Claim Missing Document
Check
Articles

Found 8 Documents
Search

Kadar Sianida Singkong Rebus dan Singkong Goreng Yeni Purwati; Anny Thuraidah; Dinna Rakhmina
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (829.559 KB) | DOI: 10.31964/mltj.v2i2.93

Abstract

Cassava is the third staple food often used by people to make a variety of products. Carbohydrates from cassava also contains toxic compound such as cyanide which can inhibit the action of respiratory enzymes. Cyanide in cassava can be removed by boiling or frying process. The aim of research was to find out the cyanide level differences between boiled cassava and fried cassava. This type of research was experiment, with design of comparative studies approach. Cyanide levels were examined in 10 samples with 2 treatments using Argentometry Volhard titration method. The result showed the average of cyanide levels in boiled cassava was 0.772 ppm and in fried cassava was 1.069 ppm. The percentage of cyanide levels reduction in boiled cassava was 28,78%, while for the fried cassava was 0%. The statistical test obtained the value of p
Kandungan Asam Oksalat Sayur Bayam Herlena Fitriani; Nurlailah Nurlailah; Dinna Rakhmina
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (673.028 KB) | DOI: 10.31964/mltj.v2i2.95

Abstract

Spinach is one of vegetable that is often used as processed food by the people of Indonesia. Besides containing many nutrients, spinach also contains chemical compounds that are negative, that is oxalic acid. Oxalic acid and its salts are water soluble that can be harmful because these compounds are toxic. This study aimed to determine differences in levels of oxalic acid in spinach when the water is allowed to stand at room temperature. The type of research was pre-experiment with one group pretest-posttest design. Samples of spinach water were divided into 4 treatment and the level of oxalic acid was examined by using permanganometry titration method. Results of research on each treatment showed oxalic acid levels on 0 hour standing was 3753.2 mg/L, 2 hours standing was 3980.0 mg/L, 4 hours standing was 4066.5 mg/L, and the 6 hours standing was 4254.5 mg/L. Repeated ANOVA statistical test results stated there were significant differences in the levels of oxalic acid in spinach water between 0 hour standing and room temperature-standing with a significance value of p
Effect of Active Carbon of Coffee Robusta Waste (Coffea robusta Lindl.) in Reducing Iron of Peat Water Dinna Rakhmina; Rizki Mulanova; Haitami Haitami
Medical Laboratory Technology Journal Vol. 4 No. 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (966.956 KB) | DOI: 10.31964/mltj.v4i1.173

Abstract

Peat water has a distinctive dark brown or yellow color, low pH, it tastes sour, high organic matter content, high levels of iron and manganese. One of the ways that can be used to reduce the levels of iron in peat water is doing adsorption process by using activated carbon coffee waste because the coffee waste included organic ingredients. The purpose of this research was to identify the influence of the concentration of activated carbon robusta coffee waste (Coffea robusta Lindl.) against the iron levels in peat water. This research uses the draft Posttest-Only Control Group Design. Manufacture of activated carbon robusta coffee waste (Coffea robusta Lindl.) consists of the processes of dehydration, carbonization, and activation, with the results 25.9% of rendemen, 14.05% water content, a rate of 9.9% ash, and iod absorbance 647.19 mg/g. Activated carbon coffee waste added to 100 ml of peat water as much as 3 gr, 4.5 gr, and 6 gr with the stirring speed 400 rpm for 60 minutes obtained early iron levels of 2.75 mg/L and dropped to 1.19 mg/L, 1.02 mg/L, and 0.95 mg/L. Conclusions This study is there is the influence of the concentration of activated carbon robusta coffee waste (Coffea robusta Lindl.) against the iron level of peat water iron and frequency of 6 gr as a concentration of the best efficiency adsorption of 65.4% because it can reduce the levels of iron in 100 ml of peat water from 2.75 mg/L be 0.95 mg/L. By regulation of the Minister of health RI No. 416/Menkes/PER/IX/1990 namely of 1.0 mg/L. Suggestions for further research to improve the heating temperature and concentration of the Activator to obtain active carbon absorption.
MiR-21 and mRNA PTEN Expression Levels and Biomarker Potential in Breast Cancer Dinna Rakhmina; Sofia Mubarika Haryana; Teguh Aryandono
Medical Laboratory Technology Journal Vol. 7 No. 1 (2021): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (802.167 KB) | DOI: 10.31964/mltj.v7i1.364

