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All Journal Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) Preventif Journal Indonesian Journal of Pharmaceutical and Clinical Research Pharmaceutical Sciences and Research (PSR)
Lister, I Nyoman Ehrich
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Nephroprotective Effect of Ethanolic Extract of Balakka (Phyllanthus emblica L.) on Rats Induced Ethylene Glycol and Ammonium Chloride Girsang, Ermi; Halima, Piyanto; Nasution, Ali Napiah; Lister, I nyoman Ehrich; Lie, Sukirman
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 2 No. 2 (2019): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (814.27 KB)

Abstract

The kidneys are a pair of symmetrical organs that function to filter the blood and are located in the retroperitoneal region on the posterior wall of the abdomen. The kidneys are drained about 25% of cardiac output. Kidney damage due to the accumulation of kidney stones can be caused by ethylene glycol substances to form calcium oxalate crystals (CaC2O4). This crystal is common in urine specimens even in healthy animals. Crystal formation can be accelerated by the administration of ammonium chloride. This study aims to determine the nephroprotective effects of balakka ethanol extract to reduce creatinine and urea levels in rats induced by ethylene glycol and ammonium chloride. The results showed that the balakka ethanol extract with a dose variation of 50 mg/kg body weight, 100 mg/kg body weight, and 150 mg/kg body weight had nephroprotective activity in rats that had been induced by ethylene glycol and ammonium chloride. Where the induction group had the largest serum creatinine mean of 1.43 mg/dl. The EEB treatment group dose of 50 mg / kgBB had a serum creatinine value of 1.01 mg / dl. The EEB treatment group dose of 100 mg / kgBB had a serum value of 0.91 mg / dl. The balakka ethanol extract treatment group dose of 150 mg / kgBB had a serum value of 0.73 mg / dl. The induction group had the largest serum urea serum of 76.56 mg/dl. EEB treatment group dose of 50 mg / kgBB had serum urea value of 63.36 mg / dl. The EEB treatment group dose of 100 mg / kgBB had a serum urea value of 49.73 mg / dl. The balakka ethanol extract treatment group dose of 150 mg / kgBB had a serum urea value of 49.90 mg / dl. Key Word : ureum, creatine, kidney.
Comparison of Antioxidant and Anti-hyaluronidase Potentials of Pineapple Core Extract (Ananas comosus (L.) Merr.) and Luteolin Jusri, Ridza; Widodo, Wahyu Setia; Widowati, Wahyu; Armansyah, Adek; Sormin, Delores Elisabeth; Fachrial, Edy; Lister, I Nyoman Ehrich
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n2.1629

