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Research Journal of Life Science
Published by Universitas Brawijaya
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Welcome to the Research Journal of Life Science, published by the Institute of Research and Community Service UB. For further information please contact our editorial office.
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Articles 6 Documents
Search results for , issue "Vol 8, No 2 (2021)" : 6 Documents clear
Coral Reefs Health Status in the East Java: a Case Study in Banyuwangi, Situbondo, Probolinggo Pratama Diffi Samuel; Citra Satrya Utama Dewi; Nur Fadholi Ulul Azmi; M.Choirul Anam; Imas DP; Dewi Nur Setyorini; Dyah Fitri Hariani; Riska Fatmawati
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.1

Abstract

Coral reef ecosystems are among the most productive natural biodiversity resources  in tropical coastal areas, within seagrass beds and mangrove forests. Besides, coral reefs have very high ecological impacts and economic value that are managed well and sustainably. Also, healthy ecosystems will undoubtedly provide more incredible benefits and values, so studies need to be conducted to determine the health of coral reefs. While P2O LIPI is an institution that is the guardian of coral reef health data in Indonesia, there is limited information related to the coastal areas of East Java that has not been widely reported. This study aimed to find out the percentage of living hard corals and the health status of the ecosystem at three reef sites: Banyuwangi, Situbondo, and Probolinggo. Field surveys were carried out in June-July 2019, using the Line Intercept Transect (LIT) method. The materials used in this study are Self-Contain Underwater Breath Apparatus-SCUBA diving equipment, roll meters, and an underwater camera. The results showed that the hard coral cover living in Banyuwangi accounted for 28.91%, Situbondo was 31.20%, and Probolinggo (34.77%). Therefore, this study interpreted that the coral reef ecosystem was a moderate category (25% - 49.9%).
Feeling Guilt and Happiness Police of The Regional Traffic Management Center (RTMC) of The East Java Regional Police to Their Families (Phenomenology Study) Yunus Adi Wijaya; Tita Hariyanti; Tina Handayani Nasution
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.2

Abstract

Guilts is the first and foremost in the form of emotional outbursts. Guilts is not a very good motivator. It is more accurate to regard guilt as an internal condition and a negative feeling. Happiness is a feeling of satisfaction, that life is as it should be and all needs have fulfilled. This study aims to find meaning in the experience of feeling guilt and happiness police of the Regional Traffic Management Center (RTMC) to their families. Qualitative interpretive phenomenology approach was conducted an in-depth interview with semistructured questions on five police officers at RTMC by purposive sampling conducted in December 2018. Based on Interpretative Phenomenological Analysis (IPA), this study found two themes namely: feeling guilty to the family and feeling happy with family. The findings obtained were that members of the police at RTMC could be free from stress while working and were able to return and get support from the family. Being able to live happily, both happy at work and happy with the family that will reflect in the calmness in their life.
The Effect of Water Treatment Models to Reduce Lead (Pb) Level on Freshwater Snail Filopaludina javanica Diana Arfiati; Nur Syahid; Zaki Anwari; Aminin Aminin; Kusriani Kusriani; Endang Yuli Herawati; Asthervina Widyastami Puspitasari
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.3

Abstract

Lead is a kind of non-essential heavy metals included in the metal causing environmental pollution with persistent properties that might harm the consumers. This study aimed to determine the best method for reducing the lead level on the freshwater snail Filopaludina javanica using three various water treatment models. Soaking water treatment (6h, 12h, 18h, 24h), flowing water treatment (6h, 12h, 18h, 24h), and refreshing water treatment (6h, 12h, 24h). The lead level assay in both samples used Atomic absorption spectroscopy (AAS), and the physical and chemical parameters were measured, such as temperature, pH, and Dissolved Oxygen (DO). The lowest value of lead content on freshwater’s soft body snail and water sample respectively were soaking water treatment at 6h (0.64 ± 0.02 mg L-1) and 24h (0.0045 ± 0.0015 mg L-1); flowing water treatment at 24h (0.04 ± 0.007 mg L-1) and 18h (0.0036 ± 0.0009 mg L-1) and; refreshing water treatment at 24h (0.150 ± 0.011) and 12h (0.007 ± 0.001), with control 0.072 ± 0.00 mg L-1 and 0.067 ± 0.00 mg L-1. Therefore, the most effective model to reduce the lead content was flowing water treatment within 24h in the freshwater soft body snail and 18h in the water sample.
Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The Growth of Aeromonas hydrophila Annisa Farhana Dewi
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.5

