Claim Missing Document
Check
Articles

Found 11 Documents
Search

KloningOpen Reading Frame (orf) ESAT-6 (Early Secretory Antigenic Target-6) Mycobacterium tuberculosis ke Escherichia coliBL21 (DE3) Agus, Rosana; Maidin, Asaad; Hatta, Mochammad; S.Retnoningrum, Debbie
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.365 KB) | DOI: 10.33476/jky.v16i3.245

Abstract

Tuberculosis (TB) caused by Mycobacterium tuberculosis is an infectious diseases leading to significant death toll in most partof the world. Vaccination with BCG, a TB vaccine, is still a common practice until now. In general, the people in Indonesia receive BCG vaccine during their early childhood, but the efficacy of the vaccine would not last long to adulthood, which allow themto get potential latent TB infection. This latent TB infection might be detected by tuberculin skin test (TST), however, the weakness is that false positive reactions are commonly found due to cross reaction between antibodies produced during BCG vaccination and purified protein derivative (PPD). Alternatively, its detection could be performed by identifying immunodominant antigen to M. tuberculosis. The Early Secretory AntigenicTarget-6 (ESAT-6) for antibody based serological detection with high sensitivity and specificity could also be applied. The purpose of this study was to clone the open reading frame (orf) of ESAT-6 from Mycobacterium tuberculosis into Escherichia coli BL21 (DE3). In this method, orf ESAT-6 was ligated to the expression vector pET-32b and transformed into E. coli BL21 (DE3). Characterization of clones was carried out by cutting the recombinant plasmid using restriction enzymes BamH1 and XhoI. The result showed that three colonies with recombinant plasmid pET-32b-ESAT-6 were obtained. Sequencing of the DNA insert was then performed using the universal T7 primer. Characterization of white colonies with restriction enzymes showed two bands i.e. 288 bp and 5900 bp for orf ESAT-6 and pET32b vector respectively. BLAST analysis of sequence showed 100% homology.
PENGARUH KONSENTRASI CELITE (DIATOM) TERHADAP HASIL ISOLASI DNA BAKTERI Escherichia coli Genisa, Marlina Ummas; Agus, Rosana; Hatta, Mohc
Maspari Journal : Marine Science Research Vol 7, No 2 (2015): Edisi Juli
Publisher : UNIVERSITAS SRIWIJAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (839.639 KB) | DOI: 10.36706/maspari.v7i2.2452

Abstract

Penelitian ini bertujuan untuk menentukan konsentrasi celite (diatom) yang tepat dalam isolasi DNA bakteri Escherichia coli, sehingga diperoleh DNA Genom dengan konsentrasi tinggi. Metode yang digunakan yaitu metode Boom. Dalam penelitian ini menggunakan tiga variasi konsentrasi celite, yaitu 10 µl, 20 µl, 30 µl dan kontrol tanpa celite. Hasil penelitian menunjukkan bahwa secara kualitas (hasil elektroforesis) konsentrasi celite 20 µl dan 30 µl memperlihatkan pita DNA yang leibh tebal dan jelas. Nilai konsentrasi DNAbakteri Escherichia coli dari konsentrasi celite 10 µl, 20 µl, dan 30 µl berturut-turut adalah 0,032 µg/µl, 0,075 µg/µl, dan 0,042 µg/µl. Kontrol tanpa celite 0,009 µg/µl. Angka tersebut menunjukkan bahwa pemberian konsentrasi celite 20 µl pada sampel memiliki konsentrasi DNA yang paling tinggi (0,075 µg/µl) dibanding dengan konsentrasi lain dan kontrol tanpa celite.KATA KUNCI: Escherichia coli, isolasi DNA genom, konsentrasi celite.
Biodiversity of Marine Tunicates at Samalona Waters, Sangkarang Archipelago, Indonesia Magdalena Litaay; Slamet Santosa; Eva Johannes; Rosana Agus; Willem Moka; Jennyta Dhewi Darmansyah Tanjung
Jurnal Ilmu Kelautan SPERMONDE VOLUME 4 NOMOR 1, 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v4i1.3616

