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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal EduBio Tropika Dentino: Jurnal Kedokteran Gigi Jurnal Ilmiah Mahasiswa Pendidikan Biologi International Journal of Multidisciplinary: Applied Business and Education Research Jurnal Rekayasa Kimia & Lingkungan Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education)
Supriatno Supriatno
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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The effectiveness of the ethanol extracts of avocado leaves larvicide against Aedes aegypti and Culex quinquefasciatus larvae mortality Dina Mellina Putri; M Ali S; Supriatno Supriatno
Jurnal Edubio Tropika Vol 6, No 1 (2018): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

This research aims to find out the influence of the ethanol extracts of avocado leaves against larvae mortality of Aedes aegypti. This research was carried out in July 2014 until June 2015. The method usedis Complete Random design experiment method consists of seven treatments and four replicates.Concentrations of ethanol extract of the leaves of the avocado are 1000 ppm, 2000 ppm, 3000 and 4000 ppm,ppm 5000 ppm. Analysis of the influence of the ethanol extracts of avocado leaves against mortality of larvae is performed with ANOVA test, while the LC50 is performed with probit test. ANOVA test results of Aedesaegypti larvae mortality obtained a value of Fcount = 1597.72 Ftable(0.05) = 2.57 and Culex quinquefasciatus larvae mortality retrieved the value Fhitung = 1127.19 Ftabel(0.05) = 2.57. The result of LC50 analyzes at 72 hours observation showed the concentration of ethanol extract of avocado leaves that can kill 50% Aedes aegyptilarvae is 732,84 ppm and Culex quinquefasciatus larvae is 961,75 ppm. The conclusion obtained is the administering of ethanol extracts of avocado leaves significantly affect the mortality of the larvae of Aedesaegypti and Culex quinquefasciatus, thus it is potential to be used as natural larvicide.Keywords: Larvicide, Avocado leaves, Aedes aegypti and Culex quinquefasciatus
Penerapan strategi inkuiri terbimbing dan peta konsep dalam mengurangi miskonsepsi pada materi jaringan tumbuhan Ira Mursyida; Muhibbuddin Muhibbuddin; Supriatno Supriatno
Jurnal Edubio Tropika Vol 5, No 2 (2017): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

Penelitian bertujuan mengetahui penerapan strategi pembelajaran inkuiri terbimbing dan peta konsep terhadap pengurangan miskonsepsi mahasiswa pada materi jaringan tumbuhan. Pengambilan datadilakukan pada tanggal 10 februari sampai dengan 16 april 2016. Metode yang digunakan dalam penelitian ini adalah metode eksperimen dengan design pretest postest control group. Populasi penelitian seluruh mahasiswa STKIP Bina Bangsa Meulaboh jurusan Biologi berjumlah 166 mahasiswa. Sampel diambil denganteknik random sampling, berjumlah 40 mahasiswa, terdiri dari kelas eksperimen dan kelas kontrol. Instrumen yang digunakan adalah soal pilihan ganda model CRI (Certainty of Response Index). Hasil penelitian menunjukkan bahwa mahasiswa mengalami miskonsepsi pada semua sub materi saat pemberian pretes yaitu jaringan meristem (52%); jaringan pelindung (53%); jaringan dasar (62%); jaringan pembuluh (59%), sedangkan saat pemberian postes, jaringan meristem (18%); jaringan pelindung (19%); jaringan dasar (22%); jaringan pembuluh (12%). Simpulan penelitian ini adalah terdapat pengurangan miskonsepsi materi jaringan tumbuhan melalui penerapan strategi pembelajaran inkuiri terbimbing dan peta konsep.Kata Kunci: Strategi Inkuiri Terbimbing, Peta Konsep dan Miskonsepsi.
PENERAPAN MODEL PEMBELAJARAN DISCOVERY LEARNINGMATERI JAMUR TERHADAP PEMAHAMAN KONSEP PESERTA DIDIK Salmaniah Salmaniah; Cut Nurmaliah; Supriatno Supriatno
Jurnal Edubio Tropika Vol 4, No 2 (2016): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

