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All Journal JBIO: jurnal biosains (the journal of biosciences) Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Saintia Biologi JURNAL PENELITIAN SAINTIKA JURNAL PENGABDIAN KEPADA MASYARAKAT Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Chemistry Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Pembelajaran dan Biologi Nukleus KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Isnaini Nurwahyuni
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Other

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POTENSI BAKTERI KITINOLITIK DALAM PENGENDALIAN Aspergillus niger PENYEBAB PENYAKIT BUSUK PANGKAL AKAR PADA TANAMAN KACANG TANAH Arifda Ayu; Dwi Suryanto; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Abstract

A study of chitinolytic bacteria ability to control Aspergillus niger, a causal agent of basal root rot of peanut seedlings was conducted in Pest and Disease Laboratory, Medan Johor and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan.  The purpose of study is to evaluate the ability of Bacillus sp. BK13, Enterobacter sp. BK15, Bacillus sp. BK17, Enterobacter cloacae LK08, Bacillus sp. KR05, and Enterobacter sp. PB17 to inhibit the growth of A. niger on the peanut seedling.  Antagonistic test showed that the most effective bacteria in inhibiting the growth of A. niger was BK15 isolate with inhibition zone of 2,88 cm and BK13 isolate with inhibition zone of 2,69 cm, whereas the least effective bacteria was BK17, with inhibition zone of 2,30 cm. Chitinolytic bacterial isolates used to cover peanut seed through soaking enabled to reduce seed basal root rot. BK15 isolate showed potential to reduce the basal root rot by 58,82% while the lowest inhibition was BK13 by 47,06%.   Keywords: Aspergillus niger, Bacillus sp., chitinolytic bacteria, Enterobacter sp., peanut
ISOLASI DAN UJI POTENSI ISOLAT BAKTERI PELARUT FOSFAT DAN BAKTERI PENGHASIL HORMON IAA (Indole Acetic Acid) TERHADAP PERTUMBUHAN KEDELAI (Glycine max L.) PADA TANAH KUNING Dessy Merry Silitonga; Nunuk Priyani; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

Biofertilizer is a safe alternative fertilizer instead of various chemical fertilizers for increasing plant productivity which can minimize the ecological damage. There are plant symbiotic-soil bacteria which can solubilize phosphate or produce IAA. The objectives of this research are to determine the best soil bacteria in producing IAA or solubilizing phosphate and to know their roles in promoting plant growth in unfertile soil. As many as 8 isolates of phosphate solubilizing bacteria and 5 isolates of IAA producing bacteria were found. Among those isolates, there were 2 potential isolates (P2 & P4) that were able to solubilize phosphate and 1 potential isolate (I3) that was able to produce IAA. Isolate P4 produced holozone as wide as 1.45 cm and isolate P2 was 1.3 cm. Meanwhile, isolate I3 produced the highest concentration of IAA compared to the other isolates, that was 33.3 ppm. These isolates were applicated further on unfertile soil (yellow soil) as a medium for soybean growth. Soybeans have been grown for 10 weeks to observe the effect of those potential isolates on plant growth and productivity. The result showed that application of  isolate I3 was able to promote plant growth and increase plant productivity better than the other isolates as well as controls (fertile and unfertile soil). It promoted the soybean growth such as plant height was 58,8 cm; plant fresh weight was 3.5 g; plant dry weight was 1.55; pod fresh weight was 1.23; pod dry weight was 1.03; the amount of the pod was 3 and the number of the seed was 7.   Keywords : Phosphate solubilizing bacteria, IAA producing bacteria, biofertilizer, soybean.
ANALISIS KARIOTIPE DAN PERTUMBUHAN KACANG KEDELAI (Glycine max L.) SETELAH DIINDUKSI SINAR ULTRAVIOLET Rani Ernita Limbong; Elimasni D; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 3 (2013)
Publisher : Saintia Biologi

