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Altered immunoexpression of e-cadherin and C-erbB2 in salivary gland adenoid cystic carcinoma: Correlation with the degree of histopathological malignancy Mariam, Marry Siti
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Adenoid Cystic Carcinoma (ACC) is a type of malignancy found in salivary gland, but its neoplasm prognosis is hard to predict. It grows slowly but spread continuously to its surrounding tissues and metastasizes hematogenously with a very high level of relapse. Histological, ACC of salivary gland is a combination of three types of growth phases (tubular, cribiform and solid), the solid type is > 30%, represented the worst prognosis. C-erbB2 proto-oncogene assumed as a prognosis symptom of salivary gland carcinoma, whilst the result of C-erbB2 expression in ACC salivary gland of was remained to be controversial. E-cadherin immunoexpression in ACC of breast gland used as the symptom of malignancy. Some experiments found that C- erbB2 oncogene product can be inducted the decrease of E-cadherin gene expression. Therefore, this experiment was conducted to find out the alteration of E-cadherin adhesion protein immunoexpression and C-erbB2 proto-oncogene in salivary gland ACC, in its correlation with the degree of its histopathological malignancy. The research design was a cross-sectional study at 51 block of paraffin which produced retrospectively by the patients with adenoid cystic carcinoma of salivary gland. All the samples of tumors were re-diagnosed to get the data of histopathological malignancy degree (as a modification of Szantos and Batsakis methods) with the smear of HE. Afterward, they got an immunohistochemistry, toward E-cadherin and C-erbB2. The result of the research showed that E-cadherin expression has negative correlation with the increase of histopathological malignancy degree. The low or non-existing of E-cadherin in salivary gland is related to high degree of histological malignancy (p< 0.01). Whilst the changed of E-erbB2 expression had significant correlation with the degree of histopatological malignancy (p < 0.03), but itâs correlation with the increase of histopathological malignancy was insignificant (p = 0.11). In summary, E-cadherin expression could be used as an indication of tumor to predict the prognosis malignancy of adenoid cystic adenoma of salivary gland. C-erbB2âs role in salivary gland ACC related to its biological acts.

The performance of 1% solution of baking soda as the mouthwashing for elderly xerostomia patients on the salivary secretion Ariyanti, Ririn; Tjahajawati, Sri; Mariam, Marry Siti
Padjadjaran Journal of Dentistry Vol 30, No 1 (2018): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Introduction: The ageing process in older adults could cause a decreasing salivary secretion. Indicators of salivary secretion could be measured by observing an increasing salivary volume and pH, and a decreasing salivary viscosity. This study was aimed to observe the performance of 1% solution of baking soda on the salivary secretion of elderly xerostomia patients. Methods: The study used the quasi-experimental method with purposive sampling technique. The subjects were 24 older adults who were administered with 1% solution of baking soda. Results: The results showed that the averages volume, pH, and viscosity of the saliva before rinsing with 1% solution of baking soda were lower than after rinsing with the solution (p < 0.005). The average values of volume and pH of saliva before rinsing of 1% solution of baking soda were 0.15ml/min and 5.4, respectively, whilst after rinsing with the solution were 0.14ml/min and 7.42, respectively. The difference in saliva viscosity before and after rinsing with the solution showed that there were changes in the viscosity from frothy to watery. Conclusion: 1% solution of baking soda solution was able to increase the salivary secretion of elderly xerostomia patients, observed from an increasing salivary volume and pH, and a decreasing salivary viscosity after rinsing with 1% solution of baking soda.

