cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota malang,
Jawa timur
INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 15 Documents
 1   2 
Search results for , issue "Vol. 12 No. 3 (2022)" : 15 Documents clear
In Silico Study of Eugenol and trans-Caryophyllene also Clove Oil Fumigant Toxicity on Tribolium castaneum: In Silico Study and Fumigant Toxicity of Clove Oil Silvi Ikawati; Toto Himawan; Abdul Latief Abadi; Hagus Tarno; Alvan Fajarudin
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.07

Abstract

Alternative storage pest control that is more environmentally friendly than the use of synthetic chemical pesticides is to use botanical pesticides from plant essential oils, including clove (Syzygium aromaticum) which contains the main compounds eugenol and trans-caryophyllene. To study the various mechanisms of action of essential oils as botanical insecticides could use in silico approach through molecular docking. This study aims to predict the dominant binding mode(s) of a ligand with a protein of a known three-dimensional structure through docking. Then tested its fumigant activity on Tribolium castaneum. The docking results showed that the trans-caryophyllene and eugenol compounds had a more stable bond strength in the acetylcholinesterase enzyme T. castaneum than the control compound linalool. In addition, there is a synergy between eugenol and trans-caryophyllene when the two compounds interact with acetylcholinesterase. These results can be used as prediction material that trans-caryophyllene and eugenol have potential as protein acetylcholinesterase inhibitors of T. castaneum. After being tested in the laboratory, clove oil which contains two main compounds namely eugenol and trans-caryophylene has the potential to control T. castaneum with an LC50 value of 5,227 μL/L air. Keywords: Botanical insecticide, Caryophyllene, Docking, Eugenol, Fumigant,Syzygium aromaticum
Exploration of the Potential of Local Plants of Melastoma malabatchricum Fruit for Food Fortification: Potential of Local Plants of M. malabatchricum Fruit for Food Fortification fathul zannah; Kamaliah; Pramudiyanti; Ayatusaadah; Nurul Hadayati
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.06

Abstract

Endurance can be increased by maintaining nutritional intake, one of which is by consuming local plants such as Melastoma malabatchricum  Fruit. This study aims to explore the nutritional content of Melastoma malabatchricum  fresh fruit, flour and processed products from the flour. This research was conducted at the Baristand Laboratory of the Banjar Baru Industrial Center, South Kalimantan. The results showed that flour and processed products from Melastoma malabatchricum  flour had higher nutritional content than fresh Melastoma malabatchricum  fruit. Nutritional content of Melastoma malabatchricum  flour are cal, ca, Fe, Water, Ash, Fat, Protein and Carbohydrate. These results indicate that Melastoma malabatchricum  has the potential to be developed as an ingredient for food fortification.
Factors Related to Acceptance of Covid-19 Vaccine among Hemodialysis Patients in Indonesia: Factors Related to Acceptance of Covid-19 Vaccine among Hemodialysis Patients Langgeng Perdhana; Suryani Yuliyanti; Shofa Chasani; Muchlis Achsan Udji Sofro
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.12

Abstract

Hemodialysis patients are at risk of being infected with Corona Virus Disease 2019 (Covid-19). On the other hand. End-Stage Kidney Disease (ESKD) plays a role as a predictor factor of severe Covid-19 infection. There is no data about factors that affect Covid-19 vaccine acceptance among hemodialysis patients in Indonesia. This study’s goal is to determine the factors related to Covid-19 vaccine acceptance among hemodialysis patients. A Cross-sectional study occurred in September 2021 in the hemodialysis unit at Roemani Muhammadiyah Hospital Semarang. Inclusion criteria include ESKD patients who underwent hemodialysis ≥3 months, hemodialysis frequency twice a week, communicable, and agree to join in this study. While patients with incomplete data. and being treated in the Covid-19 isolation ward are excluded from this study. Collected data analyzed using chi-square using Statistical Program for Social Science (SPSS) 18.0 version. From 87 respondents, 36 (41.4%) respondents have been vaccinated and 51 (58.6%) respondents have not been vaccinated. Hemodialysis patients with high educational level, elderly, and has undergone hemodialysis >18 months have higher acceptances of Covid-19 vaccine (57.1% and 33.9%; 60.9% and 34.4%; 51% and 38.5%, p-value 0.04; 0.027; 0.03. Prevalence Ratio (PR) = 1.7; 1.8; 1.8). While gender, marital status, and history of Covid-19 infection did not have a significant relationship to Covid-19 vaccine acceptance (p > 0.05). Educational level, elderly patients, and duration of hemodialysis have a higher acceptance of the Covid-19 vaccine among the hemodialysis population.
The Evaluation of Biochemical Parameters of Ethanolic Extract of Lepidium meyenii in Male Wistar Rats : Evaluation of Biochemical Parameters of Ethanolic Extract of Lepidium meyenii Hamdalat Folake Muritala; Habeebat Oyewesi; Tobiloba ogunlana; Loveth Ajakaiye; Kehinde Aina; Waleed Ibrahim; Fiyinfoluwa Patricia Ogunyinka; Halimat Aliyu; Clement Olatunbosun Bewaji
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.13