Abstract

Skin aging processes are divided into chronological aging and premature aging. Premature aging is generally caused by free radicals, from both air pollution and photoaging. Natural compounds from plant extracts are among sources of antioxidants and anti-hyaluronidase which have the ability to prevent antiaging. One of the potential fruits related to antioxidant and antiaging activities is Anana scomosus. A. comosus has a number of phenolic compounds with biological activities. One of the main phenolic compounds in A. comosus is luteolin. The aim of this study was to evaluate the antioxidant and antiaging potentials of Pineapple Core Extract (PCE). This study was conducted at the Biomolecular and Biomedicine Research Center, Aretha Medika Utama from August to November 2018. Analysis of antioxidants from PCE and luteolin was carried out using H2O2 scavenging activity assay. The antiaging assay was carried out through inhibition of hyaluronidase enzyme, one of the important enzymes in the aging process. Luteolin had lower IC50 value of H2O2 scavenging activity of around 24.12±3.13 μg/ml, which was better than CPE with IC50 of 304.56±3.76μg/mL. The results of hyaluronidase inhibition activity assay showed that luteolin compound had a lower IC50 value of 67.38±3.99 μg/mL when compared to PCE with an IC50 value of 161.15±1.05 μg/mL. Hence, Luteolin has higher antioxidant and anti-hyaluronidase activities than PCEPerbandingan Aktivitas Antioksidan dan Antihialuronidase Ekstrak Bonggol Buah Nanas (EBN) dengan Senyawa LuteolinTerdapat dua jenis proses penuaan kulit, penuaan kronologis dan penuaan dini. Penuaan dini umumnya disebabkan oleh radikal bebas, baik dari polusi udara maupun photoaging. Penggunaan antioksidan dan antihialuronidase untuk mencegah proses penuaan telah banyak diketahui. Ananascomosus merupakan salah satu tanaman yang memiliki potensi sebagai antipenuaan dan antioksidan karena mengandung sejumlah senyawa fenolik yang memiliki berbagai aktivitas biologis. Salah satu senyawa fenolik utama dalam A. comosus adalah luteolin. Tujuan penelitian ini adalah untuk mengevaluasi potensi antioksidan dan anti-aging dari Ekstrak Bonggol Nanas (EBN) dan senyawa pembandingnya yaitu luteolin . Penelitian ini dilakukan di Biomolecular and Biomedicine Research Center, Aretha Medika Utama dari Agustus hingga November 2018. Pengujian antioksidan dari EBN dan luteolin  dilakukan menggunakan uji pemerangkapan H2O2. Uji antipenuaan dilakukan melalui uji penghambatan aktivitas hialuronidase, salah satu enzim penting dalam proses penuaan. Hasil penelitian menunjukkan bahwa Luteolin  memiliki nilai IC50 aktivitas pemerangkapan H2O2lebih rendah sekitar 24.12±3.13 μg/ml lebih baik daripada EBN dengan IC50 sebesar 304.56±3.76μg/mL. Hasil uji aktivitas penghambatan hialuronidase, senyawa luteolin  memiliki nilai IC50 lebih rendah, yaitu sebesar 67,38±3,99 μg/mL dibanding dengan EBN nilai IC50 161,15±1,05μg/mL. Hasil ini menunjukkan bahwa senyawa pembanding luteolin  memiliki aktivitas antioksidan dan antihialuronidase yang lebih tinggi daripada EBN. 
Antioxidant and Antityrosinase Activities of Ethanolic Pachyrhizuserosus Peel and Tuber Extract Siregar, Irma Dolsyeria; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Marpaung, Hans Hotma; Ferdinand, Sahna; Fachrial, Edy; Lister, I Nyoman Ehrich
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.515 KB) | DOI: 10.15395/mkb.v51n2.1628

Abstract

Aging process is a physiological process in living organisms caused by, among others, free radicals. One of the free-radical-related aging problems is skin hyperpigmentation (excessive melanin) due to increasing tyrosinase enzyme activities. Natural compounds are widely used as antioxidant and antiaging agents. Bengkuang (Pachyrhizuserosus) is known as a source of various active compounds which can be used against free radicals to reduce the risk of skin aging  through  tyrosinase enzyme inhibition. This study was performed in September 2018 in Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, Indonesia to examine the antioxidant and antityrosinase properties of Pachyrhizuserosus peel extract (PPE) and Pachyrhizuserosus  tuber extract (PTE).The extraction of PPE and PTE was performed using 70% ethanol by maceration method, followed by  phytochemical analysis using modified Farnsworth method. Antioxidant activities were measured through 2,2-Diphenyl-1-picrylhydrazil (DPPH) scavenging activities while antiaging assay were conducted through the  tyrosinase activity inhibition. In this study, PPE contained saponin, tannin, triterpenoid, and terpenoid while the PTE showed the presence of flavonoid, saponin, phenol, tannin, and alkaloid in phytochemical analysis. In the antioxidant assay, PPE presented a higher DPPH scavenging activities (IC50= 84.09 µg/mL) when compared to PTE (IC50= 98.30 µg/mL)(p<0.05). In antiaging assay, PPE showed a higher tyrosinase inhibitory activities when compared to PTE with =97.05µg/mL and 194.51µg/mL,respectively. It can be concluded that PPE has antioxidant and antiaging activities effective for preventing skin aging. Aktivitas Antioksidan dan Antitirosinase Ekstrak Etanol Kulit dan Daging PachyrhizuserosusProses penuaan merupakan suatu proses fisiologis yang terjadi pada makhluk hidup yang dapat disebabkan oleh radikal bebas. Hiperpigmentasi kuli tmerupakan salah satu masalah penuaan yang disebabkan oleh radikal bebas melalui peningkatan aktivitas enzim tirosinase. Bengkuang (Pachyrhizus erosus) diketahui mengandung berbagai senyawa aktif yang dapat menangkal radikal bebas serta mengurangi risiko penuaan kulit. Penelitian dilaksanakan pada September 2018 di Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, Indonesia untuk menguji kemampuan aktivitas antioksidan dan antitirosinase pada ekstrak etanol kulit bengkuang (EEKB) dan ekstrak etanol daging bengkuang (EEDB). Pembuatan ekstrak kulit dan daging bengkuang dilakukan dengan cara mengekstraksi bahan dengan etanol 70% menggunakan metode maserasi, kemudian dilanjutkan dengan analisis fitokimia ekstrak dengan modifikasi metode Farnsworth. Aktivitas antioksidan diuji dengan mengukur pemerangkapan 2,2-Diphenyl-1-picrylhydrazil (DPPH) sedangkan antiaging diuji dengan mengukur aktivitas penghambatan tirosinase. Pada uji fitokimia menunjukkan EEKB memiliki kandungan senyawa saponin, tanin, triterpenoid dan terpenoid, sedangkan EEDB menunjukkan kandungan senyawa flavonoid, saponin, fenol, tanin, dan alkaloid. Pada uji antioksidan, EEKB memiliki aktivitas tertinggi pada pemerangkapan DPPH (IC50=84.09 µg/mL) dibanding dengan EEDB (IC50=98.30 µg/mL) (p≤0.05). Pada pengujian antiaging, EEKB memiliki nilai yang lebih tinggi pada aktivitas penghambatan tirosinase dibandingkan dengan EEDB (IC50=97.05 µg/mL; 194.51 µg/mL (p≤0.05). Simpulan, EEKB memiliki aktivitas antioksidan dan antiaging sehingga efektif dalam mencegah penuaan kulit. 
Comparison of Antioxidant and Anti-Tyrosinase Activities of Pineapple (Ananas comosus) Core Extract and Luteolin Compound Vrianty, Dela; Qodariah, Rismawati Laila; Widowati, Wahyu; Sinaga, Ade Putra Fratama; Fibrina, Dewi; Fachrial, Edy; Lister, I Nyoman Ehrich
Jurnal Kedokteran Brawijaya Vol 30, No 4 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.679 KB) | DOI: 10.21776/ub.jkb.2019.030.04.2

