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Padjadjaran Journal of Dentistry

PJD
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Published by Universitas Padjadjaran

ISSN : 19790201 EISSN : 25496212 DOI : -

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Padjadjaran Journal of Dentistry or known as PJD, is an English-language scientific periodicals published by the Faculty of Dentistry Universitas Padjadjaran thrice a year on every March, July and November. The submission process of manuscript is open throughout the year. All submitted manuscripts will go through the double-blind peer review and editorial review before being granted with acceptance for publication. Designed as a medium of information and scientific knowledge, Padjadjaran Journal of Dentistry publishes numerous research articles, in the area of Oral Biology, Dental Material Science and Technology, Oral and Maxillofacial Surgery, Pedodontics, Dental Public Health and Community Dentistry, Conservative Dentistry, Periodontics, Prosthodontics, Orthodontics, Oral Medicine, Dental Radiology, as well as with their development through interdisciplinary and multidisciplinary approach. Initially published as Padjadjaran Journal of Dentistry (PJD) magazine ISSN 1979-0201(print) on 2007 in form of printed issues. In 2017, the magazine had its online published version under the same with ISSN 2549-6212 (online) in accordance with the policy of LIPI. Padjadjaran Journal of Dentistry has been using Open Journal System requiring all writers to register in advance before they are allowed to upload the manuscript they write online. Afterwards, the editors, peer reviewers, and writers can monitor the manuscript processing. Several other changes are informed in the Journal History.

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Search results for , issue " Vol 21, No 3 (2009): November" : 12 Documents clear

The correlation between serum neuron-specific enolase level in adult patients of oromaxillofacial fracture with mild head injury Ruslin, M.; Yusup, HY; Arifin, MZ; Noormartany, N.
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Oromaxilofacial fracture will have associated with head injury due to its adjacent location and structure. The oromaxillofacial structure is considered to be as force silencer for trauma so that it can protect the intracranial structures. Neuron-specific enolase (NSE) is a specific enzyme within neuron cells and can be detected rapidly in serum. The objective of the study was to investigate the increased serum NSE level in adult patients with oromaxillofacial fracture with mild head injury; and to investigate the correlation between serum NSE level and the location of oromaxillofacial fracture (upper, middle, lower regions, or the combinations) in adult patients with mild head injury. This study used the analytical observational method with a cross-sectional design, that conducted in Department of Neurosurgery, Department of Oral and Maxillofacial Surgery, and Laboratory of Clinical Pathology, Dr Hasan Sadikin Hospital, Bandung, from August to October 2008. The serum NSE level examination was undertaken by blood sample taking then examined with electrochemiluminescence immunoassay (ECLIA). The data were analyzed using Mann-Whitney test to seek the increased serum NSE level; using Kruskal-Wallis to seek the mean of increased serum NSE levels by location of oromaxillofacial fracture; and using Spearman ranked correlation test to seek the correlation between the increased serum NSE levels and location of oromaxillofacial fracture in adult patients with mild head injury. The study results indicated that there was an increased serum NSE level in adult patients with oromaxillofacial fracture with mild head injury by 13.12 ng/mL compared to adult healthy group by 7.72 ng/mL (p<0.001). By upper, middle, and lower regions of oromaxillofacial fracture, the serum NSE level of oromaxillofacial fracture at combined upper-middle-lower region was significantly the highest, i.e., 21.14 ng/mL (p=0.049). A positive correlation between the increased serum NSE level and location of oromaxillofacial fracture caused by mild head injury existed (rs=0.33, p=0.020). In conclusion, there was an increased serum NSE level in adult patients with oromaxillofacial fracture with mild head injury; and there was also a positive correlation between the increased serum NSE level and location of oromaxillofacial fracture due to mild head injury.

