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Nutrient Characteristics and Fermented Rice Straw Degradation by Lignolitic TLiD and BopR Inoculums Prihartini, I; Soebarinoto, Soebarinoto; Chuzaemi, S; Winugroho, M
ANIMAL PRODUCTION Vol 11, No 1 (2009): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The research has been conducted to evaluate the nutrition’s characteristic and degradation of rice straw fermented  with lignolitic inokulum in different incubation time.  Rice straw fermentation with lignolitik isolates increase the dry matter and crude protein content compare with dried fermented rice straw. On the other hand, fermentation decrease organic matter, crude fiber, and cellulose contents. The decreasing of OM, CF, and cellulose were lower than lignin. The fermentation with lignolitik inoculum increase the value of DM and OM degradation of rice straw in parameter  non-soluble water fraction but degradable potentially(b), potensial of degradation (a+b) dan degradation time (c) BK dan BO but non significant to parameter water soluble fractions (a).  Fermentation with lignolitik inoculum increase the nutritive value and degradation rice strwa and the best treatment was combination of  TLiD dan  BOpR inoculums in three days incubation. (Animal Production 11(1): 1-7 (2009) Key Words: rice straw, rice straw fermented, lignolitik, lignin biodegradation, nutrition
Effect of white kabesak (Acacia leucophloea Roxb) leaves level in the diet on feed intake and body weight gain of Kacang goat Lawa, E. D. W.; Marjuki, M.; Hartutik, H.; Chuzaemi, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 4 (2017): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.4.255-262

Abstract

This study was conducted to analysis the effect of levels of white kabesak (Acacia leucophloea Roxb.) Willd. leaves in the diet on feed intake, digestibility and body weight gain of Kacang goats. The completely randomized block design using 5 treatments and 5 replications was used in this experiment. The treatments were 5 concentrate feeds containing different levels of white kabesak leaves i.e. 0, 10, 20, 30 and 40% in the dry matter (DM) basis (representing T0, T1, T2, T3 and T4 treatments, respectively). The feeds were set up to contain 11.5-12.5% of crude protein (CP). Concentrate feed and native grass was fed at ratio of 60 : 40 was fed to 5 local male goats (age 1-1.5 years old and initial weight of 16.7±5.0 kg). The results showed that DM, organic matter (OM), and CP intake of T0 was not significantly different from those of T1 goats, but it was significantly higher (P<0.05) than those of T2, T3 and T4. The DM, OM, CP, and crude fiber (CF) digestibility as well as body weight gain in T2 goats were significantly higher (P<0.05) and had feed conversion ratio that was significantly better (P<0.05) compared to those of the other feed treatments. In conclusion, the most optimum level of white kabesak (Acacia leucophloea Roxb.) Willd. leaves used in concentrate feed for goat was 20 %.
White Kabesak (Acacia Leucophloea RoxB) Leaves Utilization in Concentrate on Fermentation Products and In Vitro Gas Production Lawa, E.D. Wie; Chuzaemi, S; Hartutik, Hartutik; Marjuki, Marjuki
Journal of Tropical Life Science Vol 10, No 3 (2020)
Publisher : Journal of Tropical Life Science

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Abstract

This study aimed to evaluate gas production and in vitro fermentation products from feed containing leaves of white kabesak (acacia leucophloea Roxb.)Willd. Feed was composed of a ratio of 60% natural grasses and 40% concentrate. The treatments were used levels of white kabesak leaves in concentrates i.e. 0, 10, 20, 30 and 40% in the dry matter (DM) basis as treatments, R0, R1, R2, R3 and R4 treatments, respectively. The results showed that inclusion of acacia leucophloea leaves in concentrate increased organic matter and crude fiber contents but decreased the crude protein content. Increasing level of acacia leucophloea  leaves in concentrate decreased  gas production (b, ml / 500 mg DM) from 198.29 (R0) to 139.93 (R4). The gas production rate (c, ml /hour) was relatively constant between 0.034 to 0.036 on R0 - R3 and 0.028 on R4. Gas production at 48 hours incubation (y, ml / 500 mg DM) decreased from 153.38 (R0) to 103.23 (R4). The NH3 concentrations ranged from 6.17-7.31 mg / 100 ml and the total VFA was 83.07-91.96 mM. The lowest C2 / C3 ratio was in R2 (2.63). The highest IVDMD was 50.18-67.14% in R0 and the lowest IVOMD was 55.04-71.35% R4. The use of acacia leucophloea leaves at level 20% in concentrates as supplements was more efficient in reducing gas production and in vitro fermentation products.
Pengaruh suplementasi berbagai level daun ketela pohon (Manihot utilissima. Pohl) terhadap produktifitas domba ekor gemuk yang diberi pakan basal jerami jagung (Zea mays) Ndaru, Poespitasari Hazanah; Kusmartono, Kusmartono; Chuzaemi, Siti
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 24, No 1 (2014)
Publisher : Faculty of Animal Science, Universitas Brawijaya