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Abstract

“Karyotype and Growth Analysis of Soybean (Glycine max L.) after Exposed by Ultraviolet light” has been done at Genetic and Molecular Biology Laboratory at Natural Scienes Faculty and the greenhouse at Agriculture Faculty Sumatera Utara University Medan. The objective was to investigate design karyotype and vegetative growth of soybean that induced by UV light. The experimental design was used complete factorial randomized with 2 factors. The first are Ultraviolet (UV) light 3 levels : 10, 20 and 30 Watt and the second is the time of UV exposure with 3 levels : 5, 10, and 15 minutes. Each treatment replicates 2 times and plant that not exposed by UV used as control. The karyotype was observed by squash method. The results showed that the intensity and duration of UV light have no effect on the number of chromosomes but the light has effect on the number of chromosome type. Statistical analysis showed that the increase in UV energy up to 30 Watt and time exposure of 15 minutes, caused the increase in height, number of leaves, number of flowers, the fresh and dry weight of the plant, and chlorophyll concentration compared to untreated plants. Keywords: karyotype, mutation, soybean, ultraviolet.
Perubahan Histologi dan Fisiologi Latisifer pada Tanaman Karet (Hevea brasiliensis Muell Arg.) yang diberi Asam Jasmonat dan Asam Naftalen Asetat Eksogen Radite Tistama; Vahnoni Lubis; Isnaini Nurwahyuni
Buletin Anatomi dan Fisiologi Volume 2, Nomor 1, Tahun 2017
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.2.1.2017.1-10

Abstract

Produktivitas tanaman karet tergantung pada faktor dominan seperti jumlah latisifer dan aktivitas metabolism lateks. Perkembangan histologi dan fisiologi latisifer tanaman karet dipengaruhi oleh jenis klon dan interaksi antara zat pengatur tumbuh eksogen dengan klon. Perubahan histologi dan fisiologi latisifer Hevea brasiliensis diteliti dengan aplikasi asam jasmonat, asam naftalen asetat dan kombinasi keduanya pada permukaan tunas lateral muda. Hasil penelitian ini menunjukkan bahwa zat pengatur tumbuh menginduksi jumlah pembuluh kateks tetapi mengurangi perkembangan tebal kulit dan ukuran sel latisifer. Kombinasi JA dengan NAA paling efektif menginduksi jumlah pembuluh lateks pada klon metabolisme tinggi PB 260. Zat pengatur tumbuh memacu perubahan aktivitas fisiologi pada klon metabolisme rendah IRR 42. Analisis histologi kulit dan fisiologi lateks pada tunas muda tanaman karet dapat digunakan sebagai pendeteksi potensi produktivitas dan sifat metabolisme tanaman karet. Kata kunci: Latisifer Hevea; asam jasmona; asam naftalen asetat; metabolisme lateks
The Development of Reproducible and Selective Uric Acid Biosensor by Using Electrodeposited Polytyramine as Matrix Polymer Manihar Situmorang; Isnaini Nurwahyuni
Indonesian Journal of Chemistry Vol 17, No 3 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.143 KB) | DOI: 10.22146/ijc.25818

Abstract

A versatile method for the construction of reproducible and high selective uric acid biosensor is explained. Electrodeposited polytyramine is used as biosensor matrixes due to its compatibility to immobilize enzyme uric oxidase in the membrane electrode. The precise control over the charge passed during deposition of polytyramine allows concomitant control over the thickness of the deposited enzyme layers onto the surface of the electrode. The uric acid biosensor showed a sensitive response to uric acid with a linear calibration curve lies in the concentration range of 0.1–2.5 mM, slope 0.066 µA mM-1, and the limit detection was 0.01 mM uric acid (S/N = 3). The biosensor shown excellent reproducibility, the variation between response curves for uric acid lies between RSD 1% at low concentrations and up to RSD 6% at saturation concentration. Uric acid biosensor is free from normal interference. The biosensor showed good stability and to be applicable to determine uric acid in real samples. Analysis of uric acid in the reference standard serum samples by the biosensor method are all agreed with the real value from supplier. Standard samples were also analyzed independently by two methods: the present biosensor method and the standard UV-Vis spectrophotometry method, gave a correlation coefficient of 0.994. This result confirms that the biosensor method meets the rigid demands expected for uric acid in real samples.
Quality of Effective Microorganisms-based Liquid Fertilizer from Fermented Papaya Fruits (Carica papaya L.) Wilander Simarmata; Yurnaliza Yurnaliza; Isnaini Nurwahyuni
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 2 (2022): Jurnal Pembelajaran Dan Biologi Nukleus Juli 2022
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i2.2733

Abstract

Papaya (Carica papaya L.) is one of the most popular fruits in the community and is generally only used as an ingredient for consumption in a fresh state. Papaya fruit production tends to be dynamic where sometimes the abundant amount of fruit may not necessarily be marketed directly. This causes the stock of papaya fruit during storage to rot and cannot be reused. This study aims to explore the potential of ripe papaya fruit to be processed as a substrate for the manufacture of liquid fertilizer using local microorganisms or effective microorganisms (EM). The quality of EM-based liquid fertilizer was determined based on the bacterial population density (CFU/mL) using the standard plate count (SPC) method on different growth media, then the levels of IAA and NPK in the applied fermented papaya fruit. The results showed the occurrence of nitrogen fixing bacteria, phosphate solubilizing bacteria and chitinolytic bacteria besides the cosmopolite bacteria. The optimum level of IAA in the fermented papaya fruit was EM + molasses (100%) while the NPK profile was still higher in the EM treatment without molasses.
RESPONS PERTUMBUHAN TANAMAN KEDELAI HITAM (Glycine soja L) MELALUI POC AIR TAHU DAN AIR KELAPA Rahma Dina; Isnaini Nurwahyuni; Elimasni Elimasni
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.12439