Abstract

The effects of ethanolic extract of Lepidium meyenii on some biochemical parameters were investigated. Forty male Wistar rats were grouped into five groups of eight animals each. Group 1 served as the control and received distilled water throughout the period of administration while groups 2, 3, 4 and 5 received 25, 50, 75 and 100 mg/kg body weight of ethanolic extract of L. meyenii respectively for seven and fourteen days. After seven days of administration, four animals from each of the groups were sacrificed and the rest were sacrificed after fourteen days, and biochemical alterations were monitored. The effects after the seven-day administration revealed that there was no significant (p > 0.05) difference in organ-body weight percentage, liver functional indices, kidney functional indices and the activity of some cytosolic and membrane bound enzymes studied but  following the fourteen-day administration, significant difference (p < 0.05) were observed with increase in organ-body weight percentage of selected organs, increase in serum activity of the enzymes: ALT, AST, ALP; and aberrant variation in the kidney functional indices studied at the 50, 75 and 100 mg/kg body weight showing selective toxicity of the extract. Inconclusive results were obtained in the analysis of the seminal parameters for both the seven- and fourteen-day administration while similar effects on hematological parameters were observed for both the seven- and fourteen-day administration. This study showed that the extract may be hepatotoxic and nephrotoxic when administered beyond seven days in male Wistar rats especially at 100 mg/kg body weight.
Epitope mapping of ORF3a Protein SARS-CoV-2 in Indonesia through computational study: Epitope mapping of ORF3a Protein SARS-CoV-2 in Indonesia Hartiyowidi Yuliawuri; Jeanne Elvia Christian
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.08

Abstract

In Indonesia, the vaccine for SARS-CoV-2 is still being developed. ORF3a protein of SARS-CoV-2 could be a potential peptide to be used in the vaccine, therapeutic antibodies, and diagnostic kit development. We used the computational approach in designing some potential epitopes against the ORF3a protein of the virus. The reference sequences of ORF3a SARS-CoV-2 were retrieved from GISAID. In our previous study, we found 203 non-synonymous mutations from 3,751 samples in Indonesia. We categorized and compared the variation in this protein with reference sequences. To predict B cell epitopes, we used Immune Epitope Database (IEDB) and VaxiJen v.2.0 as analysis resources. We found two epitopes 177SPISEHDY184 and 74SKGVHFVCNLLLLFVTVYSHLLLVAAG100 that indicate antigen. Our study showed these predicted peptides could be used as a reference for the SARS-CoV-2 vaccine, therapeutic antibodies, and diagnostic development. This approach needs further studies to understand the functionality of the predicted proteins against SARS-CoV-2 that circulated especially in Indonesia.
Cellulolytic Bacteria Associated with Gut of Longhorn Beetle, Prionomma bigibbosum (Coleoptera: Cerambycidae): an Electron Microscopic Study: Cellulolytic Bacteria Associated with Gut of Prionomma bigibbosum SUMITA BISWAS; DIBYENDU PAUL; ATANU BHATTACHARJEE
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.09

Abstract

Adult Cerambycid (sub-family Prioninae) beetles are non-feeders. The present study aims to investigate and evaluate the gastrointestinal microbiota in long horn beetle Prionomma (Ancyloprotus) bigibbosum White (1853) (Coleoptera: Crambycidae: Prioninae: Prionini) gut. Scanning electron micrograph revealed the presence of abundant bacteria firmly attached to hindgut. The gut flora were isolated and screened on Carboxymethylcellulose (CMC) agar medium using CMC as sole carbon source. The cellulolytic activity was measured both qualitatively and quantitatively. Cellulolytic efficiency was assessed by DNS method. Potent cellulose degrader bacterial isolate was subjected to phenotypic and genotypic characterization. A Gram positive, non-motile, oxidase positive coccoid isolate designated as PBI9 was found to be efficient cellulose degrader. Based on 16S rRNA gene analysis, the isolate was found to be most closely related to Mammaliicoccus fleurettii, Mammaliicoccus stepanovicii and Mammaliicoccus lentus (99.24%, 99.17% and 99.17% respectively) and was identified as Mammaliicoccus  sciuri (99.86% similarity) (NCBI Accession number MZ351443). This appears to be first study undertaking SEM of gut microbiota of longhorn beetle, P. bigibbosum and to report the P. bigibbosum gut as a novel source of cellulolytic bacteria.
In Silico Analysis and 3D Structure Prediction of Putative UDP-Glycosyltransferase 76G1 Protein in Stevia rebaudiana MS007: In Silico Analysis and 3D Structure Prediction of Putative UDP-Glycosyltransferase 76G1 Nor Iswani Mokthar; Muhammad Amirul Husni Samsulrizal; Afiqah Ramatullah Khan; Zarina Zainuddin; Tamil Chelvan Meenakshi Sundram; Nik Yusnoraini Yusof; Nurul Hidayah Samsulrizal
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.11

