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Nurlailah Nurlailah

Department Of Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin

Author-ID : 2505322

Religion Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Articles

Biolarvasides of Cananga odorata Flower and Zyzygium polyanthum Leaves Ethanol Extract on Aedes aegypti Larva Nurlailah Nurlailah; Anny Thuraidah
Medical Laboratory Technology Journal Vol. 6 No. 1 (2020): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Dengue hemorrhagic fever (DHF) is one of the diseases caused by Aedes Aegypti mosquitoes. DHF is still a public health problem, including in Indonesia, due to the high mortality rate caused by this mosquito. The risk caused by the bite of the Aedes sp mosquito is quite dangerous, including acute fever, bleeding, shock, and even death, so that efforts are needed to control it. One of the natural ingredients that can use to eradicate Aedes Aegypti mosquitoes without causing negative impacts on the environment is the Cananga flower and Zyzigium Leaves. This study purposes of determining the optimum concentration of Cananga Flowers and Zyzigium Leaves Ethanol extract of which was having biolarvaside effect to Aedes Aegypti larvae. The method used a simple experiment with Posttest Only With Control Group Design. The data result of the research analyzed with Logit and Wilcoxon test. Achievement of those tests was LC50 Zyzigium leaf extract on contact for 24 hours was 0.609-0.935, and LC99 was between 1.064-1.973 while Cananga flower extract obtained LC50 of 0.228-0.261 and the LC99 was 0.446 to 0.558. Wilcoxon test for compared bioalarvaside effect results between Cananga Flower and Zyzigium Leaf received a significance value less than α (0.001

Kandungan Asam Oksalat Sayur Bayam Herlena Fitriani; Nurlailah Nurlailah; Dinna Rakhmina
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Spinach is one of vegetable that is often used as processed food by the people of Indonesia. Besides containing many nutrients, spinach also contains chemical compounds that are negative, that is oxalic acid. Oxalic acid and its salts are water soluble that can be harmful because these compounds are toxic. This study aimed to determine differences in levels of oxalic acid in spinach when the water is allowed to stand at room temperature. The type of research was pre-experiment with one group pretest-posttest design. Samples of spinach water were divided into 4 treatment and the level of oxalic acid was examined by using permanganometry titration method. Results of research on each treatment showed oxalic acid levels on 0 hour standing was 3753.2 mg/L, 2 hours standing was 3980.0 mg/L, 4 hours standing was 4066.5 mg/L, and the 6 hours standing was 4254.5 mg/L. Repeated ANOVA statistical test results stated there were significant differences in the levels of oxalic acid in spinach water between 0 hour standing and room temperature-standing with a significance value of p

Utilization of Activated Corn Cob (Zea Mays) as an Improved Adsorbent for Reducing Chemical Oxygen Demand (COD) Value from Waste of the Sasirangan Industry Erfan Roebiakto; Noor Hikmah Damayanti; Neni Oktiyani; Nurlailah Nurlailah
Medical Laboratory Technology Journal Vol. 7 No. 1 (2021): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Liquid waste from sasirangan industrial activities has a high enough Chemical Oxygen Demand pollutant power. If it is directly discharged into water bodies, it can damage the environment and harm health. One of the first processes needs to be done by using activated corn cobs (Zea mays). This study aimed to analyze the ability of corn cobs charcoal to reduce levels of Chemical Oxygen Demand and increase the pH of sasirangan waste so that the results of this study can be an alternative to natural-based sasirangan waste treatment. This type of research is a pure experiment with a research design in One Group Pretest Posttest Design. The research material used was sasirangan industrial waste in Manarap Village, Kertak Hanyar District, Banjar Regency, South Kalimantan, Indonesia. Chemical Oxygen Demand levels were determined by the closed reflux titrimetric method. The results showed that the addition of the highest dose of activated corncob charcoal (50 g) reduced the largest turbidity by 35 percent, increased the pH by 72 percent, and reduced the color intensity by 33 percent. The conclusion is that the addition of corncob-activated charcoal at a dose of 30gr, 40gr, 50gr can reduce levels of Chemical Oxygen Demand, respectively, namely 24 percent, 35 percent, and 33 percent. An increase in pH was found at the same dose of 46 percent, 62 percent, and 72 percent, respectively. There is an effect of increasing the mass of activated charcoal from corn cobs on the Chemical Oxygen Demand levels in the sasirangan industrial waste with a significance value of 0.007. It is suggested to use corn cobs-activated charcoal for the pretreatment stage of sasirangan industrial waste treatment.

