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All Journal ACTA VETERINARIA INDONESIANA Jurnal Manajemen dan Agribisnis Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Kesehatan Lingkungan indonesia BERITA BIOLOGI Jurnal Medik Veteriner JURNAL KAJIAN VETERINER Livestock and Animal Research Jurnal Kedokteran Hewan

Herwin Pisestyani

Divisi Kesehatan Masyarakat Veteriner Dan Epidemiologi, Sekolah Kedokteran Hewan Dan Biomedis, Institut Pertanian Bogor

Author-ID : 2480260

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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GEJALA KLINIS DAN PATOLOGI ANATOMI PASCA INFEKSI Campylobacter jejuni PADA AYAM BROILER [Clinical Symptoms and Pathology Anatomy due to Infection Campylobacter jejuni on Broilers] Andriani, Andriani; Sudarwanto, Mirnawati; Setiyaningsih, Surachmi; Kusumaningrum, Harsi Dewantari; Pisestyani, Herwin
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Campylobacter spp. is a bacteria foodborne zoonotic agent that can infect humans and animals, especially birds. This bacteria can cause important problems in the public health since the beginning at the farm level, the level of retail and on the table. Poultry meat is the main source of contamination because the digestive tract of poultry is the predilection of Campylobacter jejuni. During this infection C. jejuni in poultry did not show typical clinical symptoms, making detection quite difficult to diagnose this disease at the farm level. Incidence infection of Campylobacter sp. in developed countries had 5-90%. Currently poultry production and consumption are predicted to still increase due to a high demand for low price protein. This research is conducted to determine the clinical and pathologic anatomy due to an infection of C. jejuni on chicken digestive tract. A total of 105-day-old chickens are divided into 3 groups, each consisting of 35 individuals. Group A was the control group (not infected) group B (infected with C. jejuni ATCC 33291), and group C (infected with field isolate of C. jejuni/C1). Infection of the chicken is done 0.5 ml peroral suspension concentration of104cfu/ml. Anova Duncan is used to analyze parameters of body weight, increase weight gain, feed consumption, FCR values. Kurskall Walis method used to analyze the parameters non-parametric form of the score changes in the macroscopic and microscopic intestinal and liver. Infection of C. jejuni changes the pathology anatomy of intestine i.e. hyperemia, enteritis and enteritis catharalis haemorrhagic. A microscopic change of intestinal is oedema,haemorrhage and infiltration of inflammatory cell. Changes of pathology anatomy liver are colour smeary, congesti, pale, fragile and necrotic. Microscopic changes of liver are degeneration and focal hepatic necrosis. Group observation infected by C. jejuni caused chicken cannot reach maximum body weight and can be detrimental to farmers because of increase of the value of the feed conversion ratio (FCR).

Identifikasi Escherichia coli Penghasil ESBL dari Efluen Rumah Potong Hewan Unggas di Kota Bogor, Jawa Barat Yunindika, Thufeil; Latif, Hadri; Pisestyani, Herwin; Wahyudi, Ading; Ahmad, Hasniah; Susanti, Oli
Jurnal Kesehatan Lingkungan Indonesia Vol 21, No 1 (2022): Februari 2022
Publisher : Master Program of Environmental Health, Faculty of Public Health, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkli.21.1.43-49

