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PENGARUH EKSTRAK KULIT BATANG JAMBU METE (Anacardium occidentale Linn.) SEBAGAI BAHAN ANTISEPTIK TERHADAP EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) PADA LUKA BEKAS PENCABUTAN GIGI MARMUT: INFLUENCE OF CASHEW STEM BARK EXTRACT (Anacardium occidentale Linn.) AS AN ANTISEPTIC ON VEGF EXPRESSION AFTER TOOTH EXTRACTION OF THE GUINEA PIG Harsini; Iwa Sutardjo; Sudibyo Martono; Siti Sunarintyas; Sudarsono
Dentika: Dental Journal Vol. 18 No. 1 (2014): Dentika Dental Journal
Publisher : TALENTA

Pencabutan gigi dapat berdampak pada penghambatan kontinuitas jaringan yang memerlukan waktu untukkembali sembuh. Vascular Endhotelial Growth Factor (VEGF) merupakan salah satu mediator pro inflamasiyang berhubungan dengan pembentukkan pembuluh darah baru. Kulit batang jambu mete (Anacardiumoccidentale Linn.) mempunyai kandungan fenolik yang dapat berperan pada pengaturan fungsi sel denganmenginduksi VEGF. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi ekstrak kulit batang jambumete sebagai bahan antiseptik terhadap ekspresi VEGF pada luka dampak pencabutan gigi hewan coba(marmut/Guinea Pig). Kulit batang jambu mete diambil dari Mojolegi, Karangtengah, Imogri, Bantul. Ekstraksidilakukan secara maserasi dengan pelarut etanol 70%. Bahan antiseptik dibuat dalam konsentrasi 3%, 5% dan 7%. Sebanyak 48 ekor marmut jantan, umur sekitar 2,5-3 bulan dengan bobot badan rata-rata 250-300 grdibagi menjadi 4 kelompok dan setiap kelompok terdiri atas 12 ekor. Seluruh marmut dilakukan pencabutangigi insisivus bawah kanan. Kelompok I adalah kelompok kontrol yang ditetesi air suling. Kelompok II ditetesiekstrak kulit batang jambu mete dengan konsentrasi 3%. Kelompok III ditetesi ekstrak kulit batang jambu mete5% dan kelompok IV ditetesi ekstrak kulit batang jambu mete 7%. Dekapitasi tiap kelompok sebanyak 4 ekordilakukan pada hari ke 3, 7dan 14. Setelah dekapitasi, soket bekas gigi diambil dan dibuat preparat histologisnyadengan pengecatan imunohistokimia. Penghitungan ekspresi VEGF dilakukan dibawah mikroskop denganperbesaran 40x. Data yang diperoleh dianalisis menggunakan Analisis Varians dua jalan dilanjutkan denganLSD0,05. Hasil penelitian menunjukkan antar konsentrasi, antar hari, dan interaksi antar konsentrasi dan hariberpengaruh signifikan terhadap ekspresi VEGF (p< 0,05). Pada uji LSD diketahui terdapat perbedaan ekspresiVEGF yang signifikan antar kelompok konsentrasi dan kelompok hari. Sebagai kesimpulan, ekstrak kulit batangjambu mete dalam bahan kumur berpengaruh terhadap ekspresi VEGF.

Multiplikasi Tunas In Vitro Anggrek Phalaenopsis dan Analisis Keragaman Genetik dengan Marka SNAP Mira Humaira; Agus Purwito; Sudarsono; Dewi Sukma
Indonesian Journal of Agronomy Vol. 48 No. 1 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Clonal propagation of orchid by in vitro culture is needed to obtain the high quantity and quality of orchid propagules. This study aimed to understand the effect of BAP (benzyl amino purine) concentrations on the shoot multiplication of three Phalaenopsis hybrid populations and to analyze genetic diversity using Pto-SNAP markers on clonal plantlets. The experiments were performed separately for each population, namely shoots from seed germination of Phal. amabilis x Phal. ‘KHM 421’ with five level of BAP (0.00, 11.09, 22.19, 33.29, 44.39 µM), clonal shoots from Phal. ‘Salu Spot’ x Phal. bellina ‘1102-38’, and Phal. ‘Salu Spot’ x Phal. bellina ‘1102-44’ with four level of BAP (11.09, 22.19, 33.29, 44.39 µM). The results showed that the optimum BAP concentration for shoot multiplication on three population was 22.19 µM which shoot number obtained were 3.7, 4.7, and 6.0 at 12 week after planting (WAP). Protocorm like bodies (PLBs) was only produced on Phal. amabilis x Phal. ‘KHM 421’ population, which the highest number of PLBs was on 11.09 BAP. Evaluation of genetic diversity using 11 Pto-SNAP (single nucleotide amplified polymorphism) marker was performed on Phal. ‘Salu Spot’ x Phal. bellina ‘1102-44’ shoots. The results revealed the presence of somaclonal variation with 7.7% of genetic diversity of the shoots from BAP 22.19 µM treatment at Pto-241 and 11.1% from BAP 44.39 µM treatment at Pto-424. Keywords: clonal propagation, cytokinin, molecular markers, orchid, Pto, somaclonal variation