Citrus hystrix D.C Juice Inhibits The Growth of Staphylococcus aureus Novina Kusumawardhani; Anny Thuraidah; Nurlailah Nurlailah
Tropical Health and Medical Research Vol. 2 No. 1 (2020): Tropical Health And Medical Research
Publisher : Baiman Bauntung Batuah Center

Citrus hystrix D.C. is a citrus-type citrus tribal plant, one of the most favored and developed fruits by the people of South Kalimantan Indonesia. Citrus hystrix D.C. contains useful chemical compounds, one of which is flavonoids, which function as antibacterial and play a critical role in inhibiting bacterial growth. The purpose of this study was to determine the antibacterial activity of Citrus hystrix D.C. juice at 25%, 50%, 75%, and 100% concentration on the growth of Staphylococcus aureus. This research is experimental with Posttest Only Control Group Design. The material used was local Citrus hystrix D.C., fresh and clean green, obtained from the Astambul area, Banjar Regency, South Kalimantan Indonesia. The results showed that there was a zone of inhibition of Citrus hystrix D.C. juice on Staphylococcus aureus growth which began at 25%, 50%, 75% and 100% concentrations of 13.75mm, 17.25mm, 19.75mm, and 21.75mm, so it can conclude that the juice of Citrus hystrix D.C. influences the growth of Staphylococcus aureus which has the largest inhibitory zone at a concentration of 100%.

Ethanol Extract Of Centella Asiatica (L.) Urban Leaves Effectively Inhibit Streptococcus pyogenes and Pseudomonas aeruginosa by Invitro Test Dini Aulia Azmi; Nurlailah Nurlailah; Ratih Dewi Dwiyanti
Tropical Health and Medical Research Vol. 2 No. 2 (2020): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Streptococcus pyogenes and Pseudomonas aeruginosa are some of the causes of infectious diseases. Centella asiatica (L.) Urban has many benefits for humans, including overcoming fever, anti-bacterial, and anti-inflammatory. This study aims to determine the inhibition of Centella asiatica (L.) Urban leaves ethanol extract on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. This research is the initial stage of the development of herbal medicines to treat Streptococcus pyogenes and Pseudomonas aeruginosa infections. The independent variable was the concentration of ethanol extract of Centella asiatica (L.) Urban leaves and the dependent variable was the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. The anti-bacterial activity test was carried out by the liquid dilution method. The concentrations used are 20%, 40%, 60%, 80%. 100% The results showed that the minimum inhibitory concentration (MIC) against Streptococcus pyogenes: 40% and Pseudomonas aeruginosa: 40%. Minimum bactericidal concentration (MBC) results for Streptococcus pyogenes: 60% and Pseudomonas aeruginosa: 60%. So it can be concluded that there is inhibition of the ethanol extract of Centella asiatica (L.) Urban leaves on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. Centella Asiatica (L.) Urban extract has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