Latar belakang: Escherichia coli merupakan bakteri enterik komensal yang mudah resistan terhadap antibiotik karena memiliki kemampuan bawaan dalam menghasilkam enzim resistansi antibiotik, salah satunya Extended Spectrum Beta Lactamase (ESBL). Bakteri ini dapat ditularkan melalui lingkungan, salah satunya melalui cemaran efluen dari rumah potong hewan unggas (RPH-U) dan Tempat Pemotongan Ayam (TPA). Tujuan penelitian ini adalah untuk mengisolasi, mengidentifikasi, dan mengukur jumlah bakteri E. coli penghasil ESBL dari RPH-U/TPA di Kota Bogor.Metode: Disain penelitian yang digunakan adalah penelitian eksploratif dengan metode pengambilan sampel secara purposive sampling. Sampel yang diambil berjumlah 12 dari tiga RPH-U/TPA yang memiliki kapasitas pemotongan lebih dari 1000 ekor/hari. Metode yang digunakan adalah ESBL Ec Tricycle dan dilakukan di laboratorium Balai Pengujian Mutu dan Sertifikasi Produk Hewan. Data dibahas secara deskriptif dan disajikan berupa gambar dan tabel.Â Hasil: Escherichia coli penghasil ESBL teridentifikasi dari semua 12 sampel yang berasal dari tiga RPH-U/TPA di Kota Bogor dengan persentase E.coli penghasil ESBL terendah 10,45% dan terbesar yaitu 39,52% dengan rata-rata 17,76%.Simpulan: Escherichia coli penghasil ESBL ditemukan pada efluen dari ketiga RPH-U/TPA di Kota Bogor. Â Bakteri tersebut berisiko mencemari lingkungan melalui efluen dan dapat menjadi ancaman kesehatan terutama terhadap masyarakat yang berada di sepanjang aliran sungai. Langkah mitigasi dan pencegahan perlu dilakukan.Â Title: Identification ofÂ ESBL Producing Escherichia coli Â from Poultry Abattoir Effluent in Bogor, West Java Background: Escherichia coli is a commensal enteric bacteria that is easily resistant to antibiotics because it has an innate ability to produce antibiotic resistance enzymes, one of which is Extended Spectrum Beta Lactamase (ESBL). These bacteria could been transmitted through the environment from poultry abattoir (RPH-U) and chicken slaughterhouses (TPA) effluent contamination. The purpose of this study was to isolate, identify, and enumerate the number of ESBL-producing E. coli bacteria from RPH-U/TPA in Bogor CityMethod:Â The research design used is exploratory research with purposive sampling method. 12 samples were taken from 3 RPH-U/TPA which have a slaughtering capacity of more than 1000 head/day. The method used is the ESBL Ec Tricycle and is carried out in the Center for Quality Testing and Certification of Animal Products laboratory. Data discussed descriptively and presented in the form of pictures and tables.Result: Escherichia coli producing ESBL was identified from all 12 samples from 3 RPH-U/TPA in Bogor City with the smallest ESBL-producing E.coli percentage of 10.45% and the largest 39.52% with an average of 17.76%.Conclusion: Escherichia coli producing ESBL was found in the effluent of the three RPH-U/TPA in Bogor City. These bacteria are at risk of contaminating the environment through effluents and can pose a health threat, especially to people living along the river. Mitigation and prevention measures need to be taken.Â

Kesempurnaan Kematian Sapi setelah Penyembelihan dengan dan tanpa Pemingsanan Berdasarkan Parameter Waktu Henti Darah Memancar Herwin Pisestyani; Nadhear Nadadyanha Dannar; Koekoeh Santoso; Hadri Latif
Acta VETERINARIA Indonesiana Vol. 3 No. 2 (2015): Juli 2015
Publisher : IPB University

Parameter untuk mengetahui hewan sapi sempurna setelah disembelih yaitu dengan melihat refleks kelopak mata dan atau waktu henti darah memancar. Menurut EFSA (2004) kematian merupakan suatu keadaan yang ditandai dengan respirasi fisiologis dan sirkulasi darah telah berhenti sebagai akibat dari pusat sistem tersebut di batang otak secara permanen kehilangan fungsi karena kekurangan oksigen dan energi. Waktu henti darah memancar merupakan indikasi bahwa jantung sudah tidak dapat memompa darah keluar dari tubuh karena tidak ada lagi asupan oksigen darah dalam jantung, sehingga hewan tersebut dapat dikatakan mati. Tujuan dari penelitian ini untuk menghitung waktu henti darah memancar pada penyembelihan sapi dengan metode pemingsanan dan tanpa pemingsanan yang dipotong di rumah potong hewan ruminansia besar (RPHRB), sehingga diperoleh data rataan waktu hewan mati sempurna. Tiga puluh ekor sapi Brahman Cross dibagi menjadi 2 kelompok perlakuan yaitu, sebanyak 15 ekor yang disembelih dengan pemingsanan (kelompok 1) dan sebanyak 15 ekor yang disembelih tanpa pemingsanan (kelompok 2). Waktu henti darah memancar dihitung sesaat setelah hewan disembelih sampai darah berhenti memancar. Hasil dari penelitian diperoleh rataan waktu henti darah memancar pada sapi yang dipingsankan sebelum disembelih adalah sebesar 3,02 menit dan rataan waktu henti darah memancar pada sapi yang disembelih tanpa pemingsanan adalah sebesar 2,13 menit. Selang waktu henti darah memancar antara sapi yang dipingsankan dengan sapi yang tidak dipingsankan sebelum disembelih adalah 53,4 detik. Waktu henti darah memancar dipengaruhi oleh perlakuan hewan sebelum pemotongan, yaitu dengan atau tanpa pemingsanan.Kata kunci: Pemingsanan, sapi, tanpa pemingsanan, waktu henti darah (The Perfect Cow Died after Slaughtered by Stunning and Non Stunning Methods According to Gushing Blood Downtime)Palpebra reflex and gushing blood downtime can be used as parameters to see animals death after slaughtered. Stop bleeding time was an indication that the heart is unable to pump blood out of the body due to no more oxygen in the blood of the heart, so that the cattle can be said has been dead perfectly. The aims of this study was to calculate the stop bleeding time of cattle slaughtered by stunning and non stunning methods, thus obtained the avaraging data of perfectly death time of animals. Thirty catlles’s Brahman Cross divided into two treatment groups, firstly 15 cattle’s were slaughtered by stunning method (group 1) and the second one 15 cattle’s were slaughtered by non stunning method (group 2). Blood gushing downtime was calculated immediately after the animal is slaughtered until the blood stops radiating. The results showed the average blood gushing downtime in cattles that were stunning before slaughtered is 3.02 minutes and the average time to stop blood gushing in cattles of non stunning group is 2.13 minutes. The interval blood gushing downtime between the cattles slaughtered by stunning and non stunning was 53.4 seconds. Blood gushing downtime was affected by the treatment of animals before they were slaughtered.Keywords: cattle, gushing blood downtime, non stunning, stunning.

