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All Journal Journal of Health (JoH) Jurnal Labora Medika Klinikal Sains (Jurnal Analis Kesehatan) Jurnal Farmasi Indonesia Jurnal Biomedika Conference on Innovation in Health, Accounting, and Management Sciences Proceeding

Rinda Binugraheni, Rinda

Program Studi D3 Analis Kesehatan Fakultas Ilmu Kesehatan, Universitas Setia Budi Surakarta

Author-ID : 1614341

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Potensi Antioksidan pada Bubuk Kakao (Theobroma cacao L) Fermentasi Dan Non Fermentasi terhadap Kadar Malondialdehid (MDA) Tikus Putih (Rattus norvegicus Berkenhout, 1769) Hiperlipidemia Rinda Binugraheni; Nastiti Wijayanti
Biomedika Vol 7 No 2 (2014): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Indonesia merupakan negara penghasil kakao terbesar ketiga di dunia. Penelitian-penelitian yang telah dilakukan menunjukan bahwa kakao (Theobroma cacao L) mengandung senyawa polifenol antara lain katekin, epikatekin, proantosianidin, dan antosianin yang berpotensi sebagai senyawa antioksidan. Penelitian ini bertujuan untuk mempelajari potensi antioksidan bubuk kakao (Theobroma cacao L) fermentasi dan non fermentasi terhadap kadar malondialdehid (MDA) tikus putih. Penelitian menggunakan Rancangan Acak Lengkap faktorial dengan delapan perlakuan dan tiga ulangan. Perlakuan terdiri dari kelompok normal, kontrol positif, 3 kelompok uji bubuk kakao fermentasi dan 3 kelompok uji bubuk kakao non fermentasi masing-masing dengan dosis 0,2, 0,4, dan 0,8 gram /200 gram BB. Tikus diberi diet tinggi lemak selama 8 minggu, dan larutan bubuk kakao diberikan 4 minggu terakhir masa penelitian. Pengukuran kadar Malondialdehid (MDA) diukur pada minggu ke-4 dan 8 masa penelitian. Hasil penelitian menunjukan bahwa kandungan total fenol bubuk kakao fermentasi lebih besar dari pada bubuk kakao non fermentasi. Pemberian bubuk kakao fermentasi secara oral mampu menurunkan kadar MDA, sedangkan bubuk kakao non fermentasi belum dapat menurunkan kadar MDA.

Pengaruh Pemberian Bubuk Kakao (Theobroma cacao L) Fermentasi Terhadap Profil Lipid Tikus Putih (Rattus norvegicus Berkenhout, 1769) Hiperlipidemia Rinda Binugraheni; Nastiti Wijayanti
Biomedika Vol 8 No 1 (2015): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Kakao (Theobroma cacao L) merupakan salah satu komoditas perkebunan yang besar di Indonesia. Penelitian-penelitian yang telah dilakukan menunjukan bahwa kakao (Theobroma cacao L) mengandung senyawa polifenol antara lain katekin, epikatekin, proantosianidin, dan antosianin yang berpotensi sebagai senyawa antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian bubuk kakao (Theobroma cacao L) fermentasi terhadap profil lipid tikus hiperlipidemia. Penelitian menggunakan Rancangan Acak Lengkap dengan lima perlakuan dan tiga ulangan. Perlakuan terdiri dari kelompok normal, kontrol positif, 3 kelompok uji bubuk kakao fermentasi masing-masing dengan dosis 0,2, 0,4, dan 0,8 gram /200 gram BB. Tikus diberi diet tinggi lemak selama 8 minggu, dan larutan bubuk kakao diberikan 4 minggu terakhir masa penelitian. Pengukuran uji profil lipid darah dilakukan pada minggu ke-0, 4 dan 8. Hasil penelitian menunjukan bahwa pemberian bubuk kakao fermentasi secara oral mampu menurunkan kadar kolesterol total, trigliserida, LDL, serta meningkatkan kadar HDL.