Penyampaian materi ajar yang diterapkan oleh guru masih kurang efektif sehingga siswa menjadi pasif. Oleh karena itu, untuk menciptakan peserta didik yang aktif dan kreatif dalam kegiatan pembelajaranperlu diterapkan model pembelajaran discovery learning. Adapun tujuan penelitian ini untuk mengetahui pemahaman konsep peserta didik, melalui penerapan model pembelajaran discovery learning dan penelitian ini dilaksanakan pada bulan Januari 2016. Metode penelitian yang digunakan adalah metode eksperimen (experimental research) dengan desain penelitian Pretest-posttest Control Group Design. Pada kelaseksperimen menggunakan model pembelajaran discovery learning dan kelas kontrol menggunakan pembelajaran konvensional. Teknik pengumpulan data menggunakan tes pilihan berganda, lembar observasi,dan dokumentasi. Uji hipotesis menggunakan uji t (t test). Hasil uji hipotesis penerapan model pembelajaran discovery learning untuk pemahaman konsep adalah t hitung 2,18, sedangkan t tabel yaitu 1,990 (α = 0,05). Hal ini menunjukkan bahwa nilai t hitung lebih besar dari pada nilai t tabel, sehingga nilai rata-rata antara kelas eksperimendan kelas kontrol signifikan atau berbeda nyata. Penelitian ini menyimpulkan bahwa penerapan model pembelajaran discovery learning materi jamur berpengaruh dalam meningkatkan pemahaman konsep siswa, hal tersebut terlihat pada perolehan rata-rata pretest, posttest, N-gain pada kelas eksperimen dan kelas kontrol.
PENGARUH BERBAGAI KONSENTRASI PUPUK ORGANIK CAIR DARI URIN SAPI DAN PUPUK HAYATI BIOBOOST TERHADAP PERTUMBUHAN TANAMAN STROBERI (Fragaria virginiana) Rusmawarni Rusmawarni; Djufri Djufri; Supriatno Supriatno
Jurnal Edubio Tropika Vol 4, No 2 (2016): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

Penelitian ini bertujuan mengetahui pengaruh berbagai konsentrasi pupuk organik cair dari urin sapi terhadap pertumbuhan tanaman stroberi (fragaria virginiana) dan mendapat konsentrasi yang tepat.Penelitian dilaksanakan di desa Jeget Ayu, kecamatan Jagong Jeget, kabupaten Aceh Tengah yang dilaksanakan pada tanggal 25 Juli sampai dengan 23 September 2015. Penelitian ini menggunakan RancanganAcak Lengkap (RAL) faktorial dengan empat ulangan. Faktor pertama adalah pupuk organik cair dengan taraf dosis 0 ml/polybag (S0), 10 ml/polybag (S1), 21 ml/polybag (S2) dan 32 ml/polybag (S3) dan faktor kedua adalah pupuk hayati Bioboost dengan taraf dosis 0 ml/polybag (B0), 10 ml/polybag (B1), 11 ml/polybag (B2) dan 21 ml/polybag (B3). Data hasil pengamatan dianalisis secara statistik dengan analisis varians pada taraf 5%, jika berpengaruh nyata maka dilanjutkan dengan uji wilayah berganda Duncan Multiple Range Test (DMRT) pada taraf 5%. Parameter yang diamati adalah panjang tangkai daun, jumlah daun dan jumlah bunga. Hasil penelitian menunjukkan bahwa Penggunaan pupuk organik cair dari urin sapi dan pupuk hayati Bioboost memberikan pengaruh nyata terhadap pertumbuhan tanaman stroberi (fragaria virginiana) dan kombinasi perlakuan S2B1 dengan pemberian pupuk organik cair dari urine sapi sebanyak 21 ml/polybag dan pupuk hayati Bioboost sebanyak 10 ml/polybag memberikan pengaruh yang lebih tinggi dibandingkan perlakuan lain.
Application of the nature exploration approach based discovery learning model on students learning critical thinking skills and student achievement on plant world in SMA Negeri unggul Banda Aceh Julia Julia; Khairil Khairil; Supriatno Supriatno
Jurnal Edubio Tropika Vol 6, No 2 (2018): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

The purpose of this study was to determine the effect of the application of the nature exploration approach based discovery learning model on students learning critical thinking skills and student achievement. Data were collected in January until March 2017. The method used in this study is an quasi experimental method with pretest posttest control group design. Populations in this research are all student in class X MIPAat SMA Negeri Unggul Kota Banda Aceh is 854 student. The research was conducted in two schools: SMA Unggul Negeri 2 Boarding School and SMA Negeri 10 Fajar Harapan is 161 student. The instrument used was a tests to assess critical thinking skills and student achievement. Data analysis used independent sample t-test and Mann Whitney U Test with SPSS 17.0 for Windows at 0.05 significance level. The result showed that of significant critical thinking skills and students achievement 0,05 is significant 0,000 0,05. Conclusion the application of the nature exploration approach based discovery learning can significant effect that an increase in the ability of critical thinking skills and student achievement in the plant world at SMA Negeri Unggul Banda Aceh.Keywords: The Nature Exploration Approach, Discovery Learning and Plant World.
Acceptance analysis students using technology acceptance model on students of biology education program of Syiah Kuala University and Ar-raniry State Islamic University Millati Ahya; Ismul Huda; Supriatno Supriatno
Jurnal Edubio Tropika Vol 6, No 2 (2018): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