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Abstract

This study aimed to evaluate the effect of cassava leaves supplementation strategy on fat-tailed sheep and to determine appropriate levels of protein derived from cassava leaves. Twenty eight fat-tailed sheep aging of 6-12 months old and weighing of 11-17 kg were used and arranged in a Randomized Complete Block Design (ANOVA) were subjected to the following treatments : (1) P0: maize stover ad libitum + cassava flour + urea (1% of DM intake); (2) P1: maize stover ad libitum + cassava flour + cassava leaves silage (0.5 g CP/kg BW); (3) P2: maize stover ad libitum + cassava flour + cassava leaves silage (1 g CP/kg BW); (4) P3: maize stover ad libitum + cassava flour + cassava leaves silage (1.5 g CP/kg BW); (5) P4: maize stover ad libitum + cassava flour + dried cassava leaves (0.5 g CP/kg BW); (6) P5: maize stover ad libitum + cassava flour + dried cassava leaves (1 g CP/kg BW); and (7) P6: maize stover ad libitum + cassava flour + dried cassava leaves (1.5 g CP/kg BW). Variables measured were feed consumption, nutrients digestibility, digestible nutrient intake, nitrogen retention, blood urea concentration, body weight gain and the number of eggs count. The results showed that cassava leaves supplementation as protein source had a highly significant effect (P <0.01) for digested CP intake and had a significant effect (P<0.05) for CP consumption and body weight gain. There were tendencies of increasing (1) DM intake; (2) OM intake; (3) DM, OM and CP digestibility; (4) digested DM intake, (5) digested OM intake, (6) N-retention; and Body Weight Gain. Supplementation of cassava leaves silage and dried cassava leaves at the level of 1.5 g CP / kg BW could reduce the number of worm eggs by 47.65% and 44.22%. Based on experimental results, it could be concluded that supplementation of dried cassava leaves on the level of 1.5 g CP / kg BW could increase the productivity and also could decrease worm eggs number.   Keywords: fat tail sheep, silage, cassava leaves, productivity
PENGARUH RASIO PUCUK TEBU DAN RUMPUT GAJAH DALAM PAKAN LENGKAP SAPI PERAH TERHADAP PARAMETER FERMENTASI RUMEN DAN PASOK PROTEIN MIKROBA MELALUI PENDEKATAN PRODUKSI GAS IN VITRO Anang Stirtoadiu; Kusmartono Kusmartono; Siti Chuzaemi
The Indonesian Green Technology Journal Vol 1, No 2 (2012)
Publisher : Program Pascasarjana Universitas Brawijaya