Abstract

Stevia rebaudiana is a plant of the Asteraceae family that is used as a natural sweetener. Stevia has been shown to be safe for human consumption and has been utilised as a sweetener substitute for diabetic and obese people. In this study, the structure and gene content involved in the synthesis of putative UDP-glycosyltransferase 76G1 (UGT76G1) protein in S. rebaudiana MS007 was analyzed using an in silico method. Homologous search using blastP revealed the highest percentage of identity, score, and E-value for UDP-glycosyltransferase 76G1-like of Helianthus annuus (ID: XP_021973845.1). The presence of IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase entry, which is available at locations 89bp to 246bp, was also verified by the protein family search using InterPro. MEGA-X software was used to construct a molecular phylogeny study, which revealed that this protein belongs to the Asteraceae protein family. To predict the primary, secondary, and tertiary protein structures of the putative UGT76G1 protein, the ProtParam, ExPasy, PSIPRED, and Phyre2 programs were implemented. The putative UGT76G1 protein’s tertiary structure prediction was given a score of 100.0% confidence by the single highest scoring template and a coverage of 98% with the dimension of the model being (Å) of X: 52.453, Y: 61.270, and Z: 48.102. The UGT76G1model fulfilled the quality standards and was approved for further analysis after validation performed by PROCHECK, VERIFY3D, and ERRAT. Thus, the findings of this work have contributed to a better knowledge of putative UDP-glycosyltransferase 76G1 features and target recognition processes, which will lead to a better information of protein-protein interaction in S. rebaudiana MS007.
Effective control of Alphitobius diaperinus using natural bioinsecticides : Effective control of Alphitobius diaperinus using natural bioinsecticides Niken Subekti; Shinta Hedy Cahyaningrum; Sonika Maulana
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.01

Abstract

Pest insects known as darkling beetles (Alphitobius diaperinus) are common in poultry farms. These pests can develop into a variety of disease vectors, causing damage to chicken coops, decreased chick performance, and financial losses to farmers. In Indonesia, synthetic insecticides are still used for pest control, which is detrimental to non-target creatures and the environment. The purpose of this study is to analyze the effectiveness of biopesticides from cinnamon bark extract (Cinnamomum aromaticum), nutmeg seeds (Myristica fragrans), lavender flowers (Lavandula angustifolia), and lime peel (Citrus aurantifolia), as well as synthetic insecticides alpha-cypermethrin against A. diaperinus larvae and imago. Bioinsecticide levels are 2.5%, 5%, 7.5%, and 10%, while alpha-cypermethrin levels are 1%, 4%, 7%, and 10%, respectively, based on LC50 values. The analysis was repeated three times and the treatment was carried out every two days for a total of 14 days. The data were analyzed with paired sample t-tests to determine the average difference in each treatment when there was an average difference and calculated N-Gain score to determine the effectiveness of biopesticides and synthetic insecticides. The average value of N-Gain essential oil from cinnamon bark extract was successful in causing A.diaperinus death in the imago stage (81.14) and the highest larvae (80) of other test solutions. Cinnamon bark extract and nutmeg seed extract are effective in imago (95.14) and larvae (78.91), respectively, and can be used as a natural biopesticide to replace synthetic insecticides in the control of A.diaperinus in imago and larvae stages.
In Vitro Digestibility Study: Evaluating Plant Proteins Digestibility in Anabas testudineus and Channa punctata: In vitro digestibility study in A. testudineus & C. punctata Rita Devi; Monika Basumatary; Bichitra Narzary; Heikham Dayami; Sanraja Muchahary; Bronson Kumar Khangembam
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.03

