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International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 11 Documents
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Search results for , issue "Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY" : 11 Documents clear
BONE FLOUR FROM SUCKERMOUTH FISH (Pterygoplichthys pardalis) AND TILAPIA FISH (Oreochromis mossambicus) Riris Lindiawati Puspitasari; Femilda Khavidhar; Kintan Ayu Gustiananda; Muhammad Alwan; Puspitha Cahaya Ayu Widyanto; Siti Anisah; Ainul Haq
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2021.v09.i01.p05

Abstract

Fish is often used as a protein source in food. Fish has good nutritional content for the body such as protein, vitamin, mineral, and unsaturated fatty acid. Pterygoplichthys pardalis and Oreochromis mossambicus are some of the local fishes that are frequently found in a river in Indonesia, also Asia. People use their meat as food. Unfortunately, people do not use fishbones. In this research, we processed the fishbone into flour which could be used as food or supplement. Fishes were collected from the breeder in January to May 2019. Bone processing methods carried out by separation of fish meat and bone, continued with bone flour processing using oven and grinder. According to the proximate analysis revealed that P. pardalis bone flour contained 25.047% protein, 10.478% fat, 0.656% water, 59.463% ash, and 4.353% carbohydrate. O. mossambicus also contained 36.929% protein, 11.942% fat, 2.575% water, 45.721% ash, and 2.833% carbohydrate. From these results, protein in P. pardalis gave a higher value than O. mossambicus. Bone flour from both fishes showed a smooth texture and whitish color. Bone flour from fish has good prospects to be developed to get complete analysis in food technology. Bone flour from suckermouth fish played important role in aquaculture and supported the river biodiversity.
Back Cover IJBB Vol. 9 No. 1 September 2021 Putu Perdana Kusuma Wiguna
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Back Cover IJBB Vol. 9 No. 1 September 2021
THE POTENCY OF CHITOSAN AS AN ELICITOR ON ANTIBACTERIAL ACTIVITY OF Streptomyces sp. GMR-22 AGAINST HISTAMINE-PRODUCING BACTERIA Mohamad Aji Ikhrami; Jaka Widada; Indun Dewi Puspita; Masagus Muhammad Prima Putra
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2021.v09.i01.p01

Abstract

Streptomyces is a Gram-positive bacteria that produces the largest secondary metabolite compounds. The results of whole-genome sequence analysis showed that Streptomyces can carry more than 30 Biosynthetic Gene Clusters (BGC) encoding secondary metabolites that have the potential to be explored in the exploration for new bioactive compounds. However, not all BGC can be expressed in the laboratory scale and requires a specific activation method. This study aims to explore the potential of chitosan as an elicitor compound to activate and or increase the antibacterial activity of Streptomyces sp. GMR-22 was tested against histamine-producing bacteria (HPB) Morganella morganii TK7 and Citrobacter freundii CK1. Chitosan was added to the fermentation medium with the final concentration of 250, 500, and 750 µg/ml while without the addition of chitosan used as control. Fermentation was carried out for 10 days at room temperature, with constant agitation 200 rpm. The supernatant was separated by centrifugation at 3500 rpm for 15 minutes, then fractionation with ethyl acetate, concentrated by vacuum rotary evaporator, and freeze-dried. The test for antibacterial activity was carried out by the microdilution method with an extract concentration of 100 mg/ml. The test results of the microdilution method showed that the addition of chitosan successfully increases the antibacterial activity with the highest activity shown by the water fraction of 250 µg/ml addition of chitosan which effective in inhibiting the growth of Morganella morganii TK7 and Citrobacter freundii CK1 by 97,29% and 97,92% respectively.
ISOLATION AND ABILITY TEST OF PHENOL-DEGRADING BACTERIA FROM THE LIQUID WASTE IN BHAYANGKARA HOSPITAL, DENPASAR CITY, BALI, INDONESIA Shara Yulita Harianja; I Gede Putu Wirawan; Made Sritamin
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2021.v09.i01.p06

Abstract

Phenol is an organic compound that is toxic to humans and the environment. This compound was contained in the hospital liquid waste and petrochemical-based industrial sectors. This study aims to obtain hydrocarbonoclastic bacterial isolates that act as biodegradation agent in waste handling. Samples obtained from the biofilter tank of liquid waste treatment Bhayangkara Hospital Denpasar,Bali, Indonesia then carried out isolation and inoculation of bacteria in selective media NA (Nutrient Agar) containing Phenol. The bacteria obtained was performed the viability test on the media with various Phenol concentration levels, obtained three isolates of bacteria capable of growing to the concentration Phenol 500 mg / L, i.e. isolates IB2, IB5, and IB10. Therefore, it was performed the degradation ability and rate test on those three isolates. After 72 hours of incubation periods, the change of Phenol concentrations was analyzed using the folinciocalteau method and examined using the spectrophotometer with 660 nm of wavelength. Based on the analysis of final phenol content, the IB2 isolate has the highest degradation capability in the concentration of 600 mg/L (153,4 mg) with a degradation rate of 2,13 mg/h. IB5 isolates have the highest degradation capability in the concentrations of 700 mg/L (109,45 mg) with a degradation rate of 1,52 mg/h. Meanwhile, IB10 isolates show the lowest ability and degradation rate in both concentrations. Through morphological identification and Gram stain, three isolates belong to the Gram-negative rod-shaped bacteria, which has similarities with the Pseudomonas genus.
MICROSCOPIC IDENTIFICATION OF VESICULAR ARBUSCULAR MYCORRHIZAE (VAM) ASSOCIATED WITH ORCHID Cymbidium sp. Gresia Eldina; I Gede Putu Wirawan; Made Sritamin
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2021.v09.i01.p02

