test showed significant result p=0.00 and strong correlation r = 0.87. Probit analysis results showed LC50mahagoni (Swietenia mahagoni Jacq) seeds etanol extract was 40.66% w/v. It means that 40,66% concentration of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract can cause the death of half sample. Conclusions:Mahagoni(Swietenia mahagoni Jacq) seeds etanol extract had effects to the mortality of Ascaris suum Goeze in vitro with strong correlation between additional amount of extract concentration and amount of worms with LC50value= 40,66% w/v. Keywords :Antihelminthic, Swietenia mahagoni Jacq, alkaloid, Ascaris suum Goeze
Efek Nefroprotektor Ekstrak Biji Mahoni (Swietenia mahagoni (L.) Jacq.) terhadap Kerusakan Histologis Sel Ginjal Mencit (Mus musculus) yang Diinduksi Parasetamol Muvida Muvida; . Muthmainah; Yulia Sari
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Seed extract of Swietenia mahagoni (L.) Jacq. contains saponins, flavonoids, alkaloids, terpenoids, steroids, and tannins that may exhibit significant protection of kidney cells from free radicals. In present study, Swietenia mahagoni was evaluated for its nephroprotector effect on paracetamol-induced kidney cells histological damage in mice (Mus musculus). Methods: This research used experimental laboratory studies with post test only controlled group design. Samples were 28 male Swiss webster mice (2-3 months old) weighing + 20 g and divided into 4 groups, 7 mice each group. Sampling technique in this research was incidental sampling. The negative control group (KK (-)) and the positive control group (KK (+)) of mice were administered aquadest for 14 days. The first treatment group (KP1) and the second group treatment (KP2) of mice were administered mahagony seed extract with the dose of 11,2 mg/20 g and 22,4 mg/20 g body weight of mice for 14 days, respectively. Paracetamol was administered to groups of KK (+), KP1, dan KP2 on the 12th, 13th, and 14th day. On day-15th, mice were sacrificed and their kidneys were taken and stained with hematoxilin eosin (HE) for histological study. The kidney cells histological features were assessed based on quantifying of pyknosis, karyorrhexis, and karyolysis. Data were analyzed with the One-Way ANOVA test (? = 0.05) and Post Hoc Multiple Comparisons LSD test (? = 0.05). Results: The mean of kidney cells histological damage in mice for KK (-), KK (+), KP1 and KP2 were 9.57 + 0.701; 28.93 + 1.698; 9.28 + 0.873 and 27.79 + 1.651, respectively. Result of statistic analysis showed that there were significant differences of kidney cells damage score between KK (-) KK (+), KK (-) KP2, KK (+) KP1, KP1 KP2, and non significant differences between KK (-) KP1 and KK (+) KP2. Conclusions: Swietenia mahagoni (L.) Jacq. seed extract showed nephroprotector effect against paracetamol-induced kidney cells histological damage in mice. Keywords: mahagony seed extract, nephroprotector, kidney cells histological damage
Analisis Molekuler Regio Pre-S1, Pre-S2, dan S Isolat Virus Hepatitis B 09IDSKAB-3 Angga Dwi Prasetyo; Afiono Agung Prasetyo; Yulia Sari
