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INDONESIA
Indonesia Chimica Acta
Published by Universitas Hasanuddin
ISSN : 2085014X     EISSN : 26556049     DOI : -
Core Subject : Engineering,
Chemical Engineering, Chemistry & Bioengineering
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) is a peer-reviewed research journal that is devoted to the dissemination of new and original knowledge in all branches of chemistry. The result of research and development in the fields of chemistry in both experimental and theory/ computation, chemical-based technological innovations, and chemical applications in industrial fields. The journal publishes original research articles or review articles in organic chemistry, inorganic chemistry, analytical chemistry, physical chemistry, biochemistry, and environmental chemistry.
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Volume 1 No 1 - December 2008" : 5 Documents clear
Isolasi Dan Karakterisasi α-amilase Isolat Bakteri Amilolitik Asidofilik Dari Taman Nasional Rawa Aopa Watumaohai Sapto Raharjo; Ardiansyah Ardiansyah; Agus Chahyadi
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 1 No 1 - December 2008
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v1i1.2451

Abstract

Isolation and characterization α-amylase acidophilic amylolytic bacteria isolate from Rawa Aopa Watumohai National Park has been carried out. The aims of these studies are to know the characteristics of acidophilic amylolytic bacteria isolate, the optimum concentration of substrate and temperature α-amylase activity yielded, and specific activity in each enzyme purification steps. Activity of α-amylase was measured with DNS (Dinitrosalisilic acidI) method at λ 550 nm and the rate of protein enzyme was measured with Biuret method at λ 540 nm. The content of this research were obtained the isolate bacteria TR 1 which higher amylolytic activity is the colonies are pale-yellow with convexelevated, undulate, bacill sel, and negative Gram. The maximum activity and protein content obtained with (NH4)2SO4 extracted at 60% (w/v). The specific activity of α-amylase in crude extract was 0.038 U/mg enzyme, after extracted with (NH4)2SO4 60% (w/v) was 0.146 U/mg enzyme with purification fold 3.8, and after dialysis was 0.255 U/mg enzyme with purification fold 3.8, respectively. The optimum concentration of substrate (amylose) was 1.25% (w/v) and the optimum incubation temperature was 45oC.Keywords : Rawa Aopa Watumohai National Park, acidophilic amylolytic bacteria, α-amilase
Hubungan Sifat Reologis Adonan Terhadap Karakteristik Sensorik Produk Makanan Tepung Komposit Terigu-Sorgum Muhammad Zakir
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 1 No 1 - December 2008
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v1i1.2445

Abstract

Rheological characteristics of wheat-sorghum flour doughs has been investigated. The rheological properties of wheat dough were observed in the Laboratory of Quality control, PT Berdikari Sari Utama Flour Mills, Makassar. Organolepttic Test on bread made of the dough was conducted in the Laboratory of Food Processing and Chemistry, Research Institute of Cereals, Maros. Sorghum flour replaced wheat flour in the dough at substitution levels of 0, 10, 20, 30, 40 and 50%, respectively. Dough rheology was studied by extensigraph method. An interesting thing on the substitution level can be pointed out from the extensigram characteristics of the composite flour dough. Based on the R’5/E and Rm values, dough with similar baking characteristics to wheat flour dough was still obtained from composite flour until 20% substitution. The organoleptic test on the bread in terms of colour, taste, flavour and texture showed that substitution level at 20% level produced bread closely similar to wheat bread.Key words: Rheology, dough, flour composite, organoleptic, sensoric perception.
Utilization of Clay as Adsorbent Laboratory Waste Catherina M.Bijang; Ivonne Telussa
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 1 No 1 - December 2008
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v1i1.2446