Bacterial inhibition test of methanolic extracts of strawberry (Fragraia x ananassa Duchesne), lime (Citrus aurantifolia), and radish (Raphanus sativus L.), towards Streptococcus Sanguis ATCC 10556 Anggraini, Fitri; Satari, Mieke Hemiawati; Mariam, Marry Siti
Padjadjaran Journal of Dentistry Vol 30, No 2 (2018): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Introduction: Caries are initiated by the plaque formation on the tooth surface, due to the interaction between food debris and bacteria in the mouth. The pioneer bacterium of plaque formation is Streptococcus sanguis. TheÂ Strawberry fruit, lime fruit, and radish root are part of the plant that contains antibacterial substances. Flavonoid, tannin, saponin, alkaloid, polyphenol, terpenoid and quinon as antibacterial substances. This study aimed to evaluate strawberry fruit, lime fruit, and radish root methanol extract can inhibit Streptococcus sanguis ATCC 10556. Therefore, strawberry, lime, and radish can be made into mouthwash, bubble gum, or toothpaste to prevent plaque formation. Methods: This study used a Kirby-Bauer diffusion test as the inhibition test. Results: The result of this study that the biggest inhibition zone was lime methanol extract 20,000 ppm, and the smallest one was radish methanol extract 5,000 ppm. Â Conclusion: Strawberry fruit, lime fruit peel, and radish root methanol extract and their combination had the effect of inhibiting bacterial growth synergistically against Streptococcus sanguis ATCC 10556.

Hubungan Imunoekspresi E-cadherin dan C-erbB2 dengan Derajat Keganasan Histopatologik Karsinoma Kistik Adenoid Kelenjar Liur Mariam, Marry Siti; Nasserie, Wazilah
Majalah Kedokteran Bandung Vol 44, No 2 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1624.831 KB)

Karsinoma kistik adenoid (KKA) merupakan keganasan kelenjar liur terbanyak, prognosisnya sukar diprediksi dengan tingkat kekambuhan yang tinggi. Berdasarkan beberapa penelitian, prognosis KKA kelenjar liur yang berhubungan dengan tingkat ketahanan hidup sangat terkait dengan derajat keganasan histopatologik berdasarkan tipe pola pertumbuhannya. Penelitian ini dilaksanakan di Departemen Patologi Anatomi Fakultas Kedokteran Universitas Padjadjaran Bandung tahun 2009. Tujuan penelitian untuk mengetahui perubahan imunoekspresi protein adhesi E-cadherin (molekul adhesi antar sel epitel) dan proto-onkogen C-erbB2 (keluarga C-erbB/reseptor faktor pertumbuhan epidermal) pada KKA kelenjar liur dikorelasikan dengan derajat keganasan histopatologik. Desain penelitian studi potong lintang tanpa acak secara retrospektif pada 51 blok parafin penderita karsinoma kistik adenoid kelenjar liur. Sampel didiagnosis ulang, serta ditentukan derajat keganasan histopatologiknya (menurut modifikasi Szantos dan Batsakis) dilanjutkan dengan pemeriksaan imunohistokimia terhadap E-cadherin dan C-erbB2. Hasil menunjukkan korelasi sangat bermakna (p<0,01) perubahan imunoekspresi E-cadherin yang negatif (82%) dengan derajat keganasan histopatologik 1, 2, dan 3 (4%, 33%, dan 46%). Perubahan imunoekspresi C-erbB2 tidak berkorelasi dengan meningkatnya derajat keganasan (p=0,11). Terjadi perubahan peningkatan imunoekspresi dari derajat 1 (5%) ke derajat 2 (11%), namun kembali menurun pada derajat 3 (8%). Simpulan, imunoekspresi E-cadherin dapat digunakan sebagai petanda tumor untuk memprediksi prognosis keganasan KKA kelenjar liur. Perubahan imunoekspresi C-erbB2 pada KKA kelenjar liur menunjukkan perilaku biologinya danÂ peran utama C-erbB2 pada KKA kelenjar liur terutama pada fase inisiasi dan promosi karsinogenesis. [MKB.2012;44(2):70â€“6].Correlation of E-cadherin and C-erbB2 Immunoexpression with theÂ Degree of Histopathological Malignancy on Salivary Gland Adenoid Cystic CarcinomaAdenoid cystic carcinoma (ACC) is the most common salivary gland malignancies, with high rate of local recurrence and unpredictable prognosis. Based on previous research, prognosis of ACC in salivary gland which is correlated with survival rates, is related with histopathological malignancy degree based on its growth pattern type. This study was conducted in Pathology Anatomy Department of Medical Faculty, Padjadjaran University Bandung in 2009. The aim of this study was to analyze the alteration of immunoexpression of E-cadherin (adhesion molecule of epithelial cells) and C-erbB2 proto-oncogen (the family of C-erbB/epidermal growth factor receptor) in salivary gland. Adenoid cystic carcinoma correlated with cross-sectional non-random study on 51 paraffin blocks, from patients with salivary gland ACC retrospectively. The repeated histopatologic examination was to diagnose ACC and to get data of the histopathological malignancy degree (according to Szantos and Batsakis modification), and it was continued with immunohistochemistry examination of E-cadherin and C-erbB2. The alteration of negative immunoexpression of E-cadherin (82%) had correlation significantly (p<0.001) with the histological malignancy degrees 1, 2, and 3 (4%, 33% and 46%). The C-erbB2 immunoexpression change had no correlation with the increasing histopatologic malignancy degree (p=0.11). The alteration of C-erbB2 immunoexpression, increased from first (5%) to second degree (11%) but decreased on the third degree (8%). In conclusions, the immunoexpression of E-cadherin can be used as tumor marker to predict malignancy prognosis of salivary gland ACC. The expression changes of C-erbB2 in ACC indicate its biological behavior and the main role of C-erbB2 on salivary gland ACC is in the initiation and promotion phase of carcinogenesis. [MKB. 2012;44(2):70â€“6].Â DOI:Â Â http://dx.doi.org/10.15395/mkb.v44n2.126