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Abstract

Penelitian ini dilaksanakan untuk mengetahui rasio optimal penggunaan pucuk tebu (PT) dan rumput gajah (RG) dalam pakan lengkap sapi perah melalui pendekatan produksi gas in vitro. Penelitian dilaksanakan dengan metode percobaan menggunakan RAK 5 x 5. Perlakuan yang digunakan adalah tingkat rasio PT dan  RG dalam pakan lengkap yang disusun iso protein dari bahan : RG (Pennisetum purpureum) umur pemotongan 43– 56 hari, PT (Saccharum officinarum), dan beberapa bahan konsentrat. Pakan perlakuan disusun dalam 100 % BK dengan perbandingan 40 % BK konsentrat (BKK) dan 60 % BK hijauan (BKH) dimana masing-masing perlakuan dan komposisi BKH adalah: CF1 (100 % RG + 0 % PT); CF2 (75 % RG + 25 % PT); CF3 (50 % RG + 50 % PT); CF4 (25 % RG + 75 % PT) dan  CF5 (0 % RG + 100 % PT). Interval inkubasi pengukuran laju produksi gas : 0, 2, 4, 8, 16, 24, 32, dan 48 jam. Pengukuran efisiensi sintesis protein mikroba (ESPM) ditentukan dengan reflux larutan NDS yang ditambahkan pada residu produksi gas. Hasil penelitian menunjukkan bahwa rasio PT terhadap RG dalam pakan lengkap sapi perah memberikan pengaruh tidak nyata (P>0,05) terhadap volume gas pada inkubasi 2 dan 4 jam, tapi berpengaruh sangat nyata (P<0,01) pada inkubasi 8 – 48 jam. Perlakuan pakan memberikan pengaruh sangat nyata (P<0,01) terhadap degradasi BK, nyata (P<0,05) terhadap kadar total VFA dan asam asetat (C2) namun tidak berpengaruh nyata (P>0,05) terhadap degradasi BO, kadar NH3,  kadar asam propionat (C3), asam butirat (C4), C2/C3, dan proporsi molar VFA. Pakan perlakuan memberikan pengaruh yang tidak nyata (P>0,05) terhadap ESPM (mg N/kg BOTR) dan semua hasil perhitungan stoichiometri. Rasio PT terhadap RG yang semakin tinggi menghasilkan efisiensi sintesis protein mikroba yang relatif sama, efisiensi tertinggi cenderung ditunjukkan oleh level PT sebesar 25 % dan 100 %. Disimpulkan bahwa pada pakan lengkap sapi perah dengan protein kasar 12,5  %; proporsi PT sebagai sumber serat hingga 100 % dapat diaplikasikan. Kontunyuitas dan stabilitas suplai pakan sapi perah dapat dipenuhi dengan memanfaatkan PT namun suplementasi pakan konsentrat sangat disarankan agar   terpenuhi kebutuhan nutrisi, khususnya pasok protein mikroba pada pencernaan pasca rumen. Kata kunci : pasok protein mikroba, rasio pucuk tebu dan rumput gajah, pakan lengkap.
KAJIAN PENGGUNAAN SILASE ISI RUMEN DALAM RANSUM KONSENTRAT SAPI PERAH PERANAKAN FRIESIAN HOLLAND (PFH) TERHADAP PENAMPILAN PRODUKSI SUSU Isnandar Isnandar; Made M. Ardhana; Siti Chuzaemi
The Indonesian Green Technology Journal Vol 1, No 3 (2012)
Publisher : Program Pascasarjana Universitas Brawijaya