Production Technology for Kopyor Coconut Seednuts and Seedlings in Indonesia Hengky Novarianto; Ismail Maskromo; Sudarsono
International Coconut Community Journal Vol 30 No 2 (2014): CORD
Publisher : International Coconut Community

Kopyor coconut is a naturally-occurring mutant having fluffy solid endosperm in stead of the normal one. Similar type of coconut has been found in several other Asian countries, with their distinct local names, such as Macapuno (Philippines), Makhrao Kathi (Thailand), Dikiri Pol (Sri Lanka), Thairu Thengai (India). It is a delicacy coconut to Indonesian and sells as much as ten times higher than normal coconut. In nature, three types of kopyor coconut exist in Indonesia: kopyor tall, kopyor dwarf, and kopyor hybrid. There are three kopyor dwarf varieties (‘Pati Kopyor Green Dwarf’, ‘Kopyor Yellow Dwarf’ and ‘Kopyor Brown Dwarf’) officially released, and one registered tall variety (‘Puan Kalianda Kopyor Tall’). In general, kopyor fruit yield under natural conditions is only < 25% of the total harvested fruits for both the tall and the dwarf types. Traditionally, Indonesian farmers harvest kopyor fruits at ten months after pollination while normal fruits at 11 months. They use the harvested normal fruits for propagation; but cannot guarantee whether or not they would produce korpyor fruit. Adoption of kopyor seedling production through embryo culture has been done. However, the seedling production is slow, while the price is very expensive, thus unaffordable to common farmers. Indonesian Palm Research Institute and Bogor Agricultural University have collaborated to develop alternative approaches to increase kopyor fruit production through the production of seedling that would ensure to produce korpyor fruit through control pollination. Initiated since 2010, the activities successfully overcome uncertainty in kopyor seedling production. Moreover, hybridization among local superior coconut varieties and known kopyor one have been done to broaden genetic background of kopyor trait and to develop breeding population for new kopyor varieties in the future. Overview and update of research progress on korpyor in Indonesia are presented in this paper.

Productivity of Three Dwarf Kopyor Coconut Varieties from Pati, Central Java, Indonesia Ismail Maskromo; Sudarsono
International Coconut Community Journal Vol 29 No 2 (2013): CORD
Publisher : International Coconut Community

Pati Dwarf Kopyor coconut originated from Pati, Central Java is one of the Kopyor coconut populations from Indonesia. Three varieties of Pati Dwarf Kopyor coconut, namely: Kopyor Green Dwarf, Kopyor Brown Dwarf and Kopyor Yellow Dwarf out of six potential varieties have been officially released as local superior varieties by the Minister of Agriculture, Republic of Indonesia at the end of 2010. The objectives of this research were to evaluate the stability of Kopyor fruit production among provenances of Pati Dwarf Kopyor coconuts. Evaluations were conducted especially for the three recently released varieties of Kopyor coconuts. Observations were carried out in 2011 and the collected data were then compared with Kopyor fruit production data collected in 2009 -2010. In three different locations, representatives of 10 Kopyor coconut provenances for each Kopyor variety were observed; therefore, a total of 90 trees were evaluated in this experiment. Observations were conducted for number of total bunches per palm, number of total fruits/per bunch, and the number of harvested Kopyor coconut fruits/per bunch. Three bunches were selected for each of the evaluated provenance. Results of the observation indicated that the observed yield and yield parameters of three Pati Dwarf Kopyor coconut varieties fluctuated by the year of observations. However, in general the observed yield and yield parameters among three varieties were similar. The average number of total bunches observed among three varieties evaluated ranged from 9.6-12.1 bunches per palm; the average number of fruits/per bunch ranged from 5.3-12.4; and the average number of harvested Kopyor fruits/per bunch ranged from 2.0-3.8. The estimated number of total fruits/palm/year among the three varieties ranged from 53.8-128.1 and the percentage of Kopyor fruit production observed among the three varieties evaluated ranged from 24.8-38.9 %. The estimated number of total harvested Kopyor fruits/palm/year ranged from 20.5-39.2, respectively. Rainfall pattern might affect directly or indirectly toward yield and yield components of three Pati Dwarf Kopyor coconut varieties.