Variasi normal lidah manusia pada subras Deutro MelayuNormal variation of human tongue on Deutro Malay subrace Denta Asnatasia Nurmadhini; Winny Yohana; Marry Siti Mariam
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 31, No 1 (2019): April 2019
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Pendahuluan: Lidah merupakan organ yang terdiri dari otot rangka dan dilapisi oleh membran mukosa. Organ ini merupakan salah satu indikator yang baik untuk diagnosis secara klinis dan karakteristiknya dapat dipengaruhi oleh ras. Tujuan penelitian adalah untuk mengetahui gambaran variasi normal lidah manusia pada subras Deutro Melayu sebagai suatu karakteristik lidah. Metode: Penelitian deskriptif dilakukan pada 96 mahasiswa Fakultas Kedokteran Gigi Universitas Padjadjaran angkatan 2014 yang terdiri dari 87 wanita dan 9 laki-laki. Sampel diambil dengan teknik purposive sampling. Penelitian dilakukan dengan mengamati lidah secara visual dalam keadaan protrusif dan tidak tegang. Lidah diamati berdasarkan bentuk dan tekstur permukaan kemudian difoto untuk dokumentasi. Analisis data dilakukan dengan distribusi frekuensi relatif. Hasil: Persentase bentuk lidah yang paling banyak ditemukan adalah persegi, membulat, persegi panjang, dan segitiga tajam, masing-masing sebanyak 46,87%; 44,79%; 5,20%; 3,12%; dan tidak ditemukan bentuk segitiga tumpul. Tekstur permukaan yang paling banyak muncul adalah tanpa fisura, diikuti oleh fisura vertikal sentral, vertikal lateral, sentral, dan horizontal sebanyak masing-masing 54,17%; 18,75%; 14,58%; 8,33%; dan 4,17%. Simpulan: Variasi normal bentuk lidah manusia yang paling sering ditemukan pada subras deutromelayu adalah persegi, sedangkan tekstur permukaan lidah paling banyak tanpa fisura.Kata kunci: Variasi normal, lidah, subras Deutro Melayu ABSTRACTIntroduction: Tongue is an organ consisted of skeletal muscle and covered by mucous membranes. This organ is a good indicator of clinical diagnosis, and its characteristics can be influenced by race. This study was aimed to describe the normal variation of the human tongue on Deutro Malay subrace as the tongue characteristics. Methods: A descriptive study was conducted on 96 students of Faculty of Dentistry Universitas Padjadjaran batch 2014, consisted of 87 women and 9 men. Samples were taken by purposive sampling technique. The research was carried out by observing the tongue visually in a protrusive and relax state. The tongue was observed based on the shape and surface texture then photographed for documentation. Data analysis was performed by relative frequency distribution. Results: The most found tongue shape was square, followed by rounded, rectangular, and sharp triangles, which were 46.87%; 44.79%; 5.20%; and 3.12% respectively, with no blunt triangle was found. The surface texture most appeared was without fissure, followed by a central vertical, vertical, central, and horizontal fissure, which were 54.17%; 18.75%; 14.58%; 8.33%; and 4.17% consecutively. Conclusion: The normal variation in the tongue shape of Deutro Malay subrace mostly found is square and the most surface texture is without fissure.Keywords: Normal variation, tongue, Deutro Malay subrace