This research uses TAM (technology acceptance model)model with six constructs that are used are computer self-efficacy (CSE), perceived ease of use (PEOU), perceived usefulness (PU), attitude toward technology use (attitude Towards using technology / AT), intention to use (IU), and Frequency of Use (FU). This model is then used to examine which is an e-learning technology used in Biology Education Department of Syiah Kuala University and UIN Ar-Raniry. This study aims to determine the relationship between the factors that affect the acceptance and use of ebelajar.web.id at Syiah Kuala University and UIN Ar-Raniry. The type of research used in this research is explanatory research with data analysis technique using Part Least Square (PLS) approach. The method used to collect data is survey method. Data obtained from respondents who are students of Biology Education Faculty of Teacher Training and Education Universitas Syiah Kuala and Biology Education Faculty of Tarbiyah and Teacher Training of Ar-Raniry State Islamic University in the second semester of academic year 2016/2017 taking Information Technology and Computer and Ecology Course and environmental issues. The sample was 99 samples. The results of the data analysis were obtained as follows: (1) The CSE construct has significant effect on the PU construct; (2) CSE construct has significant effect on PEU construct; (3) PEU construction has significant effect on PU construct; (4) PEU construction has significant effect on construct A PU; (5) PEU construction has significant effect on IU construct; (6) PU construction has significant effect to construct A; (7) construct A has significant effect on IU construct. The conclusion in this research is that there are factors influencing to the acceptance level of learning technology using TAM model.
TINGKAT AKUMULASI MERKURI (HG) PADA ORGAN TUBUH KELAS GASTROPODA DI KAWASAN PERAIRAN SUNGAI SIKULAT KECAMATAN SAWANG KABUPATEN ACEH SELATAN Cutia Emelda; Supriatno Supriatno; Ali Sarong
Jurnal Edubio Tropika Vol 5, No 1 (2017): Jurnal EduBio Tropika
Publisher : Jurnal Edubio Tropika

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Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan tingkat akumulasi merkuri (Hg) pada organ tubuh kelas Gastropoda yang terdapat di kawasan Perairan Sungai Sikulat Kecamatan Sawang Kabupaten AcehSelatan. Penelitian dilakukan di Sungai Sikulat Kecamatan Sawang Kabupaten Aceh Selatan, pada bulan Mei-Agustus 2015. Metode yang digunakan untuk menetapkan lokasi penelitian adalah metode survei dengan teknik purposive random sampling, sehingga ditetapkan 5 stasiun penelitian. Sampel Gastropoda yang ditemukan dianalisis merkurinya pada bagian kepala dan bagian badan dengan metode Spektrofotometri Serapan Atom (SSA). Tingkat akumulasi merkuri pada bagian kepala dan badan Gastropoda dianalisis dengan uji t. Hasil penelitian menunjukkan bahwa rerata akumulasi merkuri pada bagian kepala 0,679 ppm dan pada bagian badan 1,541 ppm. Hasil uji t untuk perbedaan tingkat akumulasi merkuri pada bagian kepala dan badan dihasilkan t-hitung = 3,657 dengan t tabel = 2,228. Kesimpulan pada penelitian ini adalah terdapat perbedaan tingkat akumulasi merkuri pada bagian kepala dengan bagian badan Gastropoda di Perairan Sungai Sikulat Kecamatan Sawang Kabupaten Aceh Selatan.
In vivo gene therapy with plasmid DNA-Tuberous sclerosis complex-2 (TSC-2) in an oral squamous cell carcinoma Supriatno Supriatno
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 37, No 02 (2005)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Tuberous sclerosis complex (TSC) is an inherited syndrome in which affected individuals are at increased risk for developing benign tumors. Mutation of the TSC-2 gene encoding tuberin on chromo-some 16p13.3 give rise to the clinical disorder of tuberous sclerosis characterized by the development of hamartomas.Objectives: In the present study, I conducted to examine whether overexpression of TSC-2 can affect the growth of human oral squamous cell carcinoma (OSCC) which have different expression level of p27K'P'. Methods: I constructed an expression vector containing sense-oriented rat TSC-2 cDNA with pcDNA3.1 (Invitrogen), and transfected to oral squamous cell carcinoma (B88) to regulate the expression of TSC-2 gene in each transfectant. Western blot analysis was carried out to determine the protein level of TSC-2. Therefore, Tumor volume and body weight of nude mice was examined using tumorigenicity assay.Results: Overexpression of TSC-2 exerted the growth inhibitory effect of oral squamous cell carcinoma. Sense-oriented B88-TSC-2 cancer cells, have high expression of p27K'P1. Moreover, tumor induced by TSC2 up-regulated transfectans become much smaller than those of control cells.Conclusion: overexpression of TSC-2 may exert the antitumor effect on oral squamous cell carcinoma through p27K'P' induction. Key word: TSC-2 - transfection - oral squamous cell carcinoma - pcDNA3.1 - mutation. 
BIOACTIVE COMPOUNDS OF RASAMALA (ALTINGIA EXCELSA NORNHA) LEAVES AS C-MYC PROTO ONCOGENE EXPRESSION SUPPRESSOR OF HUMAN TONGUE CANCER CELL IN VITRO Risyandi Anwar; Arlette Setiawan; Supriatno Supriatno; Unang Supratman
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5379

