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EFEKTIFITAS PENGIKATAN LOGAM Pb OLEH BAKTERI, Bacillus subtilis Maulin Inggraini
JURNAL SAINS NATURAL Vol. 4 No. 2 (2014): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.832 KB) | DOI: 10.31938/jsn.v4i2.87

Abstract

Effectiveness of Heavy Metal (Pb) Binding of Bactery Bacillus subtilis        The rapid development of technology and industries lead to higher environmental pollution by heavy metals, especially Pb. Reduction of heavy metals is commonly done using chemical and physical approach. Another alternative is biological technique which is more efficient due to its higher metal binding and readily available as materials. Bacillus subtilis was a potential bacterium in reducing heavy metal contamination. This study aimed to determine the B. subtilis binding effectiveness of Pb; to test the bacterial tolerance to Pb at varied concentrations; and to detect the accumulation sites of Pb within  B. subtilis. The results showed that B. subtilis was able to bind Pb with the effectiveness percentage  of 17.45%. Pb was accumulated in the cell walls.Keyword: Bacillus subtilis, Pb binding  ABSTRAK         Pesatnya perkembangan teknologi dan industri menyebabkan pencemaran lingkungan yang lebih tinggi dengan logam berat, terutama Pb. Pengurangan logam berat yang biasanya dilakukan dengan menggunakan bahan kimia dan pendekatan fisik. Alternatif lain adalah teknik biologi yang lebih efisien karena logam yang lebih tinggi mengikat dan tersedia sebagai bahan. Bacillus subtilis adalah bakteri potensial dalam mengurangi kontaminasi logam. Penelitian ini bertujuan untuk mendeterminasi B. subtilis yang efektivitas mengikat Pb; untuk menguji toleransi bakteri terhadap Pb pada konsentrasi yang bervariasi; dan untuk mendeteksi akumulasi Pb dalam Bacillus subtilis. Hasil penelitian menunjukkan bahwa B. subtilis mampu mengikat Pb dengan efektivitas persentase 17,45%. Pb terakumulasi dalam dinding sel.Kata Kunci : Bacillus subtilis, pengikatan Pb
STATUS KUALITAS AIR TANAH DI KELURAHAN PEDURENAN, KECAMATAN MUSTIKAJAYA, BEKASI TIMUR Maulin Inggraini; Siti Nurfajriah; Pangeran Andareas
JURNAL SAINS NATURAL Vol. 6 No. 2 (2016): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.173 KB) | DOI: 10.31938/jsn.v6i2.161

Abstract

Status of  Ground Water Quality in The Village of Pedurenan, Mustikajaya District, East BekasiSanitation and poor hygiene practice and unsafe drinking water contributes to 88% of childhood deaths from diarrhea in worldwide. For children who survive often suffer from diarrhea contribute to nutritional problems, preventing children to be able to achieve their maximum potential. According to the Basic Health Research (Riskesdas) in 2013, the incidence and prevalence of diarrhea for all age groups in Indonesia is 3,5% and 7,0%. Respectively the incidence of diarrhea is closely associated with the quality of sanitation and groundwater used by society. This study aimed to determine the quality of water used for sanitation especially groundwater, in Sub Pedurenan, District Mustikajaya, East Bekasi. Results showed that groundwater quality inspection using the Most Probable Number (MPN) in RW 02, Village and District Pedurenan Mustikajaya, there were 4 samples were negative of coliform, and 25 coliform positive samples, with the highest number of 1100 cells / 100 mL. This indicates that ground water for sanitation and groundwater of sampling locations are generally contaminated by coliform bacteria.Keywords: groun water, coliform, diarrhea, water quality, MPN ABSTRAK Sanitasi dan perilaku kebersihan yang buruk serta air minum yang tidak aman berkontribusi terhadap 88% kematian anak akibat diare di seluruh dunia. Bagi anak-anak yang bertahan hidup, seringnya menderita diare berkontribusi terhadap masalah gizi, sehingga menghalangi anak-anak untuk dapat mencapai potensi maksimal mereka. Menurut hasil Riset Kesehatan Dasar (Riskesdas) tahun 2013, insiden dan prevalensi diare untuk seluruh kelompok umur di Indonesia adalah 3,5% dan 7,0%. Kejadian diare sangat erat kaitannya dengan kualitas sanitasi dan air tanah yang digunakan masyarakat. Penelitian ini bertujuan untuk mengetahui kualitas air yang digunakan untuk sanitasi serta air tanah khususnya di Kelurahan Pedurenan, Kecamatan Mustikajaya, Bekasi Timur. Hasil menunjukkah bahwa pemeriksaan kualitas air tanah menggunakan metode Most Probable Number (MPN) di RW 02 Kelurahan Pedurenan dan Kecamatan Mustikajaya, terdapat 4 sampel yang negatif coliform, dan 25 sampel positif coliform, dengan jumLah tertinggi 1100 sel/100 mL. Hal ini mengindikasikan bahwa air tanah untuk sanitasi dan air tanah di lokasi pengambilan sampel umumnya tercemar oleh bakteri coliform.Kata Kunci: Air tanah, coliform, diarrhea, Kualitas Air, MPN
Pola Resistensi Bakteri Penyebab Infeksi Saluran Kemih (ISK) pada Pasien di Salah Satu Rumah Sakit Swasta di Jakarta Utara Periode 2019-2021 Maulin Inggraini; Reza Anindita; Euodia Naomi Septiana Siburian; Noor Andryan Ilsan
Jurnal Kesehatan Islam : Islamic Health Journal Vol 11, No 1 (2022): Jurnal Kesehatan Islam : Islamic Health Journal
Publisher : Publikasi oleh Fakultas Kedokteran Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jki.v11i1.16080

