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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Education,
Education
Welcome to the Research Journal of Life Science, published by the Institute of Research and Community Service UB. For further information please contact our editorial office.
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Articles 8 Documents
 1 
Search results for , issue "Vol 8, No 1 (2021)" : 8 Documents clear
Design and Effect of Escape Vent in a Trap on the Catch of Blue Swimming Crab (Portunus Pelagicus): A Preliminary Study Muhammad Arif Rahman; Feni Iranawati; Abu Bakar Sambah
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.2

Abstract

Blue Swimming Crab (Portunus pelagicus, BSC) is one of the Indonesian important fisheries commodities often captured by traps. Under Decree No. 56/2016, the Ministry of Marine Affairs and Fisheries managed the BSC, with the standard size of BSC is >10cm of Carapace Width (CW) or >60 g of Weight (W), and non-berried or carried eggs for female. This study aims to determine BSC's crawling pattern toward the trap, design an escape vent on the trap, and assess catch of BSC using vented trap and non-vented trap. The video footage was used to examine crawling pattern of 30 BSCs on the laboratory. The study also includes field experiment by deploying twenty traps (10 vented traps and 10 non-vented traps) on the sea. The study obtained two crawling patterns of BSC, crawling forward and sideways with the most BSC crawled sideways. Design of escape vent was 4.6 cm length x 2.6 cm height. The BSCs captured in the vented traps have >10cm of CW, while 14% of BSCs in the non-vented traps have <10 cm of CW. The use of escape vent did not affect the catch (weight) of BSC in the trap.
Optimized Expression Condition of CIDRα-PfEMP1 Recombinant Protein Production in Escherichia coli BL21(DE3): A Step to Develop Malaria Vaccine Candidate Winnie Almira Setyoadji; Erma Sulistyaningsih; Irawan Fajar Kusuma
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.3

Abstract

Malaria is still an essential epidemiological disease worldwide, including in Indonesia. Several approaches are performed to control the disease, as well as vaccine development. The Cysteine-rich interdomain region α of Plasmodium falciparum erythrocyte membrane protein 1 (CIDRα-PfEMP1) is a pivotal domain in the malaria pathogenesis make it a malaria vaccine candidate. The development of the malaria vaccine is performed using recombinant technology. Recombinant protein production is an important step. The study aimed to determine the optimized condition for CIDRα-PfEMP1 recombinant protein expression in Escherichia coli BL21(DE3) expression system. Serial IPTG concentrations from 0.05, 0.1, 0.3, and 0.5 mM and two different incubation periods of 4 h and 8 h were optimized. The recombinant protein expression was visualized in SDS-PAGE, measured using the Bradford protein assay, and calculated using software Image J. SDS-PAGE visualization showed a 27 kDa band expressed CIDRα-PfEMP1 recombinant protein. The optimized condition for CIDRα-PfEMP1 recombinant protein expression was at 0.03 mM IPTG concentration and 8 h incubation period.
The Potency of UB Forest Chitinolytic Bacteria to Promote Plant Growth and Inhibit Damping off Disease on Soybean Achmad Roekhan; Ayu Ike Dayanti; Rahmania Oktaviani; Fibrianti Shinta Dewi; Nabilla Alya Anastasya; Luqman Qurata Aini
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.4

Abstract

Damping off disease in soybean plants is caused by the fungal Rhizoctonia solani. The damping off disease causes a yield loss of up to 85-100%. The purpose of this study was determining the ability chitinolytic bacteria consortium of UB Forest's in suppressing damping off disease in vitro and in vivo as well as its potential to stimulate the growth of soybean plants. The research stages included isolation of the pathogenic fungus R. solani and the pathogenicity test. Rejuvenation chitinolytic bacterial isolates of UB Forest, test chitinolytic bacteria antagonist of UB Forest against R. solani fungus, test of Plant Growth Promoting (PGP) activities, synergy test of selected chitinolytic bacterial isolates, in vitro test for the inhibition of chitinase crude extract against R. solani, and inhibition test of chitinolytic bacteria consortium against damping off disease. The selected chitinolytic bacteria were code bacteria UB12, UB19, and UB52 with plant growth promoting activities with inhibition percentage of the pathogen R. solani of 73.9%, 67.4%, and 71.7%. The best chitinolytic bacterial isolates were the genus Bacillus sp. and Pseudomonas sp. The inhibition test of chitinase crude extract showed an inhibition percentage of 25-55%.
Effect of Prenatal Yoga on Duration of the First Stage of Labor and Perineal Rupture in Primigravida Mothers Asri Daniyati; Shohipatul Mawaddah
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.5

Abstract

In primigravidas, the first stage of labor has a longer duration than multiparous. The first stage of labor in primiparous is around 13-14 hours, while in multiparous it is about 7 hours. Prolonged labor is one of the causes of maternal death. Yoga in pregnancy can maintain elasticity and strength of the ligaments of the pelvis, hips, and leg muscles, so that it can reduce pain during labor and provide space for the birth canal.  The comparative analytical research method used pre-experimental design with the one shot case study design. With a sample of 26 trimesters III primigravida pregnant women who were divided into 2 groups, namely n = 13 groups who did prenatal yoga, and n = 13 groups who did not do prenatal yoga. The analysis of this research was univariate and bivariate using the Mann Whitney test. Assessment is carried out during the delivery process. As a result, the intervention and control groups with active phase I labor duration obtained p = 0.000, and perineal rupture p = 0.000. There was a significant difference in the length of the first stage of labor and perineal rupture between the yoga group and the non-yoga group. Prenatal yoga affects the duration of the first stage of labor and perineal rupture.
The Biological Aspects Comparison of Nila Tilapia (Oreochromis Niloticus) on The Eutrophic and Oligotrophic Reservoir from Indonesia Diana Arfiati; Nadya Agustarina Saputri; Nimas Styaningrum; Asthervina Widyastami; Karina Farkha Dina; Shofiyatul Lailiyah; Rizky Kusma Pratiwi; Zakiyyah Nur Inayah
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.1

