Garuda - Garba Rujukan Digital

PENGARUH BERBAGAI KONDISI PREPARASI DAN PENYIMPANAN SUSU FORMULA PADA PERTUMBUHAN SPORA Bacillus cereus DAN Clostridium perfringens [The growth of Bacillus cereus and Clostridium perfringens spores under a variety of preparation and storage condition] Purwanti Maya; Mirnawati Sudarwanto; Winiati P. Rahayu; A. Winny Sanjaya
Jurnal Teknologi dan Industri Pangan Vol. 20 No. 1 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.383 KB)

The aim of this study was to evaluate the ability of Bacillus cereus and Clostridium perfringens spores to survived in different preparation temperature (25, 35, and 70°C for B. cereus and 25, 45, 70ºC for C. perfringens) and modified storage condition (with 50 and 75% humidity; opened, closed and opened twice a day during period of observation) of powdered milk formula. The spores of B. cereus ATCC 13061 and C. perfringens CP-1 artificially contaminated to the milk formula. Results showed that B. cereus and C. perfringens spores were germinated and growth in reconstituted milk formula. Bacteria population increased ≥1 log within 3 hours at room temperature (28-29ºC). The spores of both bacteria survived from dry condition of powdered milk formula, like when aw of the formulas increased cause of storage condition.

Effects of pH, Nacl and Teating on the Antibacterial Stability of Kecombrang Rifda Naufalin; Betty Sri Laksmi Jenie; Feri Kusnandar; Mirnawati Sudarwanto; Herastuti Sri Rukmini
Jurnal Teknologi dan Industri Pangan Vol. 17 No. 3 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.949 KB)

The effect of pH (4-9), NaCl concentration (1-5%), temperature and heating time (80, 100 and 1210C for 10, 20 and 30 minute) on the antibacterial effectivity of ethyl acetate and ethanol kecombrang extract were analysed. Both ethyl acetate and ethanol extracts showed antibacterial activity at pH 4-8, but its activity gradually decreased at higher pH. At pH 9, only ethanol extract still showed antibacterial activity. Addition of 1-4% NaCl on ethyl acetate and ethanol extract still showed antibacterial activity. Heating the extracts at 80-100 0C for 10-30 minutes and 1210C for 10 minutes did not haves significantly affect on the antibacterial activity of both ethyl acetate and ethanol extracts. Application of ethyl acetate extract at the concentration of 1 and 3 MIC on minced meat were still effective to reduce the viable bacteria until 7 days and 5 MIC was still effective until 9 days. Key words : Kecombrang, pH, NaCl, temperature and heating time, antibacteria

Study on the Effect of the Use of Bifidobacteria on Flavor of Yoghurt Suryono .; Adi Sudono; Mirnawati Sudarwanto; Anton Apriyantono
Jurnal Teknologi dan Industri Pangan Vol. 16 No. 1 (2005): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.353 KB)

This experiment was carried out to study the effect of bifidobacteria on flavor of yoghurt. Parameters measured in the experiment were acidity, pH, viscosity, volatile composition, sensory acceptance and intensity of yoghurt sensory attributes. Results of the experiment indicated that the use of bifidobacteria in mixture of yoghurt culture was able to increase the levels of acidity and viscosity of yoghurt. The highest acidity and viscosity was found in yoghurt prepared by Lactobacillus bulgaricus and bifidobacteria mixture, and also by Lactobacillus bulgaricus, Streptococcus thermophilus and bifidobacteria mixture cultures. The major classes of volatile component identified were acids, alcohols, ketones and aldehydes. The major component identified were octanoic acid, acetoin and octadecanal. It was found that there was no significant difference in sensory acceptance of the panelist for colour, aroma and taste of yoghurt prepared by the various combination of cultures. However, consistency of yoghurt prepared by S. thermophilus with or without addition of bifidobacteria, was less compared to that of others. Yoghurt prepared by single culture of S. thermophilus showed higher intensity of bitter and syneresis. The use of bifidobacteria in the cultures mixture decrease the intensity of bitter and syneresis of the yoghurt. Keywords : Bifidobacteria, L. bulgaricus, S. thermophilus, flavor of yoghurt

Milkchecker, As An Alternative Tool To Detect Subclinical Mastitis Mirnawati Sudarwanto
Media Veteriner Vol. 4 No. 1 (1997): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.504 KB)

Two hundred and thirty milk samples of different quarters taken from dairy cows around Bogor and Cipanas were used in this study. The milk samples were tested aseptically against subclinical mastitis using the MC and AMP in the field and in the laboratory. The amount of leucocytes were counted using the BREED method.Using the MC the sensitivity is 85.2% and the specificity is 97.8% in detecting subclinical mastitis. In comparing the use of MC to the AMP or to the BREED method no significant difference was found. The MC should be used routinely to detect mastitis especiallynew for cases of subclinical mastitis.