Escherichia coli O157:H7 Resistan Antibiotik Pada Daging Kebab yang Dijual di Sekitar Kampus IPB Dramaga Bogor Devi Yanti Sari; Herwin Pisestyani; Denny Widaya Lukman
Acta VETERINARIA Indonesiana Vol. 9 No. 3 (2021): November 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.3.179-186

Kebab merupakan salah satu makanan siap saji atau ready to eat (RTE) yang populer di seluruh dunia. Escherichia coli (E. coli) O157:H7 banyak dihubungkan dengan kejadian outbreak foodborne disease pada kebab. Kontaminasi E. coli O157:H7 resistan antibiotik pada kebab dapat menimbulkan masalah kesehatan serius. Penelitian ini bertujuan mengidentifikasi E. coli O157:H7 resistan antibiotik yang diisolasi dari daging kebab yang dijual di sekitar Kampus IPB Dramaga Bogor. Total 43 sampel daging kebab diambil dari seluruh pedagang kebab di sekitar Kampus IPB Dramaga dalam radius 2 km dari batas terluar Kampus. Isolasi dan identifikasi E. coli mengacu pada Standar Nasional Indonesia (SNI) 2897:2008 dari Badan Standardisasi Nasional tentang Metode Pengujian Cemaran Mikroba dalam Daging, Telur, dan Susu, serta Hasil Olahannya. Uji serotyping E. coli O157:H7 menggunakan uji Serologis. Uji resistansi E. coli O157:H7 mengacu pada standar Clinical Laboratory Standards Institute (CLSI) dan dilakukan terhadap 10 jenis antibiotik menggunakan metode Kirby-Bauer disk diffusion. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan enam isolat positif E. coli O157:H7 (31.6%; 6/19) yang resistan terhadap ampisilin, amoksisilin-asam klavulanat, sefotaksim, gentamisin, siprofloksasin, enrofloksasin, kolistin sulfat dengan satu isolat termasuk multidrug resistant (MDR). Semua isolat E. coli O157:H7 masih sensitif terhadap trimethoprim-sulfametoksasol, oksitetrasiklin, dan kloramfenikol.

Postmortem Changes in pH, Color, Drip Loss, and Non-Protein Nitrogen in Beef Liver and Lungs During Storage in Refrigerator Denny Lukman; Herwin Pisestyani; Hadri Latif; Etih Sudarnika; Mirnawati Bachrum
Acta VETERINARIA Indonesiana Vol. 8 No. 3 (2020): November 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.8.3.56-63

