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Supriatno Supriatno
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Dentistry Education Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Neuroscience

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BIOACTIVE COMPOUNDS OF RASAMALA (ALTINGIA EXCELSA NORNHA) LEAVES AS C-MYC PROTO ONCOGENE EXPRESSION SUPPRESSOR OF HUMAN TONGUE CANCER CELL IN VITRO Risyandi Anwar; Arlette Setiawan; Supriatno Supriatno; Unang Supratman
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5379

Abstract

Background: Tongue cancer is a common neoplasm found in oral cavity. It is characterized by aggressive cell growth, poor prognosis and being the cause of mortality. Objectives: to discover bioactive compounds of Rasamala leaves which possess an activity to inhibit SP-C1 human tongue cancer cell proliferation by reducing the expression of c-Myc proto oncogene. Methods: This is an experimental laboratory study using SP-C1 human tongue cancer cell. Separation of bioactive compounds from Rasamala leaves ethyl acetate extract was using various chromatography techniques guided by antiproliferative assay. Results: Two compounds were produced consisting of kaempferol (1) and quercetin (2). Compound 1 and 2 were tested to assess antiproliferative activity of kaempferol and quercetin upon SP-C1tongue cancer cell. IC50 values obtained from antiproliferative assay of each compound were 0.72 and 0.70 ug/ml respectively. Data analysis using ANCOVA test attained a significant value of α=0.05 and proceeded for probit analysis. The activity of  compound 1 and 2 was tested on c-Myc proto oncogene and it was acquired that compound 1 and 2 can suppress c-myc proto oncogene expression. Conclusion: Rasamala compounds consist of kaempferol (1) and quercetin (2) which possess an activity as tongue cancer cell proliferation inhibitor by reducing c-myc proto oncogene expression.
TINGKAT AKUMULASI MERKURI (HG) PADA ORGAN TUBUH KELAS GASTROPODA DI KAWASAN PERAIRAN SUNGAI SIKULAT KECAMATAN SAWANG KABUPATEN ACEH SELATAN Cutia Emelda; Supriatno Supriatno; Ali Sarong
Jurnal Edubio Tropika Vol 5, No 1 (2017): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan tingkat akumulasi merkuri (Hg) pada organ tubuh kelas Gastropoda yang terdapat di kawasan Perairan Sungai Sikulat Kecamatan Sawang Kabupaten AcehSelatan. Penelitian dilakukan di Sungai Sikulat Kecamatan Sawang Kabupaten Aceh Selatan, pada bulan Mei-Agustus 2015. Metode yang digunakan untuk menetapkan lokasi penelitian adalah metode survei dengan teknik purposive random sampling, sehingga ditetapkan 5 stasiun penelitian. Sampel Gastropoda yang ditemukan dianalisis merkurinya pada bagian kepala dan bagian badan dengan metode Spektrofotometri Serapan Atom (SSA). Tingkat akumulasi merkuri pada bagian kepala dan badan Gastropoda dianalisis dengan uji t. Hasil penelitian menunjukkan bahwa rerata akumulasi merkuri pada bagian kepala 0,679 ppm dan pada bagian badan 1,541 ppm. Hasil uji t untuk perbedaan tingkat akumulasi merkuri pada bagian kepala dan badan dihasilkan t-hitung = 3,657 dengan t tabel = 2,228. Kesimpulan pada penelitian ini adalah terdapat perbedaan tingkat akumulasi merkuri pada bagian kepala dengan bagian badan Gastropoda di Perairan Sungai Sikulat Kecamatan Sawang Kabupaten Aceh Selatan.
Effect of Salinity on Growth and Vitamin C Content of Aloe Vera (Aloe vera L.) Uswatunnisa Uswatunnisa; Wardiah Wardiah; Supriatno Supriatno
Jurnal Ilmiah Mahasiswa Pendidikan Biologi Vol 2, No 2 (2017): Mei 2017
Publisher : Jurnal Ilmiah Mahasiswa Pendidikan Biologi