Abstract

Free radicals and UV exposure can cause aging. Aging prevention needs substances that can prevent molecular oxidation reactions in cells and inhibit the activity of enzymes that trigger aging. Research on pineapple skin and flesh extract (Ananas comosus (L.) Merr.) reported the presence of luteolin compound which functions as antioxidants and antityrosinase. However, in this study, the object used was pineapple core extract (PCE), which has not been widely known for its antioxidant and antityrosinase activity. Therefore, the purpose of this study was to determine the content of phytochemical compounds, antioxidant activity, and inhibitory activity of tyrosinase enzymes by PCE and then compared with the luteolin (LT) compound test results using Fansworth method, DPPH scavenging activity assay and tyrosinase enzyme inhibition assay. Based on the study results, the phytochemical compounds contained in PCE were tannins and triterpenoids.  PCE and LT produced the highest DPPH scavenging activity, which was 64.86% and 59.32% (final concentration 200μg/ml and 6.25μg/ml) and the highest tyrosinase inhibition activity, which was 60.52% and 85.02% (final concentration 100 μg/ml). Antioxidant activity was determined based on IC50 of 87.46µg/mL and 4.17 µg/ml respectively. IC50 tyrosinase enzyme inhibition EBN and LT respectively at 62.27µg/ml and 5.25 µg/ml. Antioxidant activity through DPPH free radical scavenging test and tyrosinase enzyme inhibition activity by PCE was lower than LT.
Antioxidant and Anti-Hyaluronidase Activities of Dragon Fruit Peel Extract and Kaempferol-3-O-Rutinoside Liana, Liana; Rizal, Rizal; Widowati, Wahyu; Fioni, Fioni; Akbar, Khainir; Fachrial, Edy; Lister, I Nyoman Ehrich
Jurnal Kedokteran Brawijaya Vol 30, No 4 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.286 KB) | DOI: 10.21776/ub.jkb.2019.030.04.3