Beef offal are consumed by people in some countries specifically in Asia. Beef liver and lungs are favorite food which are used as meat in traditional food. The objective of this study was to determine the postmortem changes in pH, color, drip loss, and non-protein nitrogen (NPN) content in beef liver and lungs during storage in refrigerator (3-4 ºC) until 5 d (120 h) after slaughter. The beef liver and lungs were collected from the abattoir and transported in cool box (<7 ºC) to the laboratory within 3 hours. The samples size of beef liver and lungs were 20 for each observation time. In the laboratory the beef liver and lungs were measured directly for pH value, color (L*, a*, and b*), drip loss, and NPN content at 4 h postmortem (pm) and afterwards every beef liver sample was sliced into 5 pieces of 100-120 g and stored in chiller of 3-4 ºC. The measurement of pH, color (L*, a*, and b* values), drip loss, and NPN content were conducted at 4 h, 24 h, 48 h, 72 h, 96 h, and 120 h postmortem. Data were analyzed descriptively and by comparing the 95% confidence interval of mean of each observation. The results showed that pH, color, drip loss, and NPN content in beef lungs were higher than the values in beef liver. The pH of beef liver and lungs declines until 96 h pm and 48 h pm, respectively. The L*, a*, and b* values of beef liver and lungs increased in general during storage. Drip loss and NPN in beef liver and lungs tended to increase significantly during storage. From this study the pH value and NPN can be used to determine the freshness of beef liver and lungs.

Manpower Planning in The Application of Teat Dipping To Control Sub Clinical Mastitis in The Small Dairy Farms Raden Isma Anggraini; Etih Sudarnika; Herwin Pisestyani; Ardilasunu Wicaksono; Mirnawati Sudarwanto
Jurnal Manajemen & Agribisnis Vol. 17 No. 1 (2020): JMA Vol. 17 No. 1, March 2020
Publisher : School of Business, Bogor Agricultural University (SB-IPB)

The application of teat dipping to control subclinical mastitis especially in small dairy farms is important; however, the application needs additional activity that has an impact on labor usage. This paper aimed to describe the labor usage to apply teat dipping for subclinical mastitis control in the small dairy farms. The convenience sampling techniques applied to select 34 farmers in Bogor as the respondents. Workload analysis and labor productivity were used in examining the application of dot dipping in various small dairy farms in Bogor City to control subclinical mastitis. This study was based on two types of experiments in small dairy farms in Bogor, West Java, namely teat dipping and without teat dipping. The findings show that the application of teat dipping had an impact on three things, namely an increase in labor usage and its workload, an increase in labor productivity and a decrease in the number of subclinical mastitis prevalence. This study concluded that labor was an important factor to control subclinical mastitis in small dairy farms. An increase in labor productivity had an impact on reducing the costs of mastitis treatment and finally increase farmer’s income. Keywords: mastitis, labor, small dairy farms, teat dipping, workload analysis

JSPS-9 Microbiologial Quality of Chicken Carcasses in Bogor Indonesia Based on Campylobater sp. and Salmonella sp. Counts Herwin Pisestyani; Elisabet Tangkonda; Maya Shofa; Surachmi Setyaninigsih; Denny Widaya Lukman; Bambang Pontjo Priosoeryanto; Naoaki Misawa
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.528 KB)

Unhygienic handling chicken carcasses during slaughtering until selling to costumers can lead to contamination by pathogenic bacteria such as Campylobacter sp., and Salmonella sp. entering human’s body can cause foodborne diseaseThe aims of this study were to detect contamination and enumuration of Campylobacter sp, and Salmonella sp. in chicken carcasses from poultry slauhterhouses and markets in Bogor Indonesia.

Role of Teat Dipping After Milking for Subclinical Mastitis Control and Improving Production of Dairy Cow Ardilasunu Wicaksono; Etih Sudarnika; Herwin Pisestyani; Mirnawati Sudarwanto; Abdul Zahid; Arifin Budiman Nugraha; Muhammad Pauzi Lubis; Iccha Pradipta Patsiwi
Buletin Peternakan Vol 43, No 2 (2019): BULETIN PETERNAKAN VOL. 43 (2) MAY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i2.30380

This study was aimed to compare somatic cell count and milk production on dairy cattle after teat dipping post milking application. Samples collected from total of 121 quarters of dairy cattle in normal lactation period and identified as infected by subclinical mastitis. Subclinical mastitis examination was done by IPB Test Mastitis Test, somatic cell count calculation was done by Breed Method, and observation of milk production was done by calculating milk volume per quarter. Observations were performed during normal lactation periods of 12 weeks. Data were analyzed statistically using Mann-Whitney Test and unpaired t-test. The results showed that the number of somatic cells count in the treatment group with post milking teat dipping application was lower than in the control group and significantly different at week 11 (p = 0.039). Furthermore, the volume of milk production in the treatment group was higher than in the control group and significantly different in almost all weeks of observation (p<0.05). It was showed that post milking teat dipping application can prevent subclinical mastitis infection effectively, seen from low somatic cell count (below 400.000 cells/ml) and can maintain optimum milk production. So that, this application can increase the economic benefits for dairy farmers.