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Abstract

Penelitian ini bertujuan untuk mengetahui nilai kimiawi wastelase campuran isi rumen dan bekatul dengan bahan aditif Effective Microorganisme (EM4) dan Tetes, serta pengaruhnya sebagai bahan tambahan ransum konsentrat terhadap penampilan produksi susu sapi perah Peranakan Friesian Holland (PFH) yang dilaksanakan di Balai Diklat Pertanian (BLPP) Batu-Malang. Isi rumen yang digunakan isi rumen sapi berasal dari rumah potong hewan Kotamadya Malang, diambil secara acak selama periode penelitian.  Aplikasi penggunaan silase isi rumen sebagai pengganti konsentrat Bapro dengan pakan basal hijauan jerami jagung muda, diberikan pada sapi perah laktasi sebanyak 20 ekor umur 7 tahun dengan masa laktasi ke lima. Metode percobaan yang dilakukan pada percobaan pertama adalah menggunakan Rancangan Acak Lengkap pola faktorial dengan dua faktor perlakuan , faktor pertama terhadap pembuatan silase terdiri dari perlakuan tanpa penggunaan EM4 (E0) dan dengan penggunaan EM4 (E1) serta penambahan tetes sebagai faktor kedua terdiri dari perlakuan tanpa penambahan tetes (T0) penambahan tetes 1,5 persen (T1) dan penambahan tetes 3 persen (T2), sehingga terdapat 6 kombinasi perlakuan, masing-masing perlakuan diulang 4 kali ulangan. Setiap unit percobaan terdiri dari campuran 70 persen isi rumen dan 30 persen bekatul yang dimasukkan ke dalam kantong plastik polyethylene hitam kedap udara.  Untuk mengetahui nilai nutrisi dilakukan uji kimiawi, pada perlakuan penambahan EM4 dilakukan setelah 12 ensilase sedangkan perlakuan tanpa penambahan EM4 dilakukan setelah 16 hari ensilase.  Parameter yang diamati meliputi kandungan Bahan Kering (BK), Bahan Organik (BO), Protein Kasar (PK), Serat Kasar (SK), Kecernaan Bahan Kering (KcBK) In Vitro, Kecernaan Bahan Organik (KcBO) In Vitro, N-NH3, asam laktat, asam butirat, dan asam asetat, serta kecepatan fermentasinya yang diukur adalah pH.  Percobaan kedua merupakan aplikasi penggunaan silase isi rumen sebagai campuran ransum konsentrat, menggunakan Rancangan Acak Kelompok, kelompok pertama berdasarkan bobot badan sapi ±350 Kg, kelompok kedua ±400 Kg, kelompok ke tiga ±450 Kg, kelompok ke empat ±500 Kg, masing masing kelompok terdiri dari 5 ekor sapi umur 7 tahun dengan periode laktasi ke 5. Setiap kelompok  diberi perlakuan pakan basal hijauan jagung muda dengan konsentrat Bapro sebagai perlakuan kontrol (RKI0), konsentrat Bapro 92,5% dan silase 7,5% (RKI1), konsentrat Bapro 85% dan silase 15% (RKI2), konsentrat Bapro 77,5% dan silase 22,5% (RKI3), konsentrat Bapro 70% dan silase 30% (RKI4), dengan perhitungan ransum iso protein kasar dan iso energi metabolis (ME), dengan kandungan Protein kasar 13,22% dan ME 2,045Mcal/kg.  Perameter yang diamati jumlah produksi susu (liter), kadar lemak susu (%), berat jenis susu, solid non fat (%) dan konsumsi bahan kering. Hasil penelitian pertama menunjukkan bahwa perlakuan penggunaan EM4 dan Tetes berpengaruh sangat nyata terhadap semua parameter yang diamati.  Silase dengan perlakuan penggunaan EM4 mempunyai kandungan BK, BO, PK, KcBK, KcBO asam laktat lebih tinggi  dari pada tanpa penggunaan EM4 sebaliknya kandungan SK, Asam butirat, asam asetat, N-NH3 dan nilai pH lebih rendah.  Perlakuan penambahan tetes 3% mempunyai kandungan BK, BO, PK, KcBK, KcBO dan asam laktat tertinggi, kemudian menurun berturut-turut pada penambahan tetes 1,5% dan tanpa penambahan tetes (0%), sebaliknya kandungan SK, Asam butirat, asam asetat N-NH3 dan nilai pH lebih rendah.  Secara umum perlakuan penambahan tetes 3% dengan penggunaan EM4 0,1% mempunyai nilai nutrisi paling baik.   Hasil penelitian kedua menunjukkan bahwa penggunaan silase isi rumen dari perlakuan penambahan tetes 3% dan penggunaan EM4 0,1%, sebagai pengganti pakan konsentrat sampai dengan 30% dari yang dikonsumsi tidak berpengaruh nyata terhadap penampilan produksi susu sapi perah.  Sedangkan kelompok bobot badan dan perlakuan ransum konsentrat berpengaruh  sangat nyata terhadap konsumsi bahan kering. Silase isi rumen terbaik yang dihasilkan dari perlakuan penggunaan EM4 dan level penambahan tetes 3 persen, dapat digunakan sebagai pengganti salah satu bahan campuran konsentrat dengan kandungan nilai nutrisi yang sama. Kata kunci : Silase, Isi rumen, Konsentrat, Sapi Perah Peranakan Friesian Holland, Produksi Susu
EFFECT OF WHEAT POLLARD (Triticum aestivum) AND SOYBEAN MEAL (Glycine max) PROTECTED WITH CONDENSED TANNIN IN THE DIET ON FEED INTAKE AND DIGESTIBILITY OF LACTATION DAIRY COW Siti Chuzaemi; Mashudi Mashudi; Marjuki Marjuki; Asri Nurul Huda
TERNAK TROPIKA Journal of Tropical Animal Production Vol 15, No 1 (2014): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Abstract

