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Journal of Tropical Biodiversity and Biotechnology

jtbb
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Published by Universitas Gadjah Mada

ISSN : 25409573 EISSN : 25409581 DOI : -

Core Subject : Science, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.

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Articles 7 Documents

Search results for , issue "Vol 1, No 1 (2016): June" : 7 Documents clear

Keanekaragaman Serangga Sebagai Pakan Alami Katak Tegalan (Fejervarya limnocharis, Gravenhorst) pada Ekosistem Sawah di Desa Panggungharjo, Kecamatan Sewon, Kabupaten Bantul Farid Kuswantoro; R.C. Hidayat Soesilohadi
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

Paddy field was a dynamic and biodiversity rich ecosystems. Insect diversity in paddy field ecosystem was infected by paddy plant growth stages. Grass frog (Fejervarya limnocharis) ate insects as their natural prey. Insect population dynamics during vegetative, generative and reproductive stage of paddy plants infected F. limnocharis natural prey. This research aims were to study insect diversity and F. limnocharis insect prey diversity of vegetative, reproductive and generative paddy plant growth stages at Panggungharjo village, Sewon, Bantul. Insect sampling was conducted by direct survey method while F. limnocharis collection conducted by Visual Encounter Survey (VES) method. Ninety eight insect species from 51 different families and nine orders of insect were found. The orders were Coleoptera, Diptera, Hymenoptera, Odonata, Orthoptera, Dictyoptera, Dermaptera, Hemiptera and Lepidoptera. Stomachs analysis indicated F. limnocharis ate nine insect orders. The orders were Coleoptera, Diptera, Hymenoptera, Orthoptera, Dictyoptera, Dermaptera, Hemiptera, Lepidoptera and Isoptera. Most commonly insect prey in vegetative, reproductive and generative phase paddy growth stage were the members of the Order Coleoptera, Family Acrididae (Orthoptera) and Family Formicidae (Hymenoptera) respectively. This study concluded F. limnocharis main natural prey was the easily found and easily perceived insects.

Identifikasi Sifat dan Distribusi Sel Endokrin Ghrelin di Lambung Tikus (Rattus norvegicus): Studi Immunohis-Tokimia pada Kondisi Obesitas Teguh Budipitojo; Hevi Wihadmadyatami; Ganies Riza Aristya
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

Obesity is one of major nutritional problems in the world. Obesity is very dangerous, especially when concentrated in the abdomen, because it is closely linked to various diseases such as diabetes, hypertension, heart disease, which can to causing death. This study aims to identify the nature and distribution of ghrelin on gastric endocrine cells in the obese rat (Rattus norvegicus) by using immunohistochemical techniques. The results will strengthen the understanding of the role and function of ghrelin as an alternative therapeutic target on obesity. The research used gastric tissues of ten obese and control rats which were stained with avidin-biotin-peroxidase complex method of immunohistochemistry. The results showed the existence of two types of ghrelin-producing cells (open and closed types) on the gastric mucosa of control rats, and only one type of ghrelin producing cells (open type) in obese rats. The intensity of ghrelin immunoreactive positive cells was detected weak in obese rats, but very strong in control rats. Ghrelin endocrine cells mainly distributed in the basal part of the gastric mucosa of the fundus parts, with a very small number in obese rats, but highly abundant in control rats. This study confirmed the decrease of the ghrelin synthesis and secretion in obese rat (Rattus norvegicus) at the cellular level. The decrease of ghrelin synthesis is characterized by a reduction on the number of ghrelin producing cells, the disappearance of the close type of ghrelin producing cells, and the low activity of protein synthesis in the ghrelin producing cells. Ghrelin endocrine cells distributed mainly in the basal part of the gastric mucosa, especially in the fundus parts.

Keragaman Rubus di Gunung Kembang Kabupaten Wonosobo Jawa Tengah dan Potensi Pemanfaatannya Ratna Susandarini
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

Rubus is a member of Rosaceae family where its natural habitat is in open areas of lowland and upland forests. Rubus has been reported found in Mount Kembang which is situated in Wonosobo, Central Java, but the number of species was unclear. The objective of this study was to investigate the Rubus diversity in M. Kembang and its uses by local people. Sample collecting was carried out using explorative methods on the slopes of M. Kembang. Uses information was obtained by interviewing the local people. The data were analyzed descriptively to provide species description along with their uses and potential in biological research. The result showed that there were four species of Rubus in M. Kembang, R. rosifolius J.E.Smith, R. chrysophyllus Reinw. Ex Miq., R. fraxinifolius Poir., and R. moluccanus L. with altitudinal distribution ranging from 945 m to 1,387 m above sea level. Among these four species R. rosifolius is the well-known one, that people sell the fruits in its fruiting season in the local market. Based on the literature some Rubus species in other areas in Java have potential as fresh fruits, source for making fruit jam, traditional herbal drink to cure diarrhoea and dysentery, or as ornamental plants. A few studies showed that the fruit extract of R. rosifolius has potential for natural dye in cosmetic products.

