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All Journal BERKALA FISIKA SAINS DAN MATEMATIKA YOUNGSTER PHYSICS JOURNAL Jurnal Pendidikan Fisika Indonesia Journal of Physics and Its Applications
Muchammad Azam, Muchammad
Departemen Fisika, Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang
Astronomy Earth & Planetary Sciences Education Materials Science & Nanotechnology Mathematics Physics

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DESIGN OF ELECTROPHORESIS DEVICE FOR OPTIMATION OF DNA VISUALIZATION AND DNA CONCENTRATION USING SOFTWARE Kusumaningrum, H.P.; Budi, W. S.; Azam, M.; Bawono, A.
Jurnal Pendidikan Fisika Indonesia Vol 10, No 2 (2014): July 2014
Publisher : Physics Department, Faculty of Mathematics and Natural Sciences, Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpfi.v10i2.3456

Abstract

Molekul DNA menunjukkan polarisasi yang kuat sehingga memungkinkan baik gerak elektroforesis berdasarkan muatan negatifnya maupun gerak dielektroforesis berdasarkan induksi polarisasi. Perancangan alat menggunakan kombinasi prinsip elektroforesis dan dielektroforesis dilengkapi perangkat lunak untuk mengukur konsentrasinya sangat diperlukan. Utamanya mengingat uji kualitatif DNA berbasis visualisasi pada gel elektroforesis bersifat sangat subyektif dan kurang terukur. Pengukuran konsentrasi DNA menggunakan spektrofotometer UV/VIS sangat tergantung oleh ketersediaannya di laboratorium. Penelitian bertujuan untuk mendesain piranti untuk mengukur konsentrasi DNA berdasarkan visualisasinya pada gel elektroforesis menggunakan perangkat lunak berbasis MatLab. Pengukuran konsentrasi DNA didasarkan visualisasinya pada gel elektroforesis lalu dibandingkan dengan hasil penghitungan spektrofotometer UV/VIS. Hasil penelitian menggunakan piranti tersebut memperlihatkan visualisasi DNA yang lebih optimal. Hasil pengukuran jumlah DNA menggunakan spektrofotometer memiliki kecenderungan yang sama dengan hasil pengukuran menggunakan perangkat lunak berbasis MatLab meskipun terdapat perbedaan nilai kuantitatif.ABSTRACTMolecules of deoxyribo nucleic acid (DNA) show a strong polarization allowing for both motions of the dielectrophoresis induced by polarization and electrophoresis based on its negative charge. Considering high subjective and less quantifiable result of the visualization based qualitative test of DNA on gel electrophoresis, designing the tool using a combination of the principles of electrophoresis and dielectrophoresis completed with a software for optimization of DNA visualization and to measure the concentration of small and large–sized DNA fragment is very needed. Accuracy of measurement of DNA concentration using a spectrophotometer UV /VIS is depend on its availability in the laboratory. The aim of this study was to design device for optimization of DNA visualization and measuring the concentration in the gel electrophoresis using MatLab- based software. Experiment using this software measured the concentration of DNA based on its visualization and compared it with calculation obtained from spectrophotometer UV/VIS. The research results showed that the amount of DNA analysed using a spectrophotometer tend to similar with the measurement results using the MatLab-based software although there was differences in quantitative values.
DESIGN OF ELECTROPHORESIS DEVICE FOR OPTIMATION OF DNA VISUALIZATION AND DNA CONCENTRATION USING SOFTWARE Kusumaningrum, H.P.; Budi, W. S.; Azam, M.; Bawono, A.
Jurnal Pendidikan Fisika Indonesia Vol 10, No 2 (2014)
Publisher : Department of Physics, Faculty of Mathematics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpfi.v10i2.3456