Abstract

The Study aims to see used of POC from tofu and coconut water waste has a significant effect in the growth of black soybean (Glycin soja L.) on the parameters of stem height, number of branches and leaves, and the number of stem diameters of black soybean plants. The research was conducted from September to December 2021 in kayu besar street, Batangkuis district. The study used RAK on 2 factorials, namely the treatment of giving POC from tofu and coconut water and concentrations at the levels of 0%, 15%, 20%, and 25%.The data analysis technique used the one way ANOVA test at a significant level of 5%and the DMRT test. The results showed that the use of POC from coconut air and air had a significantly different effect at a concentration of 25% better than other concentrations, namely 20%, 15%, and 0%. This study gave a better effect on the use of POC than those without fertilizers due to the addition of nutrients provided to increase the growth of black soybean plants.Key words: Black Soybean, POC, Water Tofu, Water Coconut. 
RESPONS PERTUMBUHAN TANAMAN KEDELAI HITAM (Glycine soja L) MELALUI POC AIR TAHU DAN AIR KELAPA Rahma Dina; Isnaini Nurwahyuni; Elimasni Elimasni
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.12439

Abstract

The Study aims to see used of POC from tofu and coconut water waste has a significant effect in the growth of black soybean (Glycin soja L.) on the parameters of stem height, number of branches and leaves, and the number of stem diameters of black soybean plants. The research was conducted from September to December 2021 in kayu besar street, Batangkuis district. The study used RAK on 2 factorials, namely the treatment of giving POC from tofu and coconut water and concentrations at the levels of 0%, 15%, 20%, and 25%.The data analysis technique used the one way ANOVA test at a significant level of 5%and the DMRT test. The results showed that the use of POC from coconut air and air had a significantly different effect at a concentration of 25% better than other concentrations, namely 20%, 15%, and 0%. This study gave a better effect on the use of POC than those without fertilizers due to the addition of nutrients provided to increase the growth of black soybean plants.Key words: Black Soybean, POC, Water Tofu, Water Coconut. 
Mitigation and Innovation in Making Natural Handwash Made from Coffee Grounds in Dusun X Batangkuis Deli Serdang Schools Towards Face-to-Face Schools Elimasni; Isnaini Nurwahyuni; Deny Supriharti; Rizky Yudha Pratama
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 2 (2023): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/abdimastalenta.v8i2.13932

Abstract

The government's desire to reopen and implement offline education has become a hot topic of discussion lately. The risk of spreading covid 19 to children is the concern of many people. For this reason, mitigation and prevention efforts need to be carried out to reduce the potential for the spread of covid 19. One effort that can be done is to make hand washing a good habit. The activity was carried out by providing training in making liquid hand soap for the community, especially teachers and school students in Dusun X to socialize how to prevent the spread of Covid-19 in schools. Activities are carried out through 5 (five) stages which include (1) Survey (2) Soap Making Test (3) Socialization; (4) Soap-making skills training; (5) Monitoring and Evaluation. It is hoped that the manufacture of natural handwash can be used for schools and school children in Dusun X Batangkuis Deliserdang to prevent covid 19 from going to face-to-face school in 2021.
Co-Authors . Rosmayati ., Rosmayati Andini - Hanif Arifda Ayu Deny Supriharti Dessy Merry Silitonga DWI SURYANTO Elimasni Elimasni Fathurrahman Fathurrahman Fathurrahman Fathurrahman Fauziah Harahap Fauziah Harahap Hasianna Nopina Situmorang Justin A. Napitupulu manihar Situmorang, manihar Mayang Sari Yeanny Mizawarti Mizawarti Napitupulu, Justin A Nunuk Priyani Radite Tistama Rahma Dina Rani Ernita Limbong Rizky Yudha Pratama Rizky Yudha Pratama Rizky Yudha Pratama Rizky Yudha Pratama Silitonga, P Maulim Sinaga, Rianto Sinaga, Riyanto Siregar, Linda Sari Steven Taniwan Vahnoni Lubis Wilander Simarmata Yurnaliza, Yurnaliza