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Abstract

Study about "Effect of Salinity on Growth and Vitamin C Content of Aloe (Aloe vera L.) on August-September 2016 in Garden of Agriculture Faculty and Biology Laboratory of Educational Faculty, Syiah Kuala University. The aim of the study is to determine the effect of salinity on growth and Vitamin C content of aloe vera. Study used a Completely Randomized Design (CRD) with the provision of NaCl treatment with different concentrations are: P0 = 0 mM, 150 mM = P1, P2 = 200 mM, 250 mM P3. Parameters that were measured are plant height, leaf number, leaf length, leaf thickness and content of vitamin C in the stem of aloe vera. Data were analyzed by analysis of variance (ANOVA). Further test used in plant height, leaf length and leaf thickness was LSD (Least Significance Difference ) and a further test used in the amount of leaves and content of vitamin C are a test of Duncan. The results showed that there is a real effect (F-count ≥ F 0.05) on the growth of aloe vera on the parameters plant height, leaf number, leaf length, leaf thickness and content of vitamin C. It is concluded that treatment of different salinity give negatively effect for growth, but positively effect on vitamin C content.Keywords: Growth, salinity, Vitamin C, Aloe vera L.
Phytochemical Screening and Antibacterial Test of Ethanolic Extract of Kawista (Limonia acidissima L.) from Aceh Besar against Escherichia coli Audia Anda Rini; Supriatno Supriatno; Hafnati Rahmatan
Jurnal Ilmiah Mahasiswa Pendidikan Biologi Vol 2, No 1 (2017): Pebruari 2017
Publisher : Jurnal Ilmiah Mahasiswa Pendidikan Biologi

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Abstract

Kawista is one of plant that is used as traditional medicine. One of the utilization of kawista is the consumtion of this unripe fruit to cure diarrhea. Etanolic extract of this fruit contains phytochemical components that has antibacterial activity. Study about Phytochemical Screening and Antibacterial Test of Ethanolic Extract of Kawista from Aceh Besar against Escherichia coli has been conducted on June 27 until July 14, 2016. The aims of the study is to determine phytochemical contents of ethanolic extract of fruit pulp of kawista and the effect of various concentrations against the growth of E. coli. Kawista which is used in the study gained from Aceh Besar. Phytochemical test showed that ethanolic extract of fruit pulp of kawista contain various active compounds such as alkaloids, saponins, tannins, triterpenoids, and polyphenols. E. coli that is used in this study were obtained from Microbiology Laboratory of Veterinary Faculty, Syiah Kuala University. The method used in the study is laboratory experiment using a quantitative type of Completely Randomized Design (CRD), which are divided into 7 groups, 5 treatment groups (ethanolic extract of fruit pulp of kawista) and 2 control groups using aquades (negative control) and streptomycin (positive control). Data analysis was performed by using ANOVA (Analysis of Variance) and then proceed with the test of HSD (Honestly Significant Difference) at α 5%. Antibacterial test results showed ethanolic extract of fruit pulp of kawista could inhibit the growth of E. coli (317.5 2.85). The highest inhibitory zone obtained at 500.000 ppm, with an average diameter of 16.141 mm. Conclusion of the study is showing that the extract of kawista pulps has antibacterial activity against E. coli bacterial growth. The higher concentration of the extract, the greater antibacterial activity against E. coli bacterial growth. Keywords: Ethanolic extract of kawista, antibacterial, Escherichia coli.
Penerapan Model Discovery Learning dengan Pemanfaatan Virtual Laboratory untuk Meningkatkan Keterampilan Proses Sains dan Hasil Belajar Peserta Didik pada Materi Sistem Ekskresi Khairuna Khairuna*; Hafnati Rahmatan; M Ali Sarong; Supriatno Supriatno; Andi Ulfa Tenri Pada
Jurnal Pendidikan Sains Indonesia Vol 9, No 2 (2021): APRIL 2021
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jpsi.v9i2.18875