The objectives of this study were to investigate the effect of wheat pollard (Triticum aestivum) and soybean meal (Glycine max) protected by condensed tannin (CT) on feed intake and organic matter digestibility of lactation Holstein-Friesian Crossbred cows. Twelfth lactation cows were divided into 4 groups based on the month of lactation and each group consist of 4 diet treatments which were supplemented by CT. The using of CT was 6% from total dry matter of wheat pollard and soybean meal depending on the feeding treatments. Feeding treatments consist of T1 : 65% corn leaves (Zea mays)+ 35% concentrate (control 0% CT); T2 : 65% corn leaves + 35% concentrate (soybean meal protected by 6% CT); T3 : 65% corn leaves + 35% concentrate (wheat pollard protected by 6% CT); T4 : 65% corn leaves + 35% concentrate (soybean meal and wheat pollard protected by 6% CT).  Cows were allowed a week of adaptation period, 10 days of preliminary period and 30 days of data collection. Dietary supplemented by 6% CT did not affect feed intake and organic matter digestibility. These results suggest continuing the next experiment to determine the effect of CT on ruminant animals with higer level of CT and the other parameters.   Keywords : condensed tannin (CT), wheat pollard, soybean meal, feed intake, digestibility, lactation dairy cows
EFFECT OF FLOUR AND PAPAYA LEAF EXTRACT (CaricapapayaL) IN FEED TO AMMONIA CONCENTRATION, VOLATILE FATTY ACIDS AND MICROBIAL PROTEIN SYNTHESIS IN VITRO Pipin Sairullah; Siti Chuzaemi; Herni Sudarwati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 17, No 2 (2016): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.792 KB) | DOI: 10.21776/ub.jtapro.2016.017.02.9

Abstract

This research was conducted to investigate the effect of flour and papaya leaf extract (Carica papaya L) on a mixed diet of napier grass (Pennisetum purpureum Schaum) and pollard (Triticumaestivum) to the concentration ammonia, Volatile Fatty Acids (VFA’s) and microbial protein synthesis invitro. This research method was an experiment in laboratory with Randomized Block Design (RBD) using 5 treatments with 3 replications. the treatment were P0 : Control Ration (napier grass + pollard ratio of 1:1 in the DM), P1 : P0 + 2.0% papaya leaf flour DM, P2 : P0 + 4.0% papaya leaf flour DM, P3 : P0 + extract DM 2.0% papaya leaves, P4 : P0 + extract DM 4.0 % papaya leaves. The measured variables were concentration of ammonia incubating rumen fluid 24 hour, concentration of VFA’s and microbial protein synthesis in the rumen fluid incubation 48 hour. Data were analyzed using variance (ANOVA), if there any differences in treatment, then continued with Duncan Multiple Range Test (DMRT). The results of research that provides highly significant difference (P <0.01) the concentration of ammonia and microbial protein synthesis, but not significantly (P> 0.05) against the VFA’s. Conclusion of the research was the additional flour and papaya leaf extract on blanding of napier grass and pollard can increase the concentration of ammonia, VFA’s, and microbial protein synthesis. The best treatment is the addition of papaya leaf flour 4.0 % (P2) because it can increase the concentration of ammonia and microbial protein synthesis, treatment with the addition of papaya leaf extract 2.0% (P3) can disposed increase the VFA’s. The suggestion of this research is that papaya leaf flour 4,0 % (P2) could be added on the woof because it can improve ammonia concentration and synthesis protein of microbe, also this is need to do a deep research to know what the effect of additional of flour and papaya leaf extract on protozoa population based on in vitro. Keywords : Papaya leaf, ammonia, Volatile Fatty Acids, in vitro.
Kajian Suplementasi Zinc Organik Terhadap Kualitas Semen Pejantan Sapi Bos indicus Sri Hindrawati; Gatot Ciptadi; Siti Chuzaemi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 21, No 2 (2020): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2020.021.02.7

