Article Per Year (5 Year)
p-Index From 2019 - 2024
0.23
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Biotropika Indian Journal of Forensic Medicine & Toxicology Journal of Global Pharma Technology
Muhaimin Rifa’i
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 9 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : Jurnal Veteriner

 1 
Aktivitas Imunomodulator Ekstrak Buah Mengkudu pada Mencit yang Diinfeksi Staphylococcus aureus (IMMUNOMODULATORS ACTIVITY OF NONI (MORINDA CITRIFOLIA L.) FRUIT EXTRACT IN MICE INFECTED WITH STAPHYLOCOCCUS AUREUS) Zumrotul Mufidah; Muhaimin Rifa’i; Sri Rahayu
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.221 KB)

Abstract

This study aim was to determine the immunomodulatory activity of noni (Morinda citrifolia L.) fruitextract in mice infected with Staphylococcus aureus. Mice were divided into two group :  non-infected  andinfected. Non Infected group was without S. aureus infection whereas the infected group was infected withS. aureus. Group contain control, dose 1 (25 mg/kg BW), dose 2 (100 mg/kg BW), and dose 3 (300 mg/kg BW).Oral treatment carried out for 20 days in every morning and each sample was injected with  S. aureus atday 21 with 109 cell/mL. Relative number of T cell (CD4+, CD4+CD25+),) and cytokine interferon-ã fromCD4+ T cell (CD4+IFN-ã+) subsets was measured using the BD FACSCaliburTM Flowcytometer. Data wereanalyzed by using Analysis of Varians (p<0,05) and SPSS 16 for windows. The result showed thatadministration of noni crude extract was significantly change the relative number of CD4+, CD4+IFN-ã+,and CD4+CD25+ T cells. Treatment of noni crude extract in non-infection group could increase  relativenumber of CD4+, CD4+IFN-ã+  and CD4+CD25+ T cells that might be caused by active compounds of noni asmitogen.  Giving of noni crude extract in infected group could reduce  the relative number of CD4+, CD4+CD25+and CD4+IFN-ã+ T cells due to it  active compounds as anti-inflamation. Noni fruit extract can be used aspreventive therapy on S. aureus infection  because it contains active compounds as an anti-inflammationeffect.
Aktivitas Ekstrak Daun Kelor Terhadap Sel-T Helper dan Sel-T Sitotoksik pada Mencit yang Diinfeksi Salmonella thypi (ACTIVITY OF AQUEOUS LEAF EXTRACT OF HORSERADISH TREE ON HELPER T- CELL AND CYTOTOXIC T- CELL IN MICE INFECTED WITH SALMONELLA THYPI) Akhmad Fathir; Muhaimin Rifa’i; Widodo .
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.914 KB)

Abstract

Typhoid fever is caused by Salmonella typhi infection, and it is a still problem in many developingcountries, including Indonesia. Typhoid fever occurs due to T cells, immune system, especially CD4+ andCD8+ T cells, are deficient. This condition can cause S. thypi infects human body cells . The study aim wasto evaluate profile CD4+ and CD8+ T cells in mice spleen (Mus musculus) infected with  S. thypi afterinducted with aqueous leaf extract of horseradish tree. An experimental laboratory studiy was conductedusing completely factorial randomized design. Mice were divided into two groups, ie non infection group(induced with aqueous leaf extract of horseradish tree, at dose 0 mg/kg BW, 14 mg/kg BW, 42 mg/kg BWand 84 mg/kg BW) and infection group, the Micewere infected with S. thypi (induced with aqueous leafextract of horseradish tree, at dose 0 mg/kg BW, 14 mg/kg BW, 42 mg/kg BW and 84 mg/kg BW). The resultshowed that aqueous leaf extract of horseradish tree increased the number CD4+  of and CD8+ T cells  in allgroups of mice in conclusion administration of aqueous leaf extract of horseradish tree at high dose havecauses immunosuppressive in immune system. Aqueous leaf extract of horseradish tree have aimmunostimulatory and immunosuppressive functions in CD4+ and CD8+ T cells.
Aktivitas Ekstrak Daun Kelor terhadap Respons Imun Humoral pada Mencit yang Diinfeksi Salmonella typhi (ACTIVITY OF KELOR LEAF EXTRACT ON HUMORAL IMMUNE RESPONSE IN MICE POST SALMONELLA TYPHI INFECTION) Mohammad Hefni; Muhaimin Rifa’i; Widodo .
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.095 KB)