Abstract

Cymbidium sp. is one of the most popular orchid desired in public, it means high productivity is needed in its fulfillment. One of the obstacle of Cymbidium sp. orchid in order to fullfilment of productivities are the stress and orchid’s seed size which is very small. Vesicular Arbuscular Mycorrhizae is a symbiont fungus that can help absorption of nutrients in stress phase. VAM help Cymbidium sp. to maintenance and increase the productivity. This research aimed to determined VAM species and colonization in rhizospher of Cymbidium sp. orchid. This research has been conducted from November 2020 untill April 2021. Spore isolation was conducted by using wet shievings method, meanwhile VAM colonization was conducted by using roots staining techniques. The result showed there were 4 genus consisted by 6 different type of spores which has been found. There was Glomus (2 types), Dentiscutata (2 types), Acaulospora (1 type), and Gigaspora (1 type). Colonization of VAM in sample plant has been known by presence of VAM special structure, there were vesicular and internal hyphae.
Cover IJBB Vol. 9 No. 1 September 2021 Putu Perdana Kusuma Wiguna
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Cover IJBB Vol. 9 No. 1 September 2021
IN VIVO ANTI-INFLAMMATORY ACTIVITY OF DADAP LEAVES (Erythrina subumbrans (Hassk.) Merr) Ni Luh Kade Arman Anita Dewi; Ni Luh Sintya; Maria Malida Vernandes Sasadara; Erna Cahyaningsih; Putu Era Sandhi Kusuma Yuda; Puguh Santoso
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2021.v09.i01.p03

Abstract

Leguminosae is widely known for its anti-inflammatory properties due to the presence of several beneficial phytochemicals, including alkaloids, flavonoids, steroids, and saponins. Dadap (Erythrina subumbrans (Hassk.) Merr), a Leguminosae member, has empirically been used to treat inflammation. This research aimed to prove the anti-inflammatory effect of Dadap leaves scientifically. Dadap leaves were macerated in 96% ethanol to produced crude ethanol extract, followed by in vivo anti-inflammatory assay with the paw edema method. Three different doses of extract: 200, 300, and 400mg/kg BW, were administrated to Wistar rats to observed its ability in reducing carrageenan-induced inflammatory. All extract doses produced similar anti-inflammatory activity as compared to diclofenac sodium as a positive control. There is no significant difference among the three different doses. The results indicated that all treatment doses produced anti-inflammatory activity, but 200mg/kg BW administration was most efficient.
Editorial Board IJBB Vol. 9 No. 1 September 2021 Putu Perdana Kusuma Wiguna
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Editorial Board IJBB Vol. 9 No. 1 September 2021
MORPHOLOGICAL IDENTIFICATION OF BACTERIA ISOLATED FROM MUSHROOM BAGLOG AND ITS EFFECT ON SHIITAKE (Lentinula edodes (Berk.) Pegler) AND REISHI (Ganoderma lucidum (Curtis) P. Karst) MYCELIAL GROWTH Ni Kadek Emi Sintha Dewi; I Gede Putu Wirawan; Made Sritamin
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2021.v09.i01.p04

Abstract

Reishi (Ganoderma lucidum (Curtis) P. Karst) and shiitake (Lentinula edodes (Berk.) Pegler) mushrooms have high economic value and contain bioactive metabolites that provide heatlh benefits. Shiitake mushrooms have a delightfully unique taste as an edible mushroom. Mushroom production can be increased by utilizing microbes known as MGPB (Mushroom Growth Promoting Bacteria). This study aimed to determine the presence of bacteria associated with shiitake and reishi mushrooms in the baglog and determine how these bacteria influence the growth of shiitake and reishi mycelium. Bacteria was isolated from the mushroom baglog then were purified and centrifuged to obtain the cell-free extract. Furthermore, the bacterial cell-free extracts were poured on PDA media, then shiitake and reishi mycelium were inoculated to test the effect of bacteria isolates on fungal growth. The results showed that three isolates have the potential as MGPB in reishi mushroom, i.e., isolate S1C, S1D, and S2A. Reishi mycelium grown on isolate S2A achieved the fastest maximum growth on day 6th. In shiitake mushroom, it was seen that all isolates showed a negative effect on mycelium growth. Shiitake mycelium in isolate S1C, S1D, S2A, and S2B were seen to form a clear zone on PDA medium.
Table of Content IJBB Vol. 9 No. 1 September 2021 Putu Perdana Kusuma Wiguna
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Table of Content IJBB Vol. 9 No. 1 September 2021

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