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Resistance of Nilaparvata lugens to Fenobucarb and Imidacloprid and Susceptibility to Neem Oil Insecticides Danar Dono; Neneng S Widayani; Safri Ishmayana; Yusup Hidayat; Fitri Widiantini; Ceppy Nasahi
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.234-244

Abstract

This study aims to monitor and determine the level of resistance of N. lugens to fenobucarb and imidacloprid, to analyse of the specific activities of asetylcholinesterase, Glutation S-Transferase, esterase, and to determine sensitivity of resistant N. lugens to the neem oil. Resistance tests were conducted on a field population from Cipunagara, Subang, West Java, Indonesia, and a standard population originating from Banyuwangi, East Java, that was obtained from the Indonesian Center for Rice Research. Synthetic and neem Insecticides were applied using the leaf-stem dipping method. The resistance ratio obtained by comparing the LC50 value of the field population with the standard. The test insects were considered to be resistant to synthetic insecticide if they had a resistance ratio (RR) of more than 4, indicated resistance if RR ≥1, and susceptible to neem if has RR <1. The results of the research showed that the N. lugens population of Cipunagara indicated resistance to fenobucarb (RR = 2.43), while it was clearly resistance to imidacloprid (RR = 13.95). Specific activities of the acetylcholinesterase and Glutathione S-Transferase of the N. lugens Cipunagara populations were higher than the standard, while the esterase specific activity was lower. These two enzymes are responsible for the resistance of N. lugens to fenobucarb and imidacloprid. Nevertheless, the N. lugens population of Cipunagara was susceptible to neem (RR <1) that never been reported before. Therefore, it is important to know the change in the sensitivity of the target and the metabolic pathway of the insecticide used to manage insect resistance.
Cases of Fall Army Worm Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) Attack on Maize in Bandung, Garut and Sumedang District, West Java. Yani Maharani; Vira Kusuma Dewi; Lindung Tri Puspasari; Lilian Rizkie; Yusup Hidayat; Danar Dono
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (925.498 KB) | DOI: 10.24198/cropsaver.v2i1.23013

Abstract

The toxicity of neem (Azadirachta indica), citronella (Cymbopogon nardus), castor (Ricinus communis), and clove (Syzygium aromaticum) oil against Spodoptera frugiferda Danar Dono; Yusup Hidayat; Tarkus Suganda; Syarif Hidayat; Neneng Sri Widayani
CROPSAVER Vol 3, No 1 (2020)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.464 KB) | DOI: 10.24198/cropsaver.v3i1.28324

Abstract

An important pest in corn plantation recently reported in Indonesia was Spodoptera frugiperda, a polyphagous pest that have a fast spread rate and caused high damage on young corn. One control that able to use is botanical insecticide. This experiment aims to determine the toxicity of neem, jatropha, clove, and citronella oil (concentrations of 0.1% and 0.05%) against S. frugiferda in corn. Tests carried out using the feeding assay or residue on corn leaves (size 5 x 5 cm). Then, the leaf treatment was given to 10 larvae of S. frugiferda (instars 2) for 48 hours. After that, the feed replaced with untreated feed. The test results showed that concentration neem oil of 0.1% caused the highest mortality of S. frugiferda. Neem oil exposure is also able to inhibit the time of larval development, reduce the percentage of feed consumption, and the weight of pupa the S. frugiferda. Other vegetable oils exposure gave lower mortality in the test insects and some treatments were not significantly different from the controls on the parameters of larval development, pupae weight, and percentage of feed consumption. Neem oils showed strong toxicity against S. frugiperda larvae with  Lethal Concentration 50 (LC50) of 0.079 % (CI (95%) 0.066 – 0.095%) and LC95 of 0.338% (CI (95%) 0.024-0.567%).
Bioaktivitas Formulasi Minyak Biji Azadirachta indica (A. Juss) terhadap Spodoptera litura F. Raden Arif Malik Ramadhan; Lindung Tri Puspasari; Rika Meliansyah; Rani Maharani; Yusup Hidayat; Danar Dono
Agrikultura Vol 27, No 1 (2016): April, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.974 KB) | DOI: 10.24198/agrikultura.v27i1.8470

