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Suriana
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alan H.E.A. Mokodompit, Kampus Baru, Jurusan Biologi Fakultas MIPA Universitas Halu Oleo Kendari
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Sulawesi tenggara
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BioWallacea Journal of Biological Research
Published by Universitas Halu Oleo
ISSN : 23556404     EISSN : 26856360     DOI : https://doi.org/10.1234/1234
Core Subject : Health, Agriculture,
Agriculture, Biological Sciences & Forestry Veterinary
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) telah memiliki ISSN 2355-6404 (print) dan ISSN 2685-6360 (online) yang merupakan salah satu jurnal nasional dan diterbitkan oleh Jurusan Biologi FMIPA Universitas Halu Oleo. Jurnal ini fokus pada ilmu biologi dan serumpun. Jurnal BioWallacea menerima naskah-naskah terbaik dari penulis yang bersifat asli hasil penelitian maupun telaah (review). Naskah ditulis dengan baik untuk setiap topik berkaitan dengan biologi yang berkembang saat ini serta bidang-bidang lain, termasuk: Penelitian Ekologi Fisiologi Ekofisiologi Taksonomi Botani Zoologi Mikrobiologi Biologi Laut Ilmu terapan berkaitan dengan ilmu Biologi seperti Bioteknologi, Biokimia, dan Biologi Sel serta Molekuler. Penelitian yang berkaitan isu lingkungan, termasuk Analisis Mengenai Dampak Lingkungan (AMDAL).
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 157 Documents
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SCREENING BAKTERI AMILOLITIK DAN SELULOLITIK DARI LIMBAH SAGU (Screening of Amylolytic and Cellulolytic Bacteria From Sago waste) Yanti, Nur Arfa; Munir, Asmawati
Jurnal BioWallacea Vol 1, No 1 (2014)
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Abstract

Screening of indigenous bacteria from sago waste based on amylolytic and cellulolytic activity was done to obtain bacterial isolate having double activity, i.e. could to hydrolize of starch (amylolytic) and cellulose (cellulolytic). Screening amylolytic and cellulolytic bacteria was done based on amylolytic and cellulolytic activity on agar media. Determination of amylolytic activity on starch agar media was based on the presence of clear zone around the bacterial colony upon flooding with lugol’s iodine solution. Cellulolytic activity was determine based on the presence of clear zone around the bacterial colony on Carboxy methyl cellulose (CMC) agar upon flooding with congo red solution. Presence of a clear zone around the colony indicated starch and cellulose hydrolysis. The diameters of clear zone produced on CMC and starch agar were measured and used as an indication of the amylolytic and cellulolytic activities of the bacteria. The results of the screening based on amylolytic and cellulolytic activity showed that a number of 21 bacterial isolates that having both activities. LCA2 was the bacterial isolate with the highest amylolytic and cellulolytic activity as revealed by the size of clearing zone on both types of agar plates. The diameters of clear zone on starch and CMC agar were 4,98   and 3,65 cm2, respectively. Therefore, LCA2 isolate was bacterial isolate that potent for biconvertion sago hampas into value-added products. Keywords : Bacteria, Amylolytic, Cellulolytic, Sago waste.
KEANEKARAGAMAN KUMBANG CERAMBYCIDAE (COLEOPTERA) DI KAWASAN GUNUNG MEKONGGA DESA TINUKARI KECAMATAN WAWO KABUPATEN KOLAKA UTARA PROVINSI SULAWESI TENGGARA(Diversity Of Cerambycidae Beetle (Coleoptera) In Mekongga Mount Area, In Tinukari Village Wawo Sub D ., Amirullah; Ariani, Citra; ., Suriana
Jurnal BioWallacea Vol 1, No 1 (2014)
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Abstract