Optimalization of DNA isolation from oral epithelial mucous cell with smear method Nasroen, Saskia L.; Maskoen, Ani Melani; Nurwiadh, Agus
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Deoxyribonucleic acid (DNA) is a genetic material which is found in all living organisms. On the human cell or eukaryotes cell, the DNA is found in the nucleus cell and the mitochondria. The DNA arrangement on each cell in human body is the same, that is why, for the analysis meaning, DNA can be isolated from any cell in the body. The source of DNA to be analyzed usually coming from the blood sample by an injection method, such a way resulting in pain and bringing about constraint. Therefore, a study was carried out to look for an alternative of DNA isolation. The aim of this experimental study was to get an optimal DNA isolation method by using oral mucous smear method with a purpose to get a quick and easy DNA isolation. The investigation materials were in the form of samples which were taken from the oral epithelial mucous cells out of three different subjects. The epithelial cells were obtained by the oral mucous smear method which in a variation of two, four and six times of smear applications, respectively. The DNA was then isolated using buffer extraction method. The concentrations of DNA were measured by using ultraviolet spectrophotometer at 260 nm wavelength. The results of DNA isolation were analyzed by Polymerase Chain Reaction (PCR) technique. The optimal DNA isolation could be analyzed by PCR technique. The experimental results show that from three different subjects of study, DNA can be isolated optimally by oral mucous smear method with six times of smear applications.

Altered immunoexpression of e-cadherin and C-erbB2 in salivary gland adenoid cystic carcinoma: Correlation with the degree of histopathological malignancy Mariam, Marry Siti
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Adenoid Cystic Carcinoma (ACC) is a type of malignancy found in salivary gland, but its neoplasm prognosis is hard to predict. It grows slowly but spread continuously to its surrounding tissues and metastasizes hematogenously with a very high level of relapse. Histological, ACC of salivary gland is a combination of three types of growth phases (tubular, cribiform and solid), the solid type is > 30%, represented the worst prognosis. C-erbB2 proto-oncogene assumed as a prognosis symptom of salivary gland carcinoma, whilst the result of C-erbB2 expression in ACC salivary gland of was remained to be controversial. E-cadherin immunoexpression in ACC of breast gland used as the symptom of malignancy. Some experiments found that C- erbB2 oncogene product can be inducted the decrease of E-cadherin gene expression. Therefore, this experiment was conducted to find out the alteration of E-cadherin adhesion protein immunoexpression and C-erbB2 proto-oncogene in salivary gland ACC, in its correlation with the degree of its histopathological malignancy. The research design was a cross-sectional study at 51 block of paraffin which produced retrospectively by the patients with adenoid cystic carcinoma of salivary gland. All the samples of tumors were re-diagnosed to get the data of histopathological malignancy degree (as a modification of Szantos and Batsakis methods) with the smear of HE. Afterward, they got an immunohistochemistry, toward E-cadherin and C-erbB2. The result of the research showed that E-cadherin expression has negative correlation with the increase of histopathological malignancy degree. The low or non-existing of E-cadherin in salivary gland is related to high degree of histological malignancy (p< 0.01). Whilst the changed of E-erbB2 expression had significant correlation with the degree of histopatological malignancy (p < 0.03), but itâs correlation with the increase of histopathological malignancy was insignificant (p = 0.11). In summary, E-cadherin expression could be used as an indication of tumor to predict the prognosis malignancy of adenoid cystic adenoma of salivary gland. C-erbB2âs role in salivary gland ACC related to its biological acts.

Electro-gene therapy followed by intratumoral injection of pcDNA3.1-p27Kip1 wild type in human tongue base cancer cells SP-C3 xenograft Supriatno, S.; Sasmita, Inne Suherna
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Human tongue base cancers are characterized by a high degree of local invasion and metastasis to the regional lymph nodes and included a disease with difficult treatment. A novel method for high-efficiency and region-controlled in vivo gene transfer was developed by combining electro-gene therapy and plasmid (pcDNA). The aims of the study were to examine the efficiency of transfection of p27Kip1 gene by electro-gene therapy and to evaluate p27Kip1 gene therapy in Supriâs clone-3 (SP-C3) xenografts using pcDNA3.1-p27Kip1 wild-type (wt) and pcDNA3.1 empty vector (neo) with electro-gene therapy. To investigate gene transfer method, the enhanced green fluorescence protein (EGFP) gene was transfected into xenografts by electro-gene therapy. The efficiency of p27Kip1 gene transfection at protein level was confirmed by Western blotting. To estimate the reduction in tumour size in Wistar Balb/c mice after electro-gene therapy with p27Kip1 wt gene was examined by tumorigenesis assay. To evaluate the induction of apoptosis was carried out by colourimetric assay. The result, the growth of tumours was markedly suppressed by p27Kip1 wt gene transfection. Up-regulation of p27Kip1 protein was detected in pcDNA3.1-p27Kip1 wt. Apoptosis induction through the activity of caspase -3 and -9 was significantly increased in p27Kip1 wt-transfected tumours. These results suggest that it is possible to transfer p27Kip1 wt into tongue base cancer cell xenografts using electro-gene therapy. p27Kip1 wt had a high-potential to suppress the growth of tumours. Conclusion, electro-gene therapy followed by intratumoral injection of pcDNA3.1-p27Kip1 wt had a high-potential to suppress the growth of a human tongue base cancer cell xenograft.

