L K Darusman
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Pemanfaatan Bahan Bio-anorganik untuk Memproduksi Biomassa Hijauan Pakan dan Inokulan Fungi Mikoriza Arbuskula A D Nusantara; C Kusmana; I Mansur; L K Darusman; Soedarmadi Soedarmadi
Media Peternakan Vol. 33 No. 3 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.632 KB) | DOI: 10.5398/medpet.2010.33.3.162

Abstract

Inoculant production of arbuscular mycorrhizal fungi (AMF) has a vital role in sustaining of higher forage legume production. Currently, it is important to find the alternative materials that can be used as sources of phosphorus due to the need of lower cost, environmentaly friendly, and easily available. Bone meal and rock phosphate are some of the bio-inorganic sources that can be used as a phosphorus source for producing biomass of forage legume and AMF's inoculant production. A glasshouse experiment was conducted to determine the effectiveness of different type of bio-inorganic materials as a fertilizer to increase forage legume biomass and for specific AMF's inoculant production. The experiment was arranged in a factorial randomized block design with two factors, i.e. type of AMF's species (Glomus etunicatum NPI-126 and Acaulospora tuberculata INDO-2) and type of bio-inorganic materials (artifical fertilizer solution as a control, SP36, rock phosphate, and bone meal).  Results showed that G. etunicatum gave better result in terms of host gowth, root colonization and spore production compare to A. tuberculata. Bone meal was better a phosphorus source for AMF's inoculant production instead of artifical fertilizer or other bio-anorganic sources. In conclusion, milled bone meal has a good phosphorus source for AMF's (G. etunicatum) inoculant production
Efektivitas Anticestoda Ekstrak Daun Miana (Coleus blumei Bent) terhadap Cacing Hymenolepis microstoma pada Mencit Y Ridwan; F Satrija; L K Darusman; F Handharyani
Media Peternakan Vol. 33 No. 1 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.472 KB)

Abstract

Coleus blumei is a herbal plant used in the traditional medicine in Indonesia to expel the intestinal worm infections. Previous in vitro study showed that ethanol extract had the strongest anticestode activity compared to chloroform, hexane and aquaous extracts. The aim of the study was to evaluate the effectivity of anticestode of ethanol leaves extract againts Hymenolepis microstoma infections in mice. The plant extract was tested against H. microstoma infections in the single doses of 250, 500, 1000, and 2000 mg kg-1 body weight. Dose were administered to H. microstoma infected mice for 3 consecutive days. The efficacy of the leave extract was determined in terms of eggs per gram of feces (EPG) and worms reduction at necropsy. The results showed that the efficacy of leaves extract was dose dependent. The maximum efficacy of leave extract was observed with 2000 mg/kg dose reducing the EPG and worm counts by 55.46%-69.75% and  63.83% respectively.  The standard anticestodal drug, praziquantel at 25 mg/kg single dose revealed 100% reduction in both of EPG and worm counts. The study suggests that the leaves extract of C. blumei possesses significant anticestodal efficacy and supports its use in traditional medicine. Key words: anticestode, Coleus blumei extract, Hymenolepis microstoma
Performa Fungi Mikoriza Arbuskula dan Pueraria phaseoloides yang Dipupuk Tepung Tulang dengan Ukuran dan Dosis Berbeda A D Nusantara; C Kusmana; I Mansur; L K Darusman; . Soedarmadi
Media Peternakan Vol. 34 No. 2 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.226 KB) | DOI: 10.5398/medpet.2011.34.2.126

Abstract

Bone meal is a natural source of calcium and phosphorus required for plant growth, but phosphorus can cause problems if they occur in high concentrations particularly will inhibit growth of mycorrhizal fungi. The aim of this study was to find the best diameter size and dosage of bone meal for increasing growth of forage legume Pueraria phaseoloides and for producing arbuscular mycorrhizal fungi (AMF) Glomus etunicatum inoculant. A glass house experiment was laid out in a randomized block design consisted of 13 treatments (control and 12 combination of different diameter size and weight of bone meal) and replicated three times. Bone meal and artificial fertilizer resulted the same effect (P>0.05) on dry matter of P. phaseoloides. Application of 25–40 mg with <250 µm diameter significantly (P<0.05) increased dry matter production. Bone meal reduced significantly (P<0.05) root colonization but increased significantly number of spores compared to that of artificial fertilizer. Reducing bone meal diameter significantly increased root colonization and number of spores of AMF G. etunicatum.
Optimasi simbiosis cendawan mikoriza arbuskula Acaulospora tuberculata dan Gigaspora margarita pada bibit kelapa sawit di tanah masam Optimizing arbuscular mycorrhizal fungi symbiosis Acaulospora tuberculata and Gigaspora margarita with oil palm seedling in acid soil) Happy WIDIASTUTI; Edi GUHARDJA; Nampiah SOEKARNO; L K DARUSMAN; Didiek Hadjar GOENADI; Sally SMITH
E-Journal Menara Perkebunan Vol 70, No 2: Desember 2002
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.358 KB) | DOI: 10.22302/iribb.jur.mp.v70i2.128

Abstract

SummaryAM fungal symbiosis increase the uptake of P in oil palm seedlings. However the optimum condition of symbiosis has to be determined to get higher benefit of AM fungal symbiosis. Optimization of the symbiosis Acaulospora tuberculata and Gigaspora margarita with oil palm seedling in acid soil was determined. An experiment was conducted in polybag sized 40 x 60 cm contained sterilized Cikopomayak soil. Three factors studied were AM fungal species (A. tuberculata, G. margarita), inoculant dose (0.0; 12.5; 25.0; and 37.5% w/w), and fertilizer rate (0; 25; 50; and 100% recommended dose) and each treatment replicated three times. The result showed that optimum growth reached on the inoculant addition of 36% (w/w) in the form of infected roots, hypha, and spores and fertilizer dose of 25% for A. tuberculata, while for G. margarita was 40% (w/w) inoculant and 26% fertilizer. Efectivity of fertilizer and P uptake of oil palm seedling were significantly increased with AM fungi inoculation. P uptake of oil palm seedling inoculated with A. tuberculata increase. RingkasanSimbiosis cendawan mikoriza arbuskula (CMA) dapat meningkatkan serapan P pada pembibitan kelapa sawit. Namun, untuk mendapatkan keuntungan simbiosis yang tinggi perlu diketahui kondisi optimum simbiosis. Simbiosis CMA dengan tanaman sangat dipengaruhi tingkat hara dan dosis inokulum. Percobaan dilakukan dalam polibag berukuran 40 x 60 cm berisi tanah Cikopomayak steril. Tiga faktor yang diuji ialah spesies CMA (A. tuberculata, G. margarita), dosis inokulum campuran (0,0; 12,5; 25,0; dan 37,5% b/b), dosis pupuk (0; 25; 50; dan 100% dosis rekomendasi) dan masing masing perlakuan diulang tiga kali. Hasil percobaan menunjukkan bahwa pertumbuhan optimum dicapai pada pemberian inokulum berupa akar terinfeksi, hifa, dan spora 36% (b/b) dan pupuk 25% untuk A. tuberculata, sedangkan untuk G. margarita ialah 40% (b/b) inokulum dan pupuk 26%. Keefektifan pupuk dan serapan P meningkat secara nyata dengan inokulasi CMA