Abstract

Protein is the most important component of any fish feed for its role in growth, especially during the larval stages, and its high cost. Fish meal continues to be a major source of protein in fish feed production. But its supply cannot keep pace with ever-expanding aquaculture production, leading to its high cost. Plants are being considered as potential replacements in the search for new alternatives to fish meals. But their application depends significantly on their digestibility in target species. The present study aims to determine the protein content of four locally available plants Moringa oleifera, Ipomoea aquatica, Lemna minor and Salvinia natans, and test their digestibility in vitro by using the pH drop method in two important food fish Anabas testudineus and Channa punctata, of Assam, India. The crude protein in all plants ranged from 19-29%, and the highest crude protein was observed in Lemna minor (29.9 ± 2.34%). The in vitro digestibility was estimated by calculating the RPD% (relative protein digestibility) using casein as a standard reference. Digestibility of the plant proteins exhibited species-specific variations. The RPD% ranged from 50.39% to 75.39% in A. testudineus, and 41.38% to 54.02% in C. punctata compared to that of  casein (100%). The highest RPD% was observed in I. aquatica (75.39%) for A. testudineus, and the lowest (50.39%) in L. minor whereas, in C. punctata, the highest RPD% was observed in L. minor (54.02%) and the lowest in I. aquatica (41.38%). The digestibility of all plant proteins was comparatively higher in A. testudineus than in C. punctata. Our results indicate that I. aquatica and L. minor may be a suitable replacement for animal protein in the diet of A. testudineus and C. punctata, respectively, because of their good protein content and high digestibility. Moringa may be considered for utilization in the fish feed as it recorded good protein and digestibility. This information may be useful in developing a cost-effective, plant-based protein diet for the two fish species for their mass production.
Reconstructing Phylogenies of Alocasia spp. (Araceae) Distributed in Indonesia for Conservation Prioritization: Reconstructing phylogenies of Alocasia spp. (Araceae) Arief Priyadi; Ni Putu Sri Asih; Ina Erlinawati
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.04

Abstract

Phylogenetic analysis is beneficial to plant conservation prioritization. Conservation does not only deal with species but also their evolutionary potential. A phylogenetic tree is usually reconstructed by using the alignment of DNA sequences. However,  with the availability of megatrees, subsetting with a list of species of concern is possible. Here we reconstructed a list of 53 Alocasia spp. (Araceae) from Indonesia and adjacent regions using available megatrees and publicly available DNA sequences in the GenBank. The result showed placed and unplaced Alocasia spp. on the terminal nodes of reconstructed phylogenetic trees. The unplaced species show that publicly available DNA sequences are not yet available, therefore should be prioritized for sequencing. The placed species in the phylogeny could be used for conservation prioritization of these Alocasia spp.

Page 1 of 2 | Total Record : 15

 1   2 

Filter by Year

2022 2022


Reset
Filter By Issues
All Issue Vol. 13 No. 3 (2023) Vol. 13 No. 2 (2023) Vol. 13 No. 1 (2023) Vol. 12 No. 3 (2022) Vol. 12 No. 2 (2022) Vol. 12 No. 1 (2022) Vol 11, No 3 (2021) Vol. 11 No. 3 (2021) Vol. 11 No. 2 (2021) Vol 11, No 2 (2021) Vol. 11 No. 1 (2021) Vol 11, No 1 (2021) Vol 10, No 3 (2020) Vol. 10 No. 3 (2020) Vol 10, No 2 (2020) Vol. 10 No. 2 (2020) Vol 10, No 1 (2020) Vol. 10 No. 1 (2020) Vol 9, No 3 (2019) Vol. 9 No. 3 (2019) Vol. 9 No. 2 (2019) Vol 9, No 2 (2019) Vol. 9 No. 1 (2019) Vol 9, No 1 (2019) Vol. 8 No. 3 (2018) Vol 8, No 3 (2018) Vol 8, No 2 (2018) Vol. 8 No. 2 (2018) Vol 8, No 1 (2018) Vol. 8 No. 1 (2018) Vol 7, No 3 (2017) Vol. 7 No. 3 (2017) Vol 7, No 2 (2017) Vol. 7 No. 2 (2017) Vol 7, No 1 (2017) Vol. 7 No. 1 (2017) Vol 6, No 3 (2016) Vol. 6 No. 3 (2016) Vol 6, No 2 (2016) Vol. 6 No. 2 (2016) Vol. 6 No. 1 (2016) Vol 6, No 1 (2016) Vol. 5 No. 3 (2015) Vol 5, No 3 (2015) Vol 5, No 2 (2015) Vol. 5 No. 2 (2015) Vol 5, No 1 (2015) Vol. 5 No. 1 (2015) Vol. 4 No. 3 (2014) Vol 4, No 3 (2014) Vol 4, No 2 (2014) Vol. 4 No. 2 (2014) Vol. 4 No. 1 (2014) Vol 4, No 1 (2014) Vol. 3 No. 3 (2013) Vol 3, No 3 (2013) Vol. 3 No. 2 (2013) Vol 3, No 2 (2013) Vol 3, No 1 (2013) Vol. 3 No. 1 (2013) Vol. 2 No. 3 (2012) Vol 2, No 3 (2012) Vol. 2 No. 2 (2012) Vol 2, No 2 (2012) Vol 2, No 1 (2012) Vol. 2 No. 1 (2012) Vol. 1 No. 2 (2011) Vol 1, No 2 (2011) Vol 1, No 1 (2010) Vol. 1 No. 1 (2010) More Issue