Arterial stiffness in patients with localized and generalized severe chronic periodontitis: A preliminary study Arief, Erry Mochamad; Khee, Ho Ting; Rehman, Asia; Hassan, Akram; Khamis, Mohd. Fadhli
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

A relationship between periodontal disease and cardiovascular disease (CVD) has been reported in several studies. This association was explained based on the chronic systemic inflammatory response which may lead to arterial stiffness in chronic periodontitis (CP). To compare arterial stiffness as carotid-femoral pulse wave velocity (PWV) between patients with localized severe CP (LSCP) and generalized severe CP (GSCP). Five patients with LSCP and 12 patients with GSCP were recruited in a cross-sectional study. Subjects were excluded if they had systemic diseases other than CP. Diagnosis of LSCP and GSCP was made based on probing depth and radiographic bone loss. Carotid-femoral PWV was measured using the CompliorÂ® machine. Data were analyzed using SPSS. Carotid-femoral PWV was higher among patients with GSCP as compared to those with LSCP although the difference did not reach a significant level. Median (interquartile range) PWV was 7.5 (1.50) and 7.0 (1.03) m/sec respectively in patients with GSCP and LSCP (p = 0.224). Among patients with CP, aorta is stiffer in GSCP than in LSCP

The prevalence of Stevens Johnson Syndrome caused by antiretroviral in hospitalized patients at Dr. Hasan Sadikin General Hospital Bandung Maelani, Nurmilah; Sufiawati, Irna; Darmadji, Hartati Purbo
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Stevens-Johnson Syndrome is a mucocutaneous disease caused by allergic drug eruption. Antiretroviral (ARV) therapy for HIV/AIDS patient may cause allergic drug eruption such as Stevens-Johnson Syndrome.The aim of this research was to find out the prevalence of Stevens-Johnson Syndrome caused by ARV in hospitalize patient at Dr.Hasan Sadikin General Hospital Bandung from January to December 2008.It was a descriptive research by taking the secondary data from patientâs medical record. The result of this research showed that from 20 Stevens-Johnson Syndrome patients, 12 persons of them (60%) are men.Most of the patients were between the age of 20-29 (45%).Oral manifestation of Stevens-Johnson Syndrome seen in 100% patients.Prevalence of Stevens-Johnson Syndrome caused by ARV was 28.6% which seen in 8 HIV/AIDS patients.ARV combination consists of nevirapine, lamivudine, and zidovudine was the most (50.0%) ARV which suspected causing Stevens-Johnson Syndrome.The conclusion of this research showed that the prevalence of Stevens-Johnson Syndrome caused by ARV in hospitalize patient at Dr.Hasan Sadikin General Hospital Bandung 2008 was 28.6% seen in 8 HIV/AIDS patients.

The comparison of color stability between thermoplastic nylon resin and heat-cured acrylic resin after tea-soaking using spectrophotometer Lestari, Lisa Putri; Usri, Kosterman; Febrida, Renny
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Color stability is one of the denture base material requirement. Nowadays, nylon thermoplastic resin and heat-cured acrylic resin which is used as denture base materials can be changed in colour. One of a colour-changed factor is the tea drinking habit. The objective of this research was to compare the colour stability between nylon thermoplastic resin and heat-cured acrylic resin after soaked in tea. Materials used for the research were nylon thermoplastic resin from Valplast and heat-cured acrylic resin QC-20, with a sample size of 64x(10Â±0.03)x(2.5Â±0.03)mm. The sample would be assessed of colour stability after the denture base soaked in tea for 7 days. The colour measurement of each sample was done by spectrophotometer equipment (CIE lab system). Data gathered later on statistically processed with ANOVA testing. The result showed that the comparison ratio of colour stability between nylon thermoplastic resin and heat-cured acrylic resin after soaked in tea was 1.9886:1.1152. The conclusion was that colour stability in the nylon thermoplastic resin was lower than heat-cured acrylic resin after soaked in tea. This result was due to a polymer chain of nylon thermoplastic which was lower than heat-cured acrylic resin and its porosity which was higher, so tea easily soaked into the materials.