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: The Hepatitis B Virus (HBV) pre-S1, pre-S2, and S region are essentially known for virus infection and replication. Eight genotypes (A to H) and nine subtypes (adw2, adw4, ayw1, ayw2, ayw3, ayw4, adrq+, adrq-, and ayr) of HBV have been identified worldwide. The aim of this study was to analyze HBV genetic variation in pre-S1, pre-S2, and S region, and to determine genotype and subtype of 09IDSKAB-3 HBV isolate from Men Who Have Sex With Men community in Surakarta. Methods: 09IDSAKAB-3 HBV DNA extraction was used as a template for amplication of pre-S1, pre-S2, and S region. The sequence results were then aligned by Clustal W with all of reference sequences reported in GenBank/DDBJ/EMNL. Genetic variation in pre-S1, pre-S2, and S region were identified using MEGA 4.0. HBV genotype and subgenotype were identified by phylogenetic analysis. HBV subtype was deduced on the basis of the predicted amino acid sequences of HBsAg. Results: Based on BLAST search in GenBank, 09IDSKAB-3 isolate was classified into genotype B3. D27E variation was found in pre-S1 region, and there were not genetic variation in pre-S2 and S region. Phylogenetic tree showed that 09IDSKAB-3 isolate was classified into genotype B3. Based on the basis of predicted amino acid sequences of HBsAg, 09IDSKAB-3 isolate was classified into subtype adw2. Conclusions: Overall, only D27E variation was found in this study. 09IDSKAB-3 isolate was classified into genoytpe B3 and subtype adw2. The amino acid variations found in the present report need further study. Keywords: HBV, pre-S1 region, pre-S2 region, S region
The Effect of Strawberry (Fragaria x ananassa) Fruit Juice on Renal Cell Histological Damage of Mice Induced by Rhodamine B Dea Saufika Najmi; Endang Listyaningsih Suparyanti; Yulia Sari
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background:Strawberry (Fragaria x ananassa) contains many antioxidant phytochemicals such as vitamin C, anthocyanins and ellagic acid that may exhibit significant protection of kidney cells from free radicals. In present study, Strawberry (Fragaria x ananassa) fruit juice was evaluated for its nephroprotector effect on mice renal damage induced by Rhodamine B. Methods:This research was an experimental laboratory study with the posttest only controlled group design. Samples were 28 male mice, Swiss webster type, 2-3 months old and 20 g of each weight. Samples were divided into 4 groups of 7 mice each. This research used consecutive sampling. Strawberry juice was given for 16 days in a row, while Rhodamine B (0.2 ml/20 gr of mice body weight) was given on 10th to 16th day. Negative Control Group (KK-) was given distilled water only. Positive Control Group (KK+) was given Rhodamine B. First Treatmennt Group (KP1) and Second Treatment Group (KP2) were given 0.4 ml and 0.8 ml of Strawberry fruit juice per 20 gr of mice body weight and Rhodamine B. On the 17th day, mice were sacrificed by neck dislocation. After that, the kidney was dissected out and made for preparation with paraffin block method and stained with Hematoxilin Eosin (HE). The score of renal cells damage was determined by pyknosis, karyorrhexis, and karyolysis. The data were analyzed with One-Way ANOVA test (? = 0.05) and Least Significant Differences (LSD) test (? = 0.05). Results:The result of One-Way ANOVA test showed that there was a significant difference in score of renal cells (p < 0.05). LSD test showed a significant difference in score of renal cells (p < 0.05) across all pair groups. Conclusions:Strawberry (Fragaria x ananassa) fruit juice showed nephroprotector effect against renal cell histological damage in mice induced by Rhodamine B. Keywords:Strawberry fruit juice, Rhodamine B, renal cell histological damage.