Abstract

The aim of this research was to analyze the activity of  kelor (Moringa oleifera Lam) leaf extract onhumoral immune response in mice infected with Salmonella typhi. Mice were divided into two groups : non-infected and infectedS. typhi groups. Each group was administered orally for 20 days with varied doses ofkelor leaf extract i.e. dose (0 mg/kg BW), dose 1 (14 mg/kg BW), dose 2 (42 mg /kg BW), and dose 3 (84 mg/kg BW).  Then all of the sample in infected groups were injected with 108 cells S. typhi. The humoralimmunity responses were determined by observing the number of lymphoid B cell (B220) and naive Thecell (CD4+CD62L+) by using software BD CellQuest Flowcytometry. The data were analysed using Two-Way ANOVA (P<0.05), with SPSS 16.0 for Windows.  The kelor leaf extract showed imunostimulatoryactivity by significantly improved the number of lymphocyte B cell (B220),  and naive Th Cell (CD4+CD62L+)in mice infected with S. typhi.  The lower doses (dose of 14 mg/kg BW, and 42 mg/kg BW) of kelor leafextracts was more effective than the highest dose (84 mg/kg BW). On the other  hand, the high dose showedimunosupresor activity on naive Teessor Th Cell.  However, immunosupressor activity on naïve Th cell wasobserved on the mice given the highest dose of extract.
Sel T Regulator CD4+CD25+ Mencegah Terjadinya Fenotip Letal pada Mencit Defisiensi CD122 Muhaimin Rifa’i; Widodo -
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.417 KB)

Abstract

Mice with a deficiency of cluster of differentiation/CD122 molecules experience increased memory Tcells. Increased memory T cells are activated and interfered with homeostasis that cause death at the ageof 10 ~ 12 weeks. To clarify whether the expression of CD25 molecules on CD4+ T cells responsible for thedevelopment of lethal phenotypes in CD122-/- mice, we did a transfusion of CD4+CD25+ T cells from normalmice to CD122-/- neonates. Transfusion of purified CD4+CD25+ T cells as much as 3 x 104 can prevent theoccurrence of lethal phenotypes generally experienced by CD122-/- mice. Transfusion of CD4+CD25+ T cellsin CD122-/- neonates cause all of the abnormalities that occur in T cell and leukocyte cells can be preventedand develop into normal. Similarly, the hematocrit that decreased dramatically in CD122-/- mice candevelop normally after receiving a transfusion of CD4+CD25+ T cells. In contras, transfusion of CD4+CD25-T cells in CD122-/- mice did not have the effect of preventing the development of the abnormalities inCD122-/- mice. CD4+CD25+ T cells that are lost in periphery of CD122-/- mice can restore to normal afterreceiving a transfusion of CD4+CD25+ T cells. These results clearly show that the expression of IL-2R?(CD25) on CD4+ T cells become pre-requisite for CD4 T cell population in order to play a role as regulatorcells.
Co-Authors Akhmad Fathir Aryati Haruhiko Suzuki Hideo Tsuboi Iswinarno Doso Saputro Izumi Nakashima Khaled Hossain Moch Sasmito Djati Mohammad Hefni Septin Mauludiyana Swastika Pinca Pinca Widodo - Yoes Prijatna Dachlan Yundari, Yundari Zumrotul Mufidah