Gambaran Escherichia coli resistan antibiotik asal tangan pemerah, ambing sapi, susu serta air di Peternakan Sapi Perah Kecamatan Cendana, Enrekang, Sulawesi Selatan Milawarni Milawarni; Herwin Pisestyani; Denny Widaya Lukman
Livestock and Animal Research Vol 20, No 3 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v20i3.61288

Objective: This study aimed to describe the resistance of E. coli to several types of antibiotics isolated from water, milker hand swabs, cow udder swabs and milk in the Enrekang dairy farm, South Sulawesi.Methods: Isolation and identification of E. coli refers to SNI 2897:2008 concerning Methods for Testing Microbial Contamination in Meat, Eggs and Milk, and their Processed Products. The test of E. coli resistance to antibiotics by disc diffusion method and interpretation of the results refers to the Clinical and Laboratory Standards Institute (CLSI). The samples were 13 milker hand swabs, 52 cow udder swabs, 52 individual milks, and 13 water used for rearing activities.Results: E. coli isolates (n=64) consisted of 7 isolates from hand swabs, 7 isolates from milking hands, 20 isolates from udder swabs, 31 isolates from milk, and 6 isolates from water. E. coli isolates were resistant to erythromycin and ampicillin as much as 78%, trimethoprim-sulfamethoxazole 34.4%, tetracycline 31%, ceftazidime 25%, chloremphenicol 22%, enrofloxacin 9.4%, and gentamicin 4.6%. The pattern of E. coli resistance to antibiotics showed that the isolates of E. coli multiple drugs were resistant to up to 7 antibiotics at once.Conclusions: E. coli isolates from milk, cows, milk and water in the Enrekang dairy farming area, South Sulawesi has developed resistance to several types of antibiotics and have the potential to spread resistance genes to other bacteria and threaten human, animal and environmental health.

Microbiological Quality of Milk Flavored Ready to Drink and Hygiene Practice of Street Vendors Herwin Pisestyani; Irena Ivania; Ardilasunu Wicaksono; Denny Widaya Lukman; Mirnawati Sudarwanto
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60996

Milk products consumption has become a new trend of healthy lifestyle among students in order to meet appropriate daily nutrients. This study was aimed to measure the total number of microbes found in milk drinks sold around the IPB Dramaga and Cilibende and to determine factors that influence it. Samples were collected from 13 milk vendors within radius of 2 kilometers from outermost point of campus and taken with 3 repetitions. Calculation of the total number of microbes was done by using the plate count method in accordance with SNI 2897-2008 about testing method of microbial contamination in meat, eggs, and milk, as well as processed products and SNI 01-6366-2000 about the maximum limit of microbial contamination in food. Risk factors’ data was taken by interviewing milk drinks seller using structured questionnaire. Factors that influenced microbial contamination were analyzed using Chi-Square test. The results showed that 74.4% of milk drinks samples had a high total number of microbes. The average total number of microbes in Dramaga and Cilibende samples was 1.0 x 105 ± 1.4 x 105 cfu / ml and 1.1 x 107 ± 1.2 x 107 cfu/ml. The total number of microbes are significantly influenced (p<0.05) by equipments used, the cleanliness of the equipment, the cleanliness of the table, washing hands practice, and the distance from the crowd. The high number of microbial contamination in milk drinks could damage the quality of milk and shorten the shelf life.

Co-Authors Abdul Zahid Agik Suprayogi Ahmad, Hasniah Andriani Andriani Ardilasunu Wicaksono Arifin B. Nugraha Bambang Pontjo Priosoeryanto Chaerul Basri Denny Lukman Denny Widaya Lukman Denny Widaya Lukman Denny Widaya Lukman Denny Widaya Lukman Devi Yanti Sari Etih Sudarnika Gabryella Fransina Amalo Hadri Latif Iccha Pradipta Patsiwi Indra Permana Irena Ivania Jannatun Delvia Koekoeh Santoso Maya Shofa Milawarni Milawarni Mirnawati Bachrum Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Muhammad Pauzi Lubis Nadhear Nadadyanha Dannar Naoaki Misawa Nugraha, Arifin Budiman Raden Isma Anggraini Surachmi Setiyaningsih Surachmi Setyaninigsih Susanti, Oli Tangkonda, Elisabet Trioso Purnawarman Wahyudi, Ading Wicaksono, Ardilasunu Yunindika, Thufeil

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