Pengaruh Ekstrak Etanolik Bekatul Beras Hitam (Oryza sativa L. Cv Woja Laka) terhadap Berat Badan Tikus Putih (Rattus norvegicus Berkenhout, 1769) Diabetik Rahmat Budi Nugroho; Rinda Binugraheni; Rizal Maarif Rukmana
Biomedika Vol 10 No 1 (2017): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Diabetes melitus merupakan suatu penyakit metabolik dengan karakteristik hiperglikemia yang terjadi karena kelainan sekresi insulin, kerja insulin atau keduanya. Beberapa gejala dari penyakit diabet adalah: banyak minum, banyak kencing dan berat badan menurun. Tujuan dari penelitian ini adalah untuk mengetahui konsentrasi ekstrak etanolik bekatul beras hitam (Oryza sativa L. cv Woja Laka) yang paling baik dalam meningkatkan berat badan tikus putih jantan (Rattus norvegicus Berkenhout, 1769) yang menderita diabetik. Manfaat dari penelitian ini adalah memberikan informasi terkait tanaman fungsional yang dapat meningkatkan berat badan pada penderita diabetik. Metode ekstraksi bekatul beras hitam dengan maserasi. Rancangan penelitian dengan Rancangan Acak Lengkap (RAL) dengan enam perlakuan dan empat ulangan. Perlakuan terdiri dari kelompok kontrol normal, kontrol negatif, kontrol positif, 3 kelompok uji ekstrak bekatul beras hitam masing-masing dengan dosis 75 mg/200 g BB, 150 mg/200 g BB dan 225 mg/200 g BB. Semua kelompok perlakuan diinduksi aloksan kecuali kelompok kontrol normal. Ekstrak bekatul diberikan selama 21 hari terakhirmasa penelitian. Hasil penelitian menunjukkan bahwa ekstrak etanolik bekatul beras hitam mampu meningkatkan berat badan tikus putih yang menderita diabet. Kosentrasi yang paling baik untuk meningkatkan berat badan tikus putih jantanyang menderita diabet adalah 225 mg/ 200 g BB.

Deteksi Bakteri Salmonella sp. pada Mie Basah Yang Dijual di Pasar Gede Surakarta Rinda Binugraheni; Rahmat Budi Nugroho
Biomedika Vol 9 No 1 (2016): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Mie basah merupakan makanan yang populer di Indonesia. Makanan yang terkontaminasi oleh mikroorganisme seperti halnya bakteri Salmonella sp, dapat menyebabkan terjadinya keracunan dan penyakit-penyakit bawaan makanan yang dapat mengganggu kesehatan manusia. Penelitian ini menggunakan metode isolasi yang menggunakan media buffer pepton, selenith borth, KIA, SIM, LIA dan Citrat. Sampel yang digunakan adalah mie basah sebanyak 6 jenis yang diambil dari beberapa pedagang mie basah di Pasar Gede Surakarta. Berdasarkan hasil penelitian menunjukan bahwa tidak terdeteksi adanya bakteri Salmonella pada mie basah yang dijual di Pasar Gede Surakarta.

Uji Bakteriologis Susu Kedelai di Mojosongo Surakarta Rahmat Budi Nugroho; Rinda Binugraheni
Biomedika Vol 9 No 1 (2016): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Susu kedelai merupakan produk olahan dari kedelai yang banyak dikonsumsi masyarakat, terutama bagi yang alergi susu sapi karena tidak mampu mencerna laktosa yang terkandung dalam susu sapi. Pengolahan susu kedelai yang tidak baik dapat menyebabkan munculnya bakteri coliform yang dapat menyebabkan gangguan kesehatan. Penelitian ini merupakan penelitian deskriptif yang memberikan gambaran mengenai kualita bakteriologis susu kedelai yang ada di Mojosongo Surakarta, dengan menggunakan tabel Most Probable Number (MPN) dengan ragam 3.3.3. Hasil penelitian menunjukkan bahwa dari lima sampel yang digunakan, 2 sampel tidak mengandung bakteri coliform dengan nilai MPN 0/100 ml sampel. Ketiga sampel lainnya mengandung coliform dengan nilai MPN berturut-turut 93/100 ml sampel, 43/100 ml sampel dan 93/100 m lsampel. Hal ini disebabkan karena bahan baku (air, kedelai) yang digunakan, prosessing dan sanitasinya kurang baik.

