Murti, Rudi Hari
Departemen Agronomi, Fakultas Pertanian, Universitas Gadjah Mada, Jalan Flora No.1. Bulaksumur, Sleman, Yogyakarta, 55281

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Korelasi Kandungan Antosianin Total dengan Parameter Warna (L*, a*, dan b*) dan Penanda Mikrosatelit pada Beras Hitam Lokal Kristamtini, Kristamtini; Taryono, Taryono; Basunanda, Panjisakti; Murti, Rudi Hari
Jurnal Penelitian Pertanian Tanaman Pangan Vol 1, No 2 (2017): Agustus 2017
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.063 KB) | DOI: 10.21082/jpptp.v1n2.2017.p115-124

Abstract

Black rice is a functional food for its  high anthocyanin contents expressed on purple-black rice color. The study aimed to determine the correlation between: total anthocyanin content with  L *, a *, and b * color variable, total anthocyanin content with color morphology and total anthocyanin content microsatellite markers. Eleven local black rice varieties  from Yogyakarta Assesment Institute of Agriculture Technology collections and two varieties of white rice (Situbagendit and Inpari 6 Jete) were planted on plastic pots in a green house. The tried was arranged   completely randomized design with five replications. At three weeks after planting, the leaf samples were are taken for DNA isolation and further PCR amplifications  using four microsatellite markers (RM 180, RM 220, RM 224 and RM 252). The black rice grain were observed based on color morphology using a Chromameter and analyzed  for then anthocyanin contents. Regression, correlation, and path analysis were conducted to determine the relationship between total anthocyanin content and L* a* b * color variable, color morphology, and microsatellite markers. The results showed that there is correlation between total anthocyanin content  with color parameters (L* = -0,568,  a* = -0,561, b*= 0,844).  Total anthocyanin content is correlation too with color scor of morfology and total   microsatellite markers ((RM 180 =-0,419, RM 220 =0,27, RM 224 =-0,493, RM 252 =0,265) and color scor of morphology  = -0,442. Selection of high-anthocyanin content of rice can use the color variable criterion (L*an  a* low, and  b * high). Microsatellite markers RM 220 and RM 252 can be used as moleculer markers for rice of high anthocyanin content.
Pengaruh Tomat Sambung Pada Intensitas Penyakit Layu Bakteri (Ralstonia solanacearum), Komponen Hasil Produksi, dan Kualitas Buah Lisa Navitasari; Tri Joko; Rudi Hari Murti; Triwidodo Arwiyanto
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 3 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.3.413

Abstract

Ralstonia solanacearum (Smith) is one of soil borne pathogens causes bacterial wilt diseases and R. solanacearum is difficult to control because it has a long survival in the soil and have many hosts alternatives. One alternative to control R. solanacearum and to increase productivity is by using grating with combination of resistant varieties of tomato as a rootstock and high production varieties of tomato as a scion. Several studies on grafting to suppress R. solanacearum were reported. However, study on grafting with combination between resistant tomato varieties (Amelia H7996) and high-production tomato varieties with R. solanacearum infestation and without R. solanacearum infestation on the component of yield and fruit quality is limited. The study aims to analyze the effect of grafting with R. solanacearum infestation to the intensity of bacterial wilt disease, component of yield, and fruit quality with R. solanacearum infestation and without R. solanacearum infestation. The result indicated that the intensity of bacterial wilt disease on grafted tomato did not significantly different from resistant rootstock but significantly different from scion. Infestation of R. solanacearum on grafted tomato can decreased the plant productivity that decreased the component of yield on grafted tomato. R. solanacearum infestation also decreased the fruit quality on diameters and fruit firmness. Nevertheless, R. solanacearum did not affect the Total soluble solid/TSS (°Brix). TSS on grafted tomato indicated that the value is taller than rootstock in the field with R. solanacearum infestation and in the field without R. solanacearum infestation. Keywords: fruit quality, productivity, Rasltonia solanacearum, tomato grafted
Komponen Epidemi Penyakit Busuk Akar dan Pangkal Batang Tebu di Sumatera Selatan Tri Maryono; Ani Widiastuti; Rudi Hari Murti; Achmadi Priyatmojo
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.49-60

Abstract

Epidemic Components of Sugarcane Root and Basal Stem Rot In South Sumatra Root and basal stem rot disease is one of new disease that currently was became a emerging problem on sugarcane plantations in Lampung and South Sumatra. The research was aimed to study the model of disease progression, AUDPC, infection rate (r), and the influence of weather and soil condition (physical and chemical properties of soil) on the development of sugarcane root and basal stem rot disease. The research was conducted on sugar cane plantation in South Sumatra. The disease incidence in three varieties was observed weekly in the field which had infected plant in previous year.. The data were analyzed to develop model of the disease progression, AUDPC, and the rate of root and basal stem rot disease. Weekly weather data was acquired from weather station of Sultan Mahmud Badaruddin Airport, Palembang. Soil sample was taken from three disease category i.e moderate (disease incidence 25,1% - 50%), severe (disease incidence 50,1% - 75%), and healthy (no disease). The results showed that the disease develops following monomolecular and logistic models. The AUPDC and infection rate (r) in the monomolecular model were lower than in the logistic model. The direct effect coefficient of rainfall and relative humidity (RH) on the disease were positive (1.27 and 0.46 respectively), contrary the temperature and duration of irradiation which had a negative coefficient (-0.33 and -0.45 respectively). Meanwhile, K availability and permeability give a positive effect on the disease, while the total N and Fe availability give a negative effect on the disease.
BERAS HITAM SUMBER ANTOSIANIN DAN PROSPEKNYA SEBAGAI PANGAN FUNGSIONAL Kristamtini Kristamtini; Taryono Taryono; Panjisakti Basunanda; Rudi Hari Murti
Jurnal Penelitian dan Pengembangan Pertanian Vol 33, No 1 (2014): Maret 2014
Publisher : Badan Penelitian dan Pengembangan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jp3.v33n1.2014.p17-24

Abstract

Beras hitam merupakan sumber antosianin yang tinggi dan murah dibandingkan dengan sumber antosianin lain seperti bluberi dan anggur. Antosianin merupakan senyawa organik golongan flavonoid dengan struktur utama tiga gugus aromatik. Sebagai antioksidan, antosianin bermanfaat bagi kesehatan, di antaranya untuk mencegah penuaan dini, melindungi lambung dari kerusakan, menghambat sel tumor, sebagai senyawa antiinflamasi dan antikanker, melindungi otak dari kerusakan, mencegah obesitas dan diabetes, meningkatkan kemampuan memori otak, mencegah penyakit neurologis, dan menangkal radikal bebas dalam tubuh. Beras hitam juga mengan-dung protein, vitamin, dan mineral yang lebih tinggi daripada beras putih. Berdasarkan hal tersebut, diperlukan penjelasan tentang struktur kimia antosianin, sifat antosianin dan manfaatnya bagi kesehatan dan aplikasi industri, beras hitam sebagai sumber antosianin, distribusi dan sintesis antosianin, manfaat antosianin bagi tanaman, dan prospek pengembangan beras hitam di Indonesia.