The aim of this research is to determine the distribution, diversity, evenness and similarity of Cerambycidae bettle based on the total of species and individual at different altitude in mount of Mekongga area in Tinukari village Wawo sub district, north Kolaka regency in South East Sulawesi province. This research was used a survey method, catching Cerambycidae bettle by using sweep net, light trap, malaise trap, artocarpus trap and yellow pan trap by TCBG-LIPI mekongga team. Mounting and identification in laboratory entomology, sector zoology, biology research center Tndonesian of science, Cibinong Bogor, west java. The result show that there are 3 subfamilies of Cerambycidae bettle; Laminae (54 species), Cerambycidae (21 species) and Prioninae (1 species). The highest diversity index, was found at 0-500 asl (H=3,389) and the evennes high category was found at 1000-1500 asl (H=1). The highest similarity index of species was found at 0-500 and 500-1000 asl (47,225%).   Key words : Cerambycidae Bettle, Diversity, Mekongga Mount.
KARAKTERISASI FRAGMEN GEN 18S rRNA POKEA (Batissa violacea celebensis Martens, 1897) DI SUNGAI POHARA KECAMATAN SAMPARA KABUPATEN KONAWE (Characterization of 18S rRNA Gene Fragmen from Pokea (Batissa violacea celebensis Martens, 1897) in the Pohara Rive ., Muzuni; Adi, Dwi Arinto; Syarif, Satriani
Jurnal BioWallacea Vol 1, No 1 (2014)
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Abstract

This study aims to characterize sequences of 18S rRNA gene fragment from Pokea (Batissa violacea celebensis Martens, 1897) and its role in differentiated Pokea with other Bivalvia. The method used is a series of PCR (Polymerase Chain Reaction) reaction used to determine the sequence of 18S rRNA gene fragments Pokea. Analysis of the data using program NCBI (National Center for Biotechnology Information), Clustal X, Phydit (Phylogenetik editor), and TreeViewX for characterization 18S rRNA gene sequence to construct a phylogenetik tree of Pokea. The results showed that character of 18S rRNA gene fragments Pokea, namely: size 827 bp, including the family Corbicullidae because it has the closest kinship with Corbicula fluminea, as well as having restriction enzyme sites XhoI, PstI, BamHI, and DraI.   Keywords: Morphology, characterization, 18S rRNA gene, Pokea (Batissa Violacea celebensis Martens, 1897).
PENGETAHUAN DAN PEMANFAATAN TUMBUHAN OBAT TRADISIONAL MASYARAKAT SUKU MORONENE DI DESA RAU-RAU SULAWESI TENGGARA (Study and Utilization Of Traditional Medicine Plants By Morenene Ethnic In Rau-Rau Village, Southeast Sulawesi) ., Indrawati; Sabilu, Yusuf; Ompo, Alda
Jurnal BioWallacea Vol 1, No 1 (2014)
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Abstract

Research on the Study and utilization of Traditional medicine plants by Moronene ethnic in the Rau-Rau village Southeast Sulawesi were aims to: 1) inventory these kinds of medicinal plants are utilized including the identification of scientific and local names, 2) study the knowledge society in the utilization of plants as medicine, metods processing and the efficacy, 3) study the  knowledge kinds of disease and how to use of medicinal plants. With methods survey exploration conducted interviews to expert treatment (sandro) and the community. There are 51  spesies plant  in 27 family growing in the backyard, in the gardens and in the forests around the settlement. The organ  medicinal plant species (leaves, stems, bark, rhizome, tuber, fruit and SAP) are uses for tradisional medicines to cure about 36 kids of diseases. Method of use will discussed in this paper.   Keywords: Medicine Plants, Rau-rau Village Sociaty Moronene
Inventarisasi Capung (Odonata) di Sekitar Sungai dan Rawa Moramo, Desa Sumber Sari Kecamatan Moramo Kabupaten Konawe Selatan Sulawesi Tenggara (Dragonfly (Odonata) Stocktaking Around River and Moramo Swamp, Sumber Sari Village, Moramo District, South Ko ., Suriana; Adi, Dwi Arinto; Hardiyanti, Wa Ode Dian
Jurnal BioWallacea Vol 1, No 1 (2014)
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Abstract