Abstract

Comparison of the length, weight and condition factor of Nila Tilapia (Oreochromis niloticus) was studied on oligotrophic waters (Wonorejo Reservoir) conducted from July to August 2018 and eutrophic waters (Selorejo Reservoir) on February to March 2019. All of the reservoir are in the East Java, Indonesia. The total sample from Wonorejo Reservoir about 79 fish from 3 times sampling (every two weeks), but the fish Sample from Selorejo Reservoir are 35 from 4 times sampling every week. The Nila Tilapia from Wonorejo Reservoir are 15.3 ± 2.5 cm (length) and 74.6 ± 38.6 grams (weight) but fish from Selorejo are 17.8 ± 2.2 cm average length and 114 ± 35 grams for the weight. The condition factor of Nila Tilapia in both reservoirs was relatively the same, with an average value was 1.01. The Nila Tilapia caught from Selorejo Reservoir (eutrophic) is relatively heavier and bigger than in the Wonorejo Reservoir (oligotrophic) even though the hook size is smaller. Fish from the Selorejo Reservoir (eutrophic) is easier to get food so that the average fish is larger.
Potential Analysis of Lemna sp. Extract as Immunostimulant to Increase Non-Specific Immune Response of Tilapia (Oreochromis niloticus) against Aeromonas hydrophila Eric Armando; Ayu Lestiyani; R. Adharyan Islamy
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.6

Abstract

Lemna sp. is known to have several bioactive compounds and polysaccharide macromolecules that can function as immunomodulators to affect non-specific immune responses to increase the body's resistance to pathogens. This study aims to determine the potential of catfish eye extract as an immunostimulant by observing non-specific tilapia immune parameters. The extraction method used was 96% ethanol maceration for 2 days with a ratio of 1: 4. The experimental design used a Completely Randomized Design with 5 treatments (doses 0.2, 0.4, 0.6 mg/kg, control + and control -) and 3 replications. The non-specific parameters of immunity observed included total plasma protein (Bradford method), superoxide dismutase and lysozyme activity. The data obtained will be analyzed using ANOVA, if there is a significant difference, it will be further tested with Duncan Multiple. Range Test (DMRT). The results showed that the highest total plasma protein was found in treatment C (giving an extract of 0.3 mg/kg body weight) with an average total plasma protein after 12 days of maintenance of 4.99 g / dL. The extract dose of 0.3 mg/body weight showed a rapid decrease in SOD and increase Lysozyme activity.
Nematicidal Activity of Turmeric Extract against Nematodes Meloidogyne spp. Maria Ulfa; Toto Himawan; Hagus Tarno
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.7

Abstract

In an effort to reduce the impact of synthetic nematicides, a new formula was developed using plant-based nematicides that are more environmentally friendly, one of the basic ingredients that can be used is turmeric. The purpose of this study was to determine the effectiveness of turmeric extract (Curcuma domestica) with various different solvents namely aquades, methanol, and n-hexane at concentration of 5%, 10%, 15% and 20% respectively in inhibiting the activity of Meloidogyne spp. The experiment used was a Completely Randomized Design (CRD). Each treatment was repeated 4 times with 4 controls so that there were 52 experimental units. The result of different research data was analyzed using Analysis of Variance (ANOVA) which was then further tested with Honest Significant Difference (HSD) at the 5% level if real, and t-test for in vivo test. Based on the tested extract, it was found that turmeric extract with various solvent (aquades, methanol, and n-hexane) was effective in inhibiting the activity of Meloidogyne spp. nematodes outside the roots (hatching eggs and penetration), turmeric extract didn’t affect the activity of Meloidogyne spp. in the roots (development and reproduction), vegetable nematicides which the most active in suppressing the population and inhibiting the activity of nematodes was turmeric extract using methanol as a solvent at a concentration of 20%.
Effectiveness of Organic Pesticides made from Cigarette Stub to the Attack Pest Intensity of Terminalia cattapa Yusuf Arya Yudanto; Syaviela Viagul Sams Primartu; Dinana Anissatul Fuadiyah; Fahmi Arifan
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.8

Abstract

The high consumption of cigarettes, especially in Indonesia, will produce harmful waste for the environment called cigarette stubs.  The waste of cigarette stubs reacts with any kinds of pathogenic fungus such as Fusarium oxysporum, Colletotrichum gloeosporiodes, Rigidoporus lignosus, and Sclerotium rolfsii. The usage of cigarette stubs as an alternative raw material for making organic pesticides can make cigarette stubs considerable as a recycled product that reduces the environment's waste. The results obtained by plants treated with biopesticides effectively resist pest attacks and support plants to naturally develop antibodies. The score of Intensity Attack (IS) from Terminalia cattapa plant applied by the biopesticides for 10 days observation results in the amount of 3.953%, with a total of 34 normal leaves and a total of 12 broken leaves. The other result of the Terminalia cattapa applied with chemical pesticides gives 12.31% of intensity attack and 12.18% of intensity attack without any treatment. This research concluded that tobacco could inhibit the attack pest of the Terminalia cattapa plant.

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