Micropropagation of Mini Orchid Hybrid Phalaenopsis “Sogo Vivien” Exsyupransia Mursyanti; Aziz Purwantoro; Sukarti Moeljopawiro; Endang Semiarti
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

Phalaenopsis “Sogo Vivien” is an orchid hybrid with mini size plant body, and exhibits numerous beautiful pink flowers, that is ideal as ornamental pot plant. Some plants of this orchid exhibit variegated leaves that improve the beauty of the plant, not only because of the flower but also as attracted leaves. This orchid has high economical value, but mass propagation of this orchid has not established yet. An effective method to propagate both the normal and variegated plants is worth to be generated. The objective of this research was to produce a large number of P. “Sogo Vivien” plants, including the variegated plants. The method used seeds from self pollinating variegated plant, and flower stalk nodes. The seeds were sown on three various medium: VW, NP and MS, and flower stalk nodes were planted on VW + BA 10 mg l-1 + active carbon. The results showed that the best medium for in vitro culture of P. “Sogo Vivien” was NP medium, in which all seeds could grew into plantlets. Most plantlets emerged from the seeds were non variegated, only one plantlet out of 1344 seeds was variegated (0.007%). Although all emerged plantlets from flower stalk exhibited variegated leaves. Particularly, the plantlets arised from the second and third basal nodes of flower stalk showed the highest growth rate than that from the other nodes. Histological analysis showed that at 11-13 days after shoot segment plantation on NP medium, the shape of apical cells in the nodes was changed, then followed by the change of cell shape in the basal part of the nodes, produced bipolar pattern, then gradually developed into shoot. These results suggest that mass propagation could be achieved using seed culture, but to get the variegated phenotypes, the second and third nodes of flower stalk from variegated plant were the best explants to be used.

Karyotype Ikan Mas (Cyprinus carpio Linnaeus 1758) Majalaya Tuty Arisuryanti; Anjar Tri Wibowo
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

Common carp (Cyprinus carpio Linnaeus 1758) is grouped into Family Cyprinidae and Order Cypriniformes. One of Indonesian common carp which is commonly domesticated in Indonesia to fulfil consumer needs of animal protein is “Majalaya”. Even though the common carp “Majalaya” is extensively domesticated to meet market demands, any comprehensive study on the fish chromosomes has not been investigated. Chromosome study on the common carp “Majalaya” is quite important related to breeding program including certification process. The objective of this study was to study karyotype of the common carp “Majalaya” collected from UKBAT Cangkringan, Yogyakarta. Chromosome preparation of the common carp “Majalaya” investigated in this study was a splash method with blood cell cultures. The results revealed that the common carp “Majalaya” has diploid chromosome number (2n) = 98 classified as 12 (6 pairs) metacentric chromosomes and 86 (43 pairs) telocentric chromosomes displaying karyotype formula 2n = 2x = 98 = 12 m + 86 t. However, sex chromosomes of the common carp “Majalaya” could not be identified in this study. In addition, the variation of total length chromosomes of the fish is between 1,052 μm and 3,299 μm. On the basis of R value (3,079 ± 0.093), the common carp “Majalaya” has chromosome variation size

Keanekaragaman Jenis Ikan di Sepanjang Sungai Boyong – Code Propinsi Daerah Istimewa Yogyakarta Tri Trijoko; Donan Satria Yudha; Rury Eprilurahman; Setiawan Silva Pambudi
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

The diversity of freshwater fishes which inhabit in the river of Daerah Istimewa Yogyakarta is not yet well documented. Complete documentation is needed as starting point and continuous research on the fish diversity in DIY. Boyong-Code River flows across the DIY, and it upstream is located on the hillside of Merapi volcano. The Code River upstream is called Boyong River. The research was aimed to acquire data about the diversity of fish fauna along the Boyong-Code River in the DIY. Further, the research purpose is to know which species are rare, potential for aquaculture, and introductive. Samples are taken along the Boyong-Code River starting from upstream to downstream. Samples were collected using Purposive Random Sampling methods with fishnets. Sampling area generally divided into three location i.e., upstream, middle-stream and downstream. Species diversity of fish in the Boyong-Code River is consisted of 24 species, with 5 introductive species. There are eleven native fish species which are potential for cultivation (aquaculture), i.e.: Barbodes binotatus, Mystacoleucus obtusirostris, Rasbora lateristriata, Rasbora argyrotaenia, Barbonymus balleroides, Osteochilus vittatus, Hampala macrolepidota, Anabas testudineus, Channa striata, Clarias leiacanthus and Clarias batrachus. The Boyong-Code River is a decent habitat for fishes. Many introduced fishes starting to invade the Boyong-Code River intentionally or unintentionally by human

Populasi dan Sebaran Jenis Moluska Dilindungi di Perairan Selat Lembeh, Kota Bitung, Sulawesi Utara Ucu Yanu Arbi
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

Exploration of protected mollusks has been carried out at Lembeh Strait, Bitung, North Sulawesi for 30 months in 2007 until 2009. Protected mollusks can be found at Lembeh Strait and the population may decline because of environmental stress. The aim of this study is to determine populations of protected mollusc at Lembeh Strait waters. Observations were made at 15 locations with free collection methods. In this location, 175 individuals comprising 12 species of protected mollusks were collected. Tridacna maxima was the most extensive distribution in the research location. From the research, Lembeh Strait was advised to obtain marine protected areas.

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