Abstract

The study aims to know the biodiversity and community structure of marine tunicate of Samalona waters. The present study is part of biodiversity assessment for marine resources of Sangkarang Archipelago SW Makassar Indonesia. Field campaign was conducted from October to November 2016. Sample collection was done at depth of 3 m and 5 m by using Line Intersection Transect (LIT) method combination plot. Two transects size of 50 m were placed parallel to a shore line at three stations (sta.) at Samalona waters. Plot size 2.5 x 2.5 m was placed side by side of transect and all tunicate inside plot was noted, identified, counted and photographed. Sample collection was collected by using SCUBA and under water camera. Environmental parameters including water temperatue, salinity, dissolved oxygen, clarity, current speed, and wind speed were measured in situ. Data were analysed using ecological indices including species composition and density, Shanon Wienner species diversity, Evenness, and Morisita Indices. The result indicates that there are 18 species of tunicates present at depth of 3 m and 7 meters of Samalona waters, respectively. The result of the ecological analysis shows that species diversity is categorized moderate and no dominant species. Environment parameters indicatess that water quality at Samalona waters is in good condition to support tunicates.
BIODIVERSITY OF MARINE TUNICATES IN SAMALONA WATERS, SANGKARANG ARCHIPELAGO, INDONESIA Magdalena Litaay; Slamet Santosa; Eva Johannes; Rosana Agus; Willem Moka; Jennyta Dhewi Darmansyah Tanjung
Jurnal Ilmu Kelautan SPERMONDE VOLUME 4 NOMOR 1, 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v4i1.3920

Abstract

The study aims to know the biodiversity and community structure of marine tunicate in Samalona waters. The present study is part of biodiversity assessment for marine resources of Sangkarang Archipelago SW Makassar Indonesia. Field campaign was conducted from October to November 2016. Sample collection was done at 3 and 7 m depth by using Line Intersection Transect (LIT) method combined with a quadrat (plot). Two 50 m transects were placed parallel to a shore line at three stations (sta.) at Samalona waters. A quadrat (plot) (2.5 m x 2.5 m) was placed side by side of the line transect and all tunicates in the transect was recorded, identified, counted and photographed. Samples were collected by using SCUBA and under water camera. Environmental parameters including water temperatue, salinity, dissolved oxygen, clarity, current and wind speed, were measured in situ. Data were analysed using ecological indices including species composition and density, Shanon Wienner species diversity, Evenness, and Morisita Indices. The result indicates that there are 18 species of tunicates present at 3 m as well as 7 m depth of Samalona waters.. Result of the ecological analysis shows that species diversity can be categorized as moderate and there were no dominant species. Environmental parameters indicates that water quality at Samalona waters was in good condition to support tunicates.
Deteksi Methicillin Resistant Staphylococcus aureus (MRSA) Pada Pasien Rumah Sakit Universitas Hasanuddin Dengan Metode Kultur Nismawati Nismawati; Rizalinda Sjahril; Rosana Agus
Prosiding Seminar Biologi Vol 4 No 1 (2018): Prosiding Seminar Nasional Megabiodiversitas Indonesia
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v4i1.5932