Abstract

Molekul DNA menunjukkan polarisasi yang kuat sehingga memungkinkan baik gerak elektroforesis berdasarkan muatan negatifnya maupun gerak dielektroforesis berdasarkan induksi polarisasi. Perancangan alat menggunakan kombinasi prinsip elektroforesis dan dielektroforesis dilengkapi perangkat lunak untuk mengukur konsentrasinya sangat diperlukan. Utamanya mengingat uji kualitatif DNA berbasis visualisasi pada gel elektroforesis bersifat sangat subyektif dan kurang terukur. Pengukuran konsentrasi DNA menggunakan spektrofotometer UV/VIS sangat tergantung oleh ketersediaannya di laboratorium. Penelitian bertujuan untuk mendesain piranti untuk mengukur konsentrasi DNA berdasarkan visualisasinya pada gel elektroforesis menggunakan perangkat lunak berbasis MatLab. Pengukuran konsentrasi DNA didasarkan visualisasinya pada gel elektroforesis lalu dibandingkan dengan hasil penghitungan spektrofotometer UV/VIS. Hasil penelitian menggunakan piranti tersebut memperlihatkan visualisasi DNA yang lebih optimal. Hasil pengukuran jumlah DNA menggunakan spektrofotometer memiliki kecenderungan yang sama dengan hasil pengukuran menggunakan perangkat lunak berbasis MatLab meskipun terdapat perbedaan nilai kuantitatif.ABSTRACTMolecules of deoxyribo nucleic acid (DNA) show a strong polarization allowing for both motions of the dielectrophoresis induced by polarization and electrophoresis based on its negative charge. Considering high subjective and less quantifiable result of the visualization based qualitative test of DNA on gel electrophoresis, designing the tool using a combination of the principles of electrophoresis and dielectrophoresis completed with a software for optimization of DNA visualization and to measure the concentration of small and large–sized DNA fragment is very needed. Accuracy of measurement of DNA concentration using a spectrophotometer UV /VIS is depend on its availability in the laboratory. The aim of this study was to design device for optimization of DNA visualization and measuring the concentration in the gel electrophoresis using MatLab- based software. Experiment using this software measured the concentration of DNA based on its visualization and compared it with calculation obtained from spectrophotometer UV/VIS. The research results showed that the amount of DNA analysed using a spectrophotometer tend to similar with the measurement results using the MatLab-based software although there was differences in quantitative values.
KAJIAN PENGARUH WARNA DAN JARAK LAMPU PENGAMAN TERHADAP HASIL RADIOGRAF Setiyono, Setiyono; Azam, M.; Setiawati, Evi
BERKALA FISIKA Vol 12, No 1 (2009): Berkala Fisika
Publisher : BERKALA FISIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.477 KB)

Abstract

The study of influence of distance, time and color for safety lamp filter variation to radiograph has been done.  The research used blue sensitive film and green sensitive, type ­-X ray, film. The research was conducted by illuminating film with safety lamp red, yellow, green and blue color filter. The type of filter was red acrylic riddle and glass which was layered in red, yellow, green and blue scot light. The variation of irradiating distance between safety lamp with film was 20 cm, 30 cm, 40 cm, 50 cm, 60 cm, 70 cm, 80 cm, 90 cm, 100 cm and variation of time is 10 second, 15 second, 20 second, 25 second, 30 second, 35 second, 40 second, 45 second, 50 second, 55 second, and 60 second. Respectualy measurement of irradiating resulted used measuring densitometer instrument. The result of research give an illustration that variation of distance, variation of time and color of safety lamp filter influence the radiograph.  The greater distance caused the fog result becomes smaller.  The longer time caused the fog result becomes bigger.  To red acrylic filter, the fog that resulted exceed the tolerance limit is more than 0,2, but for glass filter that layered red scot light is not more than tolerance limit. Keyword: Radiograph, Blue sensitive film, Green sensitive film, Fog, Scot light.
Analisis Fenomena Elektrohidrodinamik pada Permukaan Minyak Silikon Menggunakan Plasma Lucutan Pijar Korona Positif Rahayu, Sri; Azam, M.; Triadyaksa, Pandji; Nur, Muhammad
BERKALA FISIKA Vol 10, No 1 (2007): Berkala Fisika
Publisher : BERKALA FISIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.684 KB)

Abstract

The aim of this research is analysis electrohydrodynamic phenomenon deformation of oil silicon surface by technology of positive corona glow discharge plasma which generated by plasma generator system with point-to-plane electrodes geometry configuration. Variation of voltage and distance between electrodes had been treated to surface of silicon oil. Electrohydrodynamic phenomenon deformation in this research is caused by polarization and ion wind. Ionic wind would be happened if applied voltage over the corona threshold that was 2.6 kV. Graph current of saturation corona unipolar for variation voltage, being formed semi-parabolic equation while the variation of distance between electrodes, being formed linear equation. The hole diameter at variation voltage was influenced by electrics field intensity and momen dipole strength. The hole diameter at variation of distance between electrode was influenced by parabolic angle of electrics line field. The oil velocity for back to initial position is different. Velocity would be go down if the voltage progressively go up and velocity would be go up if the distance between electrode progressively go up.
PEMBENTUKAN SIFAT OPTIS AKTIF MENGGUNAKAN MEDAN RADIO FREQUENCY (RF) PADA LARUTAN GARAM Kamil, Ahmad; Azam, M.; Firdausi, K. Sofjan
BERKALA FISIKA Vol 10, No 2 (2007): Berkala Fisika
Publisher : BERKALA FISIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.76 KB)