Abstract

Practical skills play an important role in learning to achieve maximum learning outcomes. Lack of involvement in skills activities has an impact on learning outcomes achieved by students.This study aims to analyze the improvement of science process skills and student learning outcomes by applying the discovery learning model and the use of a virtual laboratory. The research method was a quasi-experimental design with a pretest-posttest control group design. The population was 165 students of class XI IPA who came from two schools. The research sample was 113 students, 57 experimental group and 56 control group. This research was conducted in MAN 1 and MAS Oemar Diyan Aceh Besar, Aceh, Indonesia. The research was conducted in the even semester of the 2019/2020 Academic Year. The parameters measured were science process skills and learning outcomes, data collection instruments were multiple choice questions with pretest-postest techniques. Science process skills data and learning outcomes were analyzed using parametric statistics using the independent sample t test. The results showed the correlation between science process skills and student learning outcomes was obtained r = 0.842, this indicates that between science process skills and student learning outcomes has a positive corelation with strong interpretation after being taught with the application of discovery learning models and the use of virtual laboratory on excretory system topic
In vivo gene therapy with plasmid DNA-Tuberous sclerosis complex-2 (TSC-2) in an oral squamous cell carcinoma Supriatno Supriatno
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 37, No 02 (2005)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Tuberous sclerosis complex (TSC) is an inherited syndrome in which affected individuals are at increased risk for developing benign tumors. Mutation of the TSC-2 gene encoding tuberin on chromo-some 16p13.3 give rise to the clinical disorder of tuberous sclerosis characterized by the development of hamartomas.Objectives: In the present study, I conducted to examine whether overexpression of TSC-2 can affect the growth of human oral squamous cell carcinoma (OSCC) which have different expression level of p27K'P'. Methods: I constructed an expression vector containing sense-oriented rat TSC-2 cDNA with pcDNA3.1 (Invitrogen), and transfected to oral squamous cell carcinoma (B88) to regulate the expression of TSC-2 gene in each transfectant. Western blot analysis was carried out to determine the protein level of TSC-2. Therefore, Tumor volume and body weight of nude mice was examined using tumorigenicity assay.Results: Overexpression of TSC-2 exerted the growth inhibitory effect of oral squamous cell carcinoma. Sense-oriented B88-TSC-2 cancer cells, have high expression of p27K'P1. Moreover, tumor induced by TSC2 up-regulated transfectans become much smaller than those of control cells.Conclusion: overexpression of TSC-2 may exert the antitumor effect on oral squamous cell carcinoma through p27K'P' induction. Key word: TSC-2 - transfection - oral squamous cell carcinoma - pcDNA3.1 - mutation. 
Acceptance analysis students using technology acceptance model on students of biology education program of Syiah Kuala University and Ar-raniry State Islamic University Millati Ahya; Ismul Huda; Supriatno Supriatno
Jurnal Edubio Tropika Vol 6, No 2 (2018): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

This research uses TAM (technology acceptance model)model with six constructs that are used are computer self-efficacy (CSE), perceived ease of use (PEOU), perceived usefulness (PU), attitude toward technology use (attitude Towards using technology / AT), intention to use (IU), and Frequency of Use (FU). This model is then used to examine which is an e-learning technology used in Biology Education Department of Syiah Kuala University and UIN Ar-Raniry. This study aims to determine the relationship between the factors that affect the acceptance and use of ebelajar.web.id at Syiah Kuala University and UIN Ar-Raniry. The type of research used in this research is explanatory research with data analysis technique using Part Least Square (PLS) approach. The method used to collect data is survey method. Data obtained from respondents who are students of Biology Education Faculty of Teacher Training and Education Universitas Syiah Kuala and Biology Education Faculty of Tarbiyah and Teacher Training of Ar-Raniry State Islamic University in the second semester of academic year 2016/2017 taking Information Technology and Computer and Ecology Course and environmental issues. The sample was 99 samples. The results of the data analysis were obtained as follows: (1) The CSE construct has significant effect on the PU construct; (2) CSE construct has significant effect on PEU construct; (3) PEU construction has significant effect on PU construct; (4) PEU construction has significant effect on construct A PU; (5) PEU construction has significant effect on IU construct; (6) PU construction has significant effect to construct A; (7) construct A has significant effect on IU construct. The conclusion in this research is that there are factors influencing to the acceptance level of learning technology using TAM model.
Application of the nature exploration approach based discovery learning model on students learning critical thinking skills and student achievement on plant world in SMA Negeri unggul Banda Aceh Julia Julia; Khairil Khairil; Supriatno Supriatno
Jurnal Edubio Tropika Vol 6, No 2 (2018): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

The purpose of this study was to determine the effect of the application of the nature exploration approach based discovery learning model on students learning critical thinking skills and student achievement. Data were collected in January until March 2017. The method used in this study is an quasi experimental method with pretest posttest control group design. Populations in this research are all student in class X MIPAat SMA Negeri Unggul Kota Banda Aceh is 854 student. The research was conducted in two schools: SMA Unggul Negeri 2 Boarding School and SMA Negeri 10 Fajar Harapan is 161 student. The instrument used was a tests to assess critical thinking skills and student achievement. Data analysis used independent sample t-test and Mann Whitney U Test with SPSS 17.0 for Windows at 0.05 significance level. The result showed that of significant critical thinking skills and students achievement 0,05 is significant 0,000 0,05. Conclusion the application of the nature exploration approach based discovery learning can significant effect that an increase in the ability of critical thinking skills and student achievement in the plant world at SMA Negeri Unggul Banda Aceh.Keywords: The Nature Exploration Approach, Discovery Learning and Plant World.
The effectiveness of the ethanol extracts of avocado leaves larvicide against Aedes aegypti and Culex quinquefasciatus larvae mortality Dina Mellina Putri; M Ali S; Supriatno Supriatno
Jurnal Edubio Tropika Vol 6, No 1 (2018): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