The Application of Decomposting Microbes For Reduce Amonia and Hydrogen Sulfide Emission From The Piggeries in Bali Ni Wajan Leestyawati palgunadi; Mirnawati Sudarwanto; Ida Bagus Arka; Eko Sugeng Pribadi
Media Veteriner Vol. 6 No. 1 (1999): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.644 KB)

The unpleasant odor of piggeries is mostly caused by the emission of ammonia and hydrogen sulfide. An experimentwas conducted to study the effect of decomposting microbes EM4 on the emission of ammonia and hydrogen sulfide. The manure was treated with four different concentrations of EM4@, 0'70, 0.5%, 1.0% and 1.5% respectively. The ammonia released was trapped in 0.02N boric acid and analyzed with Nessler method, while the hydrogen sulfide was trapped in 0.02N zinc acetate and analyzed with the methylene blue method. The study suggested that the high concentrationof ammonia and hydrogen sulfide gas were decreasedafter 14 days, and there was no significant differencesin either ammonia or hydrogen sulfide emission although theammonia emission decreased to under 20 ppm with of 1.5%EM^" and the hydrogen sulfide decreased to 0.202 ppmwith application of 1.0% EM4@.

The IPB-I Reagent As An Alternative Tool To Detect Subclinical Mastitis Mirnawati Sudarwanto
Media Veteriner Vol. 5 No. 1 (1998): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.546 KB)

Mastitis subklinis hanya dapat dideteksi melalui pemeriksaan mikrobiologi dan penghitungan jumlah sel radang terhadap contoh susu. Penyakit ini sangat merugikan peternak karena produksi susu menurun dan seringkali berkembang menjadi mastitis klinik atau kronis yang berakibat penyingkiran sapi lebih awal. Melakukan deteksi dini dengan pereaksi terhadapcontoh susu dapat memperkecil resiko tersebut. Ujimastitis subklinis di lapang yang ada sampai saat ini,seperti Calijbrnia Mastitis Test (CMT), masih jarangdilakukan karena harga pereaksinya cukup lnahal dansulit diperoleh di pasaran. Untuk memperoleh suatuteknik yang cepat dan pereaksi untuk uji mastitis subklinis di lapang yang relatif lebih mudah, murah dan bahan-bahannya mudah diperoleh di pasaran, maka dikembangkanlah pereaksi yang diberi nama "IPB- I ". Dari penelitian ini diperoleh data bahwa sensitivitas IPB- I, CMT, Whiteside Test (WST), Aulendorfer Mastitis Probe (AMP) mod- 1 dan AMP mod-2 berturut-turut 0,99; 0,92; 0,94; 0,92 dan 0,94. Sedangkanspesifisitasnya berturut-turut 0,92; 0,37; 0,32; 0,47 dan0,89. Nilai prediksi (predictive valz~e)I PB- I, CMT, WST, AMP mod-1 dan AMP mod-2 berturut-turut 0,95; 0,99; 0,97; 0,99 dan 0,97. Nilai Keterhandalan IPB-I lebih tinggi dibandingkan dengan pereaksi lainnya. Namun nilai prediksi untuk pereaksi masih harus diperbaiki.

Antimicrobial activity of pliek u oil and pliek u crude extracts were evaluated against seven bacterial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella Enteritidis, Bacillus cereus, Pseudomonas aeruginosa, Pseudomonas fluorescens) and one fungal strain (Candida albicans). Pliek u oil has been used as cooking oil and medicinal of skin diseases, wound, fever, headache and stomache. Pliek u has been consumed as spices and sambal, and also used for feed poultry. Th Nurliana .; Mirnawati Sudarwanto; L.I. Sudirman; Anastasia Winny Sanjaya
Forum Pasca Sarjana Vol. 32 No. 1 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Antimicrobial activity of pliek u oil and pliek u crude extracts were evaluated against seven bacterial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella Enteritidis, Bacillus cereus, Pseudomonas aeruginosa, Pseudomonas fluorescens) and one fungal strain (Candida albicans). Pliek u oil has been used as cooking oil and medicinal of skin diseases, wound, fever, headache and stomache. Pliek u has been consumed as spices and sambal, and also used for feed poultry. These foods collected from home industry at Redeup village in Aceh Besar, Nanggroe Aceh Darussalam. The hexane and ethanol extracts of pliek u were obtained by standard method. The antimicrobial activity was detected using paper disc method and the concentration of crude extract was determined by dilution method. Among antimicrobials extracts tested, the ethanol crude extract (EEP) was most active against all microbial strains. The ethanol crude extract obtained from pliek u previously extracted by hexane (EERH) was active toward bacterial strains and only hexane crude extract was active against C. albicans. The results indicated that ethanol crude extract (EEP) showed antimicrobial activity at a Minimal Inhibitory Concentration (MIC) and a Minimal Microbicidal Concentration (MMC) at 2.5-10 mg/ml and 10-20 mg/ml, respectively. EEP was still active at 100ºC, 121ºC for 15-60 minute, 28ºC (room storage), 10ºC (refrigerator temperature), both for 1-6 months and at pH from 1-11. The results of the research concluded that EEP showed significant antimicrobial activity. Key words: pliek u, coconut fermentated, antimicrobial activity, toxicity test