Abstract

Treatment for laboratorium waste need to carry out for prevented environment pollution if accumulation occured in the world. Natural resources available in maluku like clay can used to treat heavy metal Pb and Cu wastes. This adsorben were clay from Ouw village in Saparua,Maluku. The clay were brown and black. Each clay were activated with soaked in sulfuric acid for 5 and 10 hours. The result showed that the black clay with activated for 5 hours in sulfuric acid had the best adsorb.This clay more effective for adsorp Pb2+ ion compare with Cu2+ ion.Keywords : clay, adsorben, heavy metal.
Studi Bioaktivitas Antibakteri Fraksi Protein Hidroid Aglaophenia cupressina Dari Pulau Lae-Lae Sulawesi Selatan Nur Umriani; Abdul Rauf Patong; Ahyar Ahmad
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 1 No 1 - December 2008
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v1i1.2447

Abstract

This research aimed to know the activity of bioactive protein, extracted from Hydroid (Aglaophenia cuppressina), in inhibiting the growth of pathogenic bacteria. Samples were taken from Lae-lae Island in South Sulawesi. Methods used were the Lowry method for determining the protein concentration and the agar diffusion method  for testing the antibacterial activity. Extraction of Hydroid was conducted by making use of buffer solution, (0,1 M Tris-HCl of pH 8.3, 2 M NaCl, 0.01 M CaCl, 1% β-mercaptoethanol, and 0.5% Triton X-100). Protein was purified by ammonium sulfate precipitation at 20%, 40%, 60% and 80% saturation followed by dialysis. Results showed that the protein fraction of Aglaophenia cuppressina before dialysis with 40 % ammonium sulfate saturation had the highest antibacterial activity to Staphylococcus aureus and Escherichia coli with inhibition zones of  14.58 and 18.05 mm, respectively. In addition, the protein fraction of Aglaophenia cuppressina after dialysis with 40 % saturation also had the highest antibacterial activity to the tested bacteria with inhibition zones of  13.95 and 11.78 mm, respectively. The minimum inhibition concentration to the tested bacteria and Escherichia coli at 40% ammonium sulfate (0,1 M Tris-HCl of pH 8.3, 2 M NaCl, 0.01 M CaCl 2 saturation was 6000 µg/mL with inhibition zones of 9.78 and 8.73 mm, respectively. Based on the bioactivity test of the Hydroid protein fraction using the agar diffusion method, it can be concluded that the activity characteristic of the fraction is bacteriostatic.Key words: Bioactivity, hydroid, bioactive protein, antibacterial, inhibition zone.
Karakterisasi Sifat Fisikokimia Komposit Besi Oksida-Montmorilonit Hasil Interkalasi Silikat Lempung Montmorilonit Serly Jolanda Sekewael
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 1 No 1 - December 2008
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v1i1.2450

Abstract

The preparation of iron oxide-montmorillonite composite has been done by intercalation of iron oxide into the silicates interlayer of montmorillonite. Montmorillonite and iron oxide-montmorillonite composite were characterized to observe some physical and chemical properties. The observed properties were basal spacing d001, determined by X-Rays Difractometer (XRD); surface morphology, analyzed by SEM/EDAX; specific surface area, distribution of pore radius and total pore volume, analyzed by Gas Sorption Analyzer; functional groups, analyzed by FTIR Spectrophotometer; and iron content, determined by X-Ray Fluorescent Analyzer (XRF). The characterization of montmorillonite and iron oxide-montmorillonite composite showed that the basal spacing d001, increased from 14.11 Å in montmorillonite to 16.85 Å in iron oxide-montmorillonite. Surface morphology of montmorillonite showed the laminated structure, while that of iron oxide-montmorillonite indicated a delaminated structure with the external oxides layered. Specific surface area increased from 69.71 m2/g to 126. 49 m2/g; total pore volume also increased from 50.70 x10-3 mL/g to 107.89 x10-3 mL/g. Generally, spectrum adsorption of montmorillonite and iron oxide-montmorillonite composite not different so far, stretching vibration O-H with Fe3+ does not exist on iron oxide-montmorillonite composite. The iron content also increased from 4.57 %(w/w) to 23.61 %(w/w).Keywords : montmorillonite clay, intercalation, iron oxide-montmorillonit composite.

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