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Kadar Sianida Singkong Rebus dan Singkong Goreng Yeni Purwati; Anny Thuraidah; Dinna Rakhmina
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (829.559 KB) | DOI: 10.31964/mltj.v2i2.93

Abstract

Cassava is the third staple food often used by people to make a variety of products. Carbohydrates from cassava also contains toxic compound such as cyanide which can inhibit the action of respiratory enzymes. Cyanide in cassava can be removed by boiling or frying process. The aim of research was to find out the cyanide level differences between boiled cassava and fried cassava. This type of research was experiment, with design of comparative studies approach. Cyanide levels were examined in 10 samples with 2 treatments using Argentometry Volhard titration method. The result showed the average of cyanide levels in boiled cassava was 0.772 ppm and in fried cassava was 1.069 ppm. The percentage of cyanide levels reduction in boiled cassava was 28,78%, while for the fried cassava was 0%. The statistical test obtained the value of p
Pengaruh Genjer (Limnocharis flava) terhadap Penurunan Biological Oxygen Demand (BOD) Limbah Industri Karet Anny Thuraidah; Erie Indra Puspita; Neny Oktiyani
Medical Laboratory Technology Journal Vol. 2 No. 1 (2016): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.547 KB) | DOI: 10.31964/mltj.v2i1.28

Abstract

Abstract: The levels of Biological Oxygen Demand (BOD) in the waste can be reduced by utilizing water plants such as velvetleaf (Limnocharis flava) as phytoremediation. This study aims to determine the influence of the many clumps Limnocharis flava to decreased levels of effluent BOD rubber industry with a variety clump Limnocharis flava much as 1, 2, 3, 4, and 5 clumps and clumps Limnocharis flava know the number of the most influential in lowering levels of BOD. This type of research is true experimental design with one group pretest-posttest. The research material was Limnocharis flava. The result showed the levels of initial BOD rubber waste amounted to 299.5 mg / l, decreased levels of BOD of waste rubber after being treated with Limnocharis flava with a variety of clumps. Decreased levels of BOD rubber waste for each successive treatment ranging from 1, 2, 3, 4, and 5 clumps genjer ie 13.65%, 14, 52%, 15.6%, 20.94%, and 29.05%. Based on test results obtained linear regression for each treatment their effect on levels of BOD using genjer rubber waste. Treatment using Limnocharis flava much as 5 clump results BOD levels decrease the total of up to 29.05%. This value has not met the standard the maximum allowable levels of BOD. Need soaking clumps Limnocharis flava more stout and longer so that the rubber waste reduction in BOD levels in accordance with the quality standards required.
Effectiveness of Cinnamon (Cinnamomum burmannii) Ethanol Extract Against Staphylococcus aureus Growth Nurul Huda; Ratih Dewi Dwiyanti; Anny Thuraidah
Tropical Health and Medical Research Vol. 1 No. 2 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.614 KB) | DOI: 10.35916/thmr.v1i2.6

Abstract

Cinnamon (Cinnamomum burmannii) is widely used by the public as a food ingredient and contains chemical compounds such as alkaloids, flavonoids, polyphenols, saponins, and terpenoids which function as an antibacterial against Staphylococcus aureus. This study aims to determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of cinnamon ethanol extract on Staphyloccocus aureus. This research is experimental with posttest only control group design through the tube dilution method. The results of the Minimum Inhibitory Concentration (MIC) study showed no clarity at concentrations of 30% and 40%. The results of the Minimum Bactericidal Concentration (MBC) obtained the number of colonies at a level of 10% by 51 CFU / plate, 20% by 27 CFU / plate, 30% by 6 CFU / plate and 40% by 0 CFU / plate. Based on the results of this study concluded that MIC cinnamon ethanol extract was 30%, and MBC cinnamon ethanol extract was 40%.
Citrus hystrix D.C Juice Inhibits The Growth of Staphylococcus aureus Novina Kusumawardhani; Anny Thuraidah; Nurlailah Nurlailah
Tropical Health and Medical Research Vol. 2 No. 1 (2020): Tropical Health And Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.587 KB) | DOI: 10.35916/thmr.v0i0.17

