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Journal : Zuriat

Evaluasi Ukuran dan Bentuk Biji Genotip-genotip Mutan Kedelai Generasi M4 Elfan Waisimon; Noladhi Wicaksana; Anas Anas; Meddy Rachmadi
Zuriat Vol 30, No 1 (2019): Latest Issue (April 2019)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.077 KB) | DOI: 10.24198/zuriat.v30i1.22935

Abstract

Soybean was one of the most important crop plants in Indonesia, but the need has not been fulfilled yet because its productivity was low. This study aims to obtain genotypes of M4 soybean mutant generation based on phenotypic characters of seed size and seed shape. The experiment was carried out in the experimental methods without spatial design, used 19 soybean mutant genotypes on M4 generation and Arjasari variety as a control. Eight characters of seed size (seed length, seed thickness, seed width, seed dimension, and 100 seed weight) and seed shape (seed length–thickness ratio, seed length-width ratio, and seed thickness–width ratio) have observed. Data analyzed with F-test, z-test, cluster analysis, and correlation analysis. The results showed that the seed size and seed shape characters had narrow intrapopulation variations. Eight genotypes of soybean mutants, namely MRAR 14-11-17-5, MRAR 15-17-31-11, MRAR 26-6-21-8, MRAR 29-12-26-11, MRAR 29-15-33 -7, MRAR 29-15-36-4, MRAR 29-15-36-8, and MRAR 29-15-30-10, have more significant seed size characters compared to Arjasari varieties. The MRAR genotypes 15-17-31-11 and MRAR 29-12-27-8 have a more rounded seed shape than the Arjasari variety. The seed size and seed shape characters correlated very strongly with each other. Seed size characters do not associate with seed shape characters.
Keberhasilan Persilangan Tomat Varietas Komersial (Lycopersicum esculentum L.) dengan Tomat Mutan Tahan Simpan Gungun Wiguna; Elfira Rosalita; Anas Anas; Neni Rostini; Syariful Mubarok; Hiroshi Ezura
Zuriat Vol 30, No 1 (2019): Latest Issue (April 2019)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.692 KB) | DOI: 10.24198/zuriat.v30i1.23205

Abstract

Post-harvest loss is an obstacle for tomato farmers that cause tomato farming being unprofitable. Utilization of mutant genes prolong tomato fruit shelf life through crossing is one of the best solutions to improve the fruit quality. The objective of this study was to determine the success of cross-pollination between commercial tomato varieties and shelf life of mutant tomatoes. Two mutant tomato lines Sletr1-1 and Sletr1-2, and one wild type strain were crossed with four commercial varieties (Intan, Mutiara, Ratna, and Mirah ). The results showed that the success rate of crossing ranged from 77.50% to 100%, with an average of 95.76%. The average maximum growth potential of seeds produced was 89.17% with the lowest value of 75% and the highest 98.5%. The most top success of crosses was built by a combination of crossing Ratna x Sletr1-2, and the combination of Mirah x Sletr1-1 showed the lowest percentage of the crossing. The Intan showed the highest success as female parent of crossing, while Mirah was the lowest production of the crossing. The plant height of all hybrid was smaller compared to the commercial varieties.
Pemanfaatan Marka Rapd Untuk Mengestimasi Diversitas Genetik Progeni Half-Sib Paraserianthes falcataria (L.) Nielsen Hasil Seleksi Ketahanan Boktor Dan Riap Pertumbuhan Neni Rostini; S. Sunarya; Murdaningsih H. K.; Anas Anas; F. Damayanti
Zuriat Vol 29, No 2 (2018): Zuriat Vol. 29 No. 2 (Desember 2018)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.651 KB) | DOI: 10.24198/zuriat.v29i2.15835

Abstract

Diversitas genetic pada progeni half sib dari  Paraserianthes falcataria diperlukan untuk mendukung program pemuliaan pada albasia. Tujuan penelitian  untuk mengestimasi diversitas genetik berdasarkan Random Amplified Polymorphic DNA (RAPD) dari  progeni half sib P. falcataria pada empat provenan yang berbeda, yaitu : Banjarnegara, Subang, Kediri dan Kuningan dari albasia yang telah diseleksi tahan terhadap boktor dan riap pertumbuhan.  Sampel daun diambil dari 160 kecambah yang dikoleksi dari 16 tetua dengan empat tetua dari masing masing provenan.  RAPD menggunakan 30 praimer polimorfik. Hasil penelitian menunjukkan diversitas genetik berdasarkan Random Amplified Polymorphic DNA (RAPD) dari  progeni half sib P. falcataria adalah PP/L=1.82, PPL=77.50% dan He=0.240.  Jarak genetik antar populasi berkisar antara 0.025 sampai 0.140 berdasarkan Gregorius (1974), dan antara 0.094 sampai 0.266 berdasarkan Nei (1972).  Beberapa progeni half sib dari tetua yang sama berada pada kluster yang sama. Pengklusteran provenan tidak berhubungan dengan distribusi berdasarkan geografi. Kekerabatan genetik di antara progeni half sib terbagi menjadi tiga grup berdasarkan Nei (1972). Progeny dari Kediri dan grup ketiga dari Kuningan.