The aim of this research was to know the dragonfly (Odonata) spesies around River and Moramo Swamp, Sumber Sari Village, Moramo District, South Konawe Regency, South-East Sulawesi. Dragonfly captured on three site namely river, swamp I and swamp II/ Moramo swamp. This research used descriptive method. There are 28 species of dragonfly which are include of 8 family namely Lindeniidae, Libellulidae, Megapodagrionidae, Lestidae, Coenagrionidae, Calopterygidae, Chlorocyphidae and Plactynemididae. Suborder Epiprocta found 13,33% family and 50% suborder Zygoptera of all families. There are 12 species in river, 15 species in swamp I and 13 species in swamp II/Moramo swamp. The Calopterygidae, Megapodagrionidae and Platycnemididae only found in river, whereas Lestidae (Lestes concinus Hagen) found in swamp only. The dragonfly spesies found in river were diffirent from swamp I and swamp II/Moramo swamp.   Key word:  Dragonfly, Odonata, Moramo Swamp, River,Stocktaking
IDENTIFIKASI MIKORIZA ANGGREK Spathoglottis plicata Blume. DAN Phalaenopsis amabilis L. IDENTIFICATION OF ORCHID MYCORRHIZA OF Spathoglottis plicata Blume. AND Phalaenopsis amabilis L. Ningsih, Rita; ., Dinarni; Febrianti, Dwi
Jurnal BioWallacea Vol 1, No 1 (2014)
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Abstract

Isolation and identification of mycorrhiza fungal that associated with Spathoglottis plicata Blumeand Phalaenopsis amabilis L. orchid root have be done. The descriptive research consist of threesteps that were isolation, purification and identification. Slice of roots were inoculated on PotatoDextro Agar (PDA) media for 2 – 5 days. The fungal colony were purified then identified basedon colony and morphology characters. The observed parameter were fungal colony, hypha andspore type. The result showed that there were 3 isolate found from root of terrestrial orchidSpathoglottis plicata Blume i.e SA1.1 was Chaetoium belonging to Ascomycetes class; SA2.2was Beltrania belonging to Ascomycetes class too and SA2.3 was Rhizoctonia belonging toDeuteromycetes class. Meanwhile the identification result of mycorrhiza fungal from root ofepiphytic orchid Phalaenopsis amabilis L. showed that 2 isolate i.e PA1.2 and PA1.3 both of themRhizoctonia belonging to Deuteromycetes class.Keywords : orchid mycorrhiza, Spathoglottis plicata Blume, Phalaenopsis amabilis L., orchid root
Keanekaragamaan dan Pemanfaatan Tumbuhan Obat Tradisional Pada Masyarakat di Kelurahan Lipu Kecamatan Betoambari Kota Baubau Provinsi Sulawesi Tenggara Indrawati Indrawati; Yusuf Sabilu; Puji Fitria Zainal
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 2, No 1 (2015)
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.201 KB) | DOI: 10.33772/biowallacea.v2i1.541

Abstract

The use of plants as traditional medicine has been known since long time ago by people in Lipu. This process has been inherited from one to next generation, nevertheless. nowdays, it has started to be extincted. So, it should be better to indentify the species of plant used as traditional medicine by people in Lipu. The research aims to investigate thekinds of plant which uses as traditional medicine. The part of plant used and how to process and the adventage of the plant. The research used explorative survey method. The result of the research there are 54 species from 31 family of plants used as traditional medicine by the people. The parts used of plant are leaf, stem/bark, root, flower, fruit, seed, tuber,rhizome and sap. The processing of the plants by refine, boil, squeeze, parch, pour boiling water, rasp and without processing. And the ability of the plants are can treat kinds of disease.
PENGHASILAN BIOPLASTIK OLEH ISOLAT INDIGENUS Bacillus sp. AMILOLITIK DENGAN SUBSTRAT PATI SUWEG (Amorphophallus campanulatus) Margino, Sebastian; Sari, Rarat Mulat; Martani, Erni
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
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Abstract

