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All Journal BALI MEDICAL JOURNAL (BMJ) E-Jurnal Medika Udayana ISM (Intisari Sains Medis) : Jurnal Kedokteran Jurnal Respirasi (JR) Neurologico Spinale Medico Chirurgico Journal of Health Sciences and Medicine Essential: Essence of Scientific Medical Journal Indonesian Archives of Biomedical Research (InABR)
Agus Eka Darwinata
Department Of Microbiology, Faculty Of Medicine Udayana Unversity, Denpasar, Bali, Indonesia.
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Journal : Neurologico Spinale Medico Chirurgico

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Topical administration of Tamanu Oil (Calophyllum inophyllum) inhibited the increase of matrix metalloproteinase-1 (MMP-1) expressions and decrease of collagen dermis amount in male wistar rats exposed to ultraviolet B Yoselyn Gunawan; Alex Pangkahila; Agus Eka Darwinata
Neurologico Spinale Medico Chirurgico Vol 4 No 3 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i3.186

Abstract

Background: UV-B irradiation is the major causative factor in photoaging. Photoaging increases MMP-1 significantly and cause damage to collagen by forming ROS. The monounsaturated free fatty acids and antioxidant compounds in Tamanu oil have been studied to inhibit the emergence of MMP-1 and maintain collagen amount. The study aimed to investigate topical Tamanu oil in inhibiting MMP-1 expressions and collagen decrease in male Wistar rats’ skin exposed to UV-B. Methods: A randomized post-test-only control group design was performed on 36 male Wistar rats, aged 2-3 months old with 180-220 grams body weight. They were assigned to two groups: the placebo group and the Tamanu oil group. Both groups were given the same amount of UV-B exposure for four weeks totalling in 840 mJ/cm2 of dosage radiation. Their dermis was examined for MMP-1 using Immunohistochemistry and collagen amount using Picro Sirius Red staining. Results: A comparative analysis was done to show the difference between groups. The mean expression of MMP-1 in the control group was 34,03±2.97 cell/HPF and the Tamanu oil group was lower at 22.73±2.68 cell/HPF. The mean amount of collagen dermis in the control group was 38.90±11.64 % and the Tamanu oil group was higher at 65.66±8.13 %. A significant difference was shown in the two groups (p<0.05). Conclusion: Topical Tamanu oil has proven to hinder the increase of MMP-1 expressions and collagen amount in male Wistar rats’ skin exposed to UV-B.
Co-Authors Agung Wiwiek Indrayani Agus Indra Yudhistira Diva Putra Anak Agung Gde Putra Wiraguna Ananda Eka Raharja F. S. Wignall Hearty Indah Oktavian I Gede Agus Darsana Palgunadi I Gede Bayu Adi Pratama I Gede Gita Sastrawan I Gede Krisna Arim Sadeva I Gede Raka Adhyatma I Gede Sathya Agastya I Gede Wikania Wira Wiguna I Gusti Agung Istri Gladys Elsyaningrat I Gusti Agung Ngurah Dhyana Ratmaja I Gusti Agung Utama Surya Putra I Gusti Ayu Cintya Paramyta I Gusti Ngurah Kade Mahardika I Putu Bintang Pradita Kirana I Wayan Weta I. B. P. Dwija Ida Bagus Gede Adiguna Wibawa Ida Sri Iswari J. A. Pangkahila K. Subrata K. Wirasandhi Komang Januartha Putra Pinatih Komang Januartha Putra Pinatih Ledwin Meikel Wibisono Made Agus Hendrayana Made Indira Dianti Sanjiwani Milhanah Milhanah N. K. Susilarini Ni Made Susilawathi Ni Nengah Dwi Fatmawati Ni Nyoman Sri Budayanti Nyoman Budhi Wirananda Setiawan Putu Agung Satvika Pradnyadevi Putu Nindya Krisnadewi Rahadi Raka-Sudewi A. A. Wimpie I Pangkahila Winnie Chandra Yoselyn Gunawan