Abstract

The non-linear optical property of rotary power of light polarization in water and salt solution has been developed using the Radio-Frequency field (RF-Field). The RF-Field used in the experiment is produced by RF generator, and it supplies the frequency in the range between 6.04 to 14.56 MHz, which then applies to the samples. The source of light with 632.8 nm-wavelength and 532 nm-wave length are used to observe the change of angle polarization of the light, after it passes through the samples. Three modes of the direction of the electric field of light are chosen 0o (E//) (E of light is parallel to E of RF-Field, and 90o (E^) (E of light is perpendicular to E of the RF-Field) in order to determinate the optimal change of the angle of the polarization. The values of  is measured as a function of RF-Field, , where  is the angular frequency of RF-Field and BRF is the magnitude of magnetic field measured in the center of the coil which is assumed to be homogeny in the circumstances of the samples. The results of the experiment show that the degrees of change of the polarization direction, is largest at the  mode. It shows also that at small wavelength of light we obtain the larger, which is indicated the higher frequency of light will more induce the dipoles of molecules of the samples. Keywords: non-linear optics, polarization angle, rotary power
Pembuatan Hologram Refleksi Arifah, Arifah; Firdausi, K. Sofjan; Azam, M.
BERKALA FISIKA Vol 10, No 3 (2007): Berkala Fisika
Publisher : BERKALA FISIKA

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Abstract

A Reflection hologram of a white ceramic object has been made on 5000 lines/mm PFG-03M holographic plate. A He-Ne Laser is used to record the holograms. And to reconstruct, it is used white, blue, and yellow light. The holographic plate for making of reflection hologram is PFG-03M. The optimal exposure time lies between 8 and 10 seconds. By reconstruction image white LED, it shows that the image is formed clearer than using blue and yellow light.   Keywords:  holography, reflection hologram, reconstruction, exposure time.  
Rancang Bangun Pengatur Cermin Sebagai Komponen Gerak Interferometer Pada Spektroskopi FTIR Adi Wibowo, Wahyu; Edi Widodo, Catur; Azam, M.; Firdausi, K. Sofjan
BERKALA FISIKA Vol 11, No 3 (2008): Berkala Fisika
Publisher : BERKALA FISIKA

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Abstract

Michelson's interferometer and mirror regulator system as components of its power have been designed. Interferometers stirred component is one of monotonic mirror on optic system. Interferometer is made accords Michelson's Interferometer attempt. Mirror M1 and mirror M2 is assembled mutually upright then one beam splitter placed before both of mirror. One laser light source is given before mirror M1 crosses beam splitter. There is a detector before mirror M2 crosses beam   splitter upright with laser light source. Mirror M1 is made flexible by assembled gear and stepper's motor to be able forward or backward motion then so called moveable mirror.  Stepper's motor is turned around by computer via parallel port. From interferometer engineering and mirror regulator system already been made, gotten interference pattern with ring amount that the less each mirror gets forward motion and ring amount that more and more each mirror moves to backward. Shift moveable mirror every step for forward or backward is 1,5 µm with distance sails through maximal be 6,0 mm. The in common result point out that interferometer and regulator system of moveable mirror show feature changed interference pattern along with mirror move. Key word: interferometer, moveable mirror, motor stepper, parallel port.
OPTIMASI SHIELDING NEUTRON PADA THERMALIZING COLUMN REAKTOR KARTINI Nurlaili, Fidayati; Azam, M.; Firduasi, K. Sofjan; Widarto, Widarto
BERKALA FISIKA Vol 11, No 4 (2008): Berkala Fisika
Publisher : BERKALA FISIKA