Abstract

Citrus hystrix D.C. is a citrus-type citrus tribal plant, one of the most favored and developed fruits by the people of South Kalimantan Indonesia. Citrus hystrix D.C. contains useful chemical compounds, one of which is flavonoids, which function as antibacterial and play a critical role in inhibiting bacterial growth. The purpose of this study was to determine the antibacterial activity of Citrus hystrix D.C. juice at 25%, 50%, 75%, and 100% concentration on the growth of Staphylococcus aureus. This research is experimental with Posttest Only Control Group Design. The material used was local Citrus hystrix D.C., fresh and clean green, obtained from the Astambul area, Banjar Regency, South Kalimantan Indonesia. The results showed that there was a zone of inhibition of Citrus hystrix D.C. juice on Staphylococcus aureus growth which began at 25%, 50%, 75% and 100% concentrations of 13.75mm, 17.25mm, 19.75mm, and 21.75mm, so it can conclude that the juice of Citrus hystrix D.C. influences the growth of Staphylococcus aureus which has the largest inhibitory zone at a concentration of 100%.
Garlic Extract (Allium sativum L.) Effectively Inhibits Staphylococcus aureus and Escherichia coli by Invitro Test Agnina Listya Anggraini; Ratih Dewi Dwiyanti; Anny Thuraidah
Tropical Health and Medical Research Vol. 2 No. 2 (2020): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.775 KB) | DOI: 10.35916/thmr.v0i0.22

Abstract

Infection is a disease caused by the presence of pathogenic microbes, including Staphylococcus aureus and Escherichia coli. Garlic (Allium sativum L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against Staphylococcus aureus and Escherichia coli. This study aims to determine the antibacterial properties of garlic extract powder against Staphylococcus aureus and Escherichia coli. This research is the initial stage of the development of herbal medicines to treat Staphylococcus aureus and Escherichia coli infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus and Escherichia coli was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for Staphylococcus aureus and Escherichia coli are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of Staphylococcus aureus and Escherichia coli is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of Staphylococcus aureus and Escherichia coli by 54.5% and 78.5%. Garlic (Allium sativum L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.
Fungicidal Effects of Chloroform Extract of Red Galangal (Alpinia purpurata (Vieill.) K. Sch) on the Growth of Trichophyton rubrum Nur Asy Syifa Azizah; Anny Thuraidah; Ratih Dewi Dwiyanti
Tropical Health and Medical Research Vol. 4 No. 1 (2022): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v4i1.59

Abstract

Trichophyton rubrum is the most common causative agent of anthropophilic dermatophytosis worldwide. Treatment of dermatophytosis can use natural ingredients. In Indonesia, several medicinal plants have been used, one of which is red galangal (Alpinia purpurata (Vieill.) K. Sch). This study aims to determine the effectiveness of red galangal rhizome chloroform extract on the growth of Trichophyton rubrum. This study is a True Experiment study with Posttest Only With Control Group Design consisting of 8 treatments namely positive control, extract control, 0 mg/mL (negative control), 20 mg/mL, 30 mg/mL, 40 mg/mL, 50 mg/ml, and 60 mg/mL extract of red galangal rhizome chloroform with 3 repetitions. The antifungal activity test was carried out using the determination of Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), and Bioautography Test. The results showed that the red galangal rhizome chloroform extract in the Agar Overlay Bioautography Test contained an inhibition zone on the growth of Trichophyton rubrum. The determination of MIC and MFC were 20 mg/mL and 40 mg/mL, respectively. The study concludes that the red galangal rhizome chloroform extract affects the growth of the fungus Trichophyton rubrum. Research can be continued by knowing more specifics about the bioactive compounds from the red galangal rhizome, which have antifungal activity against Trichophyton rubrum.
Bactericidal Potential of Extract Citrus Hystrix D.C.Leaf Powder on Staphylococcus aureus and Salmonella typhi Elfira Maulidah; Anny Thuraidah; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 12 No 1 (2021): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jsk.v11i2.279