Abstract

Urinary Tract Infection (UTI) is an infectious disease of the urinary tract caused by bacteria. Treatment therapy for UTI is done by giving antibiotics. However, the use of antibiotics often poses a risk of bacterial resistance that causes UTIs which has an impact on increasing morbidity rates, resulting in high UTI treatment costs. This study aims to determine the pattern of UTI bacterial resistance to antibiotics in UTI patients at a private hospital in North Jakarta for the 2019-2021 period. Cross sectional design, using a non-analytic retrospective approach conducted at a private hospital in North Jakarta for the 2019-2021 period. The sample of this study was secondary data totaling 159 urine of UTI patients taken by purposive sampling. The data is processed in tabular form and described to see a picture of UTI patients in a private hospital in North Jakarta for the 2019-2021 period. The results showed that there were more female UTI patients (68%) than men (32%), the most common bacteria causing UTI was E. colli (61.64%), the dominant antibiotic causing UTI bacterial resistance was ampicillin (67.92%). The conclusion of this study is that E. coli is the most dominant cause of UTI and ampicillin is the antibiotic that causes the most resistance to UTI bacteria.
Identifikasi Telur Soil Transmitted Helminth (STH) Pada Kubis (Brassica oleracea) dan Kemangi (Ocimum basilicum) Di Penjual Makanan Sepanjang Jalan Dasa Darma Kecamatan Rawalumbu Kota Bekasi Reza Anindita Anindita; Rosa Ihza Arlinda; Maulin Inggraini
JURNAL BIOSHELL Vol 11 No 1 (2022): April
Publisher : Pendidikan Biologi,Universitas Islam Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56013/bio.v11i1.1352

Abstract

Worm infections caused by STH in Indonesia have a prevalence of 50%. One of the spread of STH occurs through soil or fertilizer contaminated with STH. If the soil or fertilizer contaminates vegetables, then the vegetables are potentially contaminated with STH eggs. If these vegetables are consumed by humans, they have the potential to cause diarrhea. This study aims to screen for the presence of STH eggs in vegetables commonly consumed by the public as fresh vegetables, namely cabbage (Brassica oleracea) and basil (Ocimum basilicum). This type of research is descriptive quantitative with cross sectional design. The samples in this study were 5 samples of basil and 5 samples of cabbage, each sample taken from 5 sellers of pecel catfish and grilled chicken along Dasa Darma street, Kec, Rawalumbu, Bekasi City. The sampling technique was done by purposive sampling. Data analysis was carried out with a quantitative descriptive approach. The results of this study showed that 5 samples of basil (100%) were positive for Hookworm eggs while all samples of cabbage were negative for Hookworm eggs. The conclusion of this study is that the use of the sedimentation method can be used as an early detection technique for the presence of Hookworm eggs in basil sold by pecel catfish and grilled chicken sellers along Dasa Darma street, Rawalumbu, Bekasi City. Keywords: STH, basil, cabbage, hookworm, sedimentation
AKTIVITAS INHIBITOR ALFA GLUKOSIDASE DARI AKTINOMISET ASAL PHYLLOPLANE KELOR (Moringa oleifera) Noor Andryan Ilsan; Siti Nurfajriah; Maulin Inggriani
Bioma Vol 14 No 2 (2018): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.257 KB) | DOI: 10.21009/Bioma14(2).1