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants. Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover national demand. In the future it will be a potential problem to national dairy he Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Rochman Naim; Ewald Usleber
Forum Pasca Sarjana Vol. 32 No. 2 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants. Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover national demand. In the future it will be a potential problem to national dairy herd and human health. The aim of this study is to detect MAP in the growing up milk formula. Fifty samples from five established distributors were taken in Bogor. Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold’s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer. Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57. Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia. Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR

Through a nutritional improvement project, Bogor District had donated powdered milk formula (PMF) for severe malnutrition children under five years. Powdered milk formula was assumed as an unsterilized product, because it might contain spore forming bacteria. To evaluate the bacterial contaminant of home prepared milk formula, fourty eight samples of PMF, and 50 samples of reconstitution formula, drinking water, drinking equipment, and hand were taken proporsionally from the mothers in 10 Heal Maya Purwanti; Mirnawati Sudarwanto; Winiati P. Rahayu; A. Winny Sanjaya
Forum Pasca Sarjana Vol. 31 No. 4 (2008): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Through a nutritional improvement project, Bogor District had donated powdered milk formula (PMF) for severe malnutrition children under five years. Powdered milk formula was assumed as an unsterilized product, because it might contain spore forming bacteria. To evaluate the bacterial contaminant of home prepared milk formula, fourty eight samples of PMF, and 50 samples of reconstitution formula, drinking water, drinking equipment, and hand were taken proporsionally from the mothers in 10 Health Community Centre around Bogor District. Samples were analyzed for aerobic microbe, Bacillus cereus, Clostridium perfringens, and the potential of enterotoxins production. All of the donated PMF samples exhibiting a total aerobic count of <104 CFU/g (mean 1.2 x 102 CFU/g) and B. cereus count of <103 CFU/g (mean 3.0 x 102 CFU/g for PMF containing this bacterium). Improper home storage promote the growth of aerobic microbe and B. cereus. Improper preparation promote the growth of B. cereus which present initially at low level (mean 7.8 x 10 CFU/g) and became 1.6 x 103 CFU/ml after the reconstitution. Clostridium perfringens emerge at 4 samples (mean 1.5 x 10 CFU/ml) after the reconstitution. Several isolate of B. cereus (4 from PMF, 13 from opened PMF, and 2 from reconstution formula) showed a possibility to produce diarrheagenic enterotoxin while the isolate of C. perfringens did not shown it. Keywords: powdered milk formula, B. cereus, C. perfringens, diarrheagenic enterotoxin, preparation and storage

In recent years, there has been an increasing an abuse of slaughtered death chicken for human consumption, so it is important to find a practice ways to identify it. Experiments were conducted to determine whether the quality of meat taken from slaughtered death chicken can be detected through quality attributes of meat (Warner-Bratzler (WB) shear value, CIE L* a* b* color, and histological changes. Thirty pieces of breast (M. pectoralis) and thigh (M. biceps femoris) meat were obtained from co Razali .; Denny W. Lukman; Srihadi Agungpriyono; Mirnawati Sudarwanto
Forum Pasca Sarjana Vol. 30 No. 1 (2007): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

In recent years, there has been an increasing an abuse of slaughtered death chicken for human consumption, so it is important to find a practice ways to identify it. Experiments were conducted to determine whether the quality of meat taken from slaughtered death chicken can be detected through quality attributes of meat (Warner-Bratzler (WB) shear value, CIE L* a* b* color, and histological changes. Thirty pieces of breast (M. pectoralis) and thigh (M. biceps femoris) meat were obtained from commercial slaughtering house. The samples were classified into three groups namely halal slaughtered chicken (AHS), slaughtered death chicken (AMS), and slaughtered stressed chicken (ALS) and all samples processed for microscopic observations, for WB value and color after 1, 5 and 9 h postmortem (PM). This study showed that percentage of degenerated and necrotic muscle fibres of breast and thigh meat of AMS and ALS were significantly higher (P<0.05) than AHS. The WB values of all samples were nearly similar (P>0.05). The L* value of breast and thigh meat of AMS and ALS were lower whereas a* value of breast and thigh meat of AMS were significantly (P<0.05) higher than those of AHS and ALS. The muscle fiber interstitial space had significant positive correlation with degenerated muscle fiber (P<0.001, r=0.52), necrotic muscle fiber (P<0.001, r=0.57) of breast meat, and also with degenerated muscle fiber (P<0.001, r=0.68) and necrotic muscle fiber (P<0.001, r=0.56) of thigh meat. The biologic parameters can be used to distinguish between the slaughtered death chicken and halal slaughtered chicken. Key words: meat quality, muscle fiber, slaughtered death chicken, L* a* b* value

Title

Found 27 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 27 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 27 Documents
Search