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi Zinc organik terhadap kualitas semen sapi Bos indicus. Materi penelitian yang digunakan adalah 12 (dua belas) ekor sapi pejantan dan semennya. Sapi pejantan dengan kisaran umur 3-7 tahun dan bobot badan 600-800 kg, diberikan suplementasi Zinc organik[ZN: Availa®Zn 100, mengandung 10% Zinc dari asam amino Zinc kompleks (Zinpro Corporation, Asosiasi Pakan Amerika Pejabat Kontrol (AAFCO) No. 57.150) sebanyak 3 gram/ekor/hari. Pemberian Zinc organik selama 20 minggu dengan pakan basal berupa pakan komplit. Analisis statistik menggunakan T-test independent untuk membandingkan data perlakuan control dan suplementasi Zinc pada masing-masing kelompok sampel pada taraf kepercayaan 95% (α 0,05). Hasil penelitian kualitas semen kelompok kontrol sebagai berikut: pH 6,43±0,05; volume 6,22±1,38 mL; motilitas 77,31±0,04 %; abnormalitas6,18±0,03%; dan konsentrasi 1.403,47±157,21 juta/ml.Sementara hasil penelitian kualitas semen kelompok suplementasi sebagai berikut: pH 6,46±0,07; volume 6,29±1,79 mL; motilitas 79,12±0,04 %; abnormalitas 5,27±0,01%; dan konsentrasi 1.302,28±274,84 juta/mL. Kesimpulan dari penelitian menunjukkan bahwa suplementasi Zinc memberikan perbedaan tidak nyata (P<0,05) terhadap pH semen, motilitas, abnormalitas, volume semen dan konsentrasi spermatozoa, namun terlihat kecendrungan peningkatan pada pH semen, motilitas, volume semen dan kecendrungan penurunan pada abnormalitas dan konsentrasi spermatozoa.
EFFECT OF FLOUR AND PAPAYA LEAF EXTRACT (Carica papaya L.) ADDITION TO FEED ON GAS PRODUCTION, DIGESTIBILITY AND ENERGY VALUES IN VITRO Miftahul Khoiriyah; Siti Chuzaemi; Herni Sudarwati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 17, No 2 (2016): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.735 KB) | DOI: 10.21776/ub.jtapro.2016.017.02.10

Abstract

The purpose of this research was to analyze the effect of  papaya  (Carica papaya L) leaf  extract and flour on a mixed feed made from elephant grass (Pennisetum purpureum) and pollard(Triticum aestivum) on gas production, in vitro digestibility dry matter (DDM), digestibility organic matter (DOM), and the value of energy. The materials used in this research was mixed feed consisting of grass, pollard and papaya leafs as feed supplement. The method used is a Randomized Block Design with 5 treatments and 3 replications. P0 was control feed, P1 was P0 + 2% of papaya leaf flour, P2 was P0 + 4% of papaya leaf flour, P3 was P0 + 2% papaya leaf extract, and P4 was P0 + 4% papaya leaf extract. Duncan's Multiple Range Test was carried out if there found any difference in treatments. The result of the research shows that papaya leaf extract and flour did not give significant influence (P> 0,05) for gas production, in vitro digestibility dry matter and digestibility organic matter , and energy value , but gave significant influence (P <0,01) for net energy. The addition of 4% papaya leaf extract gave the best results for gas production value (value b = 133,16 ml/500 mgDM and value c=0,064 ml/h), digestibility dry matter and digestibility organic value (DDM = 63,57 % and DOM = 65,49 %), metabolizable  energy 8,47 MJ/KgDM value and net energy 6,29 MJ/KgDM value. Those can be concluded that addition of 2% and 4% papaya leaf extract and papaya flour can’t increase gas production, in vitro digestibility of dry matter and organic matter but increases net energy value. Suggestions from this research was that feeding a mixture of grass and pollard added with 4% of papaya leaf flour should be conducted to determine the level of productivity. Keywords : Papaya leaf, gas production, in vitro digestibility, energy