Abstract

Methicillin-Resistant Staphylococcus aureus (MRSA) resisten terhadap beberapa kelas antibiotik, sehingga merupakan agen penting dari infeksi nosokomial yang sering dikaitkan dengan peningkatan mortalitas dan biaya kesehatan yang tinggi. Penelitian ini bertujuan untuk melakukan deteksi dini pada pasien di Instalasi Gawat Darurat (IGD) sebagai salah satu upaya pengendalian infeksi untuk mencegah penyebaran MRSA di lingkungan rumah sakit. Penelitian ini merupakan penelitian deskriptik analitik dengan teknik Accidental sampling. Pengambilan sampel dilaksanakan di IGD RS Universitas Hasanuddin dan pengamatan dilaksanakan di laboratorium Mikrobiologi RS Universitas Hasanuddin Makassar. Sampel yang diperoleh dari 68 pasien ditanam pada media Nutrient Agar (NA) yang selanjutnya dilakukan pewarnaan gram dan uji biokoimia dengan menggunakan media Manitol Salt Agar (MSA) dan uji koagulase, setelah ditemukan isolat Staphylococcus aureus selanjutnya dilakukan uji sensitivitas terhadap antibiotik cefoxitin 30 µg. Hasil penelitian ini menunjukkan bahwa ditemukan Methicillin Resistant Staphylococcus aureus (MRSA) pada pasien IGD Rumah Sakit Universitas Hasanuddin. Dari 68 sampel diperoleh 13 sampel  (19,1%) membawa Staphylococcu aureus, yang terdiri dari  4 sampel (5,9%) positir MRSA, dan 9 sampel (13,2%) senstif terhadap antibiotik cefoxitin 30 µg.
PENGARUH KONSENTRASI CELITE (DIATOM) TERHADAP HASIL ISOLASI DNA BAKTERI Escherichia coli Marlina Ummas Genisa; Rosana Agus; Mohc Hatta
Maspari Journal : Marine Science Research Vol 7, No 2 (2015): Edisi Juli
Publisher : UNIVERSITAS SRIWIJAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (839.639 KB) | DOI: 10.36706/maspari.v7i2.2452

Abstract

Penelitian ini bertujuan untuk menentukan konsentrasi celite (diatom) yang tepat dalam isolasi DNA bakteri Escherichia coli, sehingga diperoleh DNA Genom dengan konsentrasi tinggi. Metode yang digunakan yaitu metode Boom. Dalam penelitian ini menggunakan tiga variasi konsentrasi celite, yaitu 10 µl, 20 µl, 30 µl dan kontrol tanpa celite. Hasil penelitian menunjukkan bahwa secara kualitas (hasil elektroforesis) konsentrasi celite 20 µl dan 30 µl memperlihatkan pita DNA yang leibh tebal dan jelas. Nilai konsentrasi DNAbakteri Escherichia coli dari konsentrasi celite 10 µl, 20 µl, dan 30 µl berturut-turut adalah 0,032 µg/µl, 0,075 µg/µl, dan 0,042 µg/µl. Kontrol tanpa celite 0,009 µg/µl. Angka tersebut menunjukkan bahwa pemberian konsentrasi celite 20 µl pada sampel memiliki konsentrasi DNA yang paling tinggi (0,075 µg/µl) dibanding dengan konsentrasi lain dan kontrol tanpa celite.KATA KUNCI: Escherichia coli, isolasi DNA genom, konsentrasi celite.
PROFIL DNA GEN FOLLICLE STIMULATING HORMONE RESEPTOR (FSHR) PADA WANITA AKNE DENGAN TEKNIK PCR DAN SEKUENSING DNA Sjafaraenan Sjafaraenan; Handayani Lolodatu; Eva Johannes; Rosana Agus; Arfan Sabran
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 3 No. 1 (2018)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v3i1.3909

Abstract

Penelitian tentang Profil DNA Gen Follicle Stimulating Hormone reseptor (FSHr) pada Wanita Akne dengan Teknik PCR dan Sekuensing DNA. Penelitian ini bertujuan untuk mengetahui profil DNA Gen Follicle Stimulating Hormone reseptor (FSHr) pada Wanita Akne dengan Teknik PCR dan Sekuensing DNA. Penelitian ini dilakukan pada bulan Februari-April 2016. Metode penelitian ini adalah observasional dengan rancangan penelitian cross sectional, teknik laboratorium dilakukan sesuai dengan prosedur PCR dan Sekuensing DNA. Pengambilan sampel dilakukan di Instalasi Laboratorium RS. Labuang Baji Makassar dan Analisis sampel dilakukan di Laboratorium Genetika dan Laboratorium Terpadu Gedung SCIENCE BUILDING Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Hasanuddin, Makassar, Sulawesi Selatan. Pengambilan serum darah melalui vena mediana kubiti dilakukan pada 10 responden wanita penderita akne, yang bersedia menjadi responden dan telah memenuhi kriteria inklusi. Hasil penelitian menunjukkan, bahwa setiap responden memiliki gen FSHr, 4 sampel yang di sekuensing memiliki genotipe Asn385Asn, Asn385Ser, dan Ser385Ser. Kesimpulan dari penelitian diketahui bahwa Profil DNA Gen Follicle Stimulating Hormone reseptor (FSHr) pada wanita akne yaitu Asn385Asn, Asn385Ser, dan Ser385Ser dimana polimorfisme yang terjadi pada Gen FSHr dapat menyebabkan terjadinya gangguan signal tranduksi pada sel granulosa ovarium.Kata kunci : Gen FSHr, Akne, PCR, Sekuensing DNA
ASPEK GENETIKA WARAN KULIT KELOMPOK KOMUNITAS TO BALO DI SULAWESI SELATAN Andi Arfan Sabran; Rosana Agus; Mochammad Hatta
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 3 No. 1 (2018)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v3i1.5561