The linear polymerisation shrinkage effect of chemical activated composite resin using different bonding agents on the dimensions of the tooth cavity Marry Siti Mariam; Arief Cahyanto
Padjadjaran Journal of Dentistry Vol 32, No 1 (2020): March 2020
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol32no1.25915

Introduction: This study was aimed to determine the effect of the linear polymerisation shrinkage of composite resin using different bonding agents on the dimension of the tooth cavity. Methods: Fifty-four extracted human premolar teeth, both maxillary and mandibular were used and divided into three groups (A, B, and C) into eighteen specimens. The conventional cavity was prepared, and all cavities were bevelled. In Group A, only the bevel was etched, and the enamel bond was applied to it. Group B was treated with the same procedure as group A, but the dentin bond was used instead of enamel bond. Group C, the whole cavity was etched, dentin bond was applied on the bevel and the cavity walls. The reference points were placed in the vestibular and lingual palatal region as occlusal as possible on the enamel. The Silar® composite resin (microfilled, chemically activated), etching agent (gel etch), enamel and dentin bond system were employed for the preparation and filing of the cavities. The cavity was filled with composite, and the specimens were immersed in 0.02% chlorhexidine gluconate solution at the room temperature. The distance between both reference points was measured before and after filling procedures. Results: Group A showed the least shrinkage with a mean of 0.14%, group B showed a larger shrinkage with the average of 0.24% and the largest shrinkage was found in group C with the average of 0.26%. The difference between groups B and C was not statistically significant given at the point of 0.05 significance level, and the difference between groups A, B, and C was significant at the 0.01 significance level. From the mathematical modelling using the stress-strain equation, the results showed that the tensile stress in restrained of Silar® composite was higher than the enamel (19.80 MPa > 10.34 MPa) thus lead to cracks in the enamel. Conclusion: The acid etching on dentin is not improving the retention and marginal adaption of restorative resin. The use of enamel bond system gives a better effect on the adhesion strength compared to the dentin bond system.

Effect of cassava (Manihot esculenta cranzt), rice (Oryza sativa L.), and potato (Solanum tuberosum) water extract to decrease pH phase fermentation of Streptococcus mutans ATCC 25175 Reza Afri Imelda; Marry Siti Mariam; Mieke Hemiawati Satari
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Dental caries is the localised damage of the hard tooth tissue caused by acid production results of carbohydrate fermentation by acid-producing bacteria. Streptococcus mutans are one of the aetiological bacteria which fermented carbohydrate causes a decrease in the oral pH. Carbohydrate is generally consumed, included cassava, rice, and potato. The purpose of this study was to determine the mechanism of the water extract of rice, cassava, and potato in decreasing the pH of Streptococcus mutans culture to know their potential in causing caries and also determined decreasing differences between three water extracts. Methods: The experimental method was used in cultured Streptococcus mutans ATCC 25175 and tested against the treated rice, cassava, and potato water extract. Cultured bacteria were incubated for 48 hours in facultative anaerobes then measured the pH with a digital pH meter. The measurement result was statistically tested with the paired t-test and ANOVA. Results: The paired t-test (α = 0.05) showed that the p-value of rice, cassava, and potato water extract were 0.001, 0.001, and 0.018 respectively. ANOVA test with an α value of 0.05 showed the p-value of 0.000. Conclusion: There was a decrease in pH of cultured Streptococcus mutans after administration of the water extract of cassava, rice, and potato, and there was a difference between the pH decrease of the Streptococcus mutans between administration of water extract of rice, cassava, and potato. Keywords: Streptococcus mutans, Manihot esculentacrantz, Oryza sativa L., Solanum tuberosum, pH phase fermentation