Efek Proteksi Biji Mahoni (Swietenia mahagoni Jacq.) terhadap Kerusakan Hepar Mencit yang Diinduksi Parasetamol Dahniar Endahfuri; Endang Listyaningsih; Yulia Sari
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Seedof Swietenia mahagoni Jacq. contains alkaloids, saponins, flavonoids, triterpenoids, steroids, and tannins which were thought to protect liver from free radicals.In present study, Swietenia mahagony Jacq.seed extract was evaluated for its protective effect on paracetamol-induced liver damage in mice. Methods:To evaluate thehepatoprotectiveeffects of Swietenia mahagony Jacq. seed extract, 28 male Swiss webster micewere equallydividedinto fourgroups. Animals of group I (K) and group II (P1) were given aquadest for 14 days. Group III (P2) received 14 mg/25 g body weight of mice of Swietenia mahagony Jacq. seed extract and the last group (P3)received 28 mg/25 g body weight of mice of Swietenia mahagony Jacq. seed extract for 14 days.Paracetamol was given to groups of P1, P2, andP3.Mice were sacrificedon the 15th day and histological preparation was made to evaluate histological damage on liver. Liver histological features were assessed by counting the number of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. Data were analyzed using the OneWay ANOVA test (? = 0.05) and then were continued with Post Hoc Multiple Comparisons (LSD) test (? = 0.05). Results:The mean liver histological damage score was significantly higher on group of P1=60.71+7.521, whilegroup of K= 9.86+1.574 as the less.P2grouphad fewer numbers of liver histological damage (32.57+2.573) compared to the P3group(43.57+3.101). The results of OneWay ANOVA and LSD test showed that there was significant difference between the four groups (p = 0.000). Conclusions:Swietenia mahagony Jacq.seed extract showed protective effect against the hepatotoxicity induced by paracetamolin mice.Swietenia mahagony Jacq.seedextract with the dose of 14 mg/25 g bodyweight of mice showed a higher hepatoprotective effect than the dose of 28 mg/25 g body weight of mice. Keywords:Swietenia mahagony Jacq. seed, liver damage,paracetamol
The Effect of Mango (Mangifera indica L.) Juice on Paracetamol Induced Liver Cell Damage in Mice (Mus musculus) Samiaji Abbas Ras; Endang Listyaningsih; Yulia Sari
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Mango (Mangifera indica L.) contains beta-carotene and vitamin C which were thought to protect the liver from free radicals. In present study, Mango (Mangifera indica L.) juice with certain dose was evaluated for its protective effect on paracetamol induced liver damage in mice. Methods: This study used 28 male Swiss Webster mice were equally divided into four groups. Animals of Control Group (KK) were given aquadest and Treatment Group I (KP1) received paracetamol for 3 days. Treatment Group II and III (KP2 and KP3) not only received paracetamol, but also received multilevel doses of Mango (Mangifera indica L.) juice (0,2 mg and 0,4 mg) for 14 days. Mice were sacrificed on the 15th day and histological preparation was made to evaluate histological damage on liver. Liver histological features were assessed by counting the number of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. Data were analyzed using the One Way ANOVA test (? = 0.05) and the Post Hoc Multiple Comparisons (LSD) test (? = 0.05). Results: The mean liver histological damage score was significantly higher on group of KP1= 61,87.991, while group of KK= 26,214.828 as the least. KP3 group had fewer numbers of liver histological damage (40,097.635) compared to the KP2 group (37,815.653). The results of One Way ANOVA test showed significant difference between the five groups (p = 0.000) and the results of LSD test showed significant differences between each other group (p < 0.005). Conclusion: Mango (Mangifera indica L.) juice showed protective effect against the hepatotoxicity induced by paracetamol in mice. Mango (Mangifera indica L.) juice with the dose of 0,4 mg/20 g body weight of mice showed a higher hepatoprotective effect than the dose of 0,2 mg/20 g body weight of mice. Keywords: Mango juice, paracetamol, histologic liver damage.
Co-Authors . Muthmainah . Suyatmi Afiono Agung Prasetyo Alvian Oscar Irawan Amanda Yessica Anggara Ana Rima Setijadi Angga Dwi Prasetyo Annisa Avelia Arina Setyaningrum Asril &#039; Budi Afriyansyah Dahniar Endahfuri Dea Saufika Najmi Debora Marga Pangestika Dian Ariningrum Diana Hartaty Angraini Didik Gunawan Tamtomo Ekkim Al Kindi Emma Ayu Lirani Endang Listyaningsih Endang Listyaningsih S Endang Listyaningsih Suparyanti Fakhri Ras Helen Susanti Ibnu Yudistiro Ivan Setiawan Kamarrudin Oemar Leli Saptawati Lilik Wijayanti Lina Juairiah Lina Kristanti Wibowo Makhdalena Makhdalena Muvida Muvida Rina Fitriana Risky Pratiwi Samiaji Abbas Ras Sigit Setyawan Siti Marufah Sri Haryati Sugeng Purnomo Widia Susanti Yusuf Arif Salam