Uji Aktivitas Antibakteri Ekstrak Etanolik Kayu Secang (Caesalpinia sappan L.) terhadap Staphylococcus aureus Diah Mukti Cahyaningtyas; Nony Puspawati; Rinda Binugraheni
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Piogenic infection is an infection characterized by the occurrence of severe local inflammation with pus formation (pus). Generally caused by piogenic germs, one of the most common is Staphylococcus aureus. One of the plants used as an ingredient in traditional medicine and used as an anti-bacterial is a secang plant. This study aims to determine the anti-bacterial activity of ethanolic extract of secang wood (Caesalpinia sappan L.) on pure cultivation Staphylococcus aureus Laboratory and isolates pus Hospital patients and find out the difference in inhibition between the two bacterias.300 grams of secang wood powder was extracted by maceration method using 70% ethanol (1:10). Maserate was used to test the anti-bacterial activity of Staphylococcus aureus from pure cultivation laboratory and isolates of pus patients in hospital using diffusion and dilution methods. Then, the results of the diffusion test were analyzed using a two-way Anova test.The results of the study showed that ethanolic extracts of wood have anti-bacterial activity. Result of dilution method showed the value of KBM at a concentration of 3% for Staphylococcus aureus cultivation Laboratory and a concentration of 4% for Staphylococcus aureus isolates pus patients of the Hospital. The statistical analysis of diffusion test results showed that the best concentration in inhibiting the growth of these two bacterias were the concentration of 25% and the sensitivity of Staphylococcus aureus from Laboratory pure cultivation and the Pus patients isolate of the Hospital toward extracts were the same.

Uji Aktivitas Larvasida Ekstrak Etanolik Daun Serai Wangi (Cymbopogon nardus L.) terhadap Larva Culex sp. Instar III Eka Cahya Nugraha; Tri Mulyowati; Rinda Binugraheni
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Culex sp. larvae is a vector of filariasis. The prevention effort is by controlling the presence of Culex sp. Citronella grass is known to contain secondary metabolite which is useful as plant-based insecticides. This research was conducted to determine the toxicity of the ethanolic extract of citronella grass on the mortality of Culex sp. III instar.The research method used was an experimental method with a completely randomized design (CRD) research design, the treatment that was tried was reagent control; negative control; 250 ppm; 353 ppm; 498 ppm; 702 ppm; 991 ppm; 1397 ppm; 1970 ppm, three replications for each treatment. Variables observed were larvae mortality in various concentrations. Larvae mortality was calculated after 24 hours. The data obtained were analyzed using the kruskal wallis test and the man whitney test with SPSS 21 application to determine the LC50value using probit analysis.The results indicate that the ethanolic extract of citronella grass functioned as larvasideonCulex sp. larvae III instar. The higher the concentration of citronella grass extract used, the greater the mortality percentage of Culex sp. larvae. III instar. The concentration of 991 ppm was the most effective concentration and the LC50value was 1036,54 ppm. The conclusion of this research is the ethanolic extract of citronella grass (Cymbopogonnardus L.) has larvicidal activity on Culex sp. larvae. III instar.

Pemeriksaan Kimia Darah (Glukosa Darah, Kolesterol dan Asam Urat) Menggunakan Metode Stick Test dan Metode Spektrofotometri dari Sampel Darah Masyarakat RW 22 Kelurahan Nusukan Kecamatan Banjarsari Kota Surakarta Rinda Binugraheni; Susan Primadevi; Rahmat Budi Nugroho; Dian Kresnadipayana; Gregorius Indra Budianto
Journal of Health (JoH) Vol 3 No 2 (2016): Journal of Health - July 2016
Publisher : LPPM STIKES Guna Bangsa

Nusukan is one village with a tendency for people who prefer to consume fast food and lack of attention to a healthy lifestyle. This causes a lot of society are affected by diabetes mellitus, hyperlipidemia and uric acid. Nusukan society RW 22 who are not aware that they have the disease. For it is necessary to check blood glucose levels, cholesterol and uric acid that can be detected early. The purpose of this program is to provide education on the dangers and prevention of diabetes mellitus, hyperlipidemia, and uric acid. It is also to help people to detect diabetes mellitus, hyperlipidemia and uric acid using stick test and spektropothometry method. Of the 30 people there were seven people who are abnormal blood glucose levels or diabetes mellitus, according stated test stick method, whereas in spectrophotometric methods there are 2 people. Uric acid test of RW 22, there are 7 people who exceed the normal limits, whereas cholesterol levels there are 22 people who exceed normal cholesterol levels (hyperlipidemia) with a test stick method, whereas in sprektropothometry there are 18 people who hyperlipidemia. Methods stick test results show higher levels compared to the spectrophotometric method.