Abstract

Aging is a natural process in human life and is triggered by the presence of free radicals (ROS). The use of antioxidants from natural ingredients is one of the breakthroughs to overcome aging and counteract the harmful effects caused by the free radicals. This study aimed to determine and compare the antioxidant activity of H2O2 scavenging and hyaluronidase inhibition of red dragon fruit peel extract (DFPE) and kaempferol-3-o-rutinoside (KOR) compounds. Dragon fruit peel extract (DFPE) is obtained through extraction by maceration method using 70% ethanol solvent. The design of this study included antioxidant and anti-aging activity assay of EKBN and KOR at the series concentration of 15.63; 31.25; 62.50; 125; 250; 500 µg/mL through H2O2 scavenging, as well as the DFPE and KOR hyaluronidase inhibition assay at the series concentration of 5.21; 10.42; 20.83; 41.7; 83.33; 166.67 µg/mL. EKBN shows that the average activity of H2O2 scavenging is lower than KOR. In addition, the IC50 values of KOR for H2O2 scavenging is lower (351.46±2.30ug/mL) than DFPE (409.64±23.17ug/mL). While, KOR also has higher values of inhibitory activity than of the DFPE. However, the IC50 value of KOR for hyaluronidase inhibition activity was 84.07±10.46µg/mL, equivalent to the IC50 value of DFPE (85.32±10.24µg/mL). The presence of antioxidant and anti-aging activity in the EKBN is probably caused by betalain and the KOR compound itself contained in red dragon fruit. The results of the paired-samples T-test on antioxidant activity and anti-aging of DFPE and KOR showed non-significant difference. Thus, DFPE has an equivalent antioxidant and anti-aging through H2O2 scavenging and hyaluronidase activity as possessed by the KOR compound.
Antioxidant Activities of Black Soybean Extract (Glycine max (L.) Merr.) and Daidzein as Hydroxyl and Nitric Oxide Scavengers Irwan, Mulia; Girsang, Ermi; Nasution, Ali Napiah; Lister, I Nyoman Ehrich; Amalia, Annisa; Widowati, Wahyu
Majalah Kedokteran Bandung Vol 52, No 2 (2020)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.086 KB) | DOI: 10.15395/mkb.v52n2.1816

Abstract

Free radicals are known as a leading factor in aging. Nitric oxide (NO) and O2•- have been shown to inhibit the synthesis of matrix components and stimulate prolidase activities involved in collagen degradation. Hydroxyl (•OH) radical is a precursor of hyaluronic acid degradation. Antioxidants act as a scavenger for free radicals, creating a possibility to use them to prevent the aging process. Black soybeans (Glycine max (L.) Merr.) contain bioactive compounds that have the ability to scavenge free radicals, including daidzein. Daidzein belongs to the isoflavones group which is the most active compound in black soybean. This study aimed to understand the antioxidant activities of the black soybean extract (BSE) and daidzein compound in the scavenging of •OH and NO radicals and was performed at the Aretha Medika Utama-Biomolecular and Biomedical Research Center (BBRC), Bandung, Indonesia, from September to November 2018. The scavenging activity of •OH was assessed using the deoxyribose method, while the assessment of the scavenging of NO radicals was carried out using the sodium nitroprusside method. BSE and daidzein had an •OH scavenging activity with an IC50 value of 71.07 μg/mL, followed by daidzein with 24.57 μg/mL. A higher NO scavenging activity was seen in BSE with an IC50 of 71.60 μg/mL followed by daidzein with 35.68 μg/mL. Daidzein has a higher antioxidant activity through hydroxyl and nitric oxide scavenging compared to Glycine max (L.) Merr. extract. Hence, daidzein has a higher potential as an anti-aging agent based on the free-radical theory of aging. Aktivitas Antioksidan Ekstrak Kedelai Hitam (Glycine max (L.) Merr.) dan Daidzein sebagai Pemerangkap Hidroksil dan Nitrogen MonoksidaRadikal bebas dikenal sebagai faktor utama penuaan. Nitrogen monoksida (NO) dan O2•- telah terbukti menghambat sintesis komponen matriks dan merangsang aktivitas prolidase yang terlibat dalam degradasi kolagen. Radikal hidroksil (•OH) adalah prekursor dari degradasi asam hialuronat. Antioksidan bertindak sebagai pemerangkap radikal bebas sehingga dapat digunakan untuk mencegah proses penuaan. Kedelai hitam (Glycine max (L.) Merr.) mengandung senyawa bioaktif yang mampu memerangkap radikal bebas, salah satunya adalah daidzein. Daidzein termasuk dalam kelompok isoflavon yang merupakan senyawa paling aktif dalam kedelai hitam. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan, pemerangkapan •OH dan NO dari ekstrak etanol kedelai hitam (BSE) dan senyawa daidzein. Penelitian ini dilakukan selama bulan September sampai dengan November tahun 2018 di Laboratorium Aretha Medika Utama Biomolecular and Biomedical Research Center (BBRC) Bandung. Pemerangkapan •OH dilakukan dengan metode deoksiribosa, sedangkan pemerangkapan radikal NO dilakukan dengan metode menghitung natrium nitroprusida. BSE dan daidzein memiliki aktivitas pemerangkapan •OH dengan nilai IC50 71,07 μg/mL diikuti oleh daidzein 24,57 μg/mL. Aktivitas pemerangkapan NO pada BSE ditunjukkan dengan IC50 71,60 μg/mL sementara daidzein 35,68 μg/mL. Daidzein memiliki aktivitas antioksidan yang lebih tinggi berdasarkan pemerangkapan •OH dan NO dibanding BSE. Oleh karena itu, daidzein memiliki potensi lebih sebagai antiaging menurut teori penuaan akibat radikal bebas.
AKTIVITAS INULINASE BAKTERI TERMOFILIK UTMSDA 6 YANG DIISOLASI DARI SUMBER AIR PANAS SIDEBUK DEBUK, SUMATERA UTARA Nabila, Pivi; Ainy, Al-Annisa Fadhila; Tarigan, Nuzulul Akbar; Fachrial, Edy; Lister, I Nyoman Ehrich
(Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) Vol 6, No 1 (2021): JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat)
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37887/jimkesmas.v6i1.16394