This research aims to find out the influence of the ethanol extracts of avocado leaves against larvae mortality of Aedes aegypti. This research was carried out in July 2014 until June 2015. The method usedis Complete Random design experiment method consists of seven treatments and four replicates.Concentrations of ethanol extract of the leaves of the avocado are 1000 ppm, 2000 ppm, 3000 and 4000 ppm,ppm 5000 ppm. Analysis of the influence of the ethanol extracts of avocado leaves against mortality of larvae is performed with ANOVA test, while the LC50 is performed with probit test. ANOVA test results of Aedesaegypti larvae mortality obtained a value of Fcount = 1597.72 Ftable(0.05) = 2.57 and Culex quinquefasciatus larvae mortality retrieved the value Fhitung = 1127.19 Ftabel(0.05) = 2.57. The result of LC50 analyzes at 72 hours observation showed the concentration of ethanol extract of avocado leaves that can kill 50% Aedes aegyptilarvae is 732,84 ppm and Culex quinquefasciatus larvae is 961,75 ppm. The conclusion obtained is the administering of ethanol extracts of avocado leaves significantly affect the mortality of the larvae of Aedesaegypti and Culex quinquefasciatus, thus it is potential to be used as natural larvicide.Keywords: Larvicide, Avocado leaves, Aedes aegypti and Culex quinquefasciatus
Penerapan strategi inkuiri terbimbing dan peta konsep dalam mengurangi miskonsepsi pada materi jaringan tumbuhan Ira Mursyida; Muhibbuddin Muhibbuddin; Supriatno Supriatno
Jurnal Edubio Tropika Vol 5, No 2 (2017): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

Penelitian bertujuan mengetahui penerapan strategi pembelajaran inkuiri terbimbing dan peta konsep terhadap pengurangan miskonsepsi mahasiswa pada materi jaringan tumbuhan. Pengambilan datadilakukan pada tanggal 10 februari sampai dengan 16 april 2016. Metode yang digunakan dalam penelitian ini adalah metode eksperimen dengan design pretest postest control group. Populasi penelitian seluruh mahasiswa STKIP Bina Bangsa Meulaboh jurusan Biologi berjumlah 166 mahasiswa. Sampel diambil denganteknik random sampling, berjumlah 40 mahasiswa, terdiri dari kelas eksperimen dan kelas kontrol. Instrumen yang digunakan adalah soal pilihan ganda model CRI (Certainty of Response Index). Hasil penelitian menunjukkan bahwa mahasiswa mengalami miskonsepsi pada semua sub materi saat pemberian pretes yaitu jaringan meristem (52%); jaringan pelindung (53%); jaringan dasar (62%); jaringan pembuluh (59%), sedangkan saat pemberian postes, jaringan meristem (18%); jaringan pelindung (19%); jaringan dasar (22%); jaringan pembuluh (12%). Simpulan penelitian ini adalah terdapat pengurangan miskonsepsi materi jaringan tumbuhan melalui penerapan strategi pembelajaran inkuiri terbimbing dan peta konsep.Kata Kunci: Strategi Inkuiri Terbimbing, Peta Konsep dan Miskonsepsi.
Co-Authors Ali Sarong Andi Ulfa Tenri Pada Anwar, Risyandi Arlette Setiawan Audia Anda Rini Cut Nurmaliah Cutia Emelda Dina Mellina Putri Djufri Djufri Eka Safitri Hafnati Rahmatan Ilham Maulana Ira Mursyida Ismul Huda Julia Julia Khairi Suhud Khairil Khairil Khairuna Khairuna* Lelifajri Lelifajri M Ali S M Ali Sarong Millati Ahya Muhibbuddin Muhibbuddin mulyati mulyati Nazaruddin Nazaruddin Rahmadi Rahmadi Rinaldi Idroes Rinaldi Idroes Rusmawarni Rusmawarni Salmaniah Salmaniah Unang Supratman Uswatunnisa Uswatunnisa Wardiah Wardiah