Abstract

The exploration of alternative natural products is one of the important focus for researcher. Microbes are still the best source for searching the bioactive compounds. Actinomycetes are Gram Positive bacteria which have been known able to produce various of bioactive compounds such as antimicrobials and enzyme inhibitors. The aims of this study were to isolate Actinomycetes from Moringa oleifera phyllosphere also evaluate the antimicrobial and alpha glucosidase inhibitor activities. The result of molecular 16s rRNA gene identification showed FKU 1 isolate had closest related to Nocardia rhamnosiphila. FKU 1 isolate had antimicrobial activities against E.coli, Staphylococcus aureus, Klebsiella pneumonia, K. pneumonia ESBL, dan Vibrio sp. in vitro. FKU 1 also had alpha glucosidase inhibitor activity of 7.50 ± 1.59% at 0.01 µg mL-1 extract concentrations of compared to acarbose (control) of 8.41 ± 0.43%.
PEMERIKSAAN KESEHATAN PADA SAMPEL FESES DAN KEROKAN KULIT MASYARAKAT TPST BANTAR GEBANG BEKASI Intan Kurniawati Pramitaningrum; Maulin Inggraini; Noor Adrya Ilsan; Siti Nurfajriah; Ria Amelia; Elfira Maya Sari
Jurnal Mitra Masyarakat Vol. 1 No. 1 (2019): Jurnal Mitra Masyarakat : Special Edition I
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.742 KB)

Abstract

Daerah Bantar Gebang merupakan tempat pembuangan sampah terbesar di Indonesia. Daerah di sekitar TPST Bantar Gebang memiliki kondisi sanitasi dan sarana air bersih yang kurang. Kondisi lingkungan tersebut sangat potensial dengan masalah kesehatan. Tujuan penelitian ini adalah untuk melakukan pemeriksaan kesehatan pada sampel feses dan kerokan kulit pada siswa di Yayasan Dinamika Indonesia dan Yayasan Tunas Mulia. Metode yang digunakan adalah dengam melakukan pemeriksan natif feses dan pemeriksaan kerokan kulit menggunakan KOH. Dari hasil pemeriksaan 2 dari 61 siswa Yayasan Dinamika Indonesia positif Trichuris trichiura. .Hasil Isolasi bakteri patogen dari feses siswa di Yayasan Dinamika Indonesia dan Yayasan Tunas Mulia, Bantar Gebang Bekasi menunjukkan bahwa sebanyak 16 siswa (26,2 %) terdapat Salmonella sp., 3 siswa (4 %) terdapat Shigella sp. dan 42 siswa (68,8%) terdapat E. coli. Siswa yang diduga terinfeksi jamur kulit di Yayasan Tunas Mulia berjumlah 7 siswa, 6 siswa diantaranya positif dan 1 negatif. Sedangkan pada Yayasan Dinamika Indonesia terdapat 34 siswa yang diduga terinfeksi jamur kulit tetapi hanya 10 siswa yang positif sedangkan 24 siswa negatif
Density of House Dust Mites (HDM) Dermathopagoides sp. In Jatimulya Village South Tambun District Bekasi City Reza Anindita; Salma Lailatul Amwia; Maulin Inggraini; Dede Dwi Nathalia
Jurnal Medis Islam Internasional Vol 3 No 2 (2022): June
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/iimj.v3i2.3015

Abstract

Background: House Dust Mites (HDM) are arthropods that trigger allergies such as asthma and rhinitis. The percentage of these animals as the cause of asthma according to WHO data (2013) is around 50% -80%. Objective: The purpose of this study was to obtain new information about the density of HDM in the city of Bekasi as well as to complement the existing data so that it can be used as a reference in formulating an allergy prevention program caused by HDM. Methods:This  study  was  designedwith  a  quasi-experimental  study  research  method using a comparative pre-test post-test non-equivalent control group design.The sample in this study was divided into two groups, namely one control group and one treatment group  from  the  sample  selected  by  purposive  sampling.  The population studied were students of the Hidayatullah Islamic Boarding School inSurabaya. Result: This type of research is quantitative descriptive with a cross-sectional research design. The sample in this study were 9 houses of residents of the village of Jati Bulak, RT 001/ RW 003, Jatimulya Village, Tambun Selatan District. The location points for dust sampling for each house are mattresses, carpets and floors. The working procedure of this research includes the pre-analytic stage in the form of preparation of tools and materials, the analytical stage in the form of HDM examination with the sedimentation method, the post-analytic stage in the form of confirmation of HDM identification. Conclusion:From research result that has been done, it can be concluded that the HDM figures in 9 houses of Jati Bulak villagers RT 001/ RW 003 Jatimulya Village, Tambun Selatan District are in the low category.
PENCEGAHAN STUNTING DENGAN PENERAPAN PHBS DAN PENGGUNAAN SENYAWA ALAMI : PENCEGAHAN STUNTING DENGAN PENERAPAN PHBS DAN PENGGUNAAN SENYAWA ALAMI Maulin Inggraini; Elfira Maya Sari
Jurnal Mitra Masyarakat Vol. 3 No. 1 (2022): Jurnal Mitra Masyarakat
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Stunting is a chronic malnutrition problemcaused by a lack of nutritional intake for a long time and not caused by hormonal disorders or diseases. Malnutrition in children can cause children to become sick easily, not optimal body postures, intelligence below normal and increase mortility in infants and children. Stunting can result in long-term economic losses for Indonesia. The purpose of this activity is to provide insight to the community to prevent stunting to thrive in children. This activity is carried out online with the target audience of the general public participating in the webinar. To determine of participants education, an evaluation was conducted using pre test and post test questionnaires to participants, then analyzed descriptively. The result of this activity is that the level of community knowledge based on results of the pre test and post test has increased significantly, which is almost 86,42%. This can be seen from the discussion and questions given to the speakers and the highest score obtained. The highest score after the pre test was obtained up to 100 points while the lowest was 15 points. The highest score after the post test is obtained up to 100 points while the lowest is 30 points.
PEMERIKSAAN TELUR SOIL TRANSMITTED HELMINTH (STH) PADA KUBIS (BRASSICA OLERACEA) DAN KEMANGI (OCIMUM BASILICUM) DI PASAR TRADISIONAL BEKASI Reza Anindita; Vincentia Donalda Petra Lerrick; Maulin Inggraini
Meditory : The Journal of Medical Laboratory Vol 10, No 2 (2022): meditory, Volume 10 No 2
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v10i2.2280