Abstract

ABSTRACTBioactivity Formulation of Seed Neem Oil Azadirachta indica (A.Juss) against Spodoptera litura (F)The purpose of this research was to know the influence of neem seed extract formulation (Azadin 50 EC) on the mortality, larvae development, larvae weight, and food consumption of Armyworm (Spodoptera litura). This research used randomized complete design with 6 treatments and 5 replications, i.e. control, formula at concentration of 0.2%, 0.4%; 0.8%; 1.6%; and 3.2%. Correlation of concentration neem seed oil formulation and mortality of test insect was analysed using probit analysis, weight of test larvae presented in mean and standard deviation, and development time and food consumption of test larvae analysed with analysis of varians. Formula Azadin 50 EC had LC50 value of 0.659% (0.550-0.781%) at 12 days after treatment. The mortality increased significantly in pupae stage that caused LC50 value become 0.152%. The formula prolonged development time, decrease the weight of test insect and decrease food consumption by the larvae.Keywords: Lethal concentration, Mortality, Growth derangement, ExtractABSTRAKPenelitian ini bertujuan untuk mempelajari perlakuan formulasi minyak biji mimba Azadin 50 EC terhadap mortalitas, perkembangan larva, bobot larva dan konsumsi pakan ulat grayak (Spodoptera litura F.). Metode Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dengan 5 kali ulangan. Perlakuan tersebut yaitu : Kontrol, konsentrasi formula minyak mimba Azadin 50 EC 0,2%; 0,4%; 0,8%; 1,6% dan 3,2%. Hubungan mortalitas dengan konsentrasi formula dianalisis menggunakan analisis probit, sedangkan data bobot larva, konsumsi pakan dan waktu perkembangan larva dianalisis dengan sidik ragam. Hasil penelitian menunjukkan bahwa formula minyak biji A. Indica memiliki nilai LC50 sebesar 0,659% (0,550-0,781%) terhadap larva instar 2 hingga instar 4. Pada fase pupa kematian kembali meningkat tajam sehingga nilai LC50 menjadi 0,152%. Perlakuan formula tersebut mengakibatkan perpanjangan waktu perkembangan, menurunkan bobot, dan menurunkan konsumsi pakan larva uji.Kata Kunci: Konsentrasi letal, Mortalitas, Gangguan perkembangan, Ekstrak
PENGARUH UKURAN PARTIKEL KAOLIN TERHADAP MORTALITAS, PERTUMBUHAN, DAN PERKEMBANGAN ULAT GRAYAK JAGUNG Widia Herhayulika; Yusup Hidayat; Agus Susanto
Jurnal Penelitian Saintek Vol 26, No 2 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i2.42983