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Abstract

Shield optimization and the calculation of neutron flux the coming out of the thermalizing column of the Kartini reactor have been conducted.The neutron flux will decrease exponentially as it passes the thermalizing column. The thermalizing column of Kartini reactor consists of many different kinds of shields, i.e, graphite, lead, paraffin and an empty chamber of air, therefore the neutron flux count that is coming out of the thermalizing column can be calculated and the shields within the column can be optimized to give smaller  neutron flux.Calculation result indicates that the neutron flux coming out of the thermalizing column is 2,681×10-11 cacah cm-2s-1. After optimization, the remaining flux coming out of the thermalizing column is 1,042×10-28cacah cm-2s-1. Key words: thermalizing column, Shield, neutron flux
Pengaruh Teknik Tegangan Tinggi Terhadap Entrasce Skin Exposure( ESE ) dan Laju Paparan Radiasi Hambur Pada Pemeriksaan Abdomen Dhahryan, Dhahryan; Azam, M.
BERKALA FISIKA Vol 12, No 1 (2009): Berkala Fisika
Publisher : BERKALA FISIKA

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Abstract

The measurement for influence of high voltage technique to Entrance Skin Exposure and  Rate of Scattered Radiation Exposure on abdomen inspection have been conducted. The ESE measurement is conducted using Electrometer and  calculated by semi empirical method, while in measurement of rate of scattered radiation exposure using survey meter at a distance of 100 cm from the object by varying data intake points and its direction of detector. The result indicates that the usage of high voltage technique yields absorbent dose of 124 mrad and radiation exposure of 339 mR. It is lower than the standard value of absorbent dose of 322.7 mrad and radiation  exposure of 130,5 mR. The result of measurement is higher than calculation. In measurement of exposure of scattered radiation rate with detector position faced to object, on right side of cathode, it yields 1.03mR/hour with standard voltage and 0.32 mR/hour with high voltage technique. While in measuring  exposure of scattered radiation rate and back-scattered obtained result on A’ and C’ (close to anoda and side of object) and A” and C” nearly same, with highest value of 1 mR/hour and 0.93 mR/hour at standard tube voltage and at high kV technique obtained lower value of 0.29mR/hour and 0.25mR/hour.
PENGARUH INDUKSI MEDAN RADIO FREQENCY (RF) PADA DIPOL-DIPOL MOLEKUL AIR Firdausi, Ketut Sofjan; Kamil, Ahmad; Azam, Muchammad
JURNAL SAINS DAN MATEMATIKA Volume 17 Issue 1 Year 2009
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3722.702 KB)

Abstract

ABSTRACT-The non-linear optical property of rotary power of light polarization in water has been develop using the Radio-Freguency field (RF-Field). The RF-Field used in the experiment is produced by RF generator, and it supplies the frequency in the range betwen 6,04 to 14,56 MHz which then applies to the samples. The source of light with 632,8 nm-wavelength and 532 nm-wavelength are used to observe the change of range polarization of the light after it passes througt the samples.  Three modes of the direction of the electric field of light are chosen 0o (E//) (E of light is parallel to E of RF-Field), and 90o (E ) (E of light is perpendicular to E of the RF-Field) in order to determinate the optimal change of the angle of the polarization β. The values of β is measured as a function of RF-Field,  , where ωRF is the angular frequency of RF-Field and  BRF is the magnitude of magnetic field measured in the center of the coil which is assumed to be homogeny in the circumstances of the samples. The results of the experiment show that the degrees of change of the polarization direction, η is largest at the E3 mode. It shows also that at small wavelength of light we obtain the larger η, which is indicated the higher frequency of liglt will more induce the dipoles of molecules of the samples. Keywords:  non- linear optics, polarization angle, rotary power
Co-Authors A. Bawono Ahmad Kamil Anak Agung Istri Sri Wiadnyani ARIFAH ARIFAH Catur Edi Widodo Dhahryan Dhahryan Eko Hidayanto Evi Setiawati Fidayati Nurlaili H.P. Kusumaningrum Heri Sugito I. Afiefah Izzah Afiefah Jatmiko Endro Suseno K. Sofjan Firdausi K. Sofjan Firduasi Ketut Sofjan Firdausi, Ketut Sofjan Muhammad Nur Pandji Triadyaksa Qidir Maulana Ririn Widya Septianti Salimatul Litasova Setiyono Setiyono SRI RAHAYU Very Richardina W. S. Budi Wahyu Adi Wibowo Widarto Widarto