The effect of giving NaOCL 2.5% and H2O2% solution on the initial setting Mineral Trioxide Aggregate (MTA) Wardhani, Saraswati Dian; Usman, Munyati; Kamizar, K.
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Today, Mineral Trioxide Aggregate (MTA) is one of the most commonly-used materials to overcome problems in dental pulp as well as periodontal tissue. This substance received its permission from the American Food and Drug Administration (FDA) in 1998 and has been patented as a Portland type I.ASTM C150 cement-base material. However, MTA still has its flaws, such as: its granule form, requirement for moist condition, mass losing during hydration (high solubility), and particularly its time-consuming setting duration (45â-165â). Some researches attempt the usage of regular accelerator together with Portland cement, yet thereâs still no certainty of the best concentration that can be used to gain optimal result. This research conducted a time test of the initial setting of MTA that was mixed with the strong-oxidation antimicrobial solutions, they are NaOCl 2.5% dan H2O2 3%. The instrument used was the Gillmore needle. Data analysis used one-way ANOVA and Mann Whitney significance test (p<0,05). The result of the research showed that the mixing of NaOCl 2.5% with MTA the initial setting was Â± 11 minutes and the mixing of NaOCl 2.5% with MTA the initial setting was Â± 5 minute, it compared with the initial setting of original MTA which was the initial setting Â± 50 minutes.

The comparison of surface hardness between thermoplastic nylon resin and heat-cured acrylic resin Utami, Marina; Febrida, Renny; Djustiana, Nina
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

The surface hardness level of a denture base material is related to the resistance to abrasion, scratch, polishing and water sorption. Nowadays, thermoplastic nylon resins and heat-cured acrylic resins are used as denture base materials. Denture base must be immersed in water to maintain its humidity. The objective of this research was to compare the surface hardness level between thermoplastic nylon resins and heat-cured acrylic resins. Materials used for the research were thermoplastic nylon resins from Valplast and heat-cured acrylic resins QC-20, with a sample size of 64x(10Â±0.03)x(2.5Â±0.03) mm. Surface hardness is measured with Vickers Microhardness Tester and the data were analyzed by ANOVA method. The results showed that comparison ratio of surface hardness level between thermoplastic nylon resins and heat-cured acrylic resins before immersion is 3.2:7.3 VHN respectively, while the comparison value of both resins after immersion is 2.8:6.6 VHN. In conclusion, the surface hardness level of thermoplastic nylon resins is lower compared to heat-cured acrylic resins. This result is due to thermoplastic nylon resinâs higher porosity, its different polymer chains, and the plasticizers effect after immersion in water.

A retrospective clinicopathologic study of lichen planus and lichenoid lesions in the oral cavity Primasari, Ameta
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Lichen planus is a common oral disorder which may represent the manifestation of varies clinical presentation and microscopic findings. In this retrospective study of 86, cases of oral lichen planus (OLP) were compared with 73 cases of oral lichenoid lesions (OLL). Various clinicohistopathological features were studied. The object of this study was to compare clinical and microscopic findings in order to present evidence that supports the position of true lichen planus. Biopsy specimens were obtained from every patient. The biopsy specimens were fixed in 10 percent formalin, embedded in paraffin, sectioned at 4 microns and stained by means of routine hemotoxylin and eosin procedures. The histologic specimens were examined and evaluated without knowledge of clinical findings. The mean age at presentation of patients with OLP was 42.5 years as compared to 47.0 years for OLL. There was no significant difference between the ethnic groups, site of lesions and the distribution of the clinical presentation. Compare to histopathologic findings, there were only 55% clinical and histological agreement in this study, this because of using strict criteria for oral lichen planus there could be an over diagnosis lesions. This study showed that there were no reliable clinical and histopathological features which could differentiate OLL from OLP. The features in the OLL group were non specific. The patientâs medical history, oral habits or psychological status appeared to be able to alter the classical clinicopathological findings.

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