Abstract

Abstrak Indonesia masih membutuhkan impor untuk memenuhi kebutuhan enzim. Dibutuhkan 3 enzim untuk mengubahpati menjadi fruktosa dan menghasilkan 45% fruktosa. Enzim inulinase dinilai lebih baik karena membutuhkansatu tahap reaksi enzim dan menghasilkan 95% fruktosa. Bakteri termofilik dapat hidup hingga suhu 121°C dinilaimenjadi penghasil enzim termostabil yang efektif bagi pihak industri yang membutuhkan suhu tinggi. Tujuan daristudi ini mengisolasi dan karakterisasi bakteri termofilik penghasil enzim inulinase dari koleksi isolat lab biologimolekuler Universitas Prima Indonesia. Skrining inulinase menggunakan media Czapek Dox with 2% agar dan 1%inulin powder. Inkubasi selama 5-7 hari pada suhu 48-49°C. Hidrolisis inulin oleh koloni bakteri ditandai denganadanya zona bening setelah penuangan lugol iodine. Dari isolat yang diteliti dengan nama UTMSDA 6,karakterisasi secara biokimia menunjukan perubahan warna menjadi kuning-kuning, negatif pada tes hidrolisapati, tes keretakan media, tes sitrat, tes endapan hitam, serta tes hidolisa gelatin dan positif pada tes katalasedan tes motilitas. Tes morfologi menggunakan perwarnaan gram didapatkan batang gram-positif. Pengujianaktivitas enzim inulinase menggunakan spektrofotometer dengan panjang gelombang 570nm, didapatkaninkubasi jam ke-8 memiliki aktivitas inulinase paling tinggi sebesar 5,036 IU. Dari hasil penelitian, isolat memilikikemampuan sebagai sumber produksi inulinase termofilik yang berpotensi untuk digunakan di dunia industri.Keywords: Inulinase, bakteri termofilik, fruktosa, inulin
PENGARUH PENAMBAHAN PREBIOTIK INULIN TERHADAP AKTIVITAS ANTIBAKTERI LACTOBACILLUS CASEI Siregar, Hafiz Hawari; Silaban, Agnes Novica Putri; Sofiyana, Maria; Purba, Rosi Krisdayanti; Intisari, Yayo; Lister, I Nyoman Ehrich; Fachrial, Edy
Preventif Journal Vol 6, No 1 (2021): Preventif Journal
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37887/epj.v6i1.22594

