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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal EduBio Tropika Dentino: Jurnal Kedokteran Gigi Jurnal Ilmiah Mahasiswa Pendidikan Biologi International Journal of Multidisciplinary: Applied Business and Education Research Jurnal Rekayasa Kimia & Lingkungan Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education)
Supriatno Supriatno
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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Journal : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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In vivo gene therapy with plasmid DNA-Tuberous sclerosis complex-2 (TSC-2) in an oral squamous cell carcinoma Supriatno Supriatno
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 37, No 02 (2005)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Tuberous sclerosis complex (TSC) is an inherited syndrome in which affected individuals are at increased risk for developing benign tumors. Mutation of the TSC-2 gene encoding tuberin on chromo-some 16p13.3 give rise to the clinical disorder of tuberous sclerosis characterized by the development of hamartomas.Objectives: In the present study, I conducted to examine whether overexpression of TSC-2 can affect the growth of human oral squamous cell carcinoma (OSCC) which have different expression level of p27K'P'. Methods: I constructed an expression vector containing sense-oriented rat TSC-2 cDNA with pcDNA3.1 (Invitrogen), and transfected to oral squamous cell carcinoma (B88) to regulate the expression of TSC-2 gene in each transfectant. Western blot analysis was carried out to determine the protein level of TSC-2. Therefore, Tumor volume and body weight of nude mice was examined using tumorigenicity assay.Results: Overexpression of TSC-2 exerted the growth inhibitory effect of oral squamous cell carcinoma. Sense-oriented B88-TSC-2 cancer cells, have high expression of p27K'P1. Moreover, tumor induced by TSC2 up-regulated transfectans become much smaller than those of control cells.Conclusion: overexpression of TSC-2 may exert the antitumor effect on oral squamous cell carcinoma through p27K'P' induction. Key word: TSC-2 - transfection - oral squamous cell carcinoma - pcDNA3.1 - mutation. 
Co-Authors Ali Sarong Andi Ulfa Tenri Pada Arlette Setiawan Audia Anda Rini Cut Nurmaliah Cutia Emelda Dina Mellina Putri Djufri Djufri Dwi Nowo Martono EKA SAFITRI Eka Safitri Hafnati Rahmatan Haruki Agustina Ilham Maulana Ira Mursyida Ismul Huda Julia Julia KHAIRI SUHUD Khairi Suhud Khairil Khairil Khairuna Khairuna* Lelifajri Lelifajri M Ali S Millati Ahya Muhibbuddin Muhibbuddin mulyati mulyati NAZARUDDIN NAZARUDDIN Nazaruddin Nazaruddin Rahmadi Rahmadi Rinaldi Idroes Risyandi Anwar Rusmawarni Rusmawarni Salmaniah Salmaniah Unang Supratman Uswatunnisa Uswatunnisa Wardiah Wardiah