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh ukuran partikel mineral kaolin terhadap mortalitas, pertumbuhan dan perkembangan larva S. frugiperda. Metode penelitian menggunakan Rancangan Acak Kelompok (RAK) dengan 7 perlakuan dan 4 ulangan. Perlakuan terdiri atas formulasi mineral kaolin konsentrasi 4% dengan ukuran saringan 100, 200, 300, 400, dan 500 mesh; kontrol air; dan kontrol wetting agent+dispersant agent. Hasil penelitian menunjukkan bahwa ukuran partikel mineral kaolin berpengaruh terhadap mortalitas, lama perkembangan, berat pupa, dan kemunculan imago S. frugiperda. Mineral kaolin ukuran partikel 500 mesh menghasilkan mortalitas larva S. frugiperda (28,75%) yang berbeda nyata dengan kontrol (0,00%) pada 48 jam setelah perlakuan. Sementara itu, pada pengamatan 23 hari setelah perlakuan, semua ukuran partikel mineral kaolin yang diuji menyebabkan mortalitas larva S. frugiperda sebesar 31,25-51,25%; yang berbeda nyata dengan kontrol (0,00%). Perlakuan mineral kaolin 500 mesh menghasilkan total perkembangan larva dan pupa yang terlama yaitu 22,25 hari; berat pupa paling rendah yaitu 0,1506 g serta kemunculan imago normal yang terendah yaitu 8,00 ekor (dari 20 larva yang diberi perlakuan). Dengan demikian, mineral kaolin pada ukuran 500 mesh dapat diaplikan sebagai bahan pengendali hama S. frugiperda.EFFECT OF KAOLIN PARTICLE SIZE ON MORTALITY, GROWTH, AND DEVELOPMENT OF FALL ARMY WORMThis study aimed to determine the effect of kaolin mineral particle sizes on mortality, growth and develpoment of S. frugiperda larvae. The research method used a Randomized Block Design (RBD) with 7 treatments and 4 replications. The treatments consisted of kaolin mineral formulation concentration of 4% with the particle sizes of 100, 200, 300, 400 dan 500 mesh.Two controls were used namely water and wetting agent+dispersant agent. The results showed that the particle size of the kaolin mineral had an effect on mortality, developmental time, pupal weight and emergence of S. frugiperda adult. Kaolin mineral with a particle size of 500 mesh resulted in mortality of S. frugiperda larvae (28,75%) which was significantly different from the control (0,00%) at 48 hours after treatment. Meanwhile, on observation 23 days after treatment, all tested kaolin mineral particle sizes caused the mortality of S. frugiperda larvae of 31,25-51,25%, which was significantly different from the mortality in control (0,00%). The 500 mesh kaolin mineral treatment resulted in the longest total larval and pupal development (22,25 days), the lowest pupal weight (0,1506 g) and the lowest normal adult emergence (8 of 20 treathed larvae). Thus, the kaolin mineral at a size of 500 mesh can be applied as a pest control material for S. frugiperda.
MEMBANGUN KEMANDIRIAN PANGAN KELUARGA MELALUI PELATIHAN PENANAMAN TEKNIK HIDROPONIK SISTEM SUMBU Sri Ayu Andayani; Yusup Hidayat
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol 2 No 1 (2021)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.786 KB) | DOI: 10.31949/jb.v2i1.658

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Pelatihan penanaman dengan Teknik hidroponik system sumbu dalam membangun kemandirian pangan keluarga bagi ibu rumah tangga yang tergabung dalam sebuah organisasi perempuan di Bandung merupakan tema dari pengabdian kepada masyarakat. Untuk meningkatkan potensi yang produktif dari ibu-ibu rumah tangga selain melaksanakan kewajiban internal rumah tangga juga mampu aktif dan produktif dalam kegiatan public dan ekonomi merupakan maksud dari pemberdayaan ibu rumah tangga. Metode dalam kegiatan pelatihan penanaman Teknik hidroponik system sumbu menggunakan metode partisipatif yaitu sebelum melaksanakan praktek langsung, peserta diberikan pemahaman teori terlebih dahulu. Hasil akhir dari kegiatan pelatihan ini, ibu – ibu peserta berperan aktif dalam mengikuti kegiatan dan diperolehnya sikap, pengetahuan, keterampilan tentang teknik hidroponik system sumbu dan meningkatnya peran ibu dalam membangun kemandirian pangan keluarga juga sebagai penyedia sayuran sehat keluarga.
The effectiveness of vegetable oil formulations in reducing oviposition of Bactrocera dorsalis Hendel (Diptera: Tephritidae) in large red chili fruits Yusup Hidayat; Muthia Riefka Fauziaty; Danar Dono
Jurnal Entomologi Indonesia Vol 15 No 2 (2018): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.15.2.87