Abstract

MiR-21 has been linked to tumorigenesis, development, and metastasis in tumor pathogenesis. All human cancers, including breast cancer, have increased expression of MiR-21, which is the only miRNA that has increased expression. PTEN expression was found to be reduced in the majority of solid tumors, including breast cancer. Since lymph node metastatic factors, estrogen receptor status, tumor grade, and tumor node metastasis (TNM) all decreased PTEN expression, the PTEN expression profile may be a very useful prognostic marker in breast cancer. PTEN inhibits PIP3 (phosphatidylinositol 3,4,5-triphosphate) activity by having protein phosphatase and lipid phosphatase activity that is the polar opposite of PI3K (Phosphatidyl Inositol 3-Kinase). The aim of this research was to see how often miR-21 and mRNA PTEN were expressed at different stages of breast cancer and whether they could be used as prognostic markers. This type of research is an observational study with a cross-sectional design. The sample size of 43 people came from breast cancer patients. Analysis of miR-21 expression and mRNA PTEN using Real-Time qPCR. The results showed that miR-21 expression increased 1.32 times at an advanced stage compared to an early stage, while mRNA PTEN expression decreased 1.33 fold at an advanced stage compared to an early stage. According to the findings, miR-21 expression in the blood plasma of breast cancer patients was upregulated at an advanced stage compared to an early stage and downregulated mRNA PTEN expression. MiR-21 which is increased at an advanced stage has the potential to be a poor prognostic marker at the stage of breast cancer. The change in miR-21 expression can be a good candidate as a molecular prognostic marker and for future research the role of miR-21 in breast cancer progression will further enrich the scientific repertoire, especially in the health and clinical fields.
Logam Merkuri pada Masker Pemutih Wajah di Pasar Martapura Dinna Rakhmina; Lisa Lisa; Jasmadi Joko Kartiko
Medical Laboratory Technology Journal Vol. 3 No. 2 (2017): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (667.601 KB) | DOI: 10.31964/mltj.v3i2.172

Abstract

Currently, many brands and types of skin whitening cosmetics can be found on the market. Not all products are safe for consumers. Illegal or non-licensed cosmetics from BPOM are suspect because unlisted cosmetics tend to contain harmful chemicals such as mercury (Hg). Mercury (Hg) was including harmful heavy metals. The use of mercury has been banned since 1998 through PERMENKES RI No. 445 of 1998, where mercury in small concentrations can be toxic. Negative effects of mercury such as allergies, skin irritation and permanent damage to the nervous system, kidney, brain and disrupt the development of the fetus, especially when used in high doses. The aim of this study was to know whether there is mercury content on bleach mask that sold in Martapura market in March 2017. This study used a descriptive method and the sample was taken by purposive sampling technique. There were 10 samples of face bleach mask that cost around Rp5.000,00 - Rp20.000,00. Based on the results of 10 samples taken at Martapura market, 3 samples contained mercury of face bleach mask. The conclusion of this study is that mercury still used as bleach on the facial in the Martapura market. Therefore, BPOM needs more supervision and intensive counseling about the risk of bleaching cosmetics containing mercury, so the consumer can be careful in choosing cosmetics.
Ketepatan Hasil dan Variasi Waktu Pendidihan Pemeriksaan Zat Organik Haitami Haitami; Dinna Rakhmina; Syahid Fakhridani
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.144 KB) | DOI: 10.31964/mltj.v2i2.117

Abstract

The principle of organic substances determination in water samples is oxidized by an excess amount of KMnO4, then it is reduced excess amount of oxalic acid, the excess of oxalic acid is titrated back with KMnO4. Based on the procedures in ISO 06-6989.22-2004, a length of boiling time for organic substances determination in water samples is for 10 minutes. This study aimed to determine the effect of boiling time length to the accuracy of the organic substances determination result in water samples. This study was true experiment study with comparative study design. Samples were organic substances, specifically oxalic acid in distilled water in a concentration of 54 mg/L, which was examined for the organic substances level with a boiling time of 5, 10 and 15 minutes. The result on each boiling time length consecutively were 52,8 mg/L; 54,5 mg/L; dan 55,1 mg/L. The conclusion of the study was there was effect of boiling time length to the accuracy of organic substances determination result in water samples with the significance value of 0.002 (
Efektivitas Ekstrak Etanol Daun Jambu Biji Merah (Psidium guajava L.) Sebagai Larvasida Terhadap Mortalitas Larva Aedes aegypti Nur Rizka Adriana; Rifqoh Rifqoh; Dinna Rakhmina; Ratih Dewi Dwiyanti
Jurnal Labora Medika Vol 6, No 2 (2022): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jlabmed.6.2.2022.36-40

