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Jurnal Riset Kimia
Published by Universitas Andalas
ISSN : 1978628X     EISSN : 24768960     DOI : -
Core Subject : Science,
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Articles 6 Documents
Search results for , issue "Vol. 10 No. 1 (2019): March" : 6 Documents clear
PENJERNIHAN AIR SUMUR MENUJU AIR LAYAK MINUM DENGAN METODA LAPISAN MULTI MEDIA ( LMM ) Neneng Swesty; Rahmiana Zein; Z. Zilfa
Jurnal Riset Kimia Vol. 10 No. 1 (2019): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.297

Abstract

The Multi Soil layering (MSL) system is a liquid waste treatment method with the use of soil as the main medium and the organic material formed in brick and arranged in layers with a homogeneous zeolite layer. The MSL system is made of acrylic (50 x 15 x 50) cm containing a brick (4 x 9 x 15) cm and (4 x 5 x 15) cm of volcanic soil mixture as the main medium mixed with charcoal, rice husk, and iron powder (70 : 10 : 10 : 5)%, permiable layer is used zeolite (2-3) mm. The sample came from the well water of one of villager at Jati, Padang. Efficiency of SML system for aeration process on turbidity parameter with flow rate variation of 2, 4, 6, 8 mL/min reach 99,8 %, for non aeration process efficiency of turbidity decrease 88,14 %. While for the flow rate of 10, 20, 40, 80 mL/min, the efficiency of decrease for aeration at parameters Mn 66,44 %, nitrite 59,74 %, nitrate 58,34 %, turbidity 54,63 %, while in non aeration efficiency decrease turbidity reach 44,04 %, Mn 47,26 %, nitrite 49,74 %, nitrate 45,57 %. Of all efficiency values decreasing all parameters already complied drinking water quality standard according to Permenkes RI No. 492/Menkes/Per/IV/2010.
STUDI OPTIMASI EKSTRAKSI KANDUNGAN SENYAWA FENOLIK TOTAL DAN UJI AKTIVITAS ANTIOKSIDAN DARI DAUN MANGGIS (Garcinia mangostana Linn.) Emil Salim; Yogi Afritunando; Nindi Antika Febriana; Mai Efdi
Jurnal Riset Kimia Vol. 10 No. 1 (2019): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.308

Abstract

This study aims to determine antioxidant activity and total phenolic content of mangosteen leaves in several different solvents. The antioxidant activity test of the extract was determined by the DPPH method and the total phenolic content was determined by the Folin-Ciocalteu method. The phytochemical test of mangosteen leaves showed the presence of phenolic compounds, saponins, triterpenoids and alkaloids. The extraction results increase with increasing water content in methanol, ethanol and acetone. The highest amount of extract was obtained using 50% acetone solvent (34.98 ± 0.17%). Extraction using 100% ethanol showed the highest total phenolic content (614.25 ± 5.58 mg GAE / g extract) and the lowest IC50 value (5.81 mg / L). The results of this study indicate that the use of 50% acetone solvent can optimize the amount of extract and 100% ethanol can optimize the content of total phenolic compounds and antioxidant activity of mangosteen leaves.
ANALISIS UJI INFUSA BUAH PETAI CINA, DAUN KEJI BELING DAN DAUN TEMPUYUNG SEBAGAI INHIBITOR ENZIM α-AMILASE DAN α-GLUKOSIDASE Silvera Devi Sy; Musyima Rahmah Nst; Riryn Novianty
Jurnal Riset Kimia Vol. 10 No. 1 (2019): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.314

Abstract

Enzim α-amilase dan α-glukosidase dalam proses pencernaan akan  menghidrolisis amilum menjadi glukosa dan apabila glukosa darah melebihi batas normal (>140 mg/dL), maka seseorang didiagnosa menderita diabetes melitus. Pengobatan diabetes melitus khususnya tipe 2 biasanya diatasi menggunakan obat akarbose yang akan menginhibisi aktivitas α-amilase dan α-glukosidase. Pada penelitian ini akan dianalisis kemampuan inhibisi infusa dari sampel segar dan kering buah petai cina (Leucaena leucocephala L de Wit), daun keji beling (Strobilanthes crispus BI) dan daun tempuyung (Sonchus arvensis L.) terhadap ke 2 enzim ini. % inhibisi infusa terhadap aktivitas enzim α-amilase ditentukan menggunakan metode asam 3,5-dinitrosalisilat (DNS) sedangkan untuk α-glukosidase menggunakan substrat p-nitrofenil-α-D-glukopiranosida (p-NPG). Absorbansi hasil reaksi diukur menggunakan microplate reader pada panjang gelombang 530 nm untuk α-amilase dan 410 nm untuk α-glukosidase. Hasil penelitian menunjukkan bahwa % inhibisi infusa sampel kering lebih baik dibandingkan sampel segar  dalam menghambat aktivitas enzim α-amilase dengan persentase sebagai berikut: infusa buah petai cina kering 92,54+1,11%, infusa daun keji beling kering 99,79+6,92% dan infusa daun tempuyung kering 87,63+3,95%, nilai ini tidak berbeda nyata dengan akarbose 93,89+ 0,02%. Sedangkan % inhibisi terhadap aktivitas enzim α-glukosidase dari semua sampel memiliki perbedaan yang nyata dengan akarbose (P<0,05) dengan nilai inhibisi 97,99+ 0,19%. Hasil ini menunjukkan bahwa ketiga tanaman tersebut berpotensi sebagai antidiabetes terutama dalam menginhibisi aktivitas enzim α-amilase.
SINTESIS DAN KARAKTERISASI NUKLEOTIDA BERTANDA [α-32P]ATP Wira Yusril Rahman; Endang Sarmini; H. Herlina; A. Abidin; T. Triyanto; H. Hambali; Santi Nurbaiti
Jurnal Riset Kimia Vol. 10 No. 1 (2019): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.319