Abstract

Background: Tongue cancer is a common neoplasm found in oral cavity. It is characterized by aggressive cell growth, poor prognosis and being the cause of mortality. Objectives: to discover bioactive compounds of Rasamala leaves which possess an activity to inhibit SP-C1 human tongue cancer cell proliferation by reducing the expression of c-Myc proto oncogene. Methods: This is an experimental laboratory study using SP-C1 human tongue cancer cell. Separation of bioactive compounds from Rasamala leaves ethyl acetate extract was using various chromatography techniques guided by antiproliferative assay. Results: Two compounds were produced consisting of kaempferol (1) and quercetin (2). Compound 1 and 2 were tested to assess antiproliferative activity of kaempferol and quercetin upon SP-C1tongue cancer cell. IC50 values obtained from antiproliferative assay of each compound were 0.72 and 0.70 ug/ml respectively. Data analysis using ANCOVA test attained a significant value of α=0.05 and proceeded for probit analysis. The activity of  compound 1 and 2 was tested on c-Myc proto oncogene and it was acquired that compound 1 and 2 can suppress c-myc proto oncogene expression. Conclusion: Rasamala compounds consist of kaempferol (1) and quercetin (2) which possess an activity as tongue cancer cell proliferation inhibitor by reducing c-myc proto oncogene expression.
ANALYSIS VEGETATION OF SHADE CORPSE FLOWER (Amorphophallus paeoniifolius (Dennst.) Nicholson) in PADANG TIJI SUB-DISTRICT of PIDIE DISTRICT Mulyati Mulyati; Djufri Djufri; Supriatno Supriatno
Jurnal Ilmiah Mahasiswa Pendidikan Biologi Vol 2, No 1 (2017): Pebruari 2017
Publisher : Jurnal Ilmiah Mahasiswa Pendidikan Biologi

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Abstract

ABSTRACTCorpse flower (Amorphophallus paeoniifolius) is one of the seasonal herbaceous plants including familia Araceae that can grow well in the shade. The shade of vegetation can be used as the corpse flower habitat preferences in the region, making it easier to know the population of the largest corpse flower under the shade of vegetation. This study aims to determine the type of vegetation that the shelter and to find out how the diversity index of vegetation corpse flower shade in the Padang Tiji sub-district of Pidie district. The Research was done by finding the whereabouts of the corpse flower in Padang Tiji to key persons at the altitude of 200, 400 and 600, so it can be done later retrieval corpse flower shade vegetation data using squares method. At each elevation laid five plots area of each plot was 10 m x 10 m. Based on the analysis of vegetation that houses the corpse flower, found as many as 20 species from 11 familia. Corpse flower is found in the shade of a tree Sterculia alata and Areca catechu, so that both species have the highest among other species on the third altitude. Additionally, Leea aequata and Eupatorium odoratum are also shade of vegetation that were dominant at the three altitudes for the habitus of shrubs / bushes. Thus, the corpse flower shade preferences can be known from the type of vegetation shading. Vegetation diversity index auspices of the corpse flower has a lower category either on habitus trees or shrubs / bushes.Keywords: the corpse flower, vegetation, shade, diversity index.
Co-Authors Ali Sarong Andi Ulfa Tenri Pada Arlette Setiawan Audia Anda Rini Cut Nurmaliah Cutia Emelda Dina Mellina Putri Djufri Djufri Dwi Nowo Martono EKA SAFITRI Eka Safitri Hafnati Rahmatan Haruki Agustina Ilham Maulana Ira Mursyida Ismul Huda Julia Julia KHAIRI SUHUD Khairi Suhud Khairil Khairil Khairuna Khairuna* Lelifajri Lelifajri M Ali S Millati Ahya Muhibbuddin Muhibbuddin mulyati mulyati NAZARUDDIN NAZARUDDIN Nazaruddin Nazaruddin Rahmadi Rahmadi Rinaldi Idroes Risyandi Anwar Rusmawarni Rusmawarni Salmaniah Salmaniah Unang Supratman Uswatunnisa Uswatunnisa Wardiah Wardiah