Abstract

ABSTRACT  The vector control of Aedes aegypti mosquito that causes DHF is carried out by chemical control of the larvae. Resistance can occur if used repeatedly over a long time. Another alternative that can be used is with natural ingredients such as red guava leaves because they have secondary metabolites that can be used as larvicides. This study aimed to determine the effectiveness of red guava leaf extract (Psidium guajava L.) as a larvicide on mortality of Aedes aegypti and Lethal Concentration 50 and 90 from ethanol extract of red guava leaves. This type of research is a quasi-experimental and posttest with control group design. The test material used was 25 larvae of Aedes aegypti instar III each treatment and the extract was obtained using the maceration extraction method. The concentration variation is 8%; 9%; 10%; 11%; 12% with 4 repetitions. The results obtained that the average mortality of larvae at a concentration of 8% was 13 tails (51%); 9% 18 tails (72%); 10% 22 tails (88%); 11% 23 tails (92%), 12% 25 tails (100%). The Regression test results showed the effect of adding extract was 77.9% at a 24-hour exposure time. The results of the Probit test for the LC50 value obtained a concentration of 24.0824 ppm and LC90 of 32.0479 ppm. Keywords: Ethanol Extract of Guava Leaves, Aedes aegypti Larvae, Lethal Concentration 50, Lethal Concentration 90.  ABSTRAK Pengendalian vektor nyamuk Aedes aegypti penyebab DBD dilakukan dengan pengendalian larva secara kimiawi. Resistensi dapat terjadi jika digunakan berulang dalam jangka waktu yang panjang. Alternatif lain yang dapat digunakan yaitu dengan bahan alami seperti daun jambu biji merah karena memiliki senyawa metabolit sekunder yang dapat digunakan sebagai larvasida. Tujuan penelitian ini untuk mengetahui efektivitas dari ekstrak daun jambu biji merah (Psidium guajava L.) sebagai larvasida terhadap mortalitas larva Aedes aegypti dan Lethal Concentration 50 dan 90 dari ekstrak etanol daun jambu biji merah. Jenis penelitian ini adalah Quasi eksperiment dan rancangan Posttest With Control Group Design. Bahan uji yang digunakan adalah larva Aedes aegypti instar III sebanyak 25 ekor setiap perlakuan dan ekstrak daun jambu biji merah dengan metode ekstraksi maserasi. Variasi konsentrasi yaitu 8%; 9%; 10%; 11%; 12% dengan 4 kali pengulangan. Hasil penelitian yang didapatkan rata-rata kematian larva pada konsentrasi 8% yaitu 13 ekor (51%); 9% 18 ekor (72%); 10% 22 ekor (88%); 11% 23 ekor (92%), 12% 25 ekor (100%). Hasil uji Regresi menunjukkan pengaruh penambahan ekstrak sebesar 77,9% pada waktu pemaparan 24 jam. Hasil uji Probit nilai LC50 didapatkan konsentrasi sebesar 24,0824 ppm dan LC90 sebesar 32,0479 ppm. Kata Kunci: Ekstrak Etanol Daun Jambu Biji Merah, Larva Aedes aegypti, Lethal Concentration 50, Lethal Concentration 90
Hubungan Konsumsi Harian Rokok Terhadap Antibodi IgG S1RBD Pasca Vaksinasi COVID-19 pada Civitas Akademik Amalia Putri; Wahdah Norsiah; Tini Elyn Herlina; H. Haitami; Dinna Rakhmina
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.29

Abstract

Vaksinasi dinilai sebagai cara paling efektif untuk penanganan pandemi akibat COVID-19. Vaksinasi memiliki efek yang berbeda pada tiap individu. Kandungan nikotin dalam rokok diduga menghambat aktivasi kekebalan bawaan dan adaptif, sehingga mempengaruhi pembentukan antibodi yang diinduksi dari vaksinasi.Tujuan penelitian ini untuk mengetahui ada atau tidaknya hubungan konsumsi harian rokok seseorang dengan kadar antibodi IgG S1RBD. Jenis penelitian yang digunakan adalah survei analitik dengan metode cross sectional study pada civitas akademik yang memiliki kebiasaan merokok. Hasil penelitian dari 28 responden didapatkan kadar rata-rata antibodi IgG S1RBD pada responden dengan konsumsi harian rokok 1-10 batang/ hari sebesar 255,59 IU/mL, 11-20 batang/ hari sebesar 173,15 IU/mL, lebih dari 20 batang/hari sebesar 143,79 IU/mL, dan pada non perokok sebesar 279,21 IU/mL. Uji statistik menunjukkan nilai signifikansi 0,000 sehingga disimpulkan semakin tinggi konsumsi harian rokok seseorang maka semakin menurun antibodi IgG S1RBD dalam tubuhnya.