Abstract

Aeromonas hydrophila isgram-negative bacteria that can harm humans and major animals with poikilotherm properties such as fish and shrimp. These bacteria can cause hemorrhages in fish until death, causing fish farming production to fail. The use of medicinal plants has been trusted by people all over the world to overcome various disease problems, one of which is a disease caused by bacteria. Plantago major L. is known that able to inhibit the growth of both gram-positive and gram-negative bacteria. This study aims to find out the compounds contained in Plantago major L., using phytochemicals screening, FTIR, UV-VIS and antibacterial activity against A. hydrophila.This study extracts Plantago major L. known to contain polar compounds such as phenols, flavonoids, saponins, and tannins. The result of FTIR and UV-VIS strengthen the presence of phenols and flavonoids. Furthermore, extract Plantago major L. is known to be able to significantly inhibit the growth of A. hydrophila (P<0.05).
Effect of Histatin-1 Proteins to Fibroblasts Growth Factor-2 (FGF2) Concentrate and Fibroblasts Cell Migration on Human Gingival Fibroblast (HGF) Culture Ni Ketut Ayu Feriyanti Dewi; Nur Permatasari; Hidayat Sujuti
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.4

Abstract

The purpose of this study is to determine the effect of histatin-1 protein increasing the concentration of fibroblast growth factor (FGF2) and fibroblast cell migration in human gingival fibroblast (HGF) culture. The HGF cell culture used was obtained from odontectomy patients and was the result of the 4th passage. Cell culture was divided into 4 groups: HGF control group (without treatment) and treatment groups with 5µM, 10µM, and 20µM concentration of histatin-1 respectively. The amount of fibroblast growth factor 2 (FGF2) was quantified by using ELISA method. In vitro fibroblast cells migration was measured using scratch/wound healing assay method. FGF2 concentration and HGF cell migration were measured at hour 0, 3, 11, 24, and 48. The results of the One Way Anova statistical test difference between FGF 2 (ΔFGF2) at hour 24 showed a value of P = 0.042 (P≤0.05) and HGF cell migration at hour 11 showed a P value = 0.003 (P≤0.05). The conclusion of this study is that Histatin-1 Protein is able to increase the concentration of Fibroblast Growth Factor 2 (FGF2) and the migration of Human Gingival Fibroblast cells.
The Effect of Weather Elements on the Intensity of Leaf Blight Disease Caused by Phytophthora colocasiae in Taro Faishal Akbar; Ika Rochdjatun Sastrahidayat; Syamsuddin Djauhari
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.6

Abstract

Leaf blight Phytophthora colocasiae is one of the main diseases in taro plants which can reduce production yield significantly. Weather is one of the main factors supporting the epidemic of leaf blight. Weather changes will affect the development of pathogens and affect the development of disease in the field. This research aimed to find out the effect of weather elements (air temperature, humidity, sunlight intensity, rainfall, number of rainy days) on the intensity of the taro leaf blight attack. The results showed that weather elements had a significant correlation with the intensity of taro leaf blight disease. Air temperature and sunlight intensity showed negative correlations while air humidity, rainfall and the number of rainy days were positively correlated with the disease intensity. The results of this path analysis showed that air humidity had the most significant effect of the weather elements on the intensity of taro leaf blight both directly and indirectly.

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