ANALISIS MODEL PENGEMBANGAN KURIKULUM SISTEM FULL DAY SCHOOL DI SMP NEGERI SE-KECAMATAN LIMBOTO KABUPATEN GORONTALO Imam Mashudi; Sudarsono
International Interdisciplinary Conference on Sustainable Development Goals Vol 1 No 1 (2018): Proceedings of International Interdisciplinary Conference on Sustainable Developm
Publisher : LP2M Universitas Bina Mandiri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.192 KB)

This research aims to know the Curriculum Development Model of Full Day School System in State Junior High School All of Limboto Sub-districts, Gorontalo District, and to know the supporting and inhibiting factors of Curriculum Development of Full Day School System in State Junior High School All of Limboto Sub-districts, Gorontalo District. This research uses a qualitative approach which will be implemented in State Junior High School All of Limboto Sub-districts, Gorontalo District. Data collection techniques used are observation technique, interviews and documentation, with the data analysis technique used is an interactive model analysis. This research produces several points, namely: (1) Curriculum of Full Day School System implemented in the form of literacy activities which starts before teaching and learning activities and after teaching and learning activities (KBM) are scheduled with extracurricular activities, namely scouts, arts and sports according to what students are interested in. (2) supporting factors this Full Day School System more to put forward a few points namely can shape the students' character, Extracurricular activities are more effective, can help students doing homework rather than to playing outside, and can help students more discipline. Whereas, for the inhibiting factor of this system is sports and art facilities incomplete and literature books are still lacking

IMPLEMENTASI FUNGSI LEGISLASI DPRD DALAM PEMBENTUKAN PERATURAN DAERAH (KAJIAN DI BADAN PEMBENTUKAN PERATURAN DAERAH DPRD PROVINSI KALIMANTAN TENGAH): The Implementation of Legislation Function of Regional Representative Council in the Formation of Regional Law (a Study in Regional Law Formation Committee of Central Kalimantan Regional Representative Council) Sudarsono; Muhammad Riban Satia; Muhammad Yusuf
Pencerah Publik Vol. 9 No. 2 (2022): Pencerah Publik
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pencerah.v9i2.4256

The legislative function is one of the functions attached to the Regional Representative Council whose main task is to compile regional legal products in the form of Regional Law. The legislative function of this Regional Representative Council needs to be optimized because it is a representation of policy formulation based on the aspirations of the people (bottom-up policymaking). This study aims to examine how the implementation of the legislative function inherent in the Central Kalimantan Regional Representative Council in the process of forming Regional Law and the obstacles that occured. This study uses qualitative methods with data collection techniques through in-depth interviews and observation. The results of the study show that the Central Kalimantan Regional Representative Council carries out its legislative function through the stages of forming Regional Law. Some of the problems found in the process of forming the Regional Law were the absence of a Regional Law Needs Analysis. The other obstacles found were: 1) communication problems with other stakeholders; 2) the organizational structure in Regional Representative Council is inadequate in overseeing various fields; 3) the condition of human resources, including the members, staff and experts who still do not support the optimization of the legislative function; and 4) the role of the Regional Law Formation Committee which still does not have a main place in the Regional Representative Council.

PERLINDUNGAN HUKUM HAK WARIS ISTRI KEDUA DARI PERKAWINAN TIDAK TERCATAT DIKAITKAN DENGAN FUNGSI PENCATATAN PERKAWINAN: PERLINDUNGAN HUKUM HAK WARIS ISTRI KEDUA DARI PERKAWINAN TIDAK TERCATAT DIKAITKAN DENGAN FUNGSI PENCATATAN PERKAWINAN Sudarsono; Asmuni; TENGKU ERWINSYAHBANA
JURNAL HUKUM DAS SOLLEN Vol 9 No 1 (2023): Jurnal Hukum Das Sollen
Publisher : Fakultas Hukum Universitas Islam Indragiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32520/das-sollen.v9i1.2385

Tujuan penelitian untuk mengkaji dan menganalisis perlindungan hukum hak waris istri dari perkawinan tidak tercatat dikaitkan dengan fungsi pencatatan perkawinan dengan studi komparatif Fiqih Islam dan Undang-Undang No 1 Tahun 1974. Metode penelitian penulisan ini adalah penelitian yuridis normatif yang merujuk pada Al-Qur,an, Al- Hadist, Kitab-Kitab Fiqih, Peraturan Perundang-undangan dan buku terkait yang relevan dengan penelitian ini. Tekhnis analisis data yang digunakan ialah study kepustakaan yang dianalisis secara kualitatif. Hasil dari penelitian ini ialah bahwa Hak waris istri kedua dari perkawinan yang tidak tercatat dikaitkan dengan fungsi pencatatan perkawinan mempunyai hak atas harta waris yang dimilikinya bersama atas harta peninggalan berupa harta bawaan suami dan harta bersama yang didapatkan oleh istri kedua dan suaminya selama dalam masa perkawinan demi rasa keadilan dan perlindungan hukum, perlindungan hukum terhadap hak waris istri kedua dalam penelitian ini bahwa untuk mendapatkan hak waris serta pengakuan untuk melakukan pengesahan pernikahan ke pengadilan agama. Hasil itsbat nikah sebagai landasan hukum pencatatan perkawinannya kepada pegawai pencatat nikah pada Kantor Urusan Agama, untuk selanjutnya dikeluarkan Buku Kutipan Akta Nikah sebagai bukti autentik bahwa suatu perkawinan telah tercatat. Maka perlindungan hukum dan kepastian hukum terhadap hak waris istri kedua dari perkawinan yang tidak tercatat dikaitkan dengan fungsi pencatatan perkawinan dapat memperoleh hak waris secara hukum.