Screening of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) as Antibacterial Escherichia coli Ratih Dewi Dwiyanti; Nurlailah Nurlailah
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Dracontomelon dao (Blanco) Merr. & Rofe has antimicrobial potential, but Dracontomelon dao (Blanco) Merr. & Rofe, originating from Kalimantan, Indonesia, has not been tested for its antibacterial properties and secondary metabolite content. This study aimed to analyze the secondary metabolite content of various Dracontomelon dao (Blanco) Merr leaf extracts. & Rofe and their antibacterial effectiveness against Escherichia coli. The study used 96% ethanol extract, ethyl acetate, petroleum ether, and chloroform by maceration simplicia leaves. Dracontomelon dao (Blanco) Merr. & Rofe is from South Kalimantan, Indonesia. Antibacterial testing was carried out using the agar diffusion method, the Minimum Inhibitory Concentration (MIC) method, and the Minimum Bactericidal Concentration (MBC) method. The results showed that the triterpenoid content was the highest in all extracts, namely ethanol extract 669.8 ± 2,000 mg/ml, ethyl acetate 90 ± 7,638 mg/ml, petroleum ether 792, 800 ± 4,583 mg/ml, chloroform 615, 467 ± 0,577 mg/ml. ml. The antibacterial test using the well diffusion method showed the best inhibition of bacterial growth in ethanol extract, with the largest inhibition zone of 24 mm at a concentration of 500 mg/ml. The study's conclusion was the extract of ethanol, ethyl acetate, petroleum ether and chloroform from the leaves of Dracontomelon dao (Blanco) Merr. & Rofe contains the same five secondary metabolites: flavonoids, alkaloids, saponins, tannins and triterpenoids. All types of extracts showed varying abilities to inhibit the growth of Escherichia coli. Further research is needed on the antibacterial properties of Dracontomelon dao (Blanco) Merr extract. & Rofe, especially on the active substance triterpenoid, which is the largest content in all types of extracts.

Phytochemical Analysis by LC-HRMS and Antibacterial Activity Of the Ethanol Extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) Ratih Dewi Dwiyanti; Anny Thuraidah; Nurlailah Nurlailah
Medical Laboratory Technology Journal Vol. 9 No. 1 (2023): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v9i1.506

Dracontomelon dao (Blanco) Merr. & Rofe is one of the medicinal plants for the community. This study aims to analyze the ethanol extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) using the LC-HRMS Phytochemical Test and determine its antibacterial activity. Leaf Dracontomelon dao (Blanco) Merr. & Rofe originates from Hulu Sungai Utara, South Kalimantan, Indonesia, macerated with 96% ethanol. Antibacterial test by diffusion was carried out using the well method. LC-HRMS (Liquid Chromatography - High-Resolution Mass Spectrometry) test using the Instrument Thermo Scientific™ Dionex™ Ultimate 3000 RSLCnano UHPLC coupled with Thermo Scientific™ Q Exactive™ High-Resolution Mass Spectrometer. The content of chemical compounds from the ethanol extract, namely flavonoids 125.5 ± 0.433 mgEQ/g, alkaloids (%) 33.945 ± 0.781, saponins (%) 33.093 ± 0.755, tannins (mg/ml) 0.069 ± 0.003, the highest content was triterpenoids 669.8 ± 2,000(mg/ml). The best inhibition of E coli growth with the largest inhibition zone was 24 mm at a 500 mg/ml concentration. The active compounds contained in the leaves of Dracontomelon dao (Blanco) Merr. & Rolfe are quercetin-3β-D-glucoside, anacardic acid, D-glucosamine, azelaic acid, choline, astragalin, quercetin, luteolin, syringic acid. The active ingredient of the ethanol extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) has the potential as an antibacterial and anti-inflammatory that can be added to pharmaceutical preparations. Keywords: Dracontomelon dao (Blanco) Merr. & Rofe; Phytochemical Analysis; Liquid Chromatography - High-Resolution Mass Spectrometry

Co-Authors Anny Thuraidah Anny Thuraidah Dini Aulia Azmi Dinna Rakhmina Erfan Roebiakto Herlena Fitriani Neni Oktiyani Noor Hikmah Damayanti Novina Kusumawardhani Ratih Dewi Dwiyanti Ratih Dewi Dwiyanti

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