Abstract

Worm infections caused by Soil Transmitted Helminth (STH) in Indonesia cause diarrhea, malnutrition, and malaise. One of the epidemiology of STH occurs through soil or fertilizer contaminated with STH in vegetables. Therefore, it is necessary to examine and identify STH in vegetables, especially cabbage and basil. The purpose of this study was to determine STH eggs in vegetables from cabbage (Brassica oleracea) and basil (Ocimum basilicum) taken at Pasar Baru, Bekasi. This type of research is descriptive and quantitative with a cross-sectional design. The samples in this study were 12 cabbage and 9 basil leaves taken from Pasar Baru, Bekasi. The sampling technique used was purposive sampling. Data analysis was done by quantitative descriptive. The results of this study showed that 3 (25%) samples of cabbage and 3 (22.22%) of basil were found to be positive for the presence of STH eggs. The species of STH eggs that were identified were fertile and non-fertile Ascaris lumbricoides. This study concluded that there was contamination of fertile and non-fertile A. lumbrocoides eggs in cabbage and basil vegetables sold in Pasar Baru, Bekasi.Keywords: STH eggs, basil, cabbage, Ascaris lumbricoides, sedimentation method
SKRINNING RHIZOBAKTERI MANGROVE Rhizosphora Sp. PENGHASIL AMILASE Siti Nurfajriah; Maulin Inggraini; Noor Andryan Ilsan
Jurnal Mitra Kesehatan Vol. 1 No. 1 (2018): Jurnal Mitra Kesehatan
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47522/jmk.v1i1.4

Abstract

Pendahuluan: Ekosistem mangrove merupakan ekosistem yang kaya akan nutrisi karena dipengaruhi oleh pasang surut air laut, asupan air tawar dari daratan, akumulasi mineral, dan aktivitas mikroorganisme. Kondisi tersebut menghasilkan ekosistem yang unik dan memiliki keanekaragaman mikroorganisme. Rhizobakteri adalah bakteri yang hidup pada daerah rhizosfer dan membentuk koloni pada sistem perakaran tumbuhan. Rhizobakteri diketahui memiliki bermacam enzim, salah satunya antara lain enzim amilase. Enzim amilase banyak digunakan di industri makanan, tekstil, dam kertas. Metode: Tujuan penelitian ini adalah mengisolasi dan menskrining amilase yang dihasilkan rizhobakteri dari tanaman mangrove dan bakteri serasah pada mangrove Rhizophora Sp. Isolasi bakteri dilakukan dengan seri pengenceran yang ditumbuhkan dalam medium zobell. Skrinning aktivitas amilase dilakukan dengan menumbuhkan bakteri dalam medium zobell agar yang mengandung pati. Hasil: Isolat rhizobakteri yang berhasil diisolasi dari tumbuhan mangrove muda, mangrove tua, dan serasah berjumlah 42 isolat. Hasil skrining menunjukkan 30 isolat mampu menghasilkan a-amilase. Kesimpulan: Berdasarkan penelitian dapat disimpulkan bahwa isolat Rhizobakteri mangrove yang berhasil diisolasi dari akar tanaman mangrove Pulau Bira Kepulauan Seribu sebanyak 42 isolat dan 30 isolat menghasilkan enzim a-amilase. Isolat yang paling banyak menghasilkan enzim tersebut berasal dari rhizobakteri tanaman mangrove muda dan zona bening yang yang terbesar yaitu 7 mm.