bioplastic that was synthesized by bacteria. Glucose as the main carbon source for PHBproduction but its cost was expensive, so we need to substitute with another substrate,including starch.The ability of amylolytic bacteria hydrolyzed starch into simple sugars andconverted to PHB can minimize the costs of PHB production. This research aim was to findout amylolitic bacterial isolates and produce PHB using the selected isolate. A total of 52bacteria were isolated using starch mediumfrom soil sample around Amorphophalluscampanulatus plant. Twenty eight among of them had amylase activity, and eleven among ofthem had amylase activity value equal or more than 2 point.The next selection was donebased on the specific amylase activity and found that five of those isolates had specificamylase activity more than 10 DUN/mg.Final selection was done based on PHB productionand found that isolate E5 had the high amylase activity and PHB production. PHB productionused isolate E5 in optimum conditions (inoculum concentration 10 %, elephant foot yamstarch 3 %, medium pH 7, temperature 30 °C, agitation 125 rpm, and 48 hour incubationtimeshowed that percentage of PHB increased from 9,5% to 14,5%. Characterization andidentification showed that isolate E5 closed to the genus Bacillus sp.Keywords : Amylolytic Bacillus sp., poly-β-hidroxybutyrate, elephant foot yam starch.
DISTRIBUSI SPASIAL LOGAM BERAT Pb PADA PERAIRAN TELUK KENDARI, SULAWESI TENGGARA Armid Armid
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 2, No 2 (2015): Keragaman dan Bioteknologi
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v2i2.1466

Abstract

Study on spatial distribution of heavy metal Pb in the coastal area of Kendari Bay has been carried out. Sampling was conducted at 8 stations along the bay. Determination of Pb concentrations was performed utilizing the Atomic Absorption Spectrophotometry (AAS). The results yielded Pb concentrations within the ranges of 0.01-0.025 ppm whereby the highest level was found at station #7 (Ferry Port) and #8 (Nusantara Port). Spatial analysis via Geographic Information System was accomplished by Inverse Distance Weight (IDW) interpolation method utilizing ArcView GIS 3.3 software for mapping 42 other stations along the Kendari Bay. The results showed that, by 50 stations in total, Pb levels was moderate in the middle part of the bay with average concentration of ~0.015 ppmKeywords : Spatial analysis, heavy metal, Pb, inverse distance weight, Kendari Bay.
PENGARUH PEMBERIAN MONOSODIUM GLUTAMAT TERHADAP STRUKTUR MIKROSKOPIS OVARIUM MENCIT (Mus musculus, L.) Wa Ode Harlis; Sitti Wirdhana Ahmad; Marwati Marwati
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 2, No 2 (2015): Keragaman dan Bioteknologi
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1074.272 KB) | DOI: 10.33772/biowallacea.v2i2.1476

Abstract

ABSTRACTThis study aims to determine the effect of MSG to the microscopic structure of the ovaries of mice. This study was a experimental study using a completely randomized design (CRD). A total of 16 female mice aged 3 month were devided into 4 groups namely P0 (Control), P1 (0,012 mg/gr BB), P2 (0,015 mg/gr BB) and P3 (0,018 mg/gr BB), MSG administration carried out of 14 days. On day 15 the animals were sacrificed and performed organ harvesting ovaries to make histological preparations. Observation were made by observing the histological structure of the ovaries , and count the number of primary follicles, secondary, tertiary, the korpus luteum and follicular atretic. Data were tested with ANOVA, and LSD (α=0,05%). The result showed the existence of structural damage in the form of a gap between the ovarian follicular granulosa cells and ovarium follicle diameter reduced with increasing doses of MSG given. MSG administration also decreases the average number of follicles in all treatment doses, but the mean of the highest drop in the number of follicles present in treatmen P3 that is, the number of primary follicles (2,50), secondary follicles (2,25), tertiary follicles (1,25) and follicular atretic (4,25), while the corpus luteum is not found.Keywords : Monosodium glutamate, Damaged Structure of The Ovaries, Mice (Mus musculus,L)

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