Abstract

Chili peppers (Capsicum annuum), in their many varieties, constitute a culturally and economically important horticultural crop in a number of countries. The Indonesian cayenne large red chili (Capsicum annuum var. annuum) is used widely in Indonesia mainly in cooking. There have been reports of increased infestation of large red chili by insect pests, particularly fruit flies. The aim of this study was to investigate the effectiveness of five edible vegetable oils (palm oil, coconut oil, soybean oil, corn oil, and candlenut oil) and one non-edible vegetable oil (neem oil) in reducing landings, oviposition, and infestation by the Oriental fruit fly (Bactrocera dorsalis Hendel) in large red chili fruits. This lab-based experiment entailed exposure of large red chili fruits to 20 mature B. dorsalis females (14–21 days old) inside a 15-l plastic container. Six separate containers each held 10 large red chili fruits with a single oil treatment in each. Prior to exposure, each of the treated and control large red chili fruits was punctured once with a needle in order to create an opening for oviposition. Results indicate that the coconut oil formulation was most effective in preventing damage from B. dorsalis females, and reducing fruit fly landings, oviposition, and infestation.
Respon Hama Penggerek Buah Kopi (Hypothenemus hampei Ferr.) (Coleoptera: Curculinoidae: Scolytidae) terhadap Ekstrak Buah Kopi yang Terinfestasi Hama sebagai Atraktan di Perkebunan Kopi Rakyat Gunung Tilu Siska Rasiska; Sania Safira; Yusup Hidayat; Endah Yulia; Mira Ariyanti
Agrikultura Vol 33, No 3 (2022): Desember, 2022
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v33i3.40277

Abstract

Penggerek buah kopi (PBKo), Hypothenemus hampei Ferr. adalah salah satu hama utama pada tanaman kopi. Persentase serangan hama PBKo pada buah kopi dapat mencapai 100% jika tidak ada pengendalian. Salah satu teknologi pengendalian hama PBKo yang dapat dilakukan adalah penggunaan ekstrak buah kopi yang terinfestasi hama PBKo sebagai atraktan. Penelitian ini bertujuan untuk mengetahui pengaruh esktrak buah kopi yang terinfestasi sebagai atraktan hama PBKo dan memperoleh konsentrasi ekstrak buah kopi terinfestasi yang paling efektif sebagai atraktan imago hama PBKo. Selain itu juga dipelajari serangga arthropoda lain yang tertangkap. Penelitian ini dilakukan di perkebunan kopi rakyat Gunung Tilu, Desa Margamulya, Kecamatan Pangalengan, Kabupaten Bandung, Jawa Barat pada bulan November 2021 hingga Maret 2022. Pembuatan ekstraksi buah kopi terinfestasi dengan menggunakan teknik maserasi dan destilasi air dilakukan di Laboratorium Pestisida dan Toksikologi Lingkungan, sedangkan pengamatan hama dilakukan di Laboratorium Hama Tanaman, Fakultas Pertanian, Universitas Padjadjaran. Penelitian ini menggunakan Rancangan Acak Kelompok dengan 8 perlakuan yaitu P0 = kontrol; P1 = 50 mL air sulingan, P2 = 100 mL air sulingan, P3 = 150 mL air sulingan, P4 = 50 mL ekstrak etanol, P5 = 100 mL ekstrak etanol, P6 = 150 mL ekstrak etanol dan P7 = etanol 10 mL dengan masing-masing perlakuan diulang tiga kali. Hasil penelitian menunjukkan ekstrak buah kopi terinfestasi dengan menggunakan teknik maserasi dan destilasi air tidak dapat menarik hama PBKo serta tidak menurunkan intensitas serangan hama PBKo. Namun, ekstrak buah kopi terinfestasi dapat menarik sejumlah serangga hama dari ordo Diptera famili Drosophilidae dan parasitoid dari ordo Hymenoptera famili Braconidae.