Hubungan Tingkat Pengetahuan, Sikap Serta Tindakan Petani Terhadap Kejadian Infeksi Soil Transmitted Helminths Di Dukuh Ngancan Desa Sobokerto Ngemplak Boyolali Fitin Dwi Mumpuni; Tri Mulyowati; Rinda Binugraheni
Journal of Health (JoH) Vol 7 No 1 (2020): Journal of Health - January 2020
Publisher : LPPM STIKES Guna Bangsa

Worms caused by Soil Transmitted Helminths are a group of intestinal nematode worms that infect through contact with parasitic eggs or larvae that grow on fertile and moist soil. Knowledge about helminthiasis has an important role to prevent helminthiasis, so the tendency of low knowledge will further increase the risk of helminthiasis. The purpose of this study was to determine the relationship of the level of knowledge, attitudes and actions of farmers to the incidence of Soil Transmitted Helminths infection in Dukuh Ngancan Desa Sobokerto Ngemplak Boyolali and the percentage of Soil Transmitted Helminth infections. This type of research uses observational methods with a cross-sectional approach. The sampling technique uses Simple Random Sampling. Data were collected from respondents using a questionnaire and faecal examination with Eosin 2% technique and Sedimentation technique. Data analysis using Chi-Square test analysis. The results showed that there was no correlation between the level of knowledge and actions of farmers against the incidence of Soil Transmitted Helminths infection in Dukuh Ngancan Desa Sobokerto Ngemplak Boyolali, but there was a relationship between farmers' attitudes toward the incidence of parasitic Soil Transmitted Helminths infection in Dukuh Ngancan Desa Sobokerto Ngemplak Boyolali and percentage of Soil Transmitted Transmission in Boyolali. Helminths of 44 respondents who were not infected with Soil Transmitted Helminths 95.5% and those infected with Soil Transmitted Helminths 4.5%.

Uji Aktivitas Antibakteri Ekstrak Etanolik Daun Kecombrang (Nicolaia Speciosa) Terhadap Staphylococcus Aureus Rinda Binugraheni; Ndaru Trisni Larasati
Journal of Health (JoH) Vol 7 No 2 (2020): Journal of Health - July 2020
Publisher : LPPM STIKES Guna Bangsa

Infectious diseases that often occur in humans are skin infections. One of the bacteria that cause infection in humans is Staphylococcus aureus. One of the plants used as an ingredient in traditional medicine and used as an anti-bacterial is a kecombrang plant. This research aims to test the antibacterial activity of leaf extract of kecombrang against Staphylococcus aureus. Kecombrang leaf obtained from Pandeglang Banten. Kecombrang leaves extracted with a maceration method using 96% ethanol solvent. Extracts of leaves are made in a various concentrations (100%, 75%, 50%, and 25%). The antibacterial activity test was performed by using the diffusion method to find out the large zone of diameter are formed to inhibiting Staphylococcus aureus bacteria.The results of the antibacterial activity of the leaves extract of kecombrang to Staphylococcus aureus at 25%, 50%, 75% and 100% concentrations respectively were 12.67 mm, 14.33 mm, 15.33 mm, and 17.00 mm. The data result showed, that leaf extract of kecombrang with 100% concentration had the largest inhibiting zone diameter of 17.00 mm, among other concentrations. This suggests that kecombrang leaf extract have antibacterial activity against Staphylococcus aureus.

Co-Authors Aida Kumara Argo Khoirul Anas Chori Ayu Setyoningrum Diah Mukti Cahyaningtyas Dian Kresnadipayana Eka Cahya Nugraha Elisabet Indita Kusumawardani Erna Setiyani Fitin Dwi Mumpuni Gregorius Indra Budianto Gregorius Indra Budianto, Gregorius Indra Ifandari Ifandari Leona Dwiyan Marahema Muhamad Ansory, Hery Nabilla Tri Oktafiyani Nastiti Wijayanti Ndaru Trisni Larasati Nony Puspawati Panji Pramana Rahmat Budi Nugroho, Rahmat Budi Rizal Maarif Rukmana Susan Primadevi Susan Primadevi, Susan Tiara Khoirunnisaa Tri Mulyowati Tri Mulyowati Tri Mulyowati

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