Abstract

AbstrakInulin adalah salah satu prebiotik yang banyak dipergunakan sebagai sumber nutrisi bagi probiotik. Tujuan penelitian ini ialah untuk mengetahui efek penambahan prebiotik inulin pada aktivitas antibakteri Lactobacillus casei. Metode antibakteri dilakukan dengan metode difusi cakram. Dalam percobaan terpisah bakteri asam laktat yang telah diuji kemudian diinokulasi ke dalam tabung reaksi yang berisi MRS Broth 10 mL dan MRS Broth 10 mL yang telah ditambahkan dengan 5% tepung inulin. Kemudian diinkubasi dengan suhu 37 °C selama 24 jam.  Kemudian dilakukan kultur Bakteri patogen (Staphylococcus aureus) pada Nutrien Agar dengan cara mencelup kapas steril ke dalam tabung reaksi yang berisi bakteri patogen dan kemudian menyebarkannya secara merata pada Nutrien Agar steril dalam cawan petri. Nutrien Agar dalam cawan petri yang sudah diinokulasi pada suhu 37 °C selama 24 jam dengan bakteri patogen diinkubasikan. Hasil isolasi menunjukkan bahwa probiotik L. casei yang diisolasi dari minuman susu komersil memiliki aktivitas antibakteri spektrum luas. Probiotik L. casei ini efektif menghambat pertumbuhan bakteri S. aureus. Penambahan konsentrasi prebiotik inulin 0,5% (b/v) pada MRSB dapat menambah daya hambat bakteri Lactobacillus casei sebesar 8,4 mm terhadap pertumbuhan bakteri S.aureus. Untuk penelitan selanjutnya, perlu dilakukan pengamatan tentang berat molekul dan golongan bakteriosin yang dihasilkan oleh L. casei. Kata Kunci : Prebiotik, Inulin, Antibakteri, Lactobacillus casei
Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin Geeta,; Widodo, Wahyu Setia; Widowati, Wahyu; Ginting, Chrismis Novalinda; Lister, I Nyoman Ehrich; Armansyah, Adek; Girsang, Ermi
Pharmaceutical Sciences and Research
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Skin aging is a complex natural process caused by both intrinsic or genetically programmed aging and extrinsic aging caused by environmental factors, such as free radical. The use of antioxidant and anti-collagenase to prevent the aging proses has been known. Natural compounds from plants are one of the sources of antioxidant and anti-collagenase that has ability to prevent aging. Genistein and Epicatechin are the major phenolic compound found in G. max. Objectives: This research was to evaluate the antiaging potential of genistein and epicatechin through antioxidant activity assay (ABTS-reducing activity assay) and collagenase inhibitory activity assay. Methods: Antioxidant analysis of Genistein and Epicatechin was performed by 2,2’- azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) reducing activity Assay. Antiaging assay was conducted through inhibitory of collagenase, one of important enzyme in aging process. Results: ABTS- reducing activity assay showed that both compounds had great ABTS-reducing activity in which epicatechin had better activity than genistein. Epicatechin had low value of IC50 ABTS-reducing activity around 14.39 µg/ml better than genistein with IC50 about 43.17 µg/ml. In terms of collagenase inhibitory activity assay, epicatechin had lower value of IC50 (9.08+-3.46 ug/ml), better than genistein (98.74+-4.25 ug/ml). Conclusion: Epicatechin had higher antioxidant and anti- collagenase activity compared to Genistein.
Co-Authors Ainy, Al-Annisa Fadhila Annisa Amalia Armansyah, Adek Edy Fachrial Ferdinand, Sahna Fibrina, Dewi Fioni, Fioni Geeta, Ginting, Chrismis Novalinda Girsang, Ermi Halima, Piyanto Hanna Sari Widya Kusuma, Hanna Sari Widya Intisari, Yayo Irwan, Mulia Jusri, Ridza Khainir Akbar, Khainir Liana Liana Lie, Sukirman Marpaung, Hans Hotma Nabila, Pivi Nasution, Ali Napiah Purba, Rosi Krisdayanti Qodariah, Rismawati Laila Rizal Rizal Silaban, Agnes Novica Putri Sinaga, Ade Putra Fratama Siregar, Hafiz Hawari Siregar, Irma Dolsyeria Sofiyana, Maria Sormin, Delores Elisabeth Tarigan, Nuzulul Akbar Vrianty, Dela WAHYU WIDOWATI Widodo, Wahyu Setia