Abstract

The utilization of nuclear technology in health sector with molecular techniques is increasingly developed today, especially in Indonesia.  One of which is nucleotide compound marked with [α-32P]ATP, this compound has been used as tracer for deoxyribonucleic acid (DNA)/ ribonucleic acid (RNA) in the study of various physiological and pathological processes. [α-32P]ATP is synthesized through several stages of continuous reaction in one reaction vessel. It begins with synthesis of [γ-32P]ATP through an enzymatic reaction, using H332PO4 and ADP, and enzymes  of lactate dehydrogenase, 3-phosphoglycerate phosphokinase and glyceraldehide 3-phospho  dehydrogenase; followed by phosphorilation of 3’AMP with T4 polinucleotide kinase enzyme to produce 3’-[5’-32P]ATP. The result is hydrolyzed with nuclease P1 enzyme to produce [5’-32P]AMP. The unreacted [γ-32P] is degraded by the addition of hexokinase enzyme and glucose. At the final stage of the reaction, the [5’-32P]AMP is  phosphorilated using phosphoenol-piruvat, piruvat kinase, and myokinase to produce [α-32P]ATP. The test results show that the every stage of reaction is characterized using TLC method, PEI cellulose paper as stationary phase and KH3PO4 0,5 M pH 3,5 as mobile phase. At the end of reaction, the yield of [α-32P]ATP reaches 71,7%, at Rf = 0,2.
INHIBISI KOROSI BAJA RINGAN MENGGUNAKAN BAHAN ALAMI DALAM MEDIUM ASAM KLORIDA: Review Yeni Stiadi; Syukri Arief; Hermansyah Aziz; Mai Efdi; E. Emriadi
Jurnal Riset Kimia Vol. 10 No. 1 (2019): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.321

Abstract

The corrosion of steel and its alloys is an important problem in industry, especially in acidic environments. Mild steel is one of the important alloys of iron which has many industrial applications because of its excellent mechanical properties, but mild steel is susceptible to corrosion. Corrosion problems need to be a concern and must be handled properly. Hydrochloric acid is widely used for pickling, cleansing, decomposition and metal etching, on the other hand also contributes to corrosion of metal surfaces. Plant extracts investigated the properties, mechanisms of adsorption and efficiency of inhibition as environmentally friendly corrosion inhibitors for various steel materials in different acidic media. The efficiency of corrosion inhibition of steel from plant extracts analyzed ranged from 72-98% and generally as a mixed-type inhibitor. Most of the inhibitors are adsorbed on the steel surface through a physisorption mechanism.
ANALISIS SENYAWA BERBAHAYA PARFUM ISI ULANG YANG DIJUAL DI KOTA PADANG MENGGUNAKAN METODE KROMATROGRAFI GAS-SPEKTROMETRI MASSA Ridho Asra; R. Rusdi; Putut Arifin; N. Nessa
Jurnal Riset Kimia Vol. 10 No. 1 (2019): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.324

Abstract

Perfume is widely used by most of people in this world. The increasing demand of perfume has caused many producers cheating by adding dangerous compounds and also unregistered by the National Agency of Drug and Food Control Indonesia. The aim of this study is to analyze the dangerous compounds in unregistered perfumes sold in Pasar Raya Padang City by using gas chromatography-mass spectrometry (GC-MS) method. In this Study, samples (A, B, C, D and E) were collected and the value of specific weight and refractive index of the samples were analyzed. The results showed that five samples contained relatively similar chemical compounds. Twelve compounds were detected and seven of them were harmful to health in refill perfumes which were dipropylene glycol, linalool, lily aldehyde, benzenepentanol, dimethylbenzyl carbinyl acetate, dihydro methyl jasmonate, alpha hexyl cinnamic aldehyde, based on Material Safety Data Sheet (MSDS). Regular monitoring of chemicals used in the manufacture of perfumes which may cause health risks to users should be controlled by National Agency of Drug and Food Control Indonesia.

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