Bacterial inhibition test of methanolic extracts of strawberry (Fragraia x ananassa Duchesne), lime (Citrus aurantifolia), and radish (Raphanus sativus L.), towards Streptococcus Sanguis ATCC 10556 Fitri Anggraini; Mieke Hemiawati Satari; Marry Siti Mariam
Padjadjaran Journal of Dentistry Vol 30, No 2 (2018): July 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Caries are initiated by the plaque formation on the tooth surface, due to the interaction between food debris and bacteria in the mouth. The pioneer bacterium of plaque formation is Streptococcus sanguis. The Strawberry fruit, lime fruit, and radish root are part of the plant that contains antibacterial substances. Flavonoid, tannin, saponin, alkaloid, polyphenol, terpenoid and quinon as antibacterial substances. This study aimed to evaluate strawberry fruit, lime fruit, and radish root methanol extract can inhibit Streptococcus sanguis ATCC 10556. Therefore, strawberry, lime, and radish can be made into mouthwash, bubble gum, or toothpaste to prevent plaque formation. Methods: This study used a Kirby-Bauer diffusion test as the inhibition test. Results: The result of this study that the biggest inhibition zone was lime methanol extract 20,000 ppm, and the smallest one was radish methanol extract 5,000 ppm. Conclusion: Strawberry fruit, lime fruit peel, and radish root methanol extract and their combination had the effect of inhibiting bacterial growth synergistically against Streptococcus sanguis ATCC 10556.

The performance of 1% solution of baking soda as the mouthwashing for elderly xerostomia patients on the salivary secretion Ririn Ariyanti; Sri Tjahajawati; Marry Siti Mariam
Padjadjaran Journal of Dentistry Vol 30, No 1 (2018): March 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: The ageing process in older adults could cause a decreasing salivary secretion. Indicators of salivary secretion could be measured by observing an increasing salivary volume and pH, and a decreasing salivary viscosity. This study was aimed to observe the performance of 1% solution of baking soda on the salivary secretion of elderly xerostomia patients. Methods: The study used the quasi-experimental method with purposive sampling technique. The subjects were 24 older adults who were administered with 1% solution of baking soda. Results: The results showed that the averages volume, pH, and viscosity of the saliva before rinsing with 1% solution of baking soda were lower than after rinsing with the solution (p < 0.005). The average values of volume and pH of saliva before rinsing of 1% solution of baking soda were 0.15ml/min and 5.4, respectively, whilst after rinsing with the solution were 0.14ml/min and 7.42, respectively. The difference in saliva viscosity before and after rinsing with the solution showed that there were changes in the viscosity from frothy to watery. Conclusion: 1% solution of baking soda solution was able to increase the salivary secretion of elderly xerostomia patients, observed from an increasing salivary volume and pH, and a decreasing salivary viscosity after rinsing with 1% solution of baking soda.

Oral hygiene status based on blood types of dentistry students Arini Puspitasari Deradjat; Marry Siti Mariam; Gilang Yubiliana
Padjadjaran Journal of Dentistry Vol 25, No 2 (2013): July 2013
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol25no2.26787

Introduction: Oral hygiene status can be measured by OHI (Oral Hygiene Index) the which is influenced by four factors: environmental, behavioral, hereditary and health services. Plaque and calculus Become an indicator of OHI, roomates poor OHI is one of the main causes of periodontal disease. Blood type as hereditary factors may be a risk factor for periodontal disease. More over, a dental health education will Affect a person’s behavior in dental and oral hygiene. The purpose of this research is to get an overview of the oral hygiene status of dental students in Padjadjaran University (Students in Regular Class 2008) based on blood types as a risk factor for periodontal disease, Methods:This study was a descriptive study with survey technique. Samples were taken with a simple purposive sampling method of 76 respondents, Results:The results Showed that the blood type O shows the best OHI-S with distribution frequency is 94.29% and the worst is blood type B with the distribution frequency is 6.25%, Conclusion: blood type O shows the best OHI-S and blood type B with the worst oral hygiene status is assume in having the biggest risk factor for periodontal disease compare to other blood types.

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