Abstract

To Balo adalah sebutan untuk satu komunitas masyarakat di Sulawesi Selatan yang memiliki kondisi hipopigmentasi khas pada kulitnya. Hipopigmentasi diduga piebaldism menimbulkan stereotype negatif pada kelompok masyarakat tersebut. Tujuan penelitian ini adalah untuk analisa pedigree guna melihat aspek genetika karakter warna kulit pada komunitas To Balo. Dari hasi analisa pedigree ditemukan bahwa pola pewarisan sifat hipopigmentasi pada masyarakat To Balo adalah dominan autosom. Hal ini serupa dengan pola pewarisan sifat pada piebaldism.Kata Kunci : To Balo, piebaldism, analisis pedigree
Ligation, Transformation and Characterization of Rv 1926c Mycobacterium tuberculosis to Escherichia coli JM 109 For Latent Tuberculosis Immunodiagnostic Rosana Agus
International Journal of Applied Biology Vol. 2 No. 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i2.5813

Abstract

Tuberculosis caused by Mycobacterium tuberculosis is the biggest infectious disease causing human death in the world. The main challenge in controlling tuberculosis is to quickly and accurately diagnose tuberculosis infection. Several kits have been produced to diagnose tuberculosis, but have different sensitivity and specificity. This shows that the kit is not yet ideal for diagnosing tuberculosis, so the search for candidates for specific antigens still needs to be done. One potential antigen is the Rv 1926c encoding MPT 63 protein. This protein is known to induce Th1 cells and produce IFN λ from PBMC cells of patients infected with tuberculosis. The purpose of this study was to clone the Rv 1926c from Mycobacterium tuberculosis as a tuberculosis immunodiagnostic kit. The method used is isolating Rv 1926c with PCR, ligation to pGEM-T vector and transformation to E.coli host cell JM 109. Clone characterization was carried out by PCR and migration analysis. The results obtained are the recombinant clones obtained have successfully inserted with the Rv 1926 c
Penambahan Spirulina Pada Poto Poto Untuk Meningkatkan Kualitas Produk Pangan di Kab. Sinjai Zaraswati Dwyana; Nur Haedar Nur Haedar; Moh. Ivan Azis; Rosana Agus; Muh. Ruslan Umar
Jurnal Ilmu Alam dan Lingkungan Vol. 10 No. 2 (2019): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.017 KB) | DOI: 10.20956/jal.v10i2.7658

Abstract

Spirulina is a marine phytoplankton that is a microalgae that has great potential in life, one of which is food, because the nutritional coverage contained in this marine phytoplankton includes proteins, carbohydrates, vitamins, minerals, essential fatty acids DHA and EPA so that it can be used as an alternative nutritious food. . The purpose of this activity is to training on inovative snack “Poto Poto Sinjai”, how to make Spirulina in Bongki Village, Sinjai Regency. The method used in this activity consisted of a location survey and the production of snack from Spirulina . The implementation phase of the activity using counseling methods and training on the benefits of Spirulina as nutritious food and making snack Spirulina. The results of the implementation of this activity were the increase in participants' knowledge about snack Spirulina, as a highly nutritious snack. The results of the knowledge improvement test show that there is a fairly good increase in knowledge of the snack Spirulina innovation products and the results of the organoleptic test show that preference for taste, aroma, texture and color shows good results.