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Infectious diseases can be caused by bacteria such as Staphylococcus aureus and Salmonella typhi. Treatment of infectious diseases can use antibiotics. However, antibiotics can cause side effects on users. Kuit Lime leaf (Citrus hystrix D.C.) contains secondary metabolites such as alkaloids, steroids, triterpenoids, tannins, and flavonoids used as antibacterial. This study aimed to determine the bactericidal potential of the kuit lime leaf extract powder on Staphylococcus aureus and Salmonella typhi. The type of research used is the experiment, and the research design used is the Posttest Only Control Group Design. The antibacterial activity test used was the liquid dilution of MIC and MBC. The results showed the MIC of Staphylococcus aureus and Salmonella typhi was 180 mg/mL and 120 mg/mL, while the MBC results against Staphylococcus aureus and Salmonella typhi are 190 mg/mL and 130 mg/mL. Data analysis in this research conducted descriptively. Based on descriptive analysis, it concluded that the bactericidal potential of the powdered extract of kuit lime against Staphylococcus aureus is at a concentration of 190 mg/mL and Salmonella typhi at a concentration of 130 mg/mL.
Phytochemical Analysis by LC-HRMS and Antibacterial Activity Of the Ethanol Extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) Ratih Dewi Dwiyanti; Anny Thuraidah; Nurlailah Nurlailah
Medical Laboratory Technology Journal Vol. 9 No. 1 (2023): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v9i1.506

Abstract

Dracontomelon dao (Blanco) Merr. & Rofe is one of the medicinal plants for the community. This study aims to analyze the ethanol extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) using the LC-HRMS Phytochemical Test and determine its antibacterial activity. Leaf Dracontomelon dao (Blanco) Merr. & Rofe originates from Hulu Sungai Utara, South Kalimantan, Indonesia, macerated with 96% ethanol. Antibacterial test by diffusion was carried out using the well method. LC-HRMS (Liquid Chromatography - High-Resolution Mass Spectrometry) test using the Instrument Thermo Scientific™ Dionex™ Ultimate 3000 RSLCnano UHPLC coupled with Thermo Scientific™ Q Exactive™ High-Resolution Mass Spectrometer. The content of chemical compounds from the ethanol extract, namely flavonoids 125.5 ± 0.433 mgEQ/g, alkaloids (%) 33.945 ± 0.781, saponins (%) 33.093 ± 0.755, tannins (mg/ml) 0.069 ± 0.003, the highest content was triterpenoids 669.8 ± 2,000(mg/ml). The best inhibition of E coli growth with the largest inhibition zone was 24 mm at a 500 mg/ml concentration. The active compounds contained in the leaves of Dracontomelon dao (Blanco) Merr. & Rolfe are quercetin-3β-D-glucoside, anacardic acid, D-glucosamine, azelaic acid, choline, astragalin, quercetin, luteolin, syringic acid. The active ingredient of the ethanol extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) has the potential as an antibacterial and anti-inflammatory that can be added to pharmaceutical preparations. Keywords: Dracontomelon dao (Blanco) Merr. & Rofe; Phytochemical Analysis; Liquid Chromatography - High-Resolution Mass Spectrometry
Analysis of Urea Levels of Banjarmasin Ministry of Health Polytechnic Students Survivors of Covid 19 Siti Nur Shalehah Hasanah; Anny Thuraidah; Haitami Haitami
Tropical Health and Medical Research Vol. 5 No. 2 (2023): Tropical Health And Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v5i1.97

Abstract

Corona Virus Disease 2019 (Covid-19) is an infection caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Drug therapy for Covid-19 patients currently uses broad-spectrum antivirals such as Remdesivir, Favipiravir, and Oseltamivir, which have side effects of impaired kidney function with a marked increase in serum urea levels. The study aims to analyze the relationship between antiviral administration and serum urea levels of Covid-19 survivors with student respondents from the Health Polytechnic of the Ministry of Health, Banjarmasin, Indonesia. The research method is an analytic survey with a case-control design. Samples were taken using the purposive sampling method and obtained from 10 respondents who were confirmed positive for Covid-19 who consumed antivirals, ten respondents who were confirmed positive for Covid-19 did not consume antivirals, and ten respondents who were not confirmed positive for Covid-19. The Urea test method uses the Rayto Chemray 120 Clinical Analyzer. The results showed that the average urea level of respondents who confirmed positive for Covid-19 who consumed antivirals was 37.35 mg/dl, and respondents who confirmed positive for Covid-19 who did not consume antivirals was 30.70 mg/dl. Respondents who were not confirmed for Covid-19 19 are 30.97 mg/dl. The study's conclusion showed a relationship between the administration of antivirals and serum urea levels of Covid-19 survivors. Suggestions for further research are to use different parameters in assessing the kidney function of Covid 19 survivors, such as creatinine and blood urea nitrogen.