ANALISIS IMPLEMENTASI KEBIJAKAN PROGRAM BANTUAN BENIH JAGUNG HIBRIDA DI KABUPATEN GORONTALO Titin Dunggio; Sudarsono
Journal of Economic, Business, and Administration (JEBA) Vol. 4 No. 2 (2023): Journal of Economic, Business and Administration (JEBA)
Publisher : LP2M Universitas Bina Mandiri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47918/jeba.v4i2.5

Tujuan penelitian ini adalah: 1) untuk mengetahui implementasi ke-bijakan program bantuan benih jagung hibrida di Kabupaten Gorontalo; 2) untuk mengetahui faktor penghambat implementasi kebijakan-prog-ram bantuan benih jagung hibrida di Kabupaten Gorontalo. Penelitian ini menggunakan pendekatan kualitatif dan jenis peneli-tian deskriptif dengan menggunakan teknik pengumpulan data melalui observasi, wawancara mendalam dan dokumentasi. Teknik analisis data dilakukan melalui tahapan reduksi, penyajian & penarikan kesimpulan. Hasil penelitian ini menunjukkan bahwa: 1) implementasi kebijak-an Program Bantuan Benih Jagung Hibrida di Kabupaten Gorontalo be-lum berjalan secara optimal, yaitu terbatasnya sumber daya manusia dan dan sumber daya anggaran, standar operasional prosedur yang telah di atur oleh Menteri Pertanian masih perlu penyesuaian SOP di tingkat daerah karena terdapat beberapa SOP masih perlu kesepakatan dengan dinas terkait di tingkat provinsi, serta pemahaman masyarakat khusus-nya kelopmpok tani terhadap kebijakan ini masih sangat minim; 2) ham batan implementasi kebijakan Program Bantuan Benih Jagung Hibrida di Kabupaten Gorontalo yaitu keterbatasan sumber daya manusia dan sumber anggaran pendukung serta kurangnya pemahaman masyarakat khususnya kelompok tani terahadap kebijakan ini masih sangat minim

IMPLEMENTATION OF PLACING TRANSMIGRATION CITIZENS IN CREATING AN INDEPENDENT COMMUNITY IN AYUMOLINGO VILLAGE, PULUBALA DISTRICT, GORONTALO REGENCY abdurahman; sudarsono; Yosef p. Koton
Journal of Economic, Business, and Administration (JEBA) Vol. 4 No. 2 (2023): Journal of Economic, Business and Administration (JEBA)
Publisher : LP2M Universitas Bina Mandiri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47918/jeba.v4i2.424

ABSTRACTThis study aims to determine the implementation of the placement oftransmigration residents in realizing an independent society in AyumolingoVillage, Pulubala District, Gorontalo Regency and the factors that hinder theimplementation of Transmigration Residents' placement in creating anIndependent Society in Ayumolingo Village, Pulubala District, andGorontalo Regency.Research method used is a qualitative research method with data collectiontechniques including: 1) observation; 2) interview; and 3) documentation.Activities in data analysis, namely: 1) data reduction stage; 2) datapresentation stage; and 3) data verification stage.The results in this study are the implementation of the placement oftransmigration residents from planning has not been maximized because itdoes not include or involve residents around the transmigration area or areaas required and the implementation is not optimal because it still causes socialjealousy of local residents and complaints from transmigration residentsbecause the land is determined/given by the government is not in accordancewith what is in the location of the transmigration area and the supervisionhas not been effective, so that there are transmigration residents whohave been placed away from their homes/settlement locations, so that thegovernment's goal in an effort to achieve business independence and maketransmigration residents feel at home or settle in the location of the transmigrationarea has not can be realized. As for the factors that hinder the implementationof the placement of Transmigration Citizens in AyumolingoVillage, Pulubala District, Gorontalo Regency, namely commitment is stilllow, transparency is still lacking and human resources are still limited, bothimplementing apparatus resources and